Restriction map for
pF1KB9664
(Circular) (Six-base) MAPSORT of: pF1KB9664.seq Check: 4562 from: 1 to: 4487
pF1KB9664 4487 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 390 390
Size: 4487
AccI GT'mk_AC
Cuts at: 1525 1525
Size: 4487
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 738 1074 1158 1410 2312 2744 3491 3759
Size: 415 336 84 252 902 432 747 268
Cuts at: 3759 323
Size: 1051
Fragments arranged by size:
1051 902 747 432 415 336 268 252
84
AfeI AGC'GCT
Cuts at: 1099 1099
Size: 4487
AflIII A'CryG_T
Cuts at: 1839 2943 3920 1839
Size: 1104 977 2406
Fragments arranged by size:
2406 1104 977
AgeI A'CCGG_T
Cuts at: 1202 2875 1202
Size: 1673 2814
AlwNI CAG_nnn'CTG
Cuts at: 1792 3359 1792
Size: 1567 2920
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 1491 3840 4096 1491
Size: 2349 256 1882
Fragments arranged by size:
2349 1882 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 849 1507 849
Size: 658 3829
BamHI G'GATC_C
Cuts at: 549 1512 549
Size: 963 3524
BanI G'GyrC_C
Cuts at: 1503 1917 2165 2200 1503
Size: 414 248 35 3790
Fragments arranged by size:
3790 414 248 35
BanII G_rGCy'C
Cuts at: 850 934 1018 1186 1270 1438 1501 2531 850
Size: 84 84 168 84 168 63 1030 2806
Fragments arranged by size:
2806 1030 168 168 84 84 84 63
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BglI GCCn_nnn'nGGC
Cuts at: 527 527
Size: 4487
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 1599 1599
Size: 4487
Bme1580I G_kGCm'C
Cuts at: 2112 2205 3261 2112
Size: 93 1056 3338
Fragments arranged by size:
3338 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1699 1780 2115 4259 1699
Size: 81 335 2144 1927
Fragments arranged by size:
2144 1927 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1322 1406 1070
Size: 252 84 4151
Fragments arranged by size:
4151 252 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 918 1663 2542 3034 3332 297
Size: 621 745 879 492 298 1452
Fragments arranged by size:
1452 879 745 621 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 782 4191 782
Size: 3409 1078
BsaAI yAC'GTr
Cuts at: 1840 1840
Size: 4487
BsaBI GATnn'nnATC
Cuts at: 6 588 689 2001 4038 6
Size: 582 101 1312 2037 455
Fragments arranged by size:
2037 1312 582 455 101
BsaHI Gr'CG_yC
Cuts at: 387 1918 2166 2868 4364 387
Size: 1531 248 702 1496 510
Fragments arranged by size:
1531 1496 702 510 248
BsaWI w'CCGG_w
Cuts at: 1202 1676 2197 2875 3149 3296 3707 1202
Size: 474 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 474 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 837 867 921 951 837
Size: 30 54 30 4373
Fragments arranged by size:
4373 54 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 1087 1351 646
Size: 441 264 3782
Fragments arranged by size:
3782 441 264
BseYI C'CCAG_C
Cuts at: 1912 2670 3247 3743 1912
Size: 758 577 496 2656
Fragments arranged by size:
2656 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1093 1425 1009
Size: 84 332 4071
Fragments arranged by size:
4071 332 84
BsiEI CG_ry'CG
Cuts at: 104 2029 2075 3283 104
Size: 1925 46 1208 1308
Fragments arranged by size:
1925 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 850 934 1018 1186 1270 1438 1501 2279 2469
Size: 84 84 168 84 168 63 778 190
Cuts at: 2469 3261 850
Size: 792 2076
Fragments arranged by size:
2076 792 778 190 168 168 84 84
84 63
BsmBI CGTCTCn'nnnn_
Cuts at: 394 394
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 850 934 1018 1186 1270 1438 1501 2112 2205
Size: 84 84 168 84 168 63 611 93
Cuts at: 2205 2279 2469 2531 3261 850
Size: 74 190 62 730 2076
Fragments arranged by size:
2076 730 611 190 168 168 93 84
84 84 74 63 62
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1115 1537 1751 2053 2434 1115
Size: 422 214 302 381 3168
Fragments arranged by size:
3168 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 829 2399 829
Size: 1570 2917
BsrFI r'CCGG_y
Cuts at: 1202 2485 2666 2875 1202
Size: 1283 181 209 2814
Fragments arranged by size:
2814 1283 209 181
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 272 2848 272
Size: 2576 1911
BstYI r'GATC_y
Cuts at: 1 549 1272 1473 1512 1979 2002 2337 2583
Size: 548 723 201 39 467 23 335 246
Cuts at: 2583 3595 3681 3693 4039 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 723 548 467 449 346 335 246
201 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 365 365
Size: 4487
BtgI C'CryG_G
Cuts at: 107 107
Size: 4487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 710 1730 710
Size: 1020 3467
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 110 528 2072 2246 2637 2664 4009 110
Size: 418 1544 174 391 27 1345 588
Fragments arranged by size:
1544 1345 588 418 391 174 27
EagI C'GGCC_G
Cuts at: 2072 2072
Size: 4487
EarI CTCTTCn'nnn_
Cuts at: 260 342 2510 2720 260
Size: 82 2168 210 2027
Fragments arranged by size:
2168 2027 210 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2695 3005 3151 4297 2695
Size: 310 146 1146 2885
Fragments arranged by size:
2885 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 932 1016 1184 1268 1436 1499 848
Size: 84 84 168 84 168 63 3836
Fragments arranged by size:
3836 168 168 84 84 84 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 738 1070 1074 1158 1322 1406 1410 2312
Size: 415 332 4 84 164 84 4 902
Cuts at: 2312 2744 3491 3759 323
Size: 432 747 268 1051
Fragments arranged by size:
1051 902 747 432 415 332 268 164
84 84 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1142 1626 1142
Size: 484 4003
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 1101 1921 2169 3191 4170 1101
Size: 820 248 1022 979 1418
Fragments arranged by size:
1418 1022 979 820 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 837 869 921 953 1077 1109 1257 1289 2329
Size: 32 52 32 124 32 148 32 1040
Cuts at: 2329 2359 2361 2391 837
Size: 30 2 30 2933
Fragments arranged by size:
2933 1040 148 124 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 199 1526 199
Size: 1327 3160
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1463 3158 3342 1463
Size: 1695 184 2608
Fragments arranged by size:
2608 1695 184
MscI TGG'CCA
Cuts at: 112 530 2248 112
Size: 418 1718 2351
Fragments arranged by size:
2351 1718 418
MslI CAynn'nnrTG
Cuts at: 695 881 2476 2603 695
Size: 186 1595 127 2579
Fragments arranged by size:
2579 1595 186 127
MspA1I CmG'CkG
Cuts at: 1458 1598 1912 2272 3285 3530 1458
Size: 140 314 360 1013 245 2415
Fragments arranged by size:
2415 1013 360 314 245 140
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 4487
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 107
Size: 4487
NdeI CA'TA_TG
Cuts at: 696 696
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 4487
NspI r_CATG'y
Cuts at: 1540 2040 2947 1540
Size: 500 907 3080
Fragments arranged by size:
3080 907 500
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PsiI TTA'TAA
Cuts at: 896 980 896
Size: 84 4403
PspXI vC'TCGA_Gb
Cuts at: 849 849
Size: 4487
PstI C_TGCA'G
Cuts at: 717 1534 2219 717
Size: 817 685 2985
Fragments arranged by size:
2985 817 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 1912 2272 1912
Size: 360 4127
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 850 934 1018 1186 1270 1438 1501 850
Size: 84 84 168 84 168 63 3836
Fragments arranged by size:
3836 168 168 84 84 84 63
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 2510 2720 2510
Size: 210 4277
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SfcI C'TryA_G
Cuts at: 33 713 1530 2215 3208 3399 33
Size: 680 817 685 993 191 1121
Fragments arranged by size:
1121 993 817 685 680 191
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SgrAI Cr'CCGG_yG
Cuts at: 1202 1202
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 312 849 933 1642 2557 3049 3311 312
Size: 537 84 709 915 492 262 1488
Fragments arranged by size:
1488 915 709 537 492 262 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
StyI C'CwwG_G
Cuts at: 107 1621 1975 107
Size: 1514 354 2619
Fragments arranged by size:
2619 1514 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2203 2662 2871 2070
Size: 133 459 209 3686
Fragments arranged by size:
3686 459 209 133
TatI w'GTAC_w
Cuts at: 115 1125 1377 4002 115
Size: 1010 252 2625 600
Fragments arranged by size:
2625 1010 600 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4004 4036 4004
Size: 32 4455
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 655 1982 655
Size: 1327 3160
XhoI C'TCGA_G
Cuts at: 849 849
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
XmnI GAAnn'nnTTC
Cuts at: 835 919 1171 1255 1423 835
Size: 84 252 84 168 3899
Fragments arranged by size:
3899 252 168 84 84
ZraI GAC'GTC
Cuts at: 388 388
Size: 4487
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI