Restriction map for
pF1KB9637
(Circular) (Six-base) MAPSORT of: pF1KB9637.seq Check: 4199 from: 1 to: 4346
pF1KB9637 4346 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1384 1384
Size: 4346
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 2171 2603 3350 3618 673
Size: 1498 432 747 268 1401
Fragments arranged by size:
1498 1401 747 432 268
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AleI CACnn'nnGTG
Cuts at: 140 349 140
Size: 209 4137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 582 783 1057 1651 3218 582
Size: 201 274 594 1567 1710
Fragments arranged by size:
1710 1567 594 274 201
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 1366 1366
Size: 4346
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 275 1362 1776 2024 2059 275
Size: 1087 414 248 35 2562
Fragments arranged by size:
2562 1087 414 248 35
BanII G_rGCy'C
Cuts at: 1360 2390 1360
Size: 1030 3316
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BbsI GAAGACnn'nnnn_
Cuts at: 163 571 163
Size: 408 3938
BbvCI CC'TCA_GC
Cuts at: 725 725
Size: 4346
BciVI GTATCCnnnnn_n'
Cuts at: 828 2244 3005 4346 828
Size: 1416 761 1341 828
Fragments arranged by size:
1416 1341 828 761
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BfrBI ATG'CAT
Cuts at: 1333 1333
Size: 4346
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 330 522 1243 1971 2064 3120 330
Size: 192 721 728 93 1056 1556
Fragments arranged by size:
1556 1056 728 721 192 93
BmrI ACTGGGnnnn_n'
Cuts at: 1558 1639 1974 4118 1558
Size: 81 335 2144 1786
Fragments arranged by size:
2144 1786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 352 178
Size: 174 4172
Bpu10I CC'TnA_GC
Cuts at: 725 1007 725
Size: 282 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 602 4050 602
Size: 3448 898
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 468 1777 2025 2727 4223 468
Size: 1309 248 702 1496 591
Fragments arranged by size:
1496 1309 702 591 248
BsaWI w'CCGG_w
Cuts at: 167 604 1535 2056 2734 3008 3155 3566 167
Size: 437 931 521 678 274 147 411 947
Fragments arranged by size:
947 931 678 521 437 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 105 135 105
Size: 30 4316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 821 247
Size: 574 3772
BseYI C'CCAG_C
Cuts at: 261 767 1771 2529 3106 3602 261
Size: 506 1004 758 577 496 1005
Fragments arranged by size:
1005 1004 758 577 506 496
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 147 346 622 1360 2138 2328 3120 147
Size: 199 276 738 778 190 792 1373
Fragments arranged by size:
1373 792 778 738 276 199 190
BsmI GAATG_Cn'
Cuts at: 1333 1333
Size: 4346
Bsp1286I G_dGCh'C
Cuts at: 147 330 346 522 622 1243 1360 1971 2064
Size: 183 16 176 100 621 117 611 93
Cuts at: 2064 2138 2328 2390 3120 147
Size: 74 190 62 730 1373
Fragments arranged by size:
1373 730 621 611 190 183 176 117
100 93 74 62 16
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1040 1083 1396 1610 1912 2293 1040
Size: 43 313 214 302 381 3093
Fragments arranged by size:
3093 381 313 302 214 43
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 783 1723 783
Size: 940 3406
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstXI CCAn_nnnn'nTGG
Cuts at: 216 529 216
Size: 313 4033
BstYI r'GATC_y
Cuts at: 1 1371 1838 1861 2196 2442 3454 3540 3552
Size: 1370 467 23 335 246 1012 86 12
Cuts at: 3552 3898 1
Size: 346 449
Fragments arranged by size:
1370 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1235 107
Size: 1128 3218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 306 1589 306
Size: 1283 3063
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DraIII CAC_nnn'GTG
Cuts at: 477 477
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 293 1931 2105 2496 2523 3868 293
Size: 1638 174 391 27 1345 771
Fragments arranged by size:
1638 1345 771 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 377 2369 2579 377
Size: 1992 210 2144
Fragments arranged by size:
2144 1992 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 824 2554 2864 3010 4156 824
Size: 1730 310 146 1146 1014
Fragments arranged by size:
1730 1146 1014 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 352 673 2171 2603 3350 3618 178
Size: 174 321 1498 432 747 268 906
Fragments arranged by size:
1498 906 747 432 321 268 174
EcoNI CCTnn'n_nnAGG
Cuts at: 1036 1036
Size: 4346
EcoO109I rG'GnC_Cy
Cuts at: 306 1168 1485 306
Size: 862 317 3167
Fragments arranged by size:
3167 862 317
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 1780 2028 3050 4029 1780
Size: 248 1022 979 2097
Fragments arranged by size:
2097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 955 987 2188 2218 2220 2250 955
Size: 32 1201 30 2 30 3051
Fragments arranged by size:
3051 1201 32 30 30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 672 3017 3201 672
Size: 2345 184 1817
Fragments arranged by size:
2345 1817 184
MscI TGG'CCA
Cuts at: 2107 2107
Size: 4346
MslI CAynn'nnrTG
Cuts at: 140 349 2335 2462 140
Size: 209 1986 127 2024
Fragments arranged by size:
2024 1986 209 127
MspA1I CmG'CkG
Cuts at: 724 783 1116 1294 1457 1771 2131 3144 3389
Size: 59 333 178 163 314 360 1013 245
Cuts at: 3389 724
Size: 1681
Fragments arranged by size:
1681 1013 360 333 314 245 178 163
59
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NcoI C'CATG_G
Cuts at: 107 1235 107
Size: 1128 3218
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NsiI A_TGCA'T
Cuts at: 1335 1335
Size: 4346
NspI r_CATG'y
Cuts at: 1399 1899 2806 1399
Size: 500 907 2939
Fragments arranged by size:
2939 907 500
PasI CC'CwG_GG
Cuts at: 1164 1164
Size: 4346
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 477 477
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 145 177 3485 3517 145
Size: 32 3308 32 974
Fragments arranged by size:
3308 974 32 32
PpuMI rG'GwC_Cy
Cuts at: 306 306
Size: 4346
PstI C_TGCA'G
Cuts at: 1053 1393 2078 1053
Size: 340 685 3321
Fragments arranged by size:
3321 685 340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 724 1116 1294 1771 2131 724
Size: 392 178 477 360 2939
Fragments arranged by size:
2939 477 392 360 178
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SexAI A'CCwGG_T
Cuts at: 473 473
Size: 4346
SfcI C'TryA_G
Cuts at: 33 1049 1389 2074 3067 3258 33
Size: 1016 340 685 993 191 1121
Fragments arranged by size:
1121 1016 993 685 340 191
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1501 2416 2908 3170 1501
Size: 915 492 262 2677
Fragments arranged by size:
2677 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StyI C'CwwG_G
Cuts at: 107 404 1037 1235 1480 1834 107
Size: 297 633 198 245 354 2619
Fragments arranged by size:
2619 633 354 297 245 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 215 1929 2062 2521 2730 215
Size: 1714 133 459 209 1831
Fragments arranged by size:
1831 1714 459 209 133
TatI w'GTAC_w
Cuts at: 248 857 3861 248
Size: 609 3004 733
Fragments arranged by size:
3004 733 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 732 764 3863 3895 490
Size: 32 210 32 3099 32 941
Fragments arranged by size:
3099 941 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1141 1841 1141
Size: 700 3646
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 160 160
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI