Restriction map for
pF1KB9667
(Circular) (Six-base) MAPSORT of: pF1KB9667.seq Check: 8758 from: 1 to: 4043
pF1KB9667 4043 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 927 3883 927
Size: 2956 1087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1868 2300 3047 3315 142
Size: 1726 432 747 268 870
Fragments arranged by size:
1726 870 747 432 268
AfeI AGC'GCT
Cuts at: 871 871
Size: 4043
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AleI CACnn'nnGTG
Cuts at: 761 761
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 120 1348 2915 120
Size: 1228 1567 1248
Fragments arranged by size:
1567 1248 1228
ApaLI G'TGCA_C
Cuts at: 846 2813 846
Size: 1967 2076
ApoI r'AATT_y
Cuts at: 522 616 1047 3396 3652 522
Size: 94 431 2349 256 913
Fragments arranged by size:
2349 913 431 256 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 749 1063 749
Size: 314 3729
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 1059 1473 1721 1756 1059
Size: 414 248 35 3346
Fragments arranged by size:
3346 414 248 35
BanII G_rGCy'C
Cuts at: 171 796 1057 2087 171
Size: 625 261 1030 2127
Fragments arranged by size:
2127 1030 625 261
BbeI G_GCGC'C
Cuts at: 1477 1725 1477
Size: 248 3795
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 182 1155 182
Size: 973 3070
Bme1580I G_kGCm'C
Cuts at: 850 1668 1761 2817 850
Size: 818 93 1056 2076
Fragments arranged by size:
2076 1056 818 93
BmgBI CAC'GTC
Cuts at: 759 759
Size: 4043
BmrI ACTGGGnnnn_n'
Cuts at: 167 1255 1336 1671 3815 167
Size: 1088 81 335 2144 395
Fragments arranged by size:
2144 1088 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 848 590
Size: 258 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1219 2098 2590 2888 550
Size: 669 879 492 298 1705
Fragments arranged by size:
1705 879 669 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 746 3747 746
Size: 3001 1042
BsaAI yAC'GTr
Cuts at: 903 1396 903
Size: 493 3550
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 1474 1722 2424 3920 1474
Size: 248 702 1496 1597
Fragments arranged by size:
1597 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 724
Size: 4043
BseYI C'CCAG_C
Cuts at: 1468 2226 2803 3299 1468
Size: 758 577 496 2212
Fragments arranged by size:
2212 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 850 1057 1835 2025 2817 796
Size: 54 207 778 190 792 2022
Fragments arranged by size:
2022 792 778 207 190 54
BsiWI C'GTAC_G
Cuts at: 895 895
Size: 4043
BsmI GAATG_Cn'
Cuts at: 334 334
Size: 4043
Bsp1286I G_dGCh'C
Cuts at: 171 796 850 1057 1668 1761 1835 2025 2087
Size: 625 54 207 611 93 74 190 62
Cuts at: 2087 2817 171
Size: 730 1397
Fragments arranged by size:
1397 730 625 611 207 190 93 74
62 54
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 1093 1307 1609 1990 221
Size: 872 214 302 381 2274
Fragments arranged by size:
2274 872 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 2041 2222 2431 2041
Size: 181 209 3653
Fragments arranged by size:
3653 209 181
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 619 1420 619
Size: 801 3242
BstBI TT'CG_AA
Cuts at: 440 2404 440
Size: 1964 2079
BstYI r'GATC_y
Cuts at: 1 597 1068 1535 1558 1893 2139 3151 3237
Size: 596 471 467 23 335 246 1012 86
Cuts at: 3237 3249 3595 1
Size: 12 346 449
Fragments arranged by size:
1012 596 471 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 287 287
Size: 4043
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 1286 1286
Size: 4043
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DrdI GACnn_nn'nnGTC
Cuts at: 1749 2607 1749
Size: 858 3185
EaeI y'GGCC_r
Cuts at: 1628 1802 2193 2220 3565 1628
Size: 174 391 27 1345 2106
Fragments arranged by size:
2106 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 159 362 2066 2276 159
Size: 203 1704 210 1926
Fragments arranged by size:
1926 1704 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 235 554 2251 2561 2707 3853 235
Size: 319 1697 310 146 1146 425
Fragments arranged by size:
1697 1146 425 319 310 146
EcoICRI GAG'CTC
Cuts at: 794 1055 794
Size: 261 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 590 848 1868 2300 3047 3315 142
Size: 448 258 1020 432 747 268 870
Fragments arranged by size:
1020 870 747 448 432 268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 367 367
Size: 4043
EcoO109I rG'GnC_Cy
Cuts at: 1182 1182
Size: 4043
EcoRI G'AATT_C
Cuts at: 522 1047 522
Size: 525 3518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 4043
FspI TGC'GCA
Cuts at: 907 1824 907
Size: 917 3126
FspAI rTGC'GCAy
Cuts at: 907 907
Size: 4043
HaeII r_GCGC'y
Cuts at: 873 1477 1725 2747 3726 873
Size: 604 248 1022 979 1190
Fragments arranged by size:
1190 1022 979 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 714 743 746 775 1885 1915 1917
Size: 32 387 29 3 29 1110 30 2
Cuts at: 1917 1947 295
Size: 30 2391
Fragments arranged by size:
2391 1110 387 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 1082 1082
Size: 4043
HindIII A'AGCT_T
Cuts at: 559 559
Size: 4043
KasI G'GCGC_C
Cuts at: 1473 1721 1473
Size: 248 3795
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 495 2714 2898 495
Size: 2219 184 1640
Fragments arranged by size:
2219 1640 184
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 761 2032 2159 761
Size: 1271 127 2645
Fragments arranged by size:
2645 1271 127
MspA1I CmG'CkG
Cuts at: 1154 1468 1828 2841 3086 1154
Size: 314 360 1013 245 2111
Fragments arranged by size:
2111 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 4043
NarI GG'CG_CC
Cuts at: 1474 1722 1474
Size: 248 3795
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 4043
NspI r_CATG'y
Cuts at: 1006 1096 1596 2503 1006
Size: 90 500 907 2546
Fragments arranged by size:
2546 907 500 90
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PfoI T'CCnGG_A
Cuts at: 362 362
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PmlI CAC'GTG
Cuts at: 903 903
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PstI C_TGCA'G
Cuts at: 218 1090 1775 218
Size: 872 685 2486
Fragments arranged by size:
2486 872 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 1468 1828 1468
Size: 360 3683
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 796 1057 796
Size: 261 3782
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 159 2066 2276 159
Size: 1907 210 1926
Fragments arranged by size:
1926 1907 210
SbfI CC_TGCA'GG
Cuts at: 218 1090 218
Size: 872 3171
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 214 1086 1771 2764 2955 33
Size: 181 872 685 993 191 1121
Fragments arranged by size:
1121 993 872 685 191 181
SfoI GGC'GCC
Cuts at: 1475 1723 1475
Size: 248 3795
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 1065 1065
Size: 4043
SmlI C'TyrA_G
Cuts at: 529 1198 2113 2605 2867 529
Size: 669 915 492 262 1705
Fragments arranged by size:
1705 915 669 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1006 1096 1596 1006
Size: 90 500 3453
Fragments arranged by size:
3453 500 90
StuI AGG'CCT
Cuts at: 155 155
Size: 4043
StyI C'CwwG_G
Cuts at: 389 1177 1531 389
Size: 788 354 2901
Fragments arranged by size:
2901 788 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 1626 1759 2218 2427 148
Size: 1478 133 459 209 1764
Fragments arranged by size:
1764 1478 459 209 133
TatI w'GTAC_w
Cuts at: 542 3558 542
Size: 3016 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4011
Tth111I GACn'n_nGTC
Cuts at: 757 1840 757
Size: 1083 2960
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1538 1538
Size: 4043
XmaI C'CCGG_G
Cuts at: 1063 1063
Size: 4043
XmnI GAAnn'nnTTC
Cuts at: 781 928 781
Size: 147 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI