Restriction map for
pF1KB9672
(Circular) (Six-base) MAPSORT of: pF1KB9672.seq Check: 817 from: 1 to: 4385
pF1KB9672 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 670 724 731 1374 1423 670
Size: 54 7 643 49 3632
Fragments arranged by size:
3632 643 54 49 7
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 923 968 1198 2210 2642 3389 3657 421
Size: 502 45 230 1012 432 747 268 1149
Fragments arranged by size:
1149 1012 747 502 432 268 230 45
AflIII A'CryG_T
Cuts at: 312 1737 2841 3818 312
Size: 1425 1104 977 879
Fragments arranged by size:
1425 1104 977 879
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AleI CACnn'nnGTG
Cuts at: 1265 1265
Size: 4385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 679 1307 679
Size: 628 3757
AlwNI CAG_nnn'CTG
Cuts at: 984 1690 3257 984
Size: 706 1567 2112
Fragments arranged by size:
2112 1567 706
ApaLI G'TGCA_C
Cuts at: 462 3155 462
Size: 2693 1692
ApoI r'AATT_y
Cuts at: 195 573 602 755 1389 3738 3994 195
Size: 378 29 153 634 2349 256 586
Fragments arranged by size:
2349 634 586 378 256 153 29
AseI AT'TA_AT
Cuts at: 20 224 20
Size: 204 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1028 1405 1028
Size: 377 4008
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 1007 1401 1815 2063 2098 1007
Size: 394 414 248 35 3294
Fragments arranged by size:
3294 414 394 248 35
BanII G_rGCy'C
Cuts at: 1399 2429 1399
Size: 1030 3355
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 533 1195 533
Size: 662 3723
BciVI GTATCCnnnnn_n'
Cuts at: 772 2283 3044 4385 772
Size: 1511 761 1341 772
Fragments arranged by size:
1511 1341 772 761
BclI T'GATC_A
Cuts at: 872 1905 872
Size: 1033 3352
BfrBI ATG'CAT
Cuts at: 1074 1074
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 248 466 1018 2010 2103 3159 248
Size: 218 552 992 93 1056 1474
Fragments arranged by size:
1474 1056 992 552 218 93
BmrI ACTGGGnnnn_n'
Cuts at: 1597 1678 2013 4157 1597
Size: 81 335 2144 1825
Fragments arranged by size:
2144 1825 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 1006 398
Size: 608 3777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 2932 3230 1561
Size: 879 492 298 2716
Fragments arranged by size:
2716 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 146
Size: 30 4355
BseYI C'CCAG_C
Cuts at: 1810 2568 3145 3641 1810
Size: 758 577 496 2554
Fragments arranged by size:
2554 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 384 466 1399 2177 2367 3159 384
Size: 82 933 778 190 792 1610
Fragments arranged by size:
1610 933 792 778 190 82
BsmI GAATG_Cn'
Cuts at: 864 864
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 248 384 466 1018 1399 2010 2103 2177 2367
Size: 136 82 552 381 611 93 74 190
Cuts at: 2367 2429 3159 248
Size: 62 730 1474
Fragments arranged by size:
1474 730 611 552 381 190 136 93
82 74 62
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 663 2297 663
Size: 1634 2751
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstYI r'GATC_y
Cuts at: 1 1410 1877 1900 2235 2481 3493 3579 3591
Size: 1409 467 23 335 246 1012 86 12
Cuts at: 3591 3937 1
Size: 346 449
Fragments arranged by size:
1409 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1320 107
Size: 1213 3172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 1338 1628 1338
Size: 290 4095
DraI TTT'AAA
Cuts at: 744 1384 744
Size: 640 3745
DraIII CAC_nnn'GTG
Cuts at: 252 453 252
Size: 201 4184
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 1246 1970 2144 2535 2562 3907 1246
Size: 724 174 391 27 1345 1724
Fragments arranged by size:
1724 1345 724 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 641 834 2408 2618 641
Size: 193 1574 210 2408
Fragments arranged by size:
2408 1574 210 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 421 923 968 1006 1198 2210 2642 3389
Size: 23 502 45 38 192 1012 432 747
Cuts at: 3389 3657 398
Size: 268 1126
Fragments arranged by size:
1126 1012 747 502 432 268 192 45
38 23
EcoO109I rG'GnC_Cy
Cuts at: 1524 1524
Size: 4385
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 677 2404 677
Size: 1727 2658
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 1819 2067 3089 4068 1819
Size: 248 1022 979 2136
Fragments arranged by size:
2136 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 966 998 2227 2257 2259 2289 146
Size: 32 788 32 1229 30 2 30 2242
Fragments arranged by size:
2242 1229 788 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
HindIII A'AGCT_T
Cuts at: 470 470
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3240 3056
Size: 184 4201
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 277 1254 1265 2374 2501 277
Size: 977 11 1109 127 2161
Fragments arranged by size:
2161 1109 977 127 11
MspA1I CmG'CkG
Cuts at: 790 1496 1810 2170 3183 3428 790
Size: 706 314 360 1013 245 1747
Fragments arranged by size:
1747 1013 706 360 314 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 1320 107
Size: 1213 3172
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NsiI A_TGCA'T
Cuts at: 1076 1076
Size: 4385
NspI r_CATG'y
Cuts at: 316 846 1438 1938 2845 316
Size: 530 592 500 907 1856
Fragments arranged by size:
1856 907 592 530 500
PciI A'CATG_T
Cuts at: 312 2841 312
Size: 2529 1856
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PshAI GACnn'nnGTC
Cuts at: 533 533
Size: 4385
PspXI vC'TCGA_Gb
Cuts at: 1028 1028
Size: 4385
PstI C_TGCA'G
Cuts at: 1432 2117 1432
Size: 685 3700
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 790 1810 2170 790
Size: 1020 360 3005
Fragments arranged by size:
3005 1020 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 671 725 881 1428 2113 3106 3297 33
Size: 638 54 156 547 685 993 191 1121
Fragments arranged by size:
1121 993 685 638 547 191 156 54
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 1028 1540 2455 2947 3209 1028
Size: 512 915 492 262 2204
Fragments arranged by size:
2204 915 512 492 262
SpeI A'CTAG_T
Cuts at: 67 1377 67
Size: 1310 3075
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
StuI AGG'CCT
Cuts at: 1353 1353
Size: 4385
StyI C'CwwG_G
Cuts at: 107 1320 1519 1873 107
Size: 1213 199 354 2619
Fragments arranged by size:
2619 1213 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1968 2101 2560 2769 1968
Size: 133 459 209 3584
Fragments arranged by size:
3584 459 209 133
TatI w'GTAC_w
Cuts at: 784 1174 3900 784
Size: 390 2726 1269
Fragments arranged by size:
2726 1269 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1880 1880
Size: 4385
XhoI C'TCGA_G
Cuts at: 1028 1028
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PshAI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI