Restriction map for
pF1KB9643
(Circular) (Six-base) MAPSORT of: pF1KB9643.seq Check: 4236 from: 1 to: 4334
pF1KB9643 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 352 1152 2159 2591 3338 3606 275
Size: 77 800 1007 432 747 268 1003
Fragments arranged by size:
1007 1003 800 747 432 268 77
AflIII A'CryG_T
Cuts at: 483 1686 2790 3767 483
Size: 1203 1104 977 1050
Fragments arranged by size:
1203 1104 1050 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AhdI GACnn_n'nnGTC
Cuts at: 563 563
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 298 630 1639 3206 298
Size: 332 1009 1567 1426
Fragments arranged by size:
1567 1426 1009 332
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 237 658 704 1269 1303 1338 3687 3943 237
Size: 421 46 565 34 35 2349 256 628
Fragments arranged by size:
2349 628 565 421 256 46 35 34
AseI AT'TA_AT
Cuts at: 20 305 20
Size: 285 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 1354 1354
Size: 4334
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 1350 1764 2012 2047 1350
Size: 414 248 35 3637
Fragments arranged by size:
3637 414 248 35
BanII G_rGCy'C
Cuts at: 1348 2378 1348
Size: 1030 3304
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 480 974 480
Size: 494 3840
BbvCI CC'TCA_GC
Cuts at: 815 815
Size: 4334
BciVI GTATCCnnnnn_n'
Cuts at: 585 2232 2993 4334 585
Size: 1647 761 1341 585
Fragments arranged by size:
1647 1341 761 585
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglII A'GATC_T
Cuts at: 126 365 1849 3886 126
Size: 239 1484 2037 574
Fragments arranged by size:
2037 1484 574 239
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 1959 2052 3108 1959
Size: 93 1056 3185
Fragments arranged by size:
3185 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1230 1546 1627 1962 4106 1230
Size: 316 81 335 2144 1458
Fragments arranged by size:
2144 1458 335 316 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 272 2701 272
Size: 2429 1905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1062
Size: 4334
Bpu10I CC'TnA_GC
Cuts at: 515 815 515
Size: 300 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1510 2389 2881 3179 992
Size: 518 879 492 298 2147
Fragments arranged by size:
2147 879 518 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 243 550 715 4038 243
Size: 307 165 3323 539
Fragments arranged by size:
3323 539 307 165
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 364 1848 3885 6
Size: 358 1484 2037 455
Fragments arranged by size:
2037 1484 455 358
BsaHI Gr'CG_yC
Cuts at: 564 1765 2013 2715 4211 564
Size: 1201 248 702 1496 687
Fragments arranged by size:
1496 1201 702 687 248
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1759 2517 3094 3590 1759
Size: 758 577 496 2503
Fragments arranged by size:
2503 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 476 1348 2126 2316 3108 476
Size: 872 778 190 792 1702
Fragments arranged by size:
1702 872 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 476 1348 1959 2052 2126 2316 2378 3108 476
Size: 872 611 93 74 190 62 730 1702
Fragments arranged by size:
1702 872 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 306 1384 1598 1900 2281 306
Size: 1078 214 302 381 2359
Fragments arranged by size:
2359 1078 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 809 2246 809
Size: 1437 2897
BsrFI r'CCGG_y
Cuts at: 2332 2513 2722 2332
Size: 181 209 3944
Fragments arranged by size:
3944 209 181
BssSI C'ACGA_G
Cuts at: 464 2605 2963 464
Size: 2141 358 1835
Fragments arranged by size:
2141 1835 358
BstAPI GCAn_nnn'nTGC
Cuts at: 203 861 1711 203
Size: 658 850 2826
Fragments arranged by size:
2826 850 658
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstYI r'GATC_y
Cuts at: 1 126 365 452 1359 1826 1849 2184 2430
Size: 125 239 87 907 467 23 335 246
Cuts at: 2430 3442 3528 3540 3886 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 907 467 449 346 335 246 239
125 87 86 23 12
BtgI C'CryG_G
Cuts at: 107 166 107
Size: 59 4275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 1577 1577
Size: 4334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 155 190 155
Size: 35 4299
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 1919 2093 2484 2511 3856 1919
Size: 174 391 27 1345 2397
Fragments arranged by size:
2397 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 547 2357 2567 547
Size: 1810 210 2314
Fragments arranged by size:
2314 1810 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 352 1062 1152 2159 2591 3338 3606 275
Size: 77 710 90 1007 432 747 268 1003
Fragments arranged by size:
1007 1003 747 710 432 268 90 77
EcoO109I rG'GnC_Cy
Cuts at: 1473 1473
Size: 4334
EcoRI G'AATT_C
Cuts at: 237 1338 237
Size: 1101 3233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1174 2353 1174
Size: 1179 3155
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 1768 2016 3038 4017 1768
Size: 248 1022 979 2085
Fragments arranged by size:
2085 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 549 581 866 898 2176 2206 2208 2238 549
Size: 32 285 32 1278 30 2 30 2645
Fragments arranged by size:
2645 1278 285 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3189 3005
Size: 184 4150
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 264 2323 2450 264
Size: 2059 127 2148
Fragments arranged by size:
2148 2059 127
MspA1I CmG'CkG
Cuts at: 441 1445 1759 2119 3132 3377 441
Size: 1004 314 360 1013 245 1398
Fragments arranged by size:
1398 1013 1004 360 314 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 107
Size: 4334
NdeI CA'TA_TG
Cuts at: 750 750
Size: 4334
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 137 278 487 1387 1887 2794 137
Size: 141 209 900 500 907 1677
Fragments arranged by size:
1677 907 900 500 209 141
PciI A'CATG_T
Cuts at: 483 2790 483
Size: 2307 2027
PflMI CCAn_nnn'nTGG
Cuts at: 962 962
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PsiI TTA'TAA
Cuts at: 1052 1052
Size: 4334
PstI C_TGCA'G
Cuts at: 317 1381 2066 317
Size: 1064 685 2585
Fragments arranged by size:
2585 1064 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 441 1759 2119 441
Size: 1318 360 2656
Fragments arranged by size:
2656 1318 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 317 1381 317
Size: 1064 3270
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 313 622 1377 2062 3055 3246 33
Size: 280 309 755 685 993 191 1121
Fragments arranged by size:
1121 993 755 685 309 280 191
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1007 1489 2404 2896 3158 1007
Size: 482 915 492 262 2183
Fragments arranged by size:
2183 915 492 482 262
SpeI A'CTAG_T
Cuts at: 67 604 978 67
Size: 537 374 3423
Fragments arranged by size:
3423 537 374
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StyI C'CwwG_G
Cuts at: 107 596 1468 1822 107
Size: 489 872 354 2619
Fragments arranged by size:
2619 872 489 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1917 2050 2509 2718 1917
Size: 133 459 209 3533
Fragments arranged by size:
3533 459 209 133
TatI w'GTAC_w
Cuts at: 3849 3849
Size: 4334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI