Restriction map for
pF1KB9778
(Circular) (Six-base) MAPSORT of: pF1KB9778.seq Check: 9857 from: 1 to: 5507
pF1KB9778 5507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1565 1565
Size: 5507
AccI GT'mk_AC
Cuts at: 222 1599 2545 222
Size: 1377 946 3184
Fragments arranged by size:
3184 1377 946
Acc65I G'GTAC_C
Cuts at: 2523 2523
Size: 5507
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 630 1142 1465 3332 3764 4117 4779 542
Size: 88 512 323 1867 432 353 662 1270
Fragments arranged by size:
1867 1270 662 512 432 353 323 88
AflIII A'CryG_T
Cuts at: 1993 2859 4659 4940 1993
Size: 866 1800 281 2560
Fragments arranged by size:
2560 1800 866 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1361 1393 1361
Size: 32 5475
AlwNI CAG_nnn'CTG
Cuts at: 1083 1120 2812 4250 1083
Size: 37 1692 1438 2340
Fragments arranged by size:
2340 1692 1438 37
ApaLI G'TGCA_C
Cuts at: 249 4345 249
Size: 4096 1411
ApoI r'AATT_y
Cuts at: 111 255 508 837 1126 1745 1759 2401 2511
Size: 144 253 329 289 619 14 642 110
Cuts at: 2511 4860 5116 111
Size: 2349 256 502
Fragments arranged by size:
2349 642 619 502 329 289 256 253
144 110 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 2527 2527
Size: 5507
BamHI G'GATC_C
Cuts at: 2532 2532
Size: 5507
BanI G'GyrC_C
Cuts at: 2523 2937 3185 3220 2523
Size: 414 248 35 4810
Fragments arranged by size:
4810 414 248 35
BanII G_rGCy'C
Cuts at: 2521 3551 2521
Size: 1030 4477
BbeI G_GCGC'C
Cuts at: 2941 3189 2941
Size: 248 5259
BbsI GAAGACnn'nnnn_
Cuts at: 530 1643 530
Size: 1113 4394
BciVI GTATCCnnnnn_n'
Cuts at: 1884 3405 4461 5507 1884
Size: 1521 1056 1046 1884
Fragments arranged by size:
1884 1521 1056 1046
BclI T'GATC_A
Cuts at: 140 1217 3027 140
Size: 1077 1810 2620
Fragments arranged by size:
2620 1810 1077
BfrBI ATG'CAT
Cuts at: 1228 1228
Size: 5507
BglII A'GATC_T
Cuts at: 609 1922 3022 5059 609
Size: 1313 1100 2037 1057
Fragments arranged by size:
2037 1313 1100 1057
BlpI GC'TnA_GC
Cuts at: 710 2030 2619 710
Size: 1320 589 3598
Fragments arranged by size:
3598 1320 589
Bme1580I G_kGCm'C
Cuts at: 253 3132 3225 4349 253
Size: 2879 93 1124 1411
Fragments arranged by size:
2879 1411 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2719 2800 3135 5279 2719
Size: 81 335 2144 2947
Fragments arranged by size:
2947 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3874 3874
Size: 5507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 833 927 983 320
Size: 513 94 56 4844
Fragments arranged by size:
4844 513 94 56
Bpu10I CC'TnA_GC
Cuts at: 715 715
Size: 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 2683 3562 4276 4574 409
Size: 2274 879 714 298 1342
Fragments arranged by size:
2274 1342 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5211 5211
Size: 5507
BsaAI yAC'GTr
Cuts at: 2219 2860 2219
Size: 641 4866
BsaBI GATnn'nnATC
Cuts at: 6 477 3021 5058 6
Size: 471 2544 2037 455
Fragments arranged by size:
2544 2037 471 455
BsaHI Gr'CG_yC
Cuts at: 2938 3186 3888 5384 2938
Size: 248 702 1496 3061
Fragments arranged by size:
3061 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2696 3217 3895 4306 4453 4727 2696
Size: 521 678 411 147 274 3476
Fragments arranged by size:
3476 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1361 1391 1685 1715 1361
Size: 30 294 30 5153
Fragments arranged by size:
5153 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 642 642
Size: 5507
BseYI C'CCAG_C
Cuts at: 2147 2932 3690 4355 4763 2147
Size: 785 758 665 408 2891
Fragments arranged by size:
2891 785 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 729 1593 2073 275
Size: 454 864 480 3709
Fragments arranged by size:
3709 864 480 454
BsiEI CG_ry'CG
Cuts at: 104 3049 3095 4325 104
Size: 2945 46 1230 1286
Fragments arranged by size:
2945 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 253 776 2521 3299 3489 4349 253
Size: 523 1745 778 190 860 1411
Fragments arranged by size:
1745 1411 860 778 523 190
BsmI GAATG_Cn'
Cuts at: 284 1507 1615 2427 284
Size: 1223 108 812 3364
Fragments arranged by size:
3364 1223 812 108
Bsp1286I G_dGCh'C
Cuts at: 253 776 2521 3132 3225 3299 3489 3551 4349
Size: 523 1745 611 93 74 190 62 798
Cuts at: 4349 253
Size: 1411
Fragments arranged by size:
1745 1411 798 611 523 190 93 74
62
BspHI T'CATG_A
Cuts at: 1539 2379 3939 1539
Size: 840 1560 3107
Fragments arranged by size:
3107 1560 840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1565 2020 2557 2771 3073 3454 1565
Size: 455 537 214 302 381 3618
Fragments arranged by size:
3618 537 455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3799 3853 5337 5502 50
Size: 3749 54 1484 165 55
Fragments arranged by size:
3749 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1209 1587 1935 3419 1209
Size: 378 348 1484 3297
Fragments arranged by size:
3297 1484 378 348
BsrFI r'CCGG_y
Cuts at: 2151 3505 3686 2151
Size: 1354 181 3972
Fragments arranged by size:
3972 1354 181
BsrGI T'GTAC_A
Cuts at: 1028 1966 1028
Size: 938 4569
BssSI C'ACGA_G
Cuts at: 3778 4486 3778
Size: 708 4799
BstAPI GCAn_nnn'nTGC
Cuts at: 1763 2884 1763
Size: 1121 4386
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5507
BstXI CCAn_nnnn'nTGG
Cuts at: 128 128
Size: 5507
BstYI r'GATC_y
Cuts at: 1 446 609 1792 1922 1971 2532 2999 3022
Size: 445 163 1183 130 49 561 467 23
Cuts at: 3022 3357 3603 3909 3921 4007 5059 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1183 1052 561 467 449 445 335 306
246 163 130 86 49 23 12
BstZ17I GTA'TAC
Cuts at: 223 223
Size: 5507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 1875 2750 1875
Size: 875 4632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 192 227 443 478 192
Size: 35 216 35 5221
Fragments arranged by size:
5221 216 35 35
DraI TTT'AAA
Cuts at: 191 1437 2506 191
Size: 1246 1069 3192
Fragments arranged by size:
3192 1246 1069
DraIII CAC_nnn'GTG
Cuts at: 298 298
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 3213 4557 3213
Size: 1344 4163
EaeI y'GGCC_r
Cuts at: 1932 3092 3266 3657 3684 5029 1932
Size: 1160 174 391 27 1345 2410
Fragments arranged by size:
2410 1345 1160 391 174 27
EagI C'GGCC_G
Cuts at: 3092 3092
Size: 5507
EarI CTCTTCn'nnn_
Cuts at: 1720 3530 3740 1720
Size: 1810 210 3487
Fragments arranged by size:
3487 1810 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3715 4457 4603 5317 3715
Size: 742 146 714 3905
Fragments arranged by size:
3905 742 714 146
EcoICRI GAG'CTC
Cuts at: 2519 2519
Size: 5507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 542 630 833 927 983 1142 1465 3332
Size: 222 88 203 94 56 159 323 1867
Cuts at: 3332 3764 4117 4779 320
Size: 432 353 662 1048
Fragments arranged by size:
1867 1048 662 432 353 323 222 203
159 94 88 56
EcoO109I rG'GnC_Cy
Cuts at: 2646 2646
Size: 5507
EcoRI G'AATT_C
Cuts at: 2511 2511
Size: 5507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 3288 3288
Size: 5507
HaeII r_GCGC'y
Cuts at: 2941 3189 4419 5190 2941
Size: 248 1230 771 3258
Fragments arranged by size:
3258 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1685 1717 2155 2187 3349 3379 3381 3411 1685
Size: 32 438 32 1162 30 2 30 3781
Fragments arranged by size:
3781 1162 438 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1629 2196 2546 1629
Size: 567 350 4590
