Restriction map for
pF1KB9640
(Circular) (Six-base) MAPSORT of: pF1KB9640.seq Check: 2733 from: 1 to: 4196
pF1KB9640 4196 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1234 1234
Size: 4196
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 2021 2453 3200 3468 871
Size: 1150 432 747 268 1599
Fragments arranged by size:
1599 1150 747 432 268
AflIII A'CryG_T
Cuts at: 405 503 782 1548 2652 3629 405
Size: 98 279 766 1104 977 972
Fragments arranged by size:
1104 977 972 766 279 98
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 395 395
Size: 4196
AlwNI CAG_nnn'CTG
Cuts at: 591 1501 3068 591
Size: 910 1567 1719
Fragments arranged by size:
1719 1567 910
ApaI G_GGCC'C
Cuts at: 304 304
Size: 4196
ApaLI G'TGCA_C
Cuts at: 2966 2966
Size: 4196
ApoI r'AATT_y
Cuts at: 1200 3549 3805 1200
Size: 2349 256 1591
Fragments arranged by size:
2349 1591 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 265 355 1216 265
Size: 90 861 3245
Fragments arranged by size:
3245 861 90
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 462 1212 1626 1874 1909 462
Size: 750 414 248 35 2749
Fragments arranged by size:
2749 750 414 248 35
BanII G_rGCy'C
Cuts at: 304 614 637 904 1210 2240 304
Size: 310 23 267 306 1030 2260
Fragments arranged by size:
2260 1030 310 306 267 23
BbeI G_GCGC'C
Cuts at: 1630 1878 1630
Size: 248 3948
BbsI GAAGACnn'nnnn_
Cuts at: 371 1101 371
Size: 730 3466
BciVI GTATCCnnnnn_n'
Cuts at: 2094 2855 4196 2094
Size: 761 1341 2094
Fragments arranged by size:
2094 1341 761
BclI T'GATC_A
Cuts at: 1716 1716
Size: 4196
BglI GCCn_nnn'nGGC
Cuts at: 451 460 451
Size: 9 4187
BglII A'GATC_T
Cuts at: 1028 1711 3748 1028
Size: 683 2037 1476
Fragments arranged by size:
2037 1476 683
BlpI GC'TnA_GC
Cuts at: 826 1308 826
Size: 482 3714
Bme1580I G_kGCm'C
Cuts at: 304 769 1821 1914 2970 304
Size: 465 1052 93 1056 1530
Fragments arranged by size:
1530 1056 1052 465 93
BmgBI CAC'GTC
Cuts at: 348 482 750 348
Size: 134 268 3794
Fragments arranged by size:
3794 268 134
BmrI ACTGGGnnnn_n'
Cuts at: 285 385 640 1408 1489 1824 3968 285
Size: 100 255 768 81 335 2144 513
Fragments arranged by size:
2144 768 513 335 255 100 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 2563 1050
Size: 1513 2683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 895 1006 1036 551
Size: 344 111 30 3711
Fragments arranged by size:
3711 344 111 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1372 2251 2743 3041 985
Size: 387 879 492 298 2140
Fragments arranged by size:
2140 879 492 387 298
BsaI GGTCTCn'nnnn_
Cuts at: 3900 3900
Size: 4196
BsaAI yAC'GTr
Cuts at: 408 1549 408
Size: 1141 3055
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 670 1627 1875 2577 4073 670
Size: 957 248 702 1496 793
Fragments arranged by size:
1496 957 793 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2858 3005 3416 1385
Size: 521 678 274 147 411 2165
Fragments arranged by size:
2165 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 549 579 549
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 892 1023 679
Size: 213 131 3852
Fragments arranged by size:
3852 213 131
BseYI C'CCAG_C
Cuts at: 247 762 1621 2379 2956 3452 247
Size: 515 859 758 577 496 991
Fragments arranged by size:
991 859 758 577 515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 577 911 566
Size: 11 334 3851
Fragments arranged by size:
3851 334 11
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 605 1210 1988 2178 2970 605
Size: 605 778 190 792 1831
Fragments arranged by size:
1831 792 778 605 190
BsmI GAATG_Cn'
Cuts at: 137 137
Size: 4196
BsmBI CGTCTCn'nnnn_
Cuts at: 661 982 661
Size: 321 3875
Bsp1286I G_dGCh'C
Cuts at: 304 605 614 637 769 904 1210 1821 1914
Size: 301 9 23 132 135 306 611 93
Cuts at: 1914 1988 2178 2240 2970 304
Size: 74 190 62 730 1530
Fragments arranged by size:
1530 730 611 306 301 190 135 132
93 74 62 23 9
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 797 1246 1460 1762 2143 377
Size: 420 449 214 302 381 2430
Fragments arranged by size:
2430 449 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 398 533 2488 2542 4026 4191 50
Size: 348 135 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 348 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 258 642 2108 258
Size: 384 1466 2346
Fragments arranged by size:
2346 1466 384
BsrFI r'CCGG_y
Cuts at: 2194 2375 2584 2194
Size: 181 209 3806
Fragments arranged by size:
3806 209 181
BssSI C'ACGA_G
Cuts at: 2467 2825 2467
Size: 358 3838
BstAPI GCAn_nnn'nTGC
Cuts at: 600 1573 600
Size: 973 3223
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstEII G'GTnAC_C
Cuts at: 279 279
Size: 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 333 333
Size: 4196
BstYI r'GATC_y
Cuts at: 1 1028 1221 1688 1711 2046 2292 3304 3390
Size: 1027 193 467 23 335 246 1012 86
Cuts at: 3390 3402 3748 1
Size: 12 346 449
Fragments arranged by size:
1027 1012 467 449 346 335 246 193
86 23 12
BtgI C'CryG_G
Cuts at: 107 304 107
Size: 197 3999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DraIII CAC_nnn'GTG
Cuts at: 279 600 1161 279
Size: 321 561 3314
Fragments arranged by size:
3314 561 321
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 110 320 489 1781 1955 2346 2373 3718 110
Size: 210 169 1292 174 391 27 1345 588
Fragments arranged by size:
1345 1292 588 391 210 174 169 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 898 1109 2404 2714 2860 4006 898
Size: 211 1295 310 146 1146 1088
Fragments arranged by size:
1295 1146 1088 310 211 146
EcoICRI GAG'CTC
Cuts at: 1208 1208
Size: 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 871 895 1006 1036 2021 2453 3200 3468
Size: 320 24 111 30 985 432 747 268
Cuts at: 3468 551
Size: 1279
Fragments arranged by size:
1279 985 747 432 320 268 111 30
24
EcoO109I rG'GnC_Cy
Cuts at: 300 1335 300
Size: 1035 3161
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 750 2215 750
Size: 1465 2731
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 763 1630 1878 2900 3879 763
Size: 867 248 1022 979 1080
Fragments arranged by size:
1080 1022 979 867 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 2038 2068 2070 2100 375
Size: 32 1631 30 2 30 2471
Fragments arranged by size:
2471 1631 32 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
KasI G'GCGC_C
Cuts at: 1626 1874 1626
Size: 248 3948
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 683 1037 2867 3051 668
Size: 15 354 1830 184 1813
Fragments arranged by size:
1830 1813 354 184 15
MscI TGG'CCA
Cuts at: 112 322 1957 112
Size: 210 1635 2351
Fragments arranged by size:
2351 1635 210
MslI CAynn'nnrTG
Cuts at: 964 2185 2312 964
Size: 1221 127 2848
Fragments arranged by size:
2848 1221 127
MspA1I CmG'CkG
Cuts at: 118 571 825 1307 1621 1981 2994 3239 118
Size: 453 254 482 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 482 453 360 314 254 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1627 1875 1627
Size: 248 3948
NcoI C'CATG_G
Cuts at: 107 304 107
Size: 197 3999
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NspI r_CATG'y
Cuts at: 183 540 1249 1749 2656 183
Size: 357 709 500 907 1723
Fragments arranged by size:
1723 907 709 500 357
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PflMI CCAn_nnn'nTGG
Cuts at: 446 446
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PspOMI G'GGCC_C
Cuts at: 300 300
Size: 4196
PstI C_TGCA'G
Cuts at: 808 1243 1928 808
Size: 435 685 3076
Fragments arranged by size:
3076 685 435
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 118 571 825 1621 1981 118
Size: 453 254 796 360 2333
Fragments arranged by size:
2333 796 453 360 254
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 1210 1210
Size: 4196
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 132 804 1239 1924 2917 3108 33
Size: 99 672 435 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 435 191 99
SfoI GGC'GCC
Cuts at: 1628 1876 1628
Size: 248 3948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 357 1218 357
Size: 861 3335
SmlI C'TyrA_G
Cuts at: 1000 1351 2266 2758 3020 1000
Size: 351 915 492 262 2176
Fragments arranged by size:
2176 915 492 351 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 183 540 1249 1749 183
Size: 357 709 500 2630
Fragments arranged by size:
2630 709 500 357
SrfI GCCC'GGGC
Cuts at: 357 357
Size: 4196
StuI AGG'CCT
Cuts at: 817 817
Size: 4196
StyI C'CwwG_G
Cuts at: 107 304 1330 1684 107
Size: 197 1026 354 2619
Fragments arranged by size:
2619 1026 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 367 1779 1912 2371 2580 267
Size: 100 1412 133 459 209 1883
Fragments arranged by size:
1883 1412 459 209 133 100
TatI w'GTAC_w
Cuts at: 506 3711 506
Size: 3205 991
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 549 581 3713 3745 549
Size: 32 3132 32 1000
Fragments arranged by size:
3132 1000 32 32
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1691 330
Size: 1361 2835
XmaI C'CCGG_G
Cuts at: 355 1216 355
Size: 861 3335
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI SwaI XhoI XmnI ZraI