Restriction map for
pF1KB9653
(Circular) (Six-base) MAPSORT of: pF1KB9653.seq Check: 6697 from: 1 to: 3941
pF1KB9653 3941 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 297 979 297
Size: 682 3259
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1766 2198 2945 3213 1766
Size: 432 747 268 2494
Fragments arranged by size:
2494 747 432 268
AfeI AGC'GCT
Cuts at: 229 229
Size: 3941
AflIII A'CryG_T
Cuts at: 546 680 1293 2397 3374 546
Size: 134 613 1104 977 1113
Fragments arranged by size:
1113 1104 977 613 134
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 945 3294 3550 945
Size: 2349 256 1336
Fragments arranged by size:
2349 1336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 508 792 801 844 961 508
Size: 284 9 43 117 3488
Fragments arranged by size:
3488 284 117 43 9
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 235 393 448 483 715 957 1371 1619 1654
Size: 158 55 35 232 242 414 248 35
Cuts at: 1654 235
Size: 2522
Fragments arranged by size:
2522 414 248 242 232 158 55 35
35
BanII G_rGCy'C
Cuts at: 171 637 955 1985 171
Size: 466 318 1030 2127
Fragments arranged by size:
2127 1030 466 318
BbeI G_GCGC'C
Cuts at: 239 452 719 1375 1623 239
Size: 213 267 656 248 2557
Fragments arranged by size:
2557 656 267 248 213
BbsI GAAGACnn'nnnn_
Cuts at: 543 543
Size: 3941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 635 669 415
Size: 34 186 34 3687
Fragments arranged by size:
3687 186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglI GCCn_nnn'nGGC
Cuts at: 410 410
Size: 3941
BglII A'GATC_T
Cuts at: 650 1456 3493 650
Size: 806 2037 1098
Fragments arranged by size:
2037 1098 806
BlpI GC'TnA_GC
Cuts at: 170 1053 170
Size: 883 3058
Bme1580I G_kGCm'C
Cuts at: 398 773 1566 1659 2715 398
Size: 375 793 93 1056 1624
Fragments arranged by size:
1624 1056 793 375 93
BmgBI CAC'GTC
Cuts at: 129 129
Size: 3941
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 424 1117 1996 2488 2786 352
Size: 72 693 879 492 298 1507
Fragments arranged by size:
1507 879 693 492 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 3941
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 236 422 449 716 1372 1620 2322 3818 236
Size: 186 27 267 656 248 702 1496 359
Fragments arranged by size:
1496 702 656 359 267 248 186 27
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 619
Size: 30 3911
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 355
Size: 3941
BseYI C'CCAG_C
Cuts at: 690 787 838 896 1366 2124 2701 3197 690
Size: 97 51 58 470 758 577 496 1434
Fragments arranged by size:
1434 758 577 496 470 97 58 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3941
BsiEI CG_ry'CG
Cuts at: 104 274 343 521 527 569 704 755 1483
Size: 170 69 178 6 42 135 51 728
Cuts at: 1483 1529 2737 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 728 178 170 135 69 51
46 42 6
BsiHKAI G_wGCw'C
Cuts at: 323 431 955 1733 1923 2715 323
Size: 108 524 778 190 792 1549
Fragments arranged by size:
1549 792 778 524 190 108
BsiWI C'GTAC_G
Cuts at: 677 677
Size: 3941
Bsp1286I G_dGCh'C
Cuts at: 171 323 398 431 637 773 955 1566 1659
Size: 152 75 33 206 136 182 611 93
Cuts at: 1659 1733 1923 1985 2715 171
Size: 74 190 62 730 1397
Fragments arranged by size:
1397 730 611 206 190 182 152 136
93 75 74 62 33
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 991 1205 1507 1888 991
Size: 214 302 381 3044
Fragments arranged by size:
3044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 861 2233 2287 3771 3936 50
Size: 811 1372 54 1484 165 55
Fragments arranged by size:
1484 1372 811 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 117 338 1939 2120 2329 117
Size: 221 1601 181 209 1729
Fragments arranged by size:
1729 1601 221 209 181
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 3941
BstYI r'GATC_y
Cuts at: 1 650 966 1433 1456 1791 2037 3049 3135
Size: 649 316 467 23 335 246 1012 86
Cuts at: 3135 3147 3493 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 316 246
86 23 12
BtgI C'CryG_G
Cuts at: 343 521 602 704 723 914 343
Size: 178 81 102 19 191 3370
Fragments arranged by size:
3370 191 178 102 81 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1184
Size: 3941
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 271 340 518 524 566 752 1526 1700 2091
Size: 69 178 6 42 186 774 174 391
Cuts at: 2091 2118 3463 271
Size: 27 1345 749
Fragments arranged by size:
1345 774 749 391 186 178 174 69
42 27 6
EagI C'GGCC_G
Cuts at: 271 340 518 524 566 752 1526 271
Size: 69 178 6 42 186 774 2686
Fragments arranged by size:
2686 774 186 178 69 42 6
EarI CTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 2149 2459 2605 3751 433
Size: 1716 310 146 1146 623
Fragments arranged by size:
1716 1146 623 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1766 2198 2945 3213 1766
Size: 432 747 268 2494
Fragments arranged by size:
2494 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 370 370
Size: 3941
EcoO109I rG'GnC_Cy
Cuts at: 1080 1080
Size: 3941
EcoRI G'AATT_C
Cuts at: 945 945
Size: 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 1960 402
Size: 1558 2383
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 231 239 452 719 1375 1623 2645 3624 231
Size: 8 213 267 656 248 1022 979 548
Fragments arranged by size:
1022 979 656 548 267 248 213 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 640 651 672 1783 1813 1815 1845 619
Size: 21 11 21 1111 30 2 30 2715
Fragments arranged by size:
2715 1111 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 235 448 715 1371 1619 235
Size: 213 267 656 248 2557
Fragments arranged by size:
2557 656 267 248 213
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 546 680 3374 546
Size: 134 2694 1113
Fragments arranged by size:
2694 1113 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 2612 2796 242
Size: 2370 184 1387
Fragments arranged by size:
2370 1387 184
MscI TGG'CCA
Cuts at: 1702 1702
Size: 3941
MslI CAynn'nnrTG
Cuts at: 310 1930 2057 310
Size: 1620 127 2194
Fragments arranged by size:
2194 1620 127
MspA1I CmG'CkG
Cuts at: 174 345 523 535 571 583 604 706 748
Size: 171 178 12 36 12 21 102 42
Cuts at: 748 900 916 1052 1366 1726 2739 2984 174
Size: 152 16 136 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 360 314 245 178 171 152
136 102 42 36 21 16 12 12
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 236 449 716 1372 1620 236
Size: 213 267 656 248 2557
Fragments arranged by size:
2557 656 267 248 213
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NotI GC'GGCC_GC
Cuts at: 518 524 752 518
Size: 6 228 3707
Fragments arranged by size:
3707 228 6
NspI r_CATG'y
Cuts at: 994 1494 2401 994
Size: 500 907 2534
Fragments arranged by size:
2534 907 500
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PstI C_TGCA'G
Cuts at: 747 829 854 988 1673 747
Size: 82 25 134 685 3015
Fragments arranged by size:
3015 685 134 82 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 900 1366 1726 900
Size: 466 360 3115
Fragments arranged by size:
3115 466 360
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SacII CC_GC'GG
Cuts at: 346 524 605 707 917 346
Size: 178 81 102 210 3370
Fragments arranged by size:
3370 210 178 102 81
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SfcI C'TryA_G
Cuts at: 33 743 825 850 984 1669 2662 2853 33
Size: 710 82 25 134 685 993 191 1121
Fragments arranged by size:
1121 993 710 685 191 134 82 25
SfoI GGC'GCC
Cuts at: 237 450 717 1373 1621 237
Size: 213 267 656 248 2557
Fragments arranged by size:
2557 656 267 248 213
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 794 803 846 963 794
Size: 9 43 117 3772
Fragments arranged by size:
3772 117 43 9
SmlI C'TyrA_G
Cuts at: 367 439 1096 2011 2503 2765 367
Size: 72 657 915 492 262 1543
Fragments arranged by size:
1543 915 657 492 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
SrfI GCCC'GGGC
Cuts at: 794 794
Size: 3941
StyI C'CwwG_G
Cuts at: 1075 1429 1075
Size: 354 3587
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 1657 2116 2325 1524
Size: 133 459 209 3140
Fragments arranged by size:
3140 459 209 133
TatI w'GTAC_w
Cuts at: 3456 3456
Size: 3941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 127 1738 127
Size: 1611 2330
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 792 801 844 961 792
Size: 9 43 117 3772
Fragments arranged by size:
3772 117 43 9
XmnI GAAnn'nnTTC
Cuts at: 148 148
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmtI BpmI Bpu10I
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI ZraI