Fragments arranged by size:
4590 567 350
HindIII A'AGCT_T
Cuts at: 2362 2362
Size: 5507
HpaI GTT'AAC
Cuts at: 1629 1629
Size: 5507
KasI G'GCGC_C
Cuts at: 2937 3185 2937
Size: 248 5259
KpnI G_GTAC'C
Cuts at: 2527 2527
Size: 5507
MfeI C'AATT_G
Cuts at: 2300 2300
Size: 5507
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 474 795 2181 4266 4450 474
Size: 321 1386 2085 184 1531
Fragments arranged by size:
2085 1531 1386 321 184
MscI TGG'CCA
Cuts at: 3268 3268
Size: 5507
MslI CAynn'nnrTG
Cuts at: 1046 1560 2097 3496 3623 1046
Size: 514 537 1399 127 2930
Fragments arranged by size:
2930 1399 537 514 127
MspA1I CmG'CkG
Cuts at: 2618 2932 3292 4076 4321 2618
Size: 314 360 784 245 3804
Fragments arranged by size:
3804 784 360 314 245
NaeI GCC'GGC
Cuts at: 3688 3688
Size: 5507
NarI GG'CG_CC
Cuts at: 2938 3186 2938
Size: 248 5259
NgoMIV G'CCGG_C
Cuts at: 3686 3686
Size: 5507
NsiI A_TGCA'T
Cuts at: 1230 1230
Size: 5507
NspI r_CATG'y
Cuts at: 1877 2090 2560 3060 4663 1877
Size: 213 470 500 1603 2721
Fragments arranged by size:
2721 1603 500 470 213
PciI A'CATG_T
Cuts at: 4659 4659
Size: 5507
PflMI CCAn_nnn'nTGG
Cuts at: 241 856 1000 241
Size: 615 144 4748
Fragments arranged by size:
4748 615 144
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1361 1393 3953 3985 1361
Size: 32 2560 32 2883
Fragments arranged by size:
2883 2560 32 32
PsiI TTA'TAA
Cuts at: 2456 2456
Size: 5507
PstI C_TGCA'G
Cuts at: 1657 1684 2554 3239 1657
Size: 27 870 685 3925
Fragments arranged by size:
3925 870 685 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 2932 3292 2932
Size: 360 5147
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 2521 2521
Size: 5507
SalI G'TCGA_C
Cuts at: 2544 2544
Size: 5507
SapI GCTCTTCn'nnn_
Cuts at: 3530 3740 3530
Size: 210 5297
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 1899 5024 1899
Size: 3125 2382
SfcI C'TryA_G
Cuts at: 33 1653 1680 2550 3235 4203 4394 33
Size: 1620 27 870 685 968 191 1146
Fragments arranged by size:
1620 1146 968 870 685 191 27
SfoI GGC'GCC
Cuts at: 2939 3187 2939
Size: 248 5259
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2529 2529
Size: 5507
SmlI C'TyrA_G
Cuts at: 424 2662 3577 4291 4553 424
Size: 2238 915 714 262 1378
Fragments arranged by size:
2238 1378 915 714 262
SnaBI TAC'GTA
Cuts at: 2219 2219
Size: 5507
SpeI A'CTAG_T
Cuts at: 67 1894 67
Size: 1827 3680
SphI G_CATG'C
Cuts at: 2560 3060 2560
Size: 500 5007
SspI AAT'ATT
Cuts at: 1098 1359 1098
Size: 261 5246
StuI AGG'CCT
Cuts at: 1446 1446
Size: 5507
StyI C'CwwG_G
Cuts at: 2016 2120 2641 2995 2016
Size: 104 521 354 4528
Fragments arranged by size:
4528 521 354 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 3090 3223 3682 3891 233
Size: 2857 133 459 209 1849
Fragments arranged by size:
2857 1849 459 209 133
TatI w'GTAC_w
Cuts at: 1028 1484 1897 1966 5022 1028
Size: 456 413 69 3056 1513
Fragments arranged by size:
3056 1513 456 413 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 963 995 5024 5056 963
Size: 32 4029 32 1414
Fragments arranged by size:
4029 1414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1007 3304 1007
Size: 2297 3210
XbaI T'CTAG_A
Cuts at: 2538 2538
Size: 5507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 242 3002 242
Size: 2760 2747
XmaI C'CCGG_G
Cuts at: 2527 2527
Size: 5507
XmnI GAAnn'nnTTC
Cuts at: 1127 1350 2045 1127
Size: 223 695 4589
Fragments arranged by size:
4589 695 223
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsiWI BsmBI BspEI BssHII BstEII Bsu36I BtgI ClaI
EcoNI EcoRV FseI FspAI NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SwaI XhoI ZraI