Restriction map for
pF1KB9657
(Circular) (Six-base) MAPSORT of: pF1KB9657.seq Check: 4508 from: 1 to: 3719
pF1KB9657 3719 bp
With 182 enzymes: SgfI *
May 10, 2010 11:26 ..
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AflIII A'CryG_T
Cuts at: 119 1071 2175 3152 119
Size: 952 1104 977 686
Fragments arranged by size:
1104 977 952 686
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 477 1024 2591 477
Size: 547 1567 1605
Fragments arranged by size:
1605 1567 547
ApaI G_GGCC'C
Cuts at: 263 263
Size: 3719
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 739 739
Size: 3719
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 263 733 1763 263
Size: 470 1030 2219
Fragments arranged by size:
2219 1030 470
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglI GCCn_nnn'nGGC
Cuts at: 183 183
Size: 3719
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 535 655 831 535
Size: 120 176 3423
Fragments arranged by size:
3423 176 120
Bme1580I G_kGCm'C
Cuts at: 263 1344 1437 2493 263
Size: 1081 93 1056 1489
Fragments arranged by size:
1489 1081 1056 93
BmgBI CAC'GTC
Cuts at: 120 120
Size: 3719
BmrI ACTGGGnnnn_n'
Cuts at: 465 931 1012 1347 3491 465
Size: 466 81 335 2144 693
Fragments arranged by size:
2144 693 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 630 2086 630
Size: 1456 2263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 696 580
Size: 116 3603
Bpu10I CC'TnA_GC
Cuts at: 157 157
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 895 1774 2266 2564 538
Size: 357 879 492 298 1693
Fragments arranged by size:
1693 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 284 1150 1398 2100 3596 284
Size: 866 248 702 1496 407
Fragments arranged by size:
1496 866 702 407 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 433
Size: 3719
BseYI C'CCAG_C
Cuts at: 1144 1902 2479 2975 1144
Size: 758 577 496 1888
Fragments arranged by size:
1888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 146 1261 1307 2515 104
Size: 42 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 46 42
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 263 733 1344 1437 1511 1701 1763 2493 263
Size: 470 611 93 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 470 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 983 1285 1666 769
Size: 214 302 381 2822
Fragments arranged by size:
2822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 200 651 2011 2065 3549 3714 50
Size: 150 451 1360 54 1484 165 55
Fragments arranged by size:
1484 1360 451 165 150 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 141 182 1717 1898 2107 141
Size: 41 1535 181 209 1753
Fragments arranged by size:
1753 1535 209 181 41
BssSI C'ACGA_G
Cuts at: 646 1990 2348 646
Size: 1344 358 2017
Fragments arranged by size:
2017 1344 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstYI r'GATC_y
Cuts at: 1 744 1211 1234 1569 1815 2827 2913 2925
Size: 743 467 23 335 246 1012 86 12
Cuts at: 2925 3271 1
Size: 346 449
Fragments arranged by size:
1012 743 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 272 272
Size: 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 143 443 563 1304 1478 1869 1896 3241 143
Size: 300 120 741 174 391 27 1345 621
Fragments arranged by size:
1345 741 621 391 300 174 120 27
EagI C'GGCC_G
Cuts at: 143 1304 143
Size: 1161 2558
EarI CTCTTCn'nnn_
Cuts at: 167 1742 1952 167
Size: 1575 210 1934
Fragments arranged by size:
1934 1575 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 281 1927 2237 2383 3529 281
Size: 1646 310 146 1146 471
Fragments arranged by size:
1646 1146 471 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 696 1544 1976 2723 2991 580
Size: 116 848 432 747 268 1308
Fragments arranged by size:
1308 848 747 432 268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 586 586
Size: 3719
EcoO109I rG'GnC_Cy
Cuts at: 259 591 689 858 259
Size: 332 98 169 3120
Fragments arranged by size:
3120 332 169 98
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 462 1153 1401 2423 3402 462
Size: 691 248 1022 979 779
Fragments arranged by size:
1022 979 779 691 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1561 1591 1593 1623 1561
Size: 30 2 30 3657
Fragments arranged by size:
3657 30 30 2
HincII GTy'rAC
Cuts at: 758 758
Size: 3719
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 267 2390 2574 267
Size: 2123 184 1412
Fragments arranged by size:
2123 1412 184
MscI TGG'CCA
Cuts at: 445 1480 445
Size: 1035 2684
MslI CAynn'nnrTG
Cuts at: 1708 1835 1708
Size: 127 3592
MspA1I CmG'CkG
Cuts at: 274 392 435 830 1144 1504 2517 2762 274
Size: 118 43 395 314 360 1013 245 1231
Fragments arranged by size:
1231 1013 395 360 314 245 118 43
NaeI GCC'GGC
Cuts at: 143 1900 143
Size: 1757 1962
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NgoMIV G'CCGG_C
Cuts at: 141 1898 141
Size: 1757 1962
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PasI CC'CwG_GG
Cuts at: 318 318
Size: 3719
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 591 591
Size: 3719
PspOMI G'GGCC_C
Cuts at: 259 259
Size: 3719
PstI C_TGCA'G
Cuts at: 428 634 766 1451 428
Size: 206 132 685 2696
Fragments arranged by size:
2696 685 206 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SacII CC_GC'GG
Cuts at: 275 275
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 167 1742 1952 167
Size: 1575 210 1934
Fragments arranged by size:
1934 1575 210
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SexAI A'CCwGG_T
Cuts at: 587 587
Size: 3719
SfcI C'TryA_G
Cuts at: 33 424 630 762 1447 2440 2631 33
Size: 391 206 132 685 993 191 1121
Fragments arranged by size:
1121 993 685 391 206 191 132
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 741 741
Size: 3719
SmlI C'TyrA_G
Cuts at: 553 874 1789 2281 2543 553
Size: 321 915 492 262 1729
Fragments arranged by size:
1729 915 492 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 853 1207 853
Size: 354 3365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 660 1302 1435 1894 2103 484
Size: 176 642 133 459 209 2100
Fragments arranged by size:
2100 642 459 209 176 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1214 1214
Size: 3719
XmaI C'CCGG_G
Cuts at: 739 739
Size: 3719
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI