Restriction map for
pF1KB9658
(Circular) (Six-base) MAPSORT of: pF1KB9658.seq Check: 9761 from: 1 to: 4082
pF1KB9658 4082 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 797 924 797
Size: 127 3955
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 844 865 1008 1907 2339 3086 3354 309
Size: 535 21 143 899 432 747 268 1037
Fragments arranged by size:
1037 899 747 535 432 268 143 21
AfeI AGC'GCT
Cuts at: 437 437
Size: 4082
AflII C'TTAA_G
Cuts at: 946 946
Size: 4082
AflIII A'CryG_T
Cuts at: 536 882 1434 2538 3515 536
Size: 346 552 1104 977 1103
Fragments arranged by size:
1104 1103 977 552 346
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 242 468 1387 2954 242
Size: 226 919 1567 1370
Fragments arranged by size:
1567 1370 919 226
ApaI G_GGCC'C
Cuts at: 577 677 577
Size: 100 3982
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 352 397 418 543 670 676 1063 1102 352
Size: 45 21 125 127 6 387 39 3332
Fragments arranged by size:
3332 387 127 125 45 39 21 6
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 547 1098 1512 1760 1795 547
Size: 551 414 248 35 2834
Fragments arranged by size:
2834 551 414 248 35
BanII G_rGCy'C
Cuts at: 577 677 1039 1096 2126 577
Size: 100 362 57 1030 2533
Fragments arranged by size:
2533 1030 362 100 57
BbeI G_GCGC'C
Cuts at: 551 1516 1764 551
Size: 965 248 2869
Fragments arranged by size:
2869 965 248
BbsI GAAGACnn'nnnn_
Cuts at: 349 922 349
Size: 573 3509
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglI GCCn_nnn'nGGC
Cuts at: 216 556 216
Size: 340 3742
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 121 286 1194 121
Size: 165 908 3009
Fragments arranged by size:
3009 908 165
Bme1580I G_kGCm'C
Cuts at: 311 577 677 1707 1800 2856 311
Size: 266 100 1030 93 1056 1537
Fragments arranged by size:
1537 1056 1030 266 100 93
BmgBI CAC'GTC
Cuts at: 198 198
Size: 4082
BmrI ACTGGGnnnn_n'
Cuts at: 223 448 858 1294 1375 1710 3854 223
Size: 225 410 436 81 335 2144 451
Fragments arranged by size:
2144 451 436 410 335 225 81
BmtI G_CTAG'C
Cuts at: 641 641
Size: 4082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1053 2449 1053
Size: 1396 2686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 925
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1258 2137 2629 2927 172
Size: 1086 879 492 298 1327
Fragments arranged by size:
1327 1086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3786 3786
Size: 4082
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 415 548 983 1513 1761 2463 3959 415
Size: 133 435 530 248 702 1496 538
Fragments arranged by size:
1496 702 538 530 435 248 133
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 724 754 1039 1069 724
Size: 30 285 30 3737
Fragments arranged by size:
3737 285 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1047 1047
Size: 4082
BseYI C'CCAG_C
Cuts at: 319 570 1507 2265 2842 3338 319
Size: 251 937 758 577 496 1063
Fragments arranged by size:
1063 937 758 577 496 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 228
Size: 4082
BsiEI CG_ry'CG
Cuts at: 104 335 1624 1670 2878 104
Size: 231 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 231 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 974 974
Size: 4082
Bsp1286I G_dGCh'C
Cuts at: 311 577 677 1039 1096 1707 1800 1874 2064
Size: 266 100 362 57 611 93 74 190
Cuts at: 2064 2126 2856 311
Size: 62 730 1537
Fragments arranged by size:
1537 730 611 362 266 190 100 93
74 62 57
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 797 863 924 1132 1346 1648 2029 191
Size: 606 66 61 208 214 302 381 2244
Fragments arranged by size:
2244 606 381 302 214 208 66 61
BsrBI CCG'CTC
Cuts at: 50 285 2374 2428 3912 4077 50
Size: 235 2089 54 1484 165 55
Fragments arranged by size:
2089 1484 235 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 262 550 2080 2261 2470 262
Size: 288 1530 181 209 1874
Fragments arranged by size:
1874 1530 288 209 181
BssHII G'CGCG_C
Cuts at: 219 219
Size: 4082
BssSI C'ACGA_G
Cuts at: 754 2353 2711 754
Size: 1599 358 2125
Fragments arranged by size:
2125 1599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 242 456 654 1459 242
Size: 214 198 805 2865
Fragments arranged by size:
2865 805 214 198
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 153 153
Size: 4082
BstYI r'GATC_y
Cuts at: 1 357 1107 1574 1597 1932 2178 3190 3276
Size: 356 750 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 750 467 449 356 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 113 158 107
Size: 6 45 4031
Fragments arranged by size:
4031 45 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DraIII CAC_nnn'GTG
Cuts at: 296 456 296
Size: 160 3922
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 110 155 1667 1841 2232 2259 3604 110
Size: 45 1512 174 391 27 1345 588
Fragments arranged by size:
1512 1345 588 391 174 45 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 808 852 2290 2600 2746 3892 808
Size: 44 1438 310 146 1146 998
Fragments arranged by size:
1438 1146 998 310 146 44
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 844 865 925 1008 1907 2339 3086 3354
Size: 535 21 60 83 899 432 747 268
Cuts at: 3354 309
Size: 1037
Fragments arranged by size:
1037 899 747 535 432 268 83 60
21
EcoO109I rG'GnC_Cy
Cuts at: 239 1221 239
Size: 982 3100
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 406 439 497 551 635 1516 1764 2786 3765
Size: 33 58 54 84 881 248 1022 979
Cuts at: 3765 406
Size: 723
Fragments arranged by size:
1022 979 881 723 248 84 58 54
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 724 756 1039 1071 1924 1954 1956 1986 724
Size: 32 283 32 853 30 2 30 2820
Fragments arranged by size:
2820 853 283 32 32 30 30 2
HincII GTy'rAC
Cuts at: 171 1121 171
Size: 950 3132
HindIII A'AGCT_T
Cuts at: 474 474
Size: 4082
KasI G'GCGC_C
Cuts at: 547 1512 1760 547
Size: 965 248 2869
Fragments arranged by size:
2869 965 248
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 536 882 3515 536
Size: 346 2633 1103
Fragments arranged by size:
2633 1103 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 767 2753 2937 767
Size: 1986 184 1912
Fragments arranged by size:
1986 1912 184
MscI TGG'CCA
Cuts at: 157 1843 157
Size: 1686 2396
MslI CAynn'nnrTG
Cuts at: 766 2071 2198 766
Size: 1305 127 2650
Fragments arranged by size:
2650 1305 127
MspA1I CmG'CkG
Cuts at: 115 153 468 654 1193 1507 1867 2880 3125
Size: 38 315 186 539 314 360 1013 245
Cuts at: 3125 115
Size: 1072
Fragments arranged by size:
1072 1013 539 360 315 314 245 186
38
NaeI GCC'GGC
Cuts at: 264 552 2263 264
Size: 288 1711 2083
Fragments arranged by size:
2083 1711 288
NarI GG'CG_CC
Cuts at: 548 1513 1761 548
Size: 965 248 2869
Fragments arranged by size:
2869 965 248
NcoI C'CATG_G
Cuts at: 107 158 107
Size: 51 4031
NgoMIV G'CCGG_C
Cuts at: 262 550 2261 262
Size: 288 1711 2083
Fragments arranged by size:
2083 1711 288
NheI G'CTAG_C
Cuts at: 637 637
Size: 4082
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PspOMI G'GGCC_C
Cuts at: 573 673 573
Size: 100 3982
PspXI vC'TCGA_Gb
Cuts at: 352 352
Size: 4082
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 841 873 841
Size: 32 4050
PstI C_TGCA'G
Cuts at: 257 1129 1814 257
Size: 872 685 2525
Fragments arranged by size:
2525 872 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 654 1507 1867 654
Size: 853 360 2869
Fragments arranged by size:
2869 853 360
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SacII CC_GC'GG
Cuts at: 116 116
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 253 1125 1810 2803 2994 33
Size: 220 872 685 993 191 1121
Fragments arranged by size:
1121 993 872 685 220 191
SfoI GGC'GCC
Cuts at: 549 1514 1762 549
Size: 965 248 2869
Fragments arranged by size:
2869 965 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SgrAI Cr'CCGG_yG
Cuts at: 550 550
Size: 4082
SmaI CCC'GGG
Cuts at: 545 672 678 1104 545
Size: 127 6 426 3523
Fragments arranged by size:
3523 426 127 6
SmlI C'TyrA_G
Cuts at: 187 352 946 1237 2152 2644 2906 187
Size: 165 594 291 915 492 262 1363
Fragments arranged by size:
1363 915 594 492 291 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
SrfI GCCC'GGGC
Cuts at: 678 678
Size: 4082
StyI C'CwwG_G
Cuts at: 107 158 803 1216 1570 107
Size: 51 645 413 354 2619
Fragments arranged by size:
2619 645 413 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 467 1665 1798 2257 2466 349
Size: 118 1198 133 459 209 1965
Fragments arranged by size:
1965 1198 459 209 133 118
TatI w'GTAC_w
Cuts at: 362 3597 362
Size: 3235 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1577 150
Size: 1427 2655
XhoI C'TCGA_G
Cuts at: 352 352
Size: 4082
XmaI C'CCGG_G
Cuts at: 543 670 676 1102 543
Size: 127 6 426 3523
Fragments arranged by size:
3523 426 127 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AhdI AleI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI Bpu10I BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI SanDI SexAI SfiI
SnaBI SspI StuI SwaI XmnI ZraI