Restriction map for
pF1KB9701
(Circular) (Six-base) MAPSORT of: pF1KB9701.seq Check: 2286 from: 1 to: 4073
pF1KB9701 4073 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 272 272
Size: 4073
AccI GT'mk_AC
Cuts at: 1111 1111
Size: 4073
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1898 2330 3077 3345 688
Size: 1210 432 747 268 1416
Fragments arranged by size:
1416 1210 747 432 268
AfeI AGC'GCT
Cuts at: 757 757
Size: 4073
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 790 822 790
Size: 32 4041
AlwNI CAG_nnn'CTG
Cuts at: 264 1378 2945 264
Size: 1114 1567 1392
Fragments arranged by size:
1567 1392 1114
ApaI G_GGCC'C
Cuts at: 226 484 226
Size: 258 3815
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 1077 3426 3682 1077
Size: 2349 256 1468
Fragments arranged by size:
2349 1468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 192 213 436 454 1035 1093 192
Size: 21 223 18 581 58 3172
Fragments arranged by size:
3172 581 223 58 21 18
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 427 836 1089 1503 1751 1786 427
Size: 409 253 414 248 35 2714
Fragments arranged by size:
2714 414 409 253 248 35
BanII G_rGCy'C
Cuts at: 121 152 226 314 353 484 658 683 1012
Size: 31 74 88 39 131 174 25 329
Cuts at: 1012 1087 2117 121
Size: 75 1030 2077
Fragments arranged by size:
2077 1030 329 174 131 88 75 74
39 31 25
BbeI G_GCGC'C
Cuts at: 431 840 1507 1755 431
Size: 409 667 248 2749
Fragments arranged by size:
2749 667 409 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 831 865 831
Size: 34 4039
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 1593 1593
Size: 4073
BglI GCCn_nnn'nGGC
Cuts at: 662 671 662
Size: 9 4064
BglII A'GATC_T
Cuts at: 887 1588 3625 887
Size: 701 2037 1335
Fragments arranged by size:
2037 1335 701
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 226 484 557 634 1698 1791 2847 226
Size: 258 73 77 1064 93 1056 1452
Fragments arranged by size:
1452 1064 1056 258 93 77 73
BmgBI CAC'GTC
Cuts at: 510 510
Size: 4073
BmrI ACTGGGnnnn_n'
Cuts at: 725 1285 1366 1701 3845 725
Size: 560 81 335 2144 953
Fragments arranged by size:
2144 953 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 817
Size: 4073
Bpu10I CC'TnA_GC
Cuts at: 752 752
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2128 2620 2918 1249
Size: 879 492 298 2404
Fragments arranged by size:
2404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 428 602 837 1504 1752 2454 3950 428
Size: 174 235 667 248 702 1496 551
Fragments arranged by size:
1496 702 667 551 248 235 174
BsaWI w'CCGG_w
Cuts at: 902 1262 1783 2461 2735 2882 3293 902
Size: 360 521 678 274 147 411 1682
Fragments arranged by size:
1682 678 521 411 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 790 820 790
Size: 30 4043
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 1050 184
Size: 866 3207
BseYI C'CCAG_C
Cuts at: 243 257 281 449 483 521 558 660 1498
Size: 14 24 168 34 38 37 102 838
Cuts at: 1498 2256 2833 3329 243
Size: 758 577 496 987
Fragments arranged by size:
987 838 758 577 496 168 102 38
37 34 24 14
BsiEI CG_ry'CG
Cuts at: 104 306 445 1615 1661 2869 104
Size: 202 139 1170 46 1208 1308
Fragments arranged by size:
1308 1208 1170 202 139 46
BsiHKAI G_wGCw'C
Cuts at: 121 1042 1087 1865 2055 2847 121
Size: 921 45 778 190 792 1347
Fragments arranged by size:
1347 921 792 778 190 45
Bsp1286I G_dGCh'C
Cuts at: 121 152 226 314 353 484 557 634 658
Size: 31 74 88 39 131 73 77 24
Cuts at: 658 683 1012 1042 1087 1698 1791 1865 2055
Size: 25 329 30 45 611 93 74 190
Cuts at: 2055 2117 2847 121
Size: 62 730 1347
Fragments arranged by size:
1347 730 611 329 190 131 93 88
77 74 74 73 62 45 39 31
30 25 24
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 774 1123 1337 1639 2020 272
Size: 502 349 214 302 381 2325
Fragments arranged by size:
2325 502 381 349 302 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1985 1985
Size: 4073
BsrFI r'CCGG_y
Cuts at: 979 2071 2252 2461 979
Size: 1092 181 209 2591
Fragments arranged by size:
2591 1092 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 495 1450 495
Size: 955 3118
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstYI r'GATC_y
Cuts at: 1 707 887 1098 1565 1588 1923 2169 3181
Size: 706 180 211 467 23 335 246 1012
Cuts at: 3181 3267 3279 3625 1
Size: 86 12 346 449
Fragments arranged by size:
1012 706 467 449 346 335 246 211
180 86 23 12
BtgI C'CryG_G
Cuts at: 334 392 420 568 683 334
Size: 58 28 148 115 3724
Fragments arranged by size:
3724 148 115 58 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1316
Size: 4073
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 303 442 644 1658 1832 2223 2250 3595 303
Size: 139 202 1014 174 391 27 1345 781
Fragments arranged by size:
1345 1014 781 391 202 174 139 27
EagI C'GGCC_G
Cuts at: 303 442 1658 303
Size: 139 1216 2718
Fragments arranged by size:
2718 1216 139
EarI CTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 119 1085 119
Size: 966 3107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 817 1898 2330 3077 3345 688
Size: 129 1081 432 747 268 1416
Fragments arranged by size:
1416 1081 747 432 268 129
EcoO109I rG'GnC_Cy
Cuts at: 222 223 480 1212 222
Size: 1 257 732 3083
Fragments arranged by size:
3083 732 257 1
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 250 431 576 623 722 759 840 879 1507
Size: 181 145 47 99 37 81 39 628
Cuts at: 1507 1755 2777 3756 250
Size: 248 1022 979 567
Fragments arranged by size:
1022 979 628 567 248 181 145 99
81 47 39 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1915 1945 1947 1977 1915
Size: 30 2 30 4011
Fragments arranged by size:
4011 30 30 2
HincII GTy'rAC
Cuts at: 726 1112 726
Size: 386 3687
HpaI GTT'AAC
Cuts at: 726 726
Size: 4073
KasI G'GCGC_C
Cuts at: 427 836 1503 1751 427
Size: 409 667 248 2749
Fragments arranged by size:
2749 667 409 248
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 2744 2928 161
Size: 2583 184 1306
Fragments arranged by size:
2583 1306 184
MscI TGG'CCA
Cuts at: 1834 1834
Size: 4073
MslI CAynn'nnrTG
Cuts at: 112 946 2062 2189 112
Size: 834 1116 127 1996
Fragments arranged by size:
1996 1116 834 127
MspA1I CmG'CkG
Cuts at: 188 270 336 394 422 562 570 636 660
Size: 82 66 58 28 140 8 66 24
Cuts at: 660 1184 1498 1858 2871 3116 188
Size: 524 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 524 360 314 245 140 82
66 66 58 28 24 8
NaeI GCC'GGC
Cuts at: 981 2254 981
Size: 1273 2800
NarI GG'CG_CC
Cuts at: 428 837 1504 1752 428
Size: 409 667 248 2749
Fragments arranged by size:
2749 667 409 248
NgoMIV G'CCGG_C
Cuts at: 979 2252 979
Size: 1273 2800
NotI GC'GGCC_GC
Cuts at: 442 442
Size: 4073
NspI r_CATG'y
Cuts at: 502 1126 1626 2533 502
Size: 624 500 907 2042
Fragments arranged by size:
2042 907 624 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 139 171 790 822 3212 3244 139
Size: 32 619 32 2390 32 968
Fragments arranged by size:
2390 968 619 32 32 32
PsiI TTA'TAA
Cuts at: 737 737
Size: 4073
PspOMI G'GGCC_C
Cuts at: 222 480 222
Size: 258 3815
PstI C_TGCA'G
Cuts at: 275 518 542 1120 1805 275
Size: 243 24 578 685 2543
Fragments arranged by size:
2543 685 578 243 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 1498 1858 1498
Size: 360 3713
RsrII CG'GwC_CG
Cuts at: 338 2268 338
Size: 1930 2143
SacI G_AGCT'C
Cuts at: 121 1087 121
Size: 966 3107
SacII CC_GC'GG
Cuts at: 337 395 423 571 337
Size: 58 28 148 3839
Fragments arranged by size:
3839 148 58 28
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SfcI C'TryA_G
Cuts at: 33 271 514 538 1116 1801 2794 2985 33
Size: 238 243 24 578 685 993 191 1121
Fragments arranged by size:
1121 993 685 578 243 238 191 24
SfoI GGC'GCC
Cuts at: 429 838 1505 1753 429
Size: 409 667 248 2749
Fragments arranged by size:
2749 667 409 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 215 438 1095 215
Size: 223 657 3193
Fragments arranged by size:
3193 657 223
SmlI C'TyrA_G
Cuts at: 1228 2143 2635 2897 1228
Size: 915 492 262 2404
Fragments arranged by size:
2404 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 502 1126 1626 502
Size: 624 500 2949
Fragments arranged by size:
2949 624 500
SrfI GCCC'GGGC
Cuts at: 215 215
Size: 4073
StyI C'CwwG_G
Cuts at: 476 1207 1561 476
Size: 731 354 2988
Fragments arranged by size:
2988 731 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1656 1789 2248 2457 1656
Size: 133 459 209 3272
Fragments arranged by size:
3272 459 209 133
TatI w'GTAC_w
Cuts at: 3588 3588
Size: 4073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1038 1070 3590 3622 1038
Size: 32 2520 32 1489
Fragments arranged by size:
2520 1489 32 32
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 213 436 1093 213
Size: 223 657 3193
Fragments arranged by size:
3193 657 223
XmnI GAAnn'nnTTC
Cuts at: 805 805
Size: 4073
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PciI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NcoI
NdeI NheI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI ZraI