Restriction map for
pF1KB9662
(Circular) (Six-base) MAPSORT of: pF1KB9662.seq Check: 8864 from: 1 to: 4124
pF1KB9662 4124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 572 1140 572
Size: 568 3556
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1033 1949 2381 3128 3396 598
Size: 435 916 432 747 268 1326
Fragments arranged by size:
1326 916 747 435 432 268
AflIII A'CryG_T
Cuts at: 590 1476 2580 3557 590
Size: 886 1104 977 1157
Fragments arranged by size:
1157 1104 977 886
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaI G_GGCC'C
Cuts at: 634 634
Size: 4124
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 742 1128 3477 3733 742
Size: 386 2349 256 1133
Fragments arranged by size:
2349 1133 386 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 229 305 612 772 1144 229
Size: 76 307 160 372 3209
Fragments arranged by size:
3209 372 307 160 76
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 294 363 572 1140 1554 1802 1837 294
Size: 69 209 568 414 248 35 2581
Fragments arranged by size:
2581 568 414 248 209 69 35
BanII G_rGCy'C
Cuts at: 634 1138 2168 634
Size: 504 1030 2590
Fragments arranged by size:
2590 1030 504
BbeI G_GCGC'C
Cuts at: 367 1558 1806 367
Size: 1191 248 2685
Fragments arranged by size:
2685 1191 248
BbsI GAAGACnn'nnnn_
Cuts at: 1094 1094
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglI GCCn_nnn'nGGC
Cuts at: 417 417
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 278 1236 278
Size: 958 3166
Bme1580I G_kGCm'C
Cuts at: 634 1749 1842 2898 634
Size: 1115 93 1056 1860
Fragments arranged by size:
1860 1115 1056 93
BmgBI CAC'GTC
Cuts at: 381 593 381
Size: 212 3912
BmrI ACTGGGnnnn_n'
Cuts at: 898 1336 1417 1752 3896 898
Size: 438 81 335 2144 1126
Fragments arranged by size:
2144 1126 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 970 110
Size: 860 3264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1300 2179 2671 2969 475
Size: 825 879 492 298 1630
Fragments arranged by size:
1630 879 825 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 640 1477 640
Size: 837 3287
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 364 1555 1803 2505 4001 364
Size: 1191 248 702 1496 487
Fragments arranged by size:
1496 1191 702 487 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 597 600 370
Size: 227 3 3894
Fragments arranged by size:
3894 227 3
BseYI C'CCAG_C
Cuts at: 671 1549 2307 2884 3380 671
Size: 878 758 577 496 1415
Fragments arranged by size:
1415 878 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 939 516
Size: 423 3701
BsiEI CG_ry'CG
Cuts at: 104 140 756 1666 1712 2920 104
Size: 36 616 910 46 1208 1308
Fragments arranged by size:
1308 1208 910 616 46 36
BsiHKAI G_wGCw'C
Cuts at: 236 473 536 956 1138 1916 2106 2898 236
Size: 237 63 420 182 778 190 792 1462
Fragments arranged by size:
1462 792 778 420 237 190 182 63
Bsp1286I G_dGCh'C
Cuts at: 236 473 536 634 956 1138 1749 1842 1916
Size: 237 63 98 322 182 611 93 74
Cuts at: 1916 2106 2168 2898 236
Size: 190 62 730 1462
Fragments arranged by size:
1462 730 611 322 237 190 182 98
93 74 63 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 401 2416 2470 3954 4119 50
Size: 351 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 351 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 997 2122 2303 2512 997
Size: 1125 181 209 2609
Fragments arranged by size:
2609 1125 209 181
BsrGI T'GTAC_A
Cuts at: 386 386
Size: 4124
BssHII G'CGCG_C
Cuts at: 657 944 657
Size: 287 3837
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstYI r'GATC_y
Cuts at: 1 1149 1616 1639 1974 2220 3232 3318 3330
Size: 1148 467 23 335 246 1012 86 12
Cuts at: 3330 3676 1
Size: 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 568 568
Size: 4124
BtgI C'CryG_G
Cuts at: 107 286 596 1112 107
Size: 179 310 516 3119
Fragments arranged by size:
3119 516 310 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 517 1367 517
Size: 850 3274
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DraIII CAC_nnn'GTG
Cuts at: 917 917
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 137 1709 1883 2274 2301 3646 137
Size: 1572 174 391 27 1345 615
Fragments arranged by size:
1572 1345 615 391 174 27
EagI C'GGCC_G
Cuts at: 137 1709 137
Size: 1572 2552
EarI CTCTTCn'nnn_
Cuts at: 175 794 848 2147 2357 175
Size: 619 54 1299 210 1942
Fragments arranged by size:
1942 1299 619 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 598 970 1033 1949 2381 3128 3396 110
Size: 488 372 63 916 432 747 268 838
Fragments arranged by size:
916 838 747 488 432 372 268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 125 125
Size: 4124
EcoO109I rG'GnC_Cy
Cuts at: 302 309 630 631 1263 302
Size: 7 321 1 632 3163
Fragments arranged by size:
3163 632 321 7 1
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 118 216 367 1558 1806 2828 3807 118
Size: 98 151 1191 248 1022 979 435
Fragments arranged by size:
1191 1022 979 435 248 151 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1966 1996 1998 2028 1966
Size: 30 2 30 4062
Fragments arranged by size:
4062 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
HindIII A'AGCT_T
Cuts at: 1045 1045
Size: 4124
KasI G'GCGC_C
Cuts at: 363 1554 1802 363
Size: 1191 248 2685
Fragments arranged by size:
2685 1191 248
KpnI G_GTAC'C
Cuts at: 576 1144 576
Size: 568 3556
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 595 870 2113 2240 595
Size: 275 1243 127 2479
Fragments arranged by size:
2479 1243 275 127
MspA1I CmG'CkG
Cuts at: 254 288 320 442 504 737 1235 1549 1909
Size: 34 32 122 62 233 498 314 360
Cuts at: 1909 2922 3167 254
Size: 1013 245 1211
Fragments arranged by size:
1211 1013 498 360 314 245 233 122
62 34 32
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 364 1555 1803 364
Size: 1191 248 2685
Fragments arranged by size:
2685 1191 248
NcoI C'CATG_G
Cuts at: 107 596 1112 107
Size: 489 516 3119
Fragments arranged by size:
3119 516 489
NdeI CA'TA_TG
Cuts at: 880 880
Size: 4124
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PflMI CCAn_nnn'nTGG
Cuts at: 840 840
Size: 4124
PfoI T'CCnGG_A
Cuts at: 126 509 126
Size: 383 3741
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PspOMI G'GGCC_C
Cuts at: 630 630
Size: 4124
PstI C_TGCA'G
Cuts at: 201 1059 1171 1856 201
Size: 858 112 685 2469
Fragments arranged by size:
2469 858 685 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 320 737 1549 1909 320
Size: 417 812 360 2535
Fragments arranged by size:
2535 812 417 360
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SacII CC_GC'GG
Cuts at: 289 289
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 4124
SfcI C'TryA_G
Cuts at: 33 197 1055 1167 1852 2845 3036 33
Size: 164 858 112 685 993 191 1121
Fragments arranged by size:
1121 993 858 685 191 164 112
SfoI GGC'GCC
Cuts at: 365 1556 1804 365
Size: 1191 248 2685
Fragments arranged by size:
2685 1191 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 307 1146 307
Size: 839 3285
SmlI C'TyrA_G
Cuts at: 490 1279 2194 2686 2948 490
Size: 789 915 492 262 1666
Fragments arranged by size:
1666 915 789 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StuI AGG'CCT
Cuts at: 203 892 203
Size: 689 3435
StyI C'CwwG_G
Cuts at: 107 584 596 1112 1258 1612 107
Size: 477 12 516 146 354 2619
Fragments arranged by size:
2619 516 477 354 146 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1707 1840 2299 2508 1707
Size: 133 459 209 3323
Fragments arranged by size:
3323 459 209 133
TatI w'GTAC_w
Cuts at: 386 3639 386
Size: 3253 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 742 774 3641 3673 742
Size: 32 2867 32 1193
Fragments arranged by size:
2867 1193 32 32
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1026 1619 1026
Size: 593 3531
XmaI C'CCGG_G
Cuts at: 305 1144 305
Size: 839 3285
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BstEII BstXI BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI