Restriction map for
pF1KB9655
(Circular) (Six-base) MAPSORT of: pF1KB9655.seq Check: 9873 from: 1 to: 5159
pF1KB9655 5159 bp
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AatII G_ACGT'C
Cuts at: 341 341
Size: 5159
AccI GT'mk_AC
Cuts at: 2197 2197
Size: 5159
Acc65I G'GTAC_C
Cuts at: 2175 2175
Size: 5159
AclI AA'CG_TT
Cuts at: 4999 4999
Size: 5159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2984 3416 4163 4431 2984
Size: 432 747 268 3712
Fragments arranged by size:
3712 747 432 268
AfeI AGC'GCT
Cuts at: 1885 1885
Size: 5159
AflIII A'CryG_T
Cuts at: 2511 3615 4592 2511
Size: 1104 977 3078
Fragments arranged by size:
3078 1104 977
AgeI A'CCGG_T
Cuts at: 3547 3547
Size: 5159
AleI CACnn'nnGTG
Cuts at: 946 2044 946
Size: 1098 4061
AlwNI CAG_nnn'CTG
Cuts at: 2464 4031 2464
Size: 1567 3592
ApaI G_GGCC'C
Cuts at: 1377 1465 1377
Size: 88 5071
ApaLI G'TGCA_C
Cuts at: 3929 3929
Size: 5159
ApoI r'AATT_y
Cuts at: 154 754 2163 4512 4768 154
Size: 600 1409 2349 256 545
Fragments arranged by size:
2349 1409 600 545 256
AscI GG'CGCG_CC
Cuts at: 1538 1538
Size: 5159
AseI AT'TA_AT
Cuts at: 20 20
Size: 5159
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
AvaI C'yCGr_G
Cuts at: 1369 1446 1465 1533 1992 2179 1369
Size: 77 19 68 459 187 4349
Fragments arranged by size:
4349 459 187 77 68 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 709 742 709
Size: 33 5126
BamHI G'GATC_C
Cuts at: 193 885 2184 193
Size: 692 1299 3168
Fragments arranged by size:
3168 1299 692
BanI G'GyrC_C
Cuts at: 705 764 1744 1771 1837 1903 2175 2589 2837
Size: 59 980 27 66 66 272 414 248
Cuts at: 2837 2872 705
Size: 35 2992
Fragments arranged by size:
2992 980 414 272 248 66 66 59
35 27
BanII G_rGCy'C
Cuts at: 669 999 1377 1465 1786 2173 3203 669
Size: 330 378 88 321 387 1030 2625
Fragments arranged by size:
2625 1030 387 378 330 321 88
BbeI G_GCGC'C
Cuts at: 1748 1841 1907 2593 2841 1748
Size: 93 66 686 248 4066
Fragments arranged by size:
4066 686 248 93 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 388 422 940 974 90
Size: 34 264 34 518 34 4275
Fragments arranged by size:
4275 518 264 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 604 3057 3818 5159 604
Size: 2453 761 1341 604
Fragments arranged by size:
2453 1341 761 604
BclI T'GATC_A
Cuts at: 779 2679 779
Size: 1900 3259
BfrBI ATG'CAT
Cuts at: 972 972
Size: 5159
BglI GCCn_nnn'nGGC
Cuts at: 747 1166 1715 1800 747
Size: 419 549 85 4106
Fragments arranged by size:
4106 549 419 85
BglII A'GATC_T
Cuts at: 2674 4711 2674
Size: 2037 3122
BlpI GC'TnA_GC
Cuts at: 2271 2271
Size: 5159
Bme1580I G_kGCm'C
Cuts at: 710 1370 1377 1465 2784 2877 3933 710
Size: 660 7 88 1319 93 1056 1936
Fragments arranged by size:
1936 1319 1056 660 93 88 7
BmrI ACTGGGnnnn_n'
Cuts at: 461 1001 2371 2452 2787 4931 461
Size: 540 1370 81 335 2144 689
Fragments arranged by size:
2144 1370 689 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1977 3526 1977
Size: 1549 3610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 265 918 1968 136
Size: 129 653 1050 3327
Fragments arranged by size:
3327 1050 653 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2335 3214 3706 4004 2053
Size: 282 879 492 298 3208
Fragments arranged by size:
3208 879 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 381 841 4863 381
Size: 460 4022 677
Fragments arranged by size:
4022 677 460
BsaAI yAC'GTr
Cuts at: 2512 2512
Size: 5159
BsaBI GATnn'nnATC
Cuts at: 6 781 890 2673 4710 6
Size: 775 109 1783 2037 455
Fragments arranged by size:
2037 1783 775 455 109
BsaHI Gr'CG_yC
Cuts at: 338 1567 1745 1838 1904 1916 1957 2590 2838
Size: 1229 178 93 66 12 41 633 248
Cuts at: 2838 3540 5036 338
Size: 702 1496 461
Fragments arranged by size:
1496 1229 702 633 461 248 178 93
66 41 12
BsaWI w'CCGG_w
Cuts at: 975 2348 2869 3547 3821 3968 4379 975
Size: 1373 521 678 274 147 411 1755
Fragments arranged by size:
1755 1373 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 697 637
Size: 30 30 5099
Fragments arranged by size:
5099 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 588 675 1873 1953 415
Size: 173 87 1198 80 3621
Fragments arranged by size:
3621 1198 173 87 80
BseYI C'CCAG_C
Cuts at: 692 1739 2087 2584 3342 3919 4415 692
Size: 1047 348 497 758 577 496 1436
Fragments arranged by size:
1436 1047 758 577 497 496 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1683 1195
Size: 488 4671
BsiEI CG_ry'CG
Cuts at: 104 623 1170 1820 2036 2701 2747 3955 104
Size: 519 547 650 216 665 46 1208 1308
Fragments arranged by size:
1308 1208 665 650 547 519 216 46
BsiHKAI G_wGCw'C
Cuts at: 122 669 2173 2951 3141 3933 122
Size: 547 1504 778 190 792 1348
Fragments arranged by size:
1504 1348 792 778 547 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1948 1948
Size: 5159
Bsp1286I G_dGCh'C
Cuts at: 122 669 710 999 1370 1377 1465 1786 2173
Size: 547 41 289 371 7 88 321 387
Cuts at: 2173 2784 2877 2951 3141 3203 3933 122
Size: 611 93 74 190 62 730 1348
Fragments arranged by size:
1348 730 611 547 387 371 321 289
190 93 88 74 62 41 7
BspEI T'CCGG_A
Cuts at: 975 4379 975
Size: 3404 1755
BspHI T'CATG_A
Cuts at: 4335 4335
Size: 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2209 2423 2725 3106 2209
Size: 214 302 381 4262
Fragments arranged by size:
4262 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1713 2067 3451 3505 4989 5154 50
Size: 1663 354 1384 54 1484 165 55
Fragments arranged by size:
1663 1484 1384 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3071 3071
Size: 5159
BsrFI r'CCGG_y
Cuts at: 501 622 761 1306 1390 1569 1580 1834 2031
Size: 121 139 545 84 179 11 254 197
Cuts at: 2031 3157 3338 3547 501
Size: 1126 181 209 2113
Fragments arranged by size:
2113 1126 545 254 209 197 181 179
139 121 84 11
BssHII G'CGCG_C
Cuts at: 1538 1825 1538
Size: 287 4872
BssSI C'ACGA_G
Cuts at: 3430 3788 3430
Size: 358 4801
BstAPI GCAn_nnn'nTGC
Cuts at: 1365 2536 1365
Size: 1171 3988
BstBI TT'CG_AA
Cuts at: 3520 3520
Size: 5159
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5159
BstYI r'GATC_y
Cuts at: 1 193 885 2024 2184 2651 2674 3009 3255
Size: 192 692 1139 160 467 23 335 246
Cuts at: 3255 4267 4353 4365 4711 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1139 1012 692 467 449 346 335 246
192 160 86 23 12
BtgI C'CryG_G
Cuts at: 617 845 1385 1616 1814 617
Size: 228 540 231 198 3962
Fragments arranged by size:
3962 540 231 228 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1191 3109 3290 3453 1191
Size: 1918 181 163 2897
Fragments arranged by size:
2897 1918 181 163
BtsI GCAGTG_nn'
Cuts at: 120 458 2402 120
Size: 338 1944 2877
Fragments arranged by size:
2877 1944 338
DraI TTT'AAA
Cuts at: 2158 2158
Size: 5159
DrdI GACnn_nn'nnGTC
Cuts at: 291 2865 3723 291
Size: 2574 858 1727
Fragments arranged by size:
2574 1727 858
EaeI y'GGCC_r
Cuts at: 455 489 620 648 1167 1388 1571 1613 1637
Size: 34 131 28 519 221 183 42 24
Cuts at: 1637 1704 1817 1832 1874 2033 2744 2918 3309
Size: 67 113 15 42 159 711 174 391
Cuts at: 3309 3336 4681 455
Size: 27 1345 933
Fragments arranged by size:
1345 933 711 519 391 221 183 174
159 131 113 67 42 42 34 28
27 24 15
EagI C'GGCC_G
Cuts at: 620 1167 1817 2033 2744 620
Size: 547 650 216 711 3035
Fragments arranged by size:
3035 711 650 547 216
EarI CTCTTCn'nnn_
Cuts at: 3182 3392 3182
Size: 210 4949
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 206 898 2037 3367 3677 3823 4969 206
Size: 692 1139 1330 310 146 1146 396
Fragments arranged by size:
1330 1146 1139 692 396 310 146
EcoICRI GAG'CTC
Cuts at: 667 2171 667
Size: 1504 3655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 265 918 1968 2984 3416 4163 4431 136
Size: 129 653 1050 1016 432 747 268 864
Fragments arranged by size:
1050 1016 864 747 653 432 268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 1855 1855
Size: 5159
EcoO109I rG'GnC_Cy
Cuts at: 740 1373 1462 1530 1869 1929 2298 740
Size: 633 89 68 339 60 369 3601
Fragments arranged by size:
3601 633 369 339 89 68 60
EcoRI G'AATT_C
Cuts at: 2163 2163
Size: 5159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 275 1455 3178 275
Size: 1180 1723 2256
Fragments arranged by size:
2256 1723 1180
FspI TGC'GCA
Cuts at: 2940 2940
Size: 5159
HaeII r_GCGC'y
Cuts at: 1678 1748 1814 1841 1887 1907 2593 2841 3863
Size: 70 66 27 46 20 686 248 1022
Cuts at: 3863 4842 1678
Size: 979 1995
Fragments arranged by size:
1995 1022 979 686 248 70 66 46
27 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 273 305 361 393 2044 2076 3001 3031 3033
Size: 32 56 32 1651 32 925 30 2
Cuts at: 3033 3063 273
Size: 30 2369
Fragments arranged by size:
2369 1651 925 56 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2198 2198
Size: 5159
KasI G'GCGC_C
Cuts at: 1744 1837 1903 2589 2837 1744
Size: 93 66 686 248 4066
Fragments arranged by size:
4066 686 248 93 66
KpnI G_GTAC'C
Cuts at: 2179 2179
Size: 5159
MluI A'CGCG_T
Cuts at: 4592 4592
Size: 5159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 3830 4014 617
Size: 3213 184 1762
Fragments arranged by size:
3213 1762 184
MscI TGG'CCA
Cuts at: 2920 2920
Size: 5159
MslI CAynn'nnrTG
Cuts at: 946 2044 3148 3275 946
Size: 1098 1104 127 2830
Fragments arranged by size:
2830 1104 1098 127
MspA1I CmG'CkG
Cuts at: 114 1683 1816 1893 1946 2091 2270 2584 2944
Size: 1569 133 77 53 145 179 314 360
Cuts at: 2944 3957 4202 114
Size: 1013 245 1071
Fragments arranged by size:
1569 1071 1013 360 314 245 179 145
133 77 53
NaeI GCC'GGC
Cuts at: 624 1392 1571 1836 2033 3340 624
Size: 768 179 265 197 1307 2443
Fragments arranged by size:
2443 1307 768 265 197 179
NarI GG'CG_CC
Cuts at: 1745 1838 1904 2590 2838 1745
Size: 93 66 686 248 4066
Fragments arranged by size:
4066 686 248 93 66
NcoI C'CATG_G
Cuts at: 1385 1385
Size: 5159
NgoMIV G'CCGG_C
Cuts at: 622 1390 1569 1834 2031 3338 622
Size: 768 179 265 197 1307 2443
Fragments arranged by size:
2443 1307 768 265 197 179
NotI GC'GGCC_GC
Cuts at: 1817 1817
Size: 5159
NsiI A_TGCA'T
Cuts at: 974 974
Size: 5159
NspI r_CATG'y
Cuts at: 807 1123 2212 2712 3619 807
Size: 316 1089 500 907 2347
Fragments arranged by size:
2347 1089 907 500 316
PciI A'CATG_T
Cuts at: 3615 3615
Size: 5159
PflMI CCAn_nnn'nTGG
Cuts at: 896 896
Size: 5159
PmeI GTTT'AAAC
Cuts at: 2158 2158
Size: 5159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 4298 4330 1186
Size: 32 3080 32 2015
Fragments arranged by size:
3080 2015 32 32
PpuMI rG'GwC_Cy
Cuts at: 1530 1929 1530
Size: 399 4760
PspOMI G'GGCC_C
Cuts at: 1373 1461 1373
Size: 88 5071
PstI C_TGCA'G
Cuts at: 414 1406 1688 1695 2206 2891 414
Size: 992 282 7 511 685 2682
Fragments arranged by size:
2682 992 685 511 282 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5159
PvuII CAG'CTG
Cuts at: 114 1946 2584 2944 114
Size: 1832 638 360 2329
Fragments arranged by size:
2329 1832 638 360
RsrII CG'GwC_CG
Cuts at: 499 1479 3354 499
Size: 980 1875 2304
Fragments arranged by size:
2304 1875 980
SacI G_AGCT'C
Cuts at: 669 2173 669
Size: 1504 3655
SacII CC_GC'GG
Cuts at: 1817 1817
Size: 5159
SalI G'TCGA_C
Cuts at: 2196 2196
Size: 5159
SapI GCTCTTCn'nnn_
Cuts at: 3182 3392 3182
Size: 210 4949
SbfI CC_TGCA'GG
Cuts at: 1406 2206 1406
Size: 800 4359
ScaI AGT'ACT
Cuts at: 1765 4676 1765
Size: 2911 2248
SfcI C'TryA_G
Cuts at: 33 410 1402 1684 1691 2202 2887 3880 4071
Size: 377 992 282 7 511 685 993 191
Cuts at: 4071 33
Size: 1121
Fragments arranged by size:
1121 993 992 685 511 377 282 191
7
SfoI GGC'GCC
Cuts at: 1746 1839 1905 2591 2839 1746
Size: 93 66 686 248 4066
Fragments arranged by size:
4066 686 248 93 66
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
SmaI CCC'GGG
Cuts at: 1371 1448 1467 1535 2181 1371
Size: 77 19 68 646 4349
Fragments arranged by size:
4349 646 77 68 19
SmlI C'TyrA_G
Cuts at: 2068 2314 3229 3721 3983 2068
Size: 246 915 492 262 3244
Fragments arranged by size:
3244 915 492 262 246
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5159
SphI G_CATG'C
Cuts at: 2212 2712 2212
Size: 500 4659
SspI AAT'ATT
Cuts at: 861 861
Size: 5159
StyI C'CwwG_G
Cuts at: 320 660 1385 1430 1925 2114 2293 2647 320
Size: 340 725 45 495 189 179 354 2832
Fragments arranged by size:
2832 725 495 354 340 189 179 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 706 2742 2875 3334 3543 706
Size: 2036 133 459 209 2322
Fragments arranged by size:
2322 2036 459 209 133
TatI w'GTAC_w
Cuts at: 1763 4674 1763
Size: 2911 2248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 5127
Tth111I GACn'n_nGTC
Cuts at: 373 2956 373
Size: 2583 2576
XbaI T'CTAG_A
Cuts at: 2190 2190
Size: 5159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 1581 1848 2654 240
Size: 1341 267 806 2745
Fragments arranged by size:
2745 1341 806 267
XmaI C'CCGG_G
Cuts at: 1369 1446 1465 1533 2179 1369
Size: 77 19 68 646 4349
Fragments arranged by size:
4349 646 77 68 19
ZraI GAC'GTC
Cuts at: 339 339
Size: 5159
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AvrII BbsI BbvCI
BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI