Restriction map for
pF1KB9705
(Circular) (Six-base) MAPSORT of: pF1KB9705.seq Check: 7625 from: 1 to: 4118
pF1KB9705 4118 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 899 1156 899
Size: 257 3861
Acc65I G'GTAC_C
Cuts at: 767 1134 767
Size: 367 3751
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1943 2375 3122 3390 1943
Size: 432 747 268 2671
Fragments arranged by size:
2671 747 432 268
AfeI AGC'GCT
Cuts at: 447 455 447
Size: 8 4110
AflIII A'CryG_T
Cuts at: 590 890 1470 2574 3551 590
Size: 300 580 1104 977 1157
Fragments arranged by size:
1157 1104 977 580 300
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AhdI GACnn_n'nnGTC
Cuts at: 711 711
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1423 2990 1423
Size: 1567 2551
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 979 1122 3471 3727 979
Size: 143 2349 256 1370
Fragments arranged by size:
2349 1370 256 143
AscI GG'CGCG_CC
Cuts at: 373 373
Size: 4118
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 313 441 1138 313
Size: 128 697 3293
Fragments arranged by size:
3293 697 128
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 136 145 630 767 1134 1548 1796 1831 136
Size: 9 485 137 367 414 248 35 2423
Fragments arranged by size:
2423 485 414 367 248 137 35 9
BanII G_rGCy'C
Cuts at: 337 679 1132 2162 337
Size: 342 453 1030 2293
Fragments arranged by size:
2293 1030 453 342
BbeI G_GCGC'C
Cuts at: 140 149 1552 1800 140
Size: 9 1403 248 2458
Fragments arranged by size:
2458 1403 248 9
BciVI GTATCCnnnnn_n'
Cuts at: 663 2016 2777 4118 663
Size: 1353 761 1341 663
Fragments arranged by size:
1353 1341 761 663
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 406 695 1743 1836 2892 406
Size: 289 1048 93 1056 1632
Fragments arranged by size:
1632 1056 1048 289 93
BmgBI CAC'GTC
Cuts at: 591 591
Size: 4118
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 976 434
Size: 542 3576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1294 2173 2665 2963 520
Size: 774 879 492 298 1675
Fragments arranged by size:
1675 879 774 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 690 1471 690
Size: 781 3337
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 137 146 190 1549 1797 2499 3995 137
Size: 9 44 1359 248 702 1496 260
Fragments arranged by size:
1496 1359 702 260 248 44 9
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 949
Size: 30 4088
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 156 153
Size: 3 4115
BseYI C'CCAG_C
Cuts at: 390 405 456 558 1543 2301 2878 3374 390
Size: 15 51 102 985 758 577 496 1134
Fragments arranged by size:
1134 985 758 577 496 102 51 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 798 798
Size: 4118
BsiEI CG_ry'CG
Cuts at: 104 428 1660 1706 2914 104
Size: 324 1232 46 1208 1308
Fragments arranged by size:
1308 1232 1208 324 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
BsmI GAATG_Cn'
Cuts at: 240 534 240
Size: 294 3824
Bsp1286I G_dGCh'C
Cuts at: 337 406 679 695 1132 1743 1836 1910 2100
Size: 69 273 16 437 611 93 74 190
Cuts at: 2100 2162 2892 337
Size: 62 730 1563
Fragments arranged by size:
1563 730 611 437 273 190 93 74
69 62 16
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1382 1684 2065 1168
Size: 214 302 381 3221
Fragments arranged by size:
3221 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 139 618 2116 2297 2506 139
Size: 479 1498 181 209 1751
Fragments arranged by size:
1751 1498 479 209 181
BssHII G'CGCG_C
Cuts at: 373 373
Size: 4118
BssSI C'ACGA_G
Cuts at: 742 2389 2747 742
Size: 1647 358 2113
Fragments arranged by size:
2113 1647 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstYI r'GATC_y
Cuts at: 1 1143 1610 1633 1968 2214 3226 3312 3324
Size: 1142 467 23 335 246 1012 86 12
Cuts at: 3324 3670 1
Size: 346 449
Fragments arranged by size:
1142 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 204 572 758 107
Size: 97 368 186 3467
Fragments arranged by size:
3467 368 186 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 527 2068 2249 2412 527
Size: 1541 181 163 2233
Fragments arranged by size:
2233 1541 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DraIII CAC_nnn'GTG
Cuts at: 513 513
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 895 1824 2682 895
Size: 929 858 2331
Fragments arranged by size:
2331 929 858
EaeI y'GGCC_r
Cuts at: 425 620 1703 1877 2268 2295 3640 425
Size: 195 1083 174 391 27 1345 903
Fragments arranged by size:
1345 1083 903 391 195 174 27
EagI C'GGCC_G
Cuts at: 425 1703 425
Size: 1278 2840
EarI CTCTTCn'nnn_
Cuts at: 162 342 360 900 913 1059 2141 2351 162
Size: 180 18 540 13 146 1082 210 1929
Fragments arranged by size:
1929 1082 540 210 180 146 18 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 976 1943 2375 3122 3390 434
Size: 542 967 432 747 268 1162
Fragments arranged by size:
1162 967 747 542 432 268
EcoO109I rG'GnC_Cy
Cuts at: 317 836 1257 317
Size: 519 421 3178
Fragments arranged by size:
3178 519 421
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 140 149 439 449 457 1552 1800 2822 3801
Size: 9 290 10 8 1095 248 1022 979
Cuts at: 3801 140
Size: 457
Fragments arranged by size:
1095 1022 979 457 290 248 10 9
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 697 729 1093 1125 1960 1990 1992 2022 697
Size: 32 364 32 835 30 2 30 2793
Fragments arranged by size:
2793 835 364 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 136 145 1548 1796 136
Size: 9 1403 248 2458
Fragments arranged by size:
2458 1403 248 9
KpnI G_GTAC'C
Cuts at: 771 1138 771
Size: 367 3751
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 589 757 2107 2234 589
Size: 168 1350 127 2473
Fragments arranged by size:
2473 1350 168 127
MspA1I CmG'CkG
Cuts at: 206 268 289 424 430 494 1229 1543 1903
Size: 62 21 135 6 64 735 314 360
Cuts at: 1903 2916 3161 206
Size: 1013 245 1163
Fragments arranged by size:
1163 1013 735 360 314 245 135 64
62 21 6
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 137 146 1549 1797 137
Size: 9 1403 248 2458
Fragments arranged by size:
2458 1403 248 9
NcoI C'CATG_G
Cuts at: 107 758 107
Size: 651 3467
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NotI GC'GGCC_GC
Cuts at: 425 425
Size: 4118
NspI r_CATG'y
Cuts at: 894 1171 1671 2578 894
Size: 277 500 907 2434
Fragments arranged by size:
2434 907 500 277
PciI A'CATG_T
Cuts at: 890 2574 890
Size: 1684 2434
PfoI T'CCnGG_A
Cuts at: 775 775
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PmlI CAC'GTG
Cuts at: 690 690
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PpuMI rG'GwC_Cy
Cuts at: 317 836 317
Size: 519 3599
PstI C_TGCA'G
Cuts at: 423 435 944 1165 1850 423
Size: 12 509 221 685 2691
Fragments arranged by size:
2691 685 509 221 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 494 1543 1903 494
Size: 1049 360 2709
Fragments arranged by size:
2709 1049 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SacII CC_GC'GG
Cuts at: 207 207
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SanDI GG'GwC_CC
Cuts at: 836 836
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 342 2141 2351 342
Size: 1799 210 2109
Fragments arranged by size:
2109 1799 210
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 419 431 489 940 1161 1846 2839 3030
Size: 386 12 58 451 221 685 993 191
Cuts at: 3030 33
Size: 1121
Fragments arranged by size:
1121 993 685 451 386 221 191 58
12
SfoI GGC'GCC
Cuts at: 138 147 1550 1798 138
Size: 9 1403 248 2458
Fragments arranged by size:
2458 1403 248 9
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SgrAI Cr'CCGG_yG
Cuts at: 139 139
Size: 4118
SmaI CCC'GGG
Cuts at: 315 1140 315
Size: 825 3293
SmlI C'TyrA_G
Cuts at: 535 1273 2188 2680 2942 535
Size: 738 915 492 262 1711
Fragments arranged by size:
1711 915 738 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StuI AGG'CCT
Cuts at: 599 748 599
Size: 149 3969
StyI C'CwwG_G
Cuts at: 107 644 758 818 1252 1606 107
Size: 537 114 60 434 354 2619
Fragments arranged by size:
2619 537 434 354 114 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 426 1701 1834 2293 2502 426
Size: 1275 133 459 209 2042
Fragments arranged by size:
2042 1275 459 209 133
TatI w'GTAC_w
Cuts at: 581 809 3633 581
Size: 228 2824 1066
Fragments arranged by size:
2824 1066 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 313 1138 313
Size: 825 3293
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI