Restriction map for
pF1KB9661
(Circular) (Six-base) MAPSORT of: pF1KB9661.seq Check: 7569 from: 1 to: 4142
pF1KB9661 4142 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1967 2399 3146 3414 1967
Size: 432 747 268 2695
Fragments arranged by size:
2695 747 432 268
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 692 692
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 1447 3014 1447
Size: 1567 2575
ApaI G_GGCC'C
Cuts at: 652 652
Size: 4142
ApaLI G'TGCA_C
Cuts at: 150 962 2912 150
Size: 812 1950 1380
Fragments arranged by size:
1950 1380 812
ApoI r'AATT_y
Cuts at: 760 1146 3495 3751 760
Size: 386 2349 256 1151
Fragments arranged by size:
2349 1151 386 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 385 664 1162 385
Size: 279 498 3365
Fragments arranged by size:
3365 498 279
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 1158 1572 1820 1855 1158
Size: 414 248 35 3445
Fragments arranged by size:
3445 414 248 35
BanII G_rGCy'C
Cuts at: 227 386 652 1156 2186 227
Size: 159 266 504 1030 2183
Fragments arranged by size:
2183 1030 504 266 159
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbsI GAAGACnn'nnnn_
Cuts at: 319 1017 1112 319
Size: 698 95 3349
Fragments arranged by size:
3349 698 95
BbvCI CC'TCA_GC
Cuts at: 538 538
Size: 4142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 943 977 1102 1136 943
Size: 34 125 34 3949
Fragments arranged by size:
3949 125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 600 2040 2801 4142 600
Size: 1440 761 1341 600
Fragments arranged by size:
1440 1341 761 600
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglII A'GATC_T
Cuts at: 560 1657 3694 560
Size: 1097 2037 1008
Fragments arranged by size:
2037 1097 1008
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 154 652 966 1767 1860 2916 154
Size: 498 314 801 93 1056 1380
Fragments arranged by size:
1380 1056 801 498 314 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 1354 1435 1770 3914 916
Size: 438 81 335 2144 1144
Fragments arranged by size:
2144 1144 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 110
Size: 4142
Bpu10I CC'TnA_GC
Cuts at: 538 538
Size: 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 1318 2197 2689 2987 375
Size: 943 879 492 298 1530
Fragments arranged by size:
1530 943 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3846 3846
Size: 4142
BsaAI yAC'GTr
Cuts at: 676 1495 676
Size: 819 3323
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 1022 1573 1821 2523 4019 1022
Size: 551 248 702 1496 1145
Fragments arranged by size:
1496 1145 702 551 248
BsaWI w'CCGG_w
Cuts at: 273 1331 1852 2530 2804 2951 3362 273
Size: 1058 521 678 274 147 411 1053
Fragments arranged by size:
1058 1053 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 4142
BseYI C'CCAG_C
Cuts at: 689 1567 2325 2902 3398 689
Size: 878 758 577 496 1433
Fragments arranged by size:
1433 878 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 957 243
Size: 714 3428
BsiEI CG_ry'CG
Cuts at: 104 274 1684 1730 2938 104
Size: 170 1410 46 1208 1308
Fragments arranged by size:
1410 1308 1208 170 46
BsiHKAI G_wGCw'C
Cuts at: 154 966 1156 1934 2124 2916 154
Size: 812 190 778 190 792 1380
Fragments arranged by size:
1380 812 792 778 190 190
Bsp1286I G_dGCh'C
Cuts at: 154 227 386 652 966 1156 1767 1860 1934
Size: 73 159 266 314 190 611 93 74
Cuts at: 1934 2124 2186 2916 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 730 611 314 266 190 190 159
93 74 73 62
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1192 1406 1708 2089 1192
Size: 214 302 381 3245
Fragments arranged by size:
3245 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 627 2054 627
Size: 1427 2715
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstEII G'GTnAC_C
Cuts at: 288 288
Size: 4142
BstYI r'GATC_y
Cuts at: 1 524 560 1167 1634 1657 1992 2238 3250
Size: 523 36 607 467 23 335 246 1012
Cuts at: 3250 3336 3348 3694 1
Size: 86 12 346 449
Fragments arranged by size:
1012 607 523 467 449 346 335 246
86 36 23 12
BtgI C'CryG_G
Cuts at: 107 431 107
Size: 324 3818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 506 881 2092 2273 2436 506
Size: 375 1211 181 163 2212
Fragments arranged by size:
2212 1211 375 181 163
BtsI GCAGTG_nn'
Cuts at: 964 1385 964
Size: 421 3721
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DraIII CAC_nnn'GTG
Cuts at: 935 935
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 1727 1901 2292 2319 3664 1727
Size: 174 391 27 1345 2205
Fragments arranged by size:
2205 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 359 365 633 2165 2375 359
Size: 6 268 1532 210 2126
Fragments arranged by size:
2126 1532 268 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 192 2350 2660 2806 3952 192
Size: 2158 310 146 1146 382
Fragments arranged by size:
2158 1146 382 310 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 1967 2399 3146 3414 110
Size: 1857 432 747 268 838
Fragments arranged by size:
1857 838 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 648 649 1281 648
Size: 1 632 3509
Fragments arranged by size:
3509 632 1
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 207 664 1576 1824 2846 3825 207
Size: 457 912 248 1022 979 524
Fragments arranged by size:
1022 979 912 524 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 282 314 1984 2014 2016 2046 282
Size: 32 1670 30 2 30 2378
Fragments arranged by size:
2378 1670 32 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1118 2813 2997 1118
Size: 1695 184 2263
Fragments arranged by size:
2263 1695 184
MscI TGG'CCA
Cuts at: 1903 1903
Size: 4142
MslI CAynn'nnrTG
Cuts at: 674 888 2131 2258 674
Size: 214 1243 127 2558
Fragments arranged by size:
2558 1243 214 127
MspA1I CmG'CkG
Cuts at: 460 755 1253 1567 1927 2940 3185 460
Size: 295 498 314 360 1013 245 1417
Fragments arranged by size:
1417 1013 498 360 314 295 245
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NcoI C'CATG_G
Cuts at: 107 107
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 1195 1695 2602 1195
Size: 500 907 2735
Fragments arranged by size:
2735 907 500
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PfoI T'CCnGG_A
Cuts at: 126 527 631 126
Size: 401 104 3637
Fragments arranged by size:
3637 401 104
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PshAI GACnn'nnGTC
Cuts at: 460 460
Size: 4142
PsiI TTA'TAA
Cuts at: 407 407
Size: 4142
PspOMI G'GGCC_C
Cuts at: 648 648
Size: 4142
PstI C_TGCA'G
Cuts at: 971 1189 1874 971
Size: 218 685 3239
Fragments arranged by size:
3239 685 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 460 755 1567 1927 460
Size: 295 812 360 2675
Fragments arranged by size:
2675 812 360 295
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 967 1073 1185 1870 2863 3054 33
Size: 934 106 112 685 993 191 1121
Fragments arranged by size:
1121 993 934 685 191 112 106
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 354 1297 2212 2704 2966 354
Size: 943 915 492 262 1530
Fragments arranged by size:
1530 943 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 107 602 1276 1630 107
Size: 495 674 354 2619
Fragments arranged by size:
2619 674 495 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 782 1725 1858 2317 2526 782
Size: 943 133 459 209 2398
Fragments arranged by size:
2398 943 459 209 133
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 133 154 165 3659 3691 122
Size: 11 21 11 3494 32 573
Fragments arranged by size:
3494 573 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1015 1637 1015
Size: 622 3520
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI