Restriction map for
pF1KB9743
(Circular) (Six-base) MAPSORT of: pF1KB9743.seq Check: 5595 from: 1 to: 4685
pF1KB9743 4685 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 545 545
Size: 4685
AccI GT'mk_AC
Cuts at: 1723 1723
Size: 4685
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 1679 4525 1679
Size: 2846 1839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 877 2510 2942 3689 3957 157
Size: 720 1633 432 747 268 885
Fragments arranged by size:
1633 885 747 720 432 268
AfeI AGC'GCT
Cuts at: 925 1015 925
Size: 90 4595
AflIII A'CryG_T
Cuts at: 2037 3141 4118 2037
Size: 1104 977 2604
Fragments arranged by size:
2604 1104 977
AgeI A'CCGG_T
Cuts at: 869 3073 869
Size: 2204 2481
AleI CACnn'nnGTG
Cuts at: 881 881
Size: 4685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 557 557
Size: 4685
AlwNI CAG_nnn'CTG
Cuts at: 465 1990 3557 465
Size: 1525 1567 1593
Fragments arranged by size:
1593 1567 1525
ApaI G_GGCC'C
Cuts at: 531 1161 1306 1473 531
Size: 630 145 167 3743
Fragments arranged by size:
3743 630 167 145
ApaLI G'TGCA_C
Cuts at: 3455 3455
Size: 4685
ApoI r'AATT_y
Cuts at: 1689 4038 4294 1689
Size: 2349 256 2080
Fragments arranged by size:
2349 2080 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 304 406 694 1705 304
Size: 102 288 1011 3284
Fragments arranged by size:
3284 1011 288 102
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1458 1561 1701 2115 2363 2398 1458
Size: 103 140 414 248 35 3745
Fragments arranged by size:
3745 414 248 140 103 35
BanII G_rGCy'C
Cuts at: 531 650 1091 1161 1306 1473 1651 1699 2729
Size: 119 441 70 145 167 178 48 1030
Cuts at: 2729 531
Size: 2487
Fragments arranged by size:
2487 1030 441 178 167 145 119 70
48
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbvCI CC'TCA_GC
Cuts at: 349 349
Size: 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 685 719 90
Size: 34 561 34 4056
Fragments arranged by size:
4056 561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3344 4685 2583
Size: 761 1341 2583
Fragments arranged by size:
2583 1341 761
BclI T'GATC_A
Cuts at: 2205 2205
Size: 4685
BglI GCCn_nnn'nGGC
Cuts at: 1211 1211
Size: 4685
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 443 531 1161 1257 1306 1463 1473 2310 2403
Size: 88 630 96 49 157 10 837 93
Cuts at: 2403 3459 443
Size: 1056 1669
Fragments arranged by size:
1669 1056 837 630 157 96 93 88
49 10
BmrI ACTGGGnnnn_n'
Cuts at: 437 732 1393 1897 1978 2313 4457 437
Size: 295 661 504 81 335 2144 665
Fragments arranged by size:
2144 665 661 504 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 419 868 921 245
Size: 174 449 53 4009
Fragments arranged by size:
4009 449 174 53
Bpu10I CC'TnA_GC
Cuts at: 349 349
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3232 3530 1861
Size: 879 492 298 3016
Fragments arranged by size:
3016 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1404 4389 1404
Size: 2985 1700
BsaAI yAC'GTr
Cuts at: 972 2038 972
Size: 1066 3619
BsaBI GATnn'nnATC
Cuts at: 6 775 1168 2199 4236 6
Size: 769 393 1031 2037 455
Fragments arranged by size:
2037 1031 769 455 393
BsaHI Gr'CG_yC
Cuts at: 1495 1586 2116 2364 3066 4562 1495
Size: 91 530 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 530 248 91
BsaWI w'CCGG_w
Cuts at: 869 1874 2395 3073 3347 3494 3905 869
Size: 1005 521 678 274 147 411 1649
Fragments arranged by size:
1649 1005 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 537 537
Size: 4685
BseYI C'CCAG_C
Cuts at: 189 225 487 620 1027 1179 1628 2110 2868
Size: 36 262 133 407 152 449 482 758
Cuts at: 2868 3445 3941 189
Size: 577 496 933
Fragments arranged by size:
933 758 577 496 482 449 407 262
152 133 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 517 199
Size: 318 4367
BsiEI CG_ry'CG
Cuts at: 104 874 2227 2273 3481 104
Size: 770 1353 46 1208 1308
Fragments arranged by size:
1353 1308 1208 770 46
BsiHKAI G_wGCw'C
Cuts at: 213 650 1091 1699 2477 2667 3459 213
Size: 437 441 608 778 190 792 1439
Fragments arranged by size:
1439 792 778 608 441 437 190
Bsp1286I G_dGCh'C
Cuts at: 213 443 531 650 1091 1161 1257 1306 1463
Size: 230 88 119 441 70 96 49 157
Cuts at: 1463 1473 1651 1699 2310 2403 2477 2667 2729
Size: 10 178 48 611 93 74 190 62
Cuts at: 2729 3459 213
Size: 730 1439
Fragments arranged by size:
1439 730 611 441 230 190 178 157
119 96 93 88 74 70 62 49
48 10
BspEI T'CCGG_A
Cuts at: 3905 3905
Size: 4685
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 545 599 832 1735 1949 2251 2632 437
Size: 108 54 233 903 214 302 381 2490
Fragments arranged by size:
2490 903 381 302 233 214 108 54
BsrBI CCG'CTC
Cuts at: 50 129 2977 3031 4515 4680 50
Size: 79 2848 54 1484 165 55
Fragments arranged by size:
2848 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 2597
Size: 4685
BsrFI r'CCGG_y
Cuts at: 869 1665 2683 2864 3073 869
Size: 796 1018 181 209 2481
Fragments arranged by size:
2481 1018 796 209 181
BssSI C'ACGA_G
Cuts at: 340 1075 2956 3314 340
Size: 735 1881 358 1711
Fragments arranged by size:
1881 1711 735 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2062 2062
Size: 4685
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 609 1473 609
Size: 864 3821
BstYI r'GATC_y
Cuts at: 1 860 1710 2177 2200 2535 2781 3793 3879
Size: 859 850 467 23 335 246 1012 86
Cuts at: 3879 3891 4237 1
Size: 12 346 449
Fragments arranged by size:
1012 859 850 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 276 276
Size: 4685
BtgI C'CryG_G
Cuts at: 741 1056 1580 741
Size: 315 524 3846
Fragments arranged by size:
3846 524 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 463 1033 1120 1287 1928 463
Size: 570 87 167 641 3220
Fragments arranged by size:
3220 641 570 167 87
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DraIII CAC_nnn'GTG
Cuts at: 972 1068 972
Size: 96 4589
DrdI GACnn_nn'nnGTC
Cuts at: 2391 3249 2391
Size: 858 3827
EaeI y'GGCC_r
Cuts at: 744 1059 1340 1528 2270 2444 2835 2862 4207
Size: 315 281 188 742 174 391 27 1345
Cuts at: 4207 744
Size: 1222
Fragments arranged by size:
1345 1222 742 391 315 281 188 174
27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1394 2893 3203 3349 4495 1394
Size: 1499 310 146 1146 1584
Fragments arranged by size:
1584 1499 1146 310 146
EcoICRI GAG'CTC
Cuts at: 648 1089 1697 648
Size: 441 608 3636
Fragments arranged by size:
3636 608 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 245 419 868 877 921 2510 2942 3689
Size: 88 174 449 9 44 1589 432 747
Cuts at: 3689 3957 157
Size: 268 885
Fragments arranged by size:
1589 885 747 449 432 268 174 88
44 9
EcoO109I rG'GnC_Cy
Cuts at: 426 527 528 1157 1198 1303 1318 1469 1470
Size: 101 1 629 41 105 15 151 1
Cuts at: 1470 1824 426
Size: 354 3287
Fragments arranged by size:
3287 629 354 151 105 101 41 15
1 1
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 1039 2466 1039
Size: 1427 3258
HaeII r_GCGC'y
Cuts at: 414 927 1017 2119 2367 3389 4368 414
Size: 513 90 1102 248 1022 979 731
Fragments arranged by size:
1102 1022 979 731 513 248 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1015 1047 2527 2557 2559 2589 1015
Size: 32 1480 30 2 30 3111
Fragments arranged by size:
3111 1480 32 30 30 2
HincII GTy'rAC
Cuts at: 675 1590 1724 675
Size: 915 134 3636
Fragments arranged by size:
3636 915 134
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 844 956 3356 3540 844
Size: 112 2400 184 1989
Fragments arranged by size:
2400 1989 184 112
MscI TGG'CCA
Cuts at: 1061 1342 2446 1061
Size: 281 1104 3300
Fragments arranged by size:
3300 1104 281
MslI CAynn'nnrTG
Cuts at: 881 2674 2801 881
Size: 1793 127 2765
Fragments arranged by size:
2765 1793 127
MspA1I CmG'CkG
Cuts at: 609 815 1098 1183 1353 1420 1520 1597 1796
Size: 206 283 85 170 67 100 77 199
Cuts at: 1796 2110 2470 3483 3728 609
Size: 314 360 1013 245 1566
Fragments arranged by size:
1566 1013 360 314 283 245 206 199
170 100 85 77 67
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 4685
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NcoI C'CATG_G
Cuts at: 741 1056 741
Size: 315 4370
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 4685
NspI r_CATG'y
Cuts at: 1738 2238 3145 1738
Size: 500 907 3278
Fragments arranged by size:
3278 907 500
PasI CC'CwG_GG
Cuts at: 1306 1473 1306
Size: 167 4518
PciI A'CATG_T
Cuts at: 3141 3141
Size: 4685
PflMI CCAn_nnn'nTGG
Cuts at: 367 972 367
Size: 605 4080
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PpuMI rG'GwC_Cy
Cuts at: 426 426
Size: 4685
PspOMI G'GGCC_C
Cuts at: 527 1157 1302 1469 527
Size: 630 145 167 3743
Fragments arranged by size:
3743 630 167 145
PstI C_TGCA'G
Cuts at: 829 1097 1732 2417 829
Size: 268 635 685 3097
Fragments arranged by size:
3097 685 635 268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 609 1098 1183 1420 1520 1597 2110 2470 609
Size: 489 85 237 100 77 513 360 2824
Fragments arranged by size:
2824 513 489 360 237 100 85 77
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 650 1091 1699 650
Size: 441 608 3636
Fragments arranged by size:
3636 608 441
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SexAI A'CCwGG_T
Cuts at: 659 659
Size: 4685
SfcI C'TryA_G
Cuts at: 33 825 1093 1728 2413 3406 3597 33
Size: 792 268 635 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 635 268 191
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 1840 2755 3247 3509 1840
Size: 915 492 262 3016
Fragments arranged by size:
3016 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
StyI C'CwwG_G
Cuts at: 566 741 1056 1819 2173 566
Size: 175 315 763 354 3078
Fragments arranged by size:
3078 763 354 315 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 181 979 1620 2268 2401 2860 3069 181
Size: 798 641 648 133 459 209 1797
Fragments arranged by size:
1797 798 648 641 459 209 133
TatI w'GTAC_w
Cuts at: 4200 4200
Size: 4685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 177 209 1054 1086 4202 4234 177
Size: 32 845 32 3116 32 628
Fragments arranged by size:
3116 845 628 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 670 2482 670
Size: 1812 2873
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1470 2180 114
Size: 1356 710 2619
Fragments arranged by size:
2619 1356 710
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI