Restriction map for
pF1KB9656
(Circular) (Six-base) MAPSORT of: pF1KB9656.seq Check: 7829 from: 1 to: 3590
pF1KB9656 3590 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 628 628
Size: 3590
Acc65I G'GTAC_C
Cuts at: 606 606
Size: 3590
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
AflIII A'CryG_T
Cuts at: 942 2046 3023 942
Size: 1104 977 1509
Fragments arranged by size:
1509 1104 977
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 3558
AlwNI CAG_nnn'CTG
Cuts at: 895 2462 895
Size: 1567 2023
ApaLI G'TGCA_C
Cuts at: 2360 2360
Size: 3590
ApoI r'AATT_y
Cuts at: 468 594 2943 3199 468
Size: 126 2349 256 859
Fragments arranged by size:
2349 859 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 208 610 208
Size: 402 3188
BamHI G'GATC_C
Cuts at: 615 615
Size: 3590
BanI G'GyrC_C
Cuts at: 350 606 1020 1268 1303 350
Size: 256 414 248 35 2637
Fragments arranged by size:
2637 414 256 248 35
BanII G_rGCy'C
Cuts at: 266 278 604 1634 266
Size: 12 326 1030 2222
Fragments arranged by size:
2222 1030 326 12
BbeI G_GCGC'C
Cuts at: 354 1024 1272 354
Size: 670 248 2672
Fragments arranged by size:
2672 670 248
BbsI GAAGACnn'nnnn_
Cuts at: 212 212
Size: 3590
BciVI GTATCCnnnnn_n'
Cuts at: 1488 2249 3590 1488
Size: 761 1341 1488
Fragments arranged by size:
1488 1341 761
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 702 702
Size: 3590
Bme1580I G_kGCm'C
Cuts at: 1215 1308 2364 1215
Size: 93 1056 2441
Fragments arranged by size:
2441 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 883 1218 3362 802
Size: 81 335 2144 1030
Fragments arranged by size:
2144 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 1957 252
Size: 1705 1885
Bpu10I CC'TnA_GC
Cuts at: 272 272
Size: 3590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1645 2137 2435 766
Size: 879 492 298 1921
Fragments arranged by size:
1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3294 3294
Size: 3590
BsaAI yAC'GTr
Cuts at: 943 943
Size: 3590
BsaBI GATnn'nnATC
Cuts at: 6 1104 3141 6
Size: 1098 2037 455
Fragments arranged by size:
2037 1098 455
BsaHI Gr'CG_yC
Cuts at: 351 1021 1269 1971 3467 351
Size: 670 248 702 1496 474
Fragments arranged by size:
1496 702 670 474 248
BsaWI w'CCGG_w
Cuts at: 177 779 1300 1978 2252 2399 2810 177
Size: 602 521 678 274 147 411 957
Fragments arranged by size:
957 678 602 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 232 247 262 217
Size: 15 15 15 3545
Fragments arranged by size:
3545 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 226 241 256 259 274 283 316 496
Size: 45 15 15 3 15 9 33 180
Cuts at: 496 181
Size: 3275
Fragments arranged by size:
3275 180 45 33 15 15 15 9
3
BseYI C'CCAG_C
Cuts at: 1015 1773 2350 2846 1015
Size: 758 577 496 1759
Fragments arranged by size:
1759 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1132 1178 2386 104
Size: 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 188 266 278 604 1382 1572 2364 188
Size: 78 12 326 778 190 792 1414
Fragments arranged by size:
1414 792 778 326 190 78 12
Bsp1286I G_dGCh'C
Cuts at: 188 266 278 604 1215 1308 1382 1572 1634
Size: 78 12 326 611 93 74 190 62
Cuts at: 1634 2364 188
Size: 730 1414
Fragments arranged by size:
1414 730 611 326 190 93 78 74
62 12
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 3590
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 854 1156 1537 640
Size: 214 302 381 2693
Fragments arranged by size:
2693 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1882 1936 3420 3585 50
Size: 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 483 1502 483
Size: 1019 2571
BsrFI r'CCGG_y
Cuts at: 1588 1769 1978 1588
Size: 181 209 3200
Fragments arranged by size:
3200 209 181
BssHII G'CGCG_C
Cuts at: 149 149
Size: 3590
BssSI C'ACGA_G
Cuts at: 1861 2219 1861
Size: 358 3232
BstAPI GCAn_nnn'nTGC
Cuts at: 967 967
Size: 3590
BstBI TT'CG_AA
Cuts at: 1951 1951
Size: 3590
BstYI r'GATC_y
Cuts at: 1 615 1082 1105 1440 1686 2698 2784 2796
Size: 614 467 23 335 246 1012 86 12
Cuts at: 2796 3142 1
Size: 346 449
Fragments arranged by size:
1012 614 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 257 257
Size: 3590
BtgI C'CryG_G
Cuts at: 107 359 107
Size: 252 3338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1540 1721 1884 1540
Size: 181 163 3246
Fragments arranged by size:
3246 181 163
BtsI GCAGTG_nn'
Cuts at: 833 833
Size: 3590
DraI TTT'AAA
Cuts at: 414 589 414
Size: 175 3415
DrdI GACnn_nn'nnGTC
Cuts at: 1296 2154 1296
Size: 858 2732
EaeI y'GGCC_r
Cuts at: 1175 1349 1740 1767 3112 1175
Size: 174 391 27 1345 1653
Fragments arranged by size:
1653 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 131 506 1613 1823 131
Size: 375 1107 210 1898
Fragments arranged by size:
1898 1107 375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 264 276 602 264
Size: 12 326 3252
Fragments arranged by size:
3252 326 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 298 343 298
Size: 45 3545
EcoO109I rG'GnC_Cy
Cuts at: 336 729 336
Size: 393 3197
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 309 354 1024 1272 2294 3273 309
Size: 45 670 248 1022 979 626
Fragments arranged by size:
1022 979 670 626 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 217 234 249 1432 1462 1464 1494 202
Size: 15 17 15 1183 30 2 30 2298
Fragments arranged by size:
2298 1183 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 629 629
Size: 3590
HindIII A'AGCT_T
Cuts at: 394 408 394
Size: 14 3576
KasI G'GCGC_C
Cuts at: 350 1020 1268 350
Size: 670 248 2672
Fragments arranged by size:
2672 670 248
KpnI G_GTAC'C
Cuts at: 610 610
Size: 3590
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 1351 1351
Size: 3590
MslI CAynn'nnrTG
Cuts at: 1579 1706 1579
Size: 127 3463
MspA1I CmG'CkG
Cuts at: 701 1015 1375 2388 2633 701
Size: 314 360 1013 245 1658
Fragments arranged by size:
1658 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1771 1771
Size: 3590
NarI GG'CG_CC
Cuts at: 351 1021 1269 351
Size: 670 248 2672
Fragments arranged by size:
2672 670 248
NcoI C'CATG_G
Cuts at: 107 359 107
Size: 252 3338
NgoMIV G'CCGG_C
Cuts at: 1769 1769
Size: 3590
NspI r_CATG'y
Cuts at: 643 1143 2050 643
Size: 500 907 2183
Fragments arranged by size:
2183 907 500
PciI A'CATG_T
Cuts at: 2046 2046
Size: 3590
PfoI T'CCnGG_A
Cuts at: 328 328
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 232 264 2729 2761 232
Size: 32 2465 32 1061
Fragments arranged by size:
2465 1061 32 32
PpuMI rG'GwC_Cy
Cuts at: 336 336
Size: 3590
PstI C_TGCA'G
Cuts at: 637 1322 637
Size: 685 2905
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3590
PvuII CAG'CTG
Cuts at: 1015 1375 1015
Size: 360 3230
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 266 278 604 266
Size: 12 326 3252
Fragments arranged by size:
3252 326 12
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 131 1613 1823 131
Size: 1482 210 1898
Fragments arranged by size:
1898 1482 210
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 633 1318 2311 2502 33
Size: 600 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 191
SfoI GGC'GCC
Cuts at: 352 1022 1270 352
Size: 670 248 2672
Fragments arranged by size:
2672 670 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 745 1660 2152 2414 745
Size: 915 492 262 1921
Fragments arranged by size:
1921 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3590
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StuI AGG'CCT
Cuts at: 174 174
Size: 3590
StyI C'CwwG_G
Cuts at: 107 359 724 1078 107
Size: 252 365 354 2619
Fragments arranged by size:
2619 365 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 1173 1306 1765 1974 175
Size: 998 133 459 209 1791
Fragments arranged by size:
1791 998 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3558
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1085 1085
Size: 3590
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI