Restriction map for
pF1KB9669
(Circular) (Six-base) MAPSORT of: pF1KB9669.seq Check: 7787 from: 1 to: 4580
pF1KB9669 4580 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 253 1568 253
Size: 1315 3265
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 434 675 843 927 1095 1179 1347 1515
Size: 73 241 168 84 168 84 168 168
Cuts at: 1515 2405 2837 3584 3852 361
Size: 890 432 747 268 1089
Fragments arranged by size:
1089 890 747 432 268 241 168 168
168 168 84 84 73
AfeI AGC'GCT
Cuts at: 616 1144 616
Size: 528 4052
AflIII A'CryG_T
Cuts at: 758 833 917 1085 1337 1932 3036 4013 758
Size: 75 84 168 252 595 1104 977 1325
Fragments arranged by size:
1325 1104 977 595 252 168 84 75
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AlwNI CAG_nnn'CTG
Cuts at: 1885 3452 1885
Size: 1567 3013
ApaLI G'TGCA_C
Cuts at: 643 715 883 1303 1315 1471 3350 643
Size: 72 168 420 12 156 1879 1873
Fragments arranged by size:
1879 1873 420 168 156 72 12
ApoI r'AATT_y
Cuts at: 287 1584 3933 4189 287
Size: 1297 2349 256 678
Fragments arranged by size:
2349 1297 678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 925 1600 925
Size: 675 3905
BamHI G'GATC_C
Cuts at: 120 557 809 1397 1481 1605 120
Size: 437 252 588 84 124 3095
Fragments arranged by size:
3095 588 437 252 124 84
BanI G'GyrC_C
Cuts at: 193 1596 2010 2258 2293 193
Size: 1403 414 248 35 2480
Fragments arranged by size:
2480 1403 414 248 35
BanII G_rGCy'C
Cuts at: 319 1039 1123 1594 2624 319
Size: 720 84 471 1030 2275
Fragments arranged by size:
2275 1030 720 471 84
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BbsI GAAGACnn'nnnn_
Cuts at: 149 787 1531 149
Size: 638 744 3198
Fragments arranged by size:
3198 744 638
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 2100 2100
Size: 4580
BglI GCCn_nnn'nGGC
Cuts at: 246 246
Size: 4580
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 1692 1692
Size: 4580
Bme1580I G_kGCm'C
Cuts at: 647 719 887 925 1307 1319 1475 2205 2298
Size: 72 168 38 382 12 156 730 93
Cuts at: 2298 3354 647
Size: 1056 1873
Fragments arranged by size:
1873 1056 730 382 168 156 93 72
38 12
BmgBI CAC'GTC
Cuts at: 759 759
Size: 4580
BmrI ACTGGGnnnn_n'
Cuts at: 1792 1873 2208 4352 1792
Size: 81 335 2144 2020
Fragments arranged by size:
2144 2020 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 426
Size: 4580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4284 4284
Size: 4580
BsaAI yAC'GTr
Cuts at: 918 1086 1338 1933 918
Size: 168 252 595 3565
Fragments arranged by size:
3565 595 252 168
BsaBI GATnn'nnATC
Cuts at: 6 2094 4131 6
Size: 2088 2037 455
Fragments arranged by size:
2088 2037 455
BsaHI Gr'CG_yC
Cuts at: 2011 2259 2961 4457 2011
Size: 248 702 1496 2134
Fragments arranged by size:
2134 1496 702 248
BsaWI w'CCGG_w
Cuts at: 776 1769 2290 2968 3242 3389 3800 776
Size: 993 521 678 274 147 411 1556
Fragments arranged by size:
1556 993 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 898 928 982 1012 1021 1051 136
Size: 30 732 30 54 30 9 30 3665
Fragments arranged by size:
3665 732 54 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 1029 1197 1449 496
Size: 533 168 252 3627
Fragments arranged by size:
3627 533 252 168
BseYI C'CCAG_C
Cuts at: 797 2005 2763 3340 3836 797
Size: 1208 758 577 496 1541
Fragments arranged by size:
1541 1208 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1036 1194 287
Size: 749 158 3673
Fragments arranged by size:
3673 749 158
BsiEI CG_ry'CG
Cuts at: 104 2122 2168 3376 104
Size: 2018 46 1208 1308
Fragments arranged by size:
2018 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 647 719 887 971 1039 1123 1307 1319 1391
Size: 72 168 84 68 84 184 12 72
Cuts at: 1391 1475 1594 2372 2562 3354 647
Size: 84 119 778 190 792 1873
Fragments arranged by size:
1873 792 778 190 184 168 119 84
84 84 72 72 68 12
BsiWI C'GTAC_G
Cuts at: 914 998 914
Size: 84 4496
Bsp1286I G_dGCh'C
Cuts at: 319 647 719 887 925 971 1039 1123 1307
Size: 328 72 168 38 46 68 84 184
Cuts at: 1307 1319 1391 1475 1594 2205 2298 2372 2562
Size: 12 72 84 119 611 93 74 190
Cuts at: 2562 2624 3354 319
Size: 62 730 1545
Fragments arranged by size:
1545 730 611 328 190 184 168 119
93 84 84 74 72 72 68 62
46 38 12
BspEI T'CCGG_A
Cuts at: 776 3800 776
Size: 3024 1556
BspHI T'CATG_A
Cuts at: 333 3756 333
Size: 3423 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 253 1568 1630 1844 2146 2527 253
Size: 1315 62 214 302 381 2306
Fragments arranged by size:
2306 1315 381 302 214 62
BsrBI CCG'CTC
Cuts at: 50 807 2872 2926 4410 4575 50
Size: 757 2065 54 1484 165 55
Fragments arranged by size:
2065 1484 757 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2492 2492
Size: 4580
BsrFI r'CCGG_y
Cuts at: 1069 1237 1405 2578 2759 2968 1069
Size: 168 168 1173 181 209 2681
Fragments arranged by size:
2681 1173 209 181 168 168
BssSI C'ACGA_G
Cuts at: 183 802 2851 3209 183
Size: 619 2049 358 1554
Fragments arranged by size:
2049 1554 619 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1013 1181 1957 1013
Size: 168 776 3636
Fragments arranged by size:
3636 776 168
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstXI CCAn_nnnn'nTGG
Cuts at: 236 236
Size: 4580
BstYI r'GATC_y
Cuts at: 1 120 557 809 1397 1481 1605 2072 2095
Size: 119 437 252 588 84 124 467 23
Cuts at: 2095 2430 2676 3688 3774 3786 4132 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 588 467 449 437 346 335 252
246 124 119 86 84 23 12
BtgI C'CryG_G
Cuts at: 170 250 379 170
Size: 80 129 4371
Fragments arranged by size:
4371 129 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2530 2711 2874 2530
Size: 181 163 4236
Fragments arranged by size:
4236 181 163
BtsI GCAGTG_nn'
Cuts at: 1823 1823
Size: 4580
DraI TTT'AAA
Cuts at: 1579 1579
Size: 4580
DrdI GACnn_nn'nnGTC
Cuts at: 2286 3144 2286
Size: 858 3722
EaeI y'GGCC_r
Cuts at: 238 1428 2165 2339 2730 2757 4102 238
Size: 1190 737 174 391 27 1345 716
Fragments arranged by size:
1345 1190 737 716 391 174 27
EagI C'GGCC_G
Cuts at: 2165 2165
Size: 4580
EarI CTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1121 1592 1037
Size: 84 471 4025
Fragments arranged by size:
4025 471 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 426 434 675 843 927 1095 1179 1347
Size: 65 8 241 168 84 168 84 168
Cuts at: 1347 1515 2405 2837 3584 3852 361
Size: 168 890 432 747 268 1089
Fragments arranged by size:
1089 890 747 432 268 241 168 168
168 168 84 84 65 8
EcoO109I rG'GnC_Cy
Cuts at: 178 202 407 1719 178
Size: 24 205 1312 3039
Fragments arranged by size:
3039 1312 205 24
EcoRI G'AATT_C
Cuts at: 287 1584 287
Size: 1297 3283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1293 2599 1293
Size: 1306 3274
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 618 1146 1290 1376 2014 2262 3284 4263 618
Size: 528 144 86 638 248 1022 979 935
Fragments arranged by size:
1022 979 935 638 528 248 144 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 604 636 774 806 2422 2452 2454
Size: 32 436 32 138 32 1616 30 2
Cuts at: 2454 2484 136
Size: 30 2232
Fragments arranged by size:
2232 1616 436 138 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 833 4013 833
Size: 3180 1400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 98 3251 3435 98
Size: 3153 184 1243
Fragments arranged by size:
3153 1243 184
MscI TGG'CCA
Cuts at: 240 2341 240
Size: 2101 2479
MslI CAynn'nnrTG
Cuts at: 1156 2569 2696 1156
Size: 1413 127 3040
Fragments arranged by size:
3040 1413 127
MspA1I CmG'CkG
Cuts at: 236 252 639 1395 1479 1691 2005 2365 3378
Size: 16 387 756 84 212 314 360 1013
Cuts at: 3378 3623 236
Size: 245 1193
Fragments arranged by size:
1193 1013 756 387 360 314 245 212
84 16
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 4580
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 4580
NspI r_CATG'y
Cuts at: 332 873 957 1633 2133 3040 332
Size: 541 84 676 500 907 1872
Fragments arranged by size:
1872 907 676 541 500 84
PasI CC'CwG_GG
Cuts at: 294 294
Size: 4580
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PpuMI rG'GwC_Cy
Cuts at: 178 202 178
Size: 24 4556
PstI C_TGCA'G
Cuts at: 505 1627 2312 505
Size: 1122 685 2773
Fragments arranged by size:
2773 1122 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 236 2005 2365 236
Size: 1769 360 2451
Fragments arranged by size:
2451 1769 360
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 1039 1123 1594 1039
Size: 84 471 4025
Fragments arranged by size:
4025 471 84
SacII CC_GC'GG
Cuts at: 253 253
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SanDI GG'GwC_CC
Cuts at: 178 178
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 501 1623 2308 3301 3492 33
Size: 468 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 468 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 246 246
Size: 4580
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SgrAI Cr'CCGG_yG
Cuts at: 1069 1237 1405 1069
Size: 168 168 4244
Fragments arranged by size:
4244 168 168
SmaI CCC'GGG
Cuts at: 1602 1602
Size: 4580
SmlI C'TyrA_G
Cuts at: 1735 2650 3142 3404 1735
Size: 915 492 262 2911
Fragments arranged by size:
2911 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
StuI AGG'CCT
Cuts at: 520 688 772 856 1024 1108 1192 1360 520
Size: 168 84 84 168 84 84 168 3740
Fragments arranged by size:
3740 168 168 168 84 84 84 84
StyI C'CwwG_G
Cuts at: 357 1714 2068 357
Size: 1357 354 2869
Fragments arranged by size:
2869 1357 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 811 979 2163 2296 2755 2964 811
Size: 168 1184 133 459 209 2427
Fragments arranged by size:
2427 1184 459 209 168 133
TatI w'GTAC_w
Cuts at: 4095 4095
Size: 4580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 4548
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2075 114
Size: 1961 2619
XmaI C'CCGG_G
Cuts at: 1600 1600
Size: 4580
XmnI GAAnn'nnTTC
Cuts at: 604 1165 1276 1444 1528 604
Size: 561 111 168 84 3656
Fragments arranged by size:
3656 561 168 111 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SexAI
SnaBI SrfI SspI SwaI XhoI ZraI