Restriction map for
pF1KB9760
(Circular) (Six-base) MAPSORT of: pF1KB9760.seq Check: 3439 from: 1 to: 5330
pF1KB9760 5330 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 366 366
Size: 5330
AccI GT'mk_AC
Cuts at: 722 2368 722
Size: 1646 3684
Acc65I G'GTAC_C
Cuts at: 1467 2346 1467
Size: 879 4451
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 3155 3587 4334 4602 1888
Size: 1267 432 747 268 2616
Fragments arranged by size:
2616 1267 747 432 268
AfeI AGC'GCT
Cuts at: 739 752 739
Size: 13 5317
AflIII A'CryG_T
Cuts at: 2682 3786 4763 2682
Size: 1104 977 3249
Fragments arranged by size:
3249 1104 977
AgeI A'CCGG_T
Cuts at: 3718 3718
Size: 5330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2023 2023
Size: 5330
AlwNI CAG_nnn'CTG
Cuts at: 280 848 1444 1645 1836 2635 4202 280
Size: 568 596 201 191 799 1567 1408
Fragments arranged by size:
1567 1408 799 596 568 201 191
ApaI G_GGCC'C
Cuts at: 298 501 633 1132 1180 1332 1379 1586 298
Size: 203 132 499 48 152 47 207 4042
Fragments arranged by size:
4042 499 207 203 152 132 48 47
ApaLI G'TGCA_C
Cuts at: 1654 4100 1654
Size: 2446 2884
ApoI r'AATT_y
Cuts at: 2334 4683 4939 2334
Size: 2349 256 2725
Fragments arranged by size:
2725 2349 256
AscI GG'CGCG_CC
Cuts at: 896 896
Size: 5330
AseI AT'TA_AT
Cuts at: 20 20
Size: 5330
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 297 306 316 328 370 547 1431 1485 2350
Size: 9 10 12 42 177 884 54 865
Cuts at: 2350 297
Size: 3277
Fragments arranged by size:
3277 884 865 177 54 42 12 10
9
AvrII C'CTAG_G
Cuts at: 1947 1947
Size: 5330
BamHI G'GATC_C
Cuts at: 301 2355 301
Size: 2054 3276
BanI G'GyrC_C
Cuts at: 1204 1392 1467 1951 2052 2298 2346 2760 3008
Size: 188 75 484 101 246 48 414 248
Cuts at: 3008 3043 1204
Size: 35 3491
Fragments arranged by size:
3491 484 414 248 246 188 101 75
48 35
BanII G_rGCy'C
Cuts at: 298 501 536 633 1015 1132 1180 1332 1379
Size: 203 35 97 382 117 48 152 47
Cuts at: 1379 1510 1586 2315 2344 3374 298
Size: 131 76 729 29 1030 2254
Fragments arranged by size:
2254 1030 729 382 203 152 131 117
97 76 48 47 35 29
BbeI G_GCGC'C
Cuts at: 2056 2764 3012 2056
Size: 708 248 4374
Fragments arranged by size:
4374 708 248
BbvCI CC'TCA_GC
Cuts at: 290 2188 2267 290
Size: 1898 79 3353
Fragments arranged by size:
3353 1898 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 985 1019 1737 1771 985
Size: 34 718 34 4544
Fragments arranged by size:
4544 718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1502 3228 3989 5330 1502
Size: 1726 761 1341 1502
Fragments arranged by size:
1726 1502 1341 761
BclI T'GATC_A
Cuts at: 2850 2850
Size: 5330
BglI GCCn_nnn'nGGC
Cuts at: 419 470 505 628 1336 1434 419
Size: 51 35 123 708 98 4315
Fragments arranged by size:
4315 708 123 98 51 35
BglII A'GATC_T
Cuts at: 2845 4882 2845
Size: 2037 3293
BlpI GC'TnA_GC
Cuts at: 2442 2442
Size: 5330
Bme1580I G_kGCm'C
Cuts at: 298 501 633 1132 1180 1209 1332 1379 1586
Size: 203 132 499 48 29 123 47 207
Cuts at: 1586 1658 2955 3048 4104 298
Size: 72 1297 93 1056 1524
Fragments arranged by size:
1524 1297 1056 499 207 203 132 123
93 72 48 47 29
BmrI ACTGGGnnnn_n'
Cuts at: 979 1202 2542 2623 2958 5102 979
Size: 223 1340 81 335 2144 1207
Fragments arranged by size:
2144 1340 1207 335 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 5330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 988 1152 1312 1980 2275 388
Size: 600 164 160 668 295 3443
Fragments arranged by size:
3443 668 600 295 164 160
Bpu10I CC'TnA_GC
Cuts at: 290 1504 2188 2267 290
Size: 1214 684 79 3353
Fragments arranged by size:
3353 1214 684 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 739 2506 3385 3877 4175 447
Size: 292 1767 879 492 298 1602
Fragments arranged by size:
1767 1602 879 492 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 1237 1312 5034 1237
Size: 75 3722 1533
Fragments arranged by size:
3722 1533 75
BsaAI yAC'GTr
Cuts at: 469 2683 469
Size: 2214 3116
BsaBI GATnn'nnATC
Cuts at: 6 923 2844 4881 6
Size: 917 1921 2037 455
Fragments arranged by size:
2037 1921 917 455
BsaHI Gr'CG_yC
Cuts at: 311 2053 2761 3009 3711 5207 311
Size: 1742 708 248 702 1496 434
Fragments arranged by size:
1742 1496 708 702 434 248
BsaWI w'CCGG_w
Cuts at: 875 2519 3040 3718 3992 4139 4550 875
Size: 1644 521 678 274 147 411 1655
Fragments arranged by size:
1655 1644 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1023 1053 1288 1318 1352 1382 1023
Size: 30 235 30 34 30 4971
Fragments arranged by size:
4971 235 34 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 208 727 1053 1108 1309 1378 2323
Size: 3 3 519 326 55 201 69 945
Cuts at: 2323 202
Size: 3209
Fragments arranged by size:
3209 945 519 326 201 69 55 3
3
BseYI C'CCAG_C
Cuts at: 148 1107 1222 1918 2160 2285 2755 3513 4090
Size: 959 115 696 242 125 470 758 577
Cuts at: 4090 4586 148
Size: 496 892
Fragments arranged by size:
959 892 758 696 577 496 470 242
125 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 1946 1795
Size: 151 5179
BsiEI CG_ry'CG
Cuts at: 104 182 233 257 423 458 639 876 2090
Size: 78 51 24 166 35 181 237 1214
Cuts at: 2090 2872 2918 4126 104
Size: 782 46 1208 1308
Fragments arranged by size:
1308 1214 1208 782 237 181 166 78
51 46 35 24
BsiHKAI G_wGCw'C
Cuts at: 536 1118 1658 2344 3122 3312 4104 536
Size: 582 540 686 778 190 792 1762
Fragments arranged by size:
1762 792 778 686 582 540 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1830 1870 1830
Size: 40 5290
Bsp1286I G_dGCh'C
Cuts at: 298 501 536 633 1015 1118 1132 1180 1209
Size: 203 35 97 382 103 14 48 29
Cuts at: 1209 1332 1379 1510 1586 1658 2315 2344 2955
Size: 123 47 131 76 72 657 29 611
Cuts at: 2955 3048 3122 3312 3374 4104 298
Size: 93 74 190 62 730 1524
Fragments arranged by size:
1524 730 657 611 382 203 190 131
123 103 97 93 76 74 72 62
48 47 35 29 29 14
BspEI T'CCGG_A
Cuts at: 4550 4550
Size: 5330
BspHI T'CATG_A
Cuts at: 4506 4506
Size: 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 366 608 828 1538 2380 2594 2896 3277 366
Size: 242 220 710 842 214 302 381 2419
Fragments arranged by size:
2419 842 710 381 302 242 220 214
BsrBI CCG'CTC
Cuts at: 50 627 1473 3622 3676 5160 5325 50
Size: 577 846 2149 54 1484 165 55
Fragments arranged by size:
2149 1484 846 577 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3242 3242
Size: 5330
BsrFI r'CCGG_y
Cuts at: 141 418 539 820 860 1033 3328 3509 3718
Size: 277 121 281 40 173 2295 181 209
Cuts at: 3718 141
Size: 1753
Fragments arranged by size:
2295 1753 281 277 209 181 173 121
40
BssHII G'CGCG_C
Cuts at: 202 489 491 603 605 607 641 896 202
Size: 287 2 112 2 2 34 255 4636
Fragments arranged by size:
4636 287 255 112 34 2 2 2
BssSI C'ACGA_G
Cuts at: 3601 3959 3601
Size: 358 4972
BstAPI GCAn_nnn'nTGC
Cuts at: 222 280 1645 2707 222
Size: 58 1365 1062 2845
Fragments arranged by size:
2845 1365 1062 58
BstBI TT'CG_AA
Cuts at: 3691 3691
Size: 5330
BstEII G'GTnAC_C
Cuts at: 1560 1560
Size: 5330
BstXI CCAn_nnnn'nTGG
Cuts at: 1023 1023
Size: 5330
BstYI r'GATC_y
Cuts at: 1 301 2355 2822 2845 3180 3426 4438 4524
Size: 300 2054 467 23 335 246 1012 86
Cuts at: 4524 4536 4882 1
Size: 12 346 449
Fragments arranged by size:
2054 1012 467 449 346 335 300 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 959 1229 1895 2210 959
Size: 270 666 315 4079
Fragments arranged by size:
4079 666 315 270
BtgI C'CryG_G
Cuts at: 107 350 633 768 1496 2084 107
Size: 243 283 135 728 588 3353
Fragments arranged by size:
3353 728 588 283 243 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3280 3461 3624 3280
Size: 181 163 4986
Fragments arranged by size:
4986 181 163
BtsI GCAGTG_nn'
Cuts at: 445 659 1118 1706 2573 445
Size: 214 459 588 867 3202
Fragments arranged by size:
3202 867 588 459 214
DraI TTT'AAA
Cuts at: 2329 2329
Size: 5330
DrdI GACnn_nn'nnGTC
Cuts at: 3036 3894 3036
Size: 858 4472
EaeI y'GGCC_r
Cuts at: 230 254 420 455 636 809 1703 1730 1769
Size: 24 166 35 181 173 894 27 39
Cuts at: 1769 1880 2087 2915 3089 3480 3507 4852 230
Size: 111 207 828 174 391 27 1345 708
Fragments arranged by size:
1345 894 828 708 391 207 181 174
173 166 111 39 35 27 27 24
EagI C'GGCC_G
Cuts at: 230 254 420 455 636 2087 2915 230
Size: 24 166 35 181 1451 828 2645
Fragments arranged by size:
2645 1451 828 181 166 35 24
EarI CTCTTCn'nnn_
Cuts at: 179 3353 3563 179
Size: 3174 210 1946
Fragments arranged by size:
3174 1946 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 981 3538 3848 3994 5140 981
Size: 2557 310 146 1146 1171
Fragments arranged by size:
2557 1171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 534 2342 534
Size: 1808 3522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 988 1152 1312 1888 1980 2275 3155 3587
Size: 600 164 160 576 92 295 880 432
Cuts at: 3587 4334 4602 388
Size: 747 268 1116
Fragments arranged by size:
1116 880 747 600 576 432 295 268
164 160 92
EcoNI CCTnn'n_nnAGG
Cuts at: 1159 1274 1360 1534 1570 1898 1159
Size: 115 86 174 36 328 4591
Fragments arranged by size:
4591 328 174 115 86 36
EcoO109I rG'GnC_Cy
Cuts at: 294 498 591 963 1128 1129 1311 1328 1368
Size: 204 93 372 165 1 182 17 40
Cuts at: 1368 1375 1376 1582 1583 1642 1833 2172 2469
Size: 7 1 206 1 59 191 339 297
Cuts at: 2469 294
Size: 3155
Fragments arranged by size:
3155 372 339 297 206 204 191 182
165 93 59 40 17 7 1 1
1
EcoRI G'AATT_C
Cuts at: 2334 2334
Size: 5330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 5330
FspI TGC'GCA
Cuts at: 673 3111 673
Size: 2438 2892
HaeII r_GCGC'y
Cuts at: 400 465 741 754 804 2056 2764 3012 4034
Size: 65 276 13 50 1252 708 248 1022
Cuts at: 4034 5013 400
Size: 979 717
Fragments arranged by size:
1252 1022 979 717 708 276 248 65
50 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 928 960 1023 1055 1288 1320 1352 1384 3172
Size: 32 63 32 233 32 32 32 1788
Cuts at: 3172 3202 3204 3234 928
Size: 30 2 30 3024
Fragments arranged by size:
3024 1788 233 63 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2250 2369 2250
Size: 119 5211
KasI G'GCGC_C
Cuts at: 2052 2760 3008 2052
Size: 708 248 4374
Fragments arranged by size:
4374 708 248
KpnI G_GTAC'C
Cuts at: 1471 2350 1471
Size: 879 4451
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1286 2240 4001 4185 1286
Size: 954 1761 184 2431
Fragments arranged by size:
2431 1761 954 184
MscI TGG'CCA
Cuts at: 811 1732 3091 811
Size: 921 1359 3050
Fragments arranged by size:
3050 1359 921
MslI CAynn'nnrTG
Cuts at: 1072 1816 1888 3319 3446 1072
Size: 744 72 1431 127 2956
Fragments arranged by size:
2956 1431 744 127 72
MspA1I CmG'CkG
Cuts at: 277 516 635 687 770 1003 1100 1182 1593
Size: 239 119 52 83 233 97 82 411
Cuts at: 1593 1633 1695 1984 2086 2441 2755 3115 4128
Size: 40 62 289 102 355 314 360 1013
Cuts at: 4128 4373 277
Size: 245 1234
Fragments arranged by size:
1234 1013 411 360 355 314 289 245
239 233 119 102 97 83 82 62
52 40
NaeI GCC'GGC
Cuts at: 143 420 3511 143
Size: 277 3091 1962
Fragments arranged by size:
3091 1962 277
NarI GG'CG_CC
Cuts at: 2053 2761 3009 2053
Size: 708 248 4374
Fragments arranged by size:
4374 708 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5330
NgoMIV G'CCGG_C
Cuts at: 141 418 3509 141
Size: 277 3091 1962
Fragments arranged by size:
3091 1962 277
NotI GC'GGCC_GC
Cuts at: 636 636
Size: 5330
NspI r_CATG'y
Cuts at: 1821 2383 2883 3790 1821
Size: 562 500 907 3361
Fragments arranged by size:
3361 907 562 500
PasI CC'CwG_GG
Cuts at: 1008 1155 1008
Size: 147 5183
PciI A'CATG_T
Cuts at: 3786 3786
Size: 5330
PflMI CCAn_nnn'nTGG
Cuts at: 1357 1578 1357
Size: 221 5109
PfoI T'CCnGG_A
Cuts at: 1262 1344 1262
Size: 82 5248
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 4469
Size: 32 5298
PpuMI rG'GwC_Cy
Cuts at: 591 963 1368 1833 591
Size: 372 405 465 4088
Fragments arranged by size:
4088 465 405 372
PspOMI G'GGCC_C
Cuts at: 294 497 629 1128 1176 1328 1375 1582 294
Size: 203 132 499 48 152 47 207 4042
Fragments arranged by size:
4042 499 207 203 152 132 48 47
PstI C_TGCA'G
Cuts at: 590 1592 1638 1694 2377 3062 590
Size: 1002 46 56 683 685 2858
Fragments arranged by size:
2858 1002 685 683 56 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 1593 1633 1695 2755 3115 1593
Size: 40 62 1060 360 3808
Fragments arranged by size:
3808 1060 360 62 40
RsrII CG'GwC_CG
Cuts at: 878 3525 878
Size: 2647 2683
SacI G_AGCT'C
Cuts at: 536 2344 536
Size: 1808 3522
SacII CC_GC'GG
Cuts at: 636 771 2087 636
Size: 135 1316 3879
Fragments arranged by size:
3879 1316 135
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 3353 3563 3353
Size: 210 5120
SbfI CC_TGCA'GG
Cuts at: 590 2377 590
Size: 1787 3543
ScaI AGT'ACT
Cuts at: 4847 4847
Size: 5330
SfcI C'TryA_G
Cuts at: 33 586 1588 1634 1690 2373 3058 4051 4242
Size: 553 1002 46 56 683 685 993 191
Cuts at: 4242 33
Size: 1121
Fragments arranged by size:
1121 1002 993 685 683 553 191 56
46
SfiI GGCCn_nnn'nGGCC
Cuts at: 419 505 628 419
Size: 86 123 5121
Fragments arranged by size:
5121 123 86
SfoI GGC'GCC
Cuts at: 2054 2762 3010 2054
Size: 708 248 4374
Fragments arranged by size:
4374 708 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 299 308 318 1433 1487 2352 299
Size: 9 10 1115 54 865 3277
Fragments arranged by size:
3277 1115 865 54 10 9
SmlI C'TyrA_G
Cuts at: 426 547 754 2485 3400 3892 4154 426
Size: 121 207 1731 915 492 262 1602
Fragments arranged by size:
1731 1602 915 492 262 207 121
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 1821 2383 2883 1821
Size: 562 500 4268
Fragments arranged by size:
4268 562 500
StuI AGG'CCT
Cuts at: 760 1165 1904 760
Size: 405 739 4186
Fragments arranged by size:
4186 739 405
StyI C'CwwG_G
Cuts at: 107 1356 1947 2273 2315 2464 2818 107
Size: 1249 591 326 42 149 354 2619
Fragments arranged by size:
2619 1249 591 354 326 149 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 2913 3046 3505 3714 196
Size: 2717 133 459 209 1812
Fragments arranged by size:
2717 1812 459 209 133
TatI w'GTAC_w
Cuts at: 4845 4845
Size: 5330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4847 4879 4847
Size: 32 5298
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1254 1393 1554 2825 1254
Size: 139 161 1271 3759
Fragments arranged by size:
3759 1271 161 139
XhoI C'TCGA_G
Cuts at: 547 547
Size: 5330
XmaI C'CCGG_G
Cuts at: 297 306 316 1431 1485 2350 297
Size: 9 10 1115 54 865 3277
Fragments arranged by size:
3277 1115 865 54 10 9
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI BaeI BbsI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI