Restriction map for
pF1KB9665
(Circular) (Six-base) MAPSORT of: pF1KB9665.seq Check: 9276 from: 1 to: 4382
pF1KB9665 4382 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 362 782 1119 2207 2639 3386 3654 110
Size: 252 420 337 1088 432 747 268 838
Fragments arranged by size:
1088 838 747 432 420 337 268 252
AflIII A'CryG_T
Cuts at: 1141 1734 2838 3815 1141
Size: 593 1104 977 1708
Fragments arranged by size:
1708 1104 977 593
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AleI CACnn'nnGTG
Cuts at: 757 1094 757
Size: 337 4045
AlwNI CAG_nnn'CTG
Cuts at: 1687 3254 1687
Size: 1567 2815
ApaLI G'TGCA_C
Cuts at: 861 3152 861
Size: 2291 2091
ApoI r'AATT_y
Cuts at: 218 412 496 832 1386 3735 3991 218
Size: 194 84 336 554 2349 256 609
Fragments arranged by size:
2349 609 554 336 256 194 84
AseI AT'TA_AT
Cuts at: 20 400 20
Size: 380 4002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BamHI G'GATC_C
Cuts at: 244 1407 244
Size: 1163 3219
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 138 306 558 1396 2426 138
Size: 168 252 838 1030 2094
Fragments arranged by size:
2094 1030 838 252 168
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 1274 1274
Size: 4382
BbvCI CC'TCA_GC
Cuts at: 1178 1178
Size: 4382
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 359 1902 359
Size: 1543 2839
BfrBI ATG'CAT
Cuts at: 606 690 1120 606
Size: 84 430 3868
Fragments arranged by size:
3868 430 84
BglII A'GATC_T
Cuts at: 140 392 476 644 890 1897 3934 140
Size: 252 84 168 246 1007 2037 588
Fragments arranged by size:
2037 1007 588 252 246 168 84
BlpI GC'TnA_GC
Cuts at: 978 1494 978
Size: 516 3866
Bme1580I G_kGCm'C
Cuts at: 865 2007 2100 3156 865
Size: 1142 93 1056 2091
Fragments arranged by size:
2091 1142 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 178 935 1019 1594 1675 2010 4154 178
Size: 757 84 575 81 335 2144 406
Fragments arranged by size:
2144 757 575 406 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 358 442 610 694 862 274
Size: 84 84 168 84 168 3794
Fragments arranged by size:
3794 168 168 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 1178 1178
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1195 1195
Size: 4382
BseYI C'CCAG_C
Cuts at: 1807 2565 3142 3638 1807
Size: 758 577 496 2551
Fragments arranged by size:
2551 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 975 1227 891
Size: 84 252 4046
Fragments arranged by size:
4046 252 84
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 138 306 558 865 979 1396 2174 2364 3156
Size: 168 252 307 114 417 778 190 792
Cuts at: 3156 138
Size: 1364
Fragments arranged by size:
1364 792 778 417 307 252 190 168
114
BsmI GAATG_Cn'
Cuts at: 1368 1368
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 138 306 558 865 979 1396 2007 2100 2174
Size: 168 252 307 114 417 611 93 74
Cuts at: 2174 2364 2426 3156 138
Size: 190 62 730 1364
Fragments arranged by size:
1364 730 611 417 307 252 190 168
114 93 74 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 1245 3558 1245
Size: 2313 2069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 773 2294 773
Size: 1521 2861
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BsrGI T'GTAC_A
Cuts at: 1309 1309
Size: 4382
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 869 953 1759 869
Size: 84 806 3492
Fragments arranged by size:
3492 806 84
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4382
BstYI r'GATC_y
Cuts at: 1 140 244 392 476 644 890 1407 1874
Size: 139 104 148 84 168 246 517 467
Cuts at: 1874 1897 2232 2478 3490 3576 3588 3934 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 517 467 449 346 335 246 246
168 148 139 104 86 84 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 954 1625 954
Size: 671 3711
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 869 869
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 1967 2141 2532 2559 3904 1967
Size: 174 391 27 1345 2445
Fragments arranged by size:
2445 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 136 304 556 1394 136
Size: 168 252 838 3124
Fragments arranged by size:
3124 838 252 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 274 358 362 442 610 694 782 862
Size: 164 84 4 80 168 84 88 80
Cuts at: 862 1119 2207 2639 3386 3654 110
Size: 257 1088 432 747 268 838
Fragments arranged by size:
1088 838 747 432 268 257 168 164
88 84 84 80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 380 380
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 1521 1521
Size: 4382
EcoRI G'AATT_C
Cuts at: 218 412 496 832 1386 218
Size: 194 84 336 554 3214
Fragments arranged by size:
3214 554 336 194 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 2224 2254 2256 2286 125
Size: 32 2067 30 2 30 2221
Fragments arranged by size:
2221 2067 32 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
HindIII A'AGCT_T
Cuts at: 289 626 1047 289
Size: 337 421 3624
Fragments arranged by size:
3624 421 337
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 583 3053 3237 583
Size: 2470 184 1728
Fragments arranged by size:
2470 1728 184
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 112 185 505 689 757 942 1094 2371 2498
Size: 73 320 184 68 185 152 1277 127
Cuts at: 2498 112
Size: 1996
Fragments arranged by size:
1996 1277 320 185 184 152 127 73
68
MspA1I CmG'CkG
Cuts at: 1493 1807 2167 3180 3425 1493
Size: 314 360 1013 245 2450
Fragments arranged by size:
2450 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NdeI CA'TA_TG
Cuts at: 686 686
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NsiI A_TGCA'T
Cuts at: 608 692 1122 608
Size: 84 430 3868
Fragments arranged by size:
3868 430 84
NspI r_CATG'y
Cuts at: 610 694 1124 1145 1435 1935 2842 610
Size: 84 430 21 290 500 907 2150
Fragments arranged by size:
2150 907 500 430 290 84 21
PciI A'CATG_T
Cuts at: 1141 2838 1141
Size: 1697 2685
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 1807 2167 1807
Size: 360 4022
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 138 306 558 1396 138
Size: 168 252 838 3124
Fragments arranged by size:
3124 838 252 168
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 1537 2452 2944 3206 1537
Size: 915 492 262 2713
Fragments arranged by size:
2713 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 1516 1870 1516
Size: 354 4028
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1965 2098 2557 2766 1965
Size: 133 459 209 3581
Fragments arranged by size:
3581 459 209 133
TatI w'GTAC_w
Cuts at: 618 749 1144 1309 3897 618
Size: 131 395 165 2588 1103
Fragments arranged by size:
2588 1103 395 165 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 1877
Size: 4382
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
XmnI GAAnn'nnTTC
Cuts at: 123 795 964 1132 123
Size: 672 169 168 3373
Fragments arranged by size:
3373 672 169 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI