Restriction map for
pF1KB9636
(Circular) (Six-base) MAPSORT of: pF1KB9636.seq Check: 7572 from: 1 to: 3929
pF1KB9636 3929 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1754 2186 2933 3201 385
Size: 1369 432 747 268 1113
Fragments arranged by size:
1369 1113 747 432 268
AflIII A'CryG_T
Cuts at: 1281 2385 3362 1281
Size: 1104 977 1848
Fragments arranged by size:
1848 1104 977
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 409 409
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 1234 2801 1234
Size: 1567 2362
ApaLI G'TGCA_C
Cuts at: 640 649 2699 640
Size: 9 2050 1870
Fragments arranged by size:
2050 1870 9
ApoI r'AATT_y
Cuts at: 111 933 3282 3538 111
Size: 822 2349 256 502
Fragments arranged by size:
2349 822 502 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 262 949 262
Size: 687 3242
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 286 727 759 945 1359 1607 1642 286
Size: 441 32 186 414 248 35 2573
Fragments arranged by size:
2573 441 414 248 186 35 32
BanII G_rGCy'C
Cuts at: 127 943 1973 127
Size: 816 1030 2083
Fragments arranged by size:
2083 1030 816
BbeI G_GCGC'C
Cuts at: 290 763 1363 1611 290
Size: 473 600 248 2608
Fragments arranged by size:
2608 600 473 248
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 886 886
Size: 3929
BglII A'GATC_T
Cuts at: 557 1444 3481 557
Size: 887 2037 1005
Fragments arranged by size:
2037 1005 887
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 644 653 1554 1647 2703 644
Size: 9 901 93 1056 1870
Fragments arranged by size:
1870 1056 901 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 816 1141 1222 1557 3701 816
Size: 325 81 335 2144 1044
Fragments arranged by size:
2144 1044 335 325 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 550 117
Size: 433 3496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 904 1105 1984 2476 2774 739
Size: 165 201 879 492 298 1894
Fragments arranged by size:
1894 879 492 298 201 165
BsaI GGTCTCn'nnnn_
Cuts at: 231 3633 231
Size: 3402 527
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 287 760 1360 1608 2310 3806 287
Size: 473 600 248 702 1496 410
Fragments arranged by size:
1496 702 600 473 410 248
BsaWI w'CCGG_w
Cuts at: 150 1118 1639 2317 2591 2738 3149 150
Size: 968 521 678 274 147 411 930
Fragments arranged by size:
968 930 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 3929
BseYI C'CCAG_C
Cuts at: 786 1354 2112 2689 3185 786
Size: 568 758 577 496 1530
Fragments arranged by size:
1530 758 577 568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 611 902 1471 1517 2725 104
Size: 507 291 569 46 1208 1308
Fragments arranged by size:
1308 1208 569 507 291 46
BsiHKAI G_wGCw'C
Cuts at: 127 644 653 943 1721 1911 2703 127
Size: 517 9 290 778 190 792 1353
Fragments arranged by size:
1353 792 778 517 290 190 9
BsmBI CGTCTCn'nnnn_
Cuts at: 220 220
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 127 644 653 943 1554 1647 1721 1911 1973
Size: 517 9 290 611 93 74 190 62
Cuts at: 1973 2703 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 517 290 190 93 74
62 9
BspEI T'CCGG_A
Cuts at: 150 3149 150
Size: 2999 930
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 979 1193 1495 1876 979
Size: 214 302 381 3032
Fragments arranged by size:
3032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 868 2221 2275 3759 3924 50
Size: 818 1353 54 1484 165 55
Fragments arranged by size:
1484 1353 818 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 610 1927 2108 2317 610
Size: 1317 181 209 2222
Fragments arranged by size:
2222 1317 209 181
BssHII G'CGCG_C
Cuts at: 668 668
Size: 3929
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 258 648 836 1306 258
Size: 390 188 470 2881
Fragments arranged by size:
2881 470 390 188
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 528 528
Size: 3929
BstYI r'GATC_y
Cuts at: 1 557 954 1421 1444 1779 2025 3037 3123
Size: 556 397 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 556 467 449 397 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 172 172
Size: 3929
BtgI C'CryG_G
Cuts at: 296 413 596 296
Size: 117 183 3629
Fragments arranged by size:
3629 183 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 585 1172 585
Size: 587 3342
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DraIII CAC_nnn'GTG
Cuts at: 648 648
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 608 612 629 899 1514 1688 2079 2106 3451
Size: 4 17 270 615 174 391 27 1345
Cuts at: 3451 608
Size: 1086
Fragments arranged by size:
1345 1086 615 391 270 174 27 17
4
EagI C'GGCC_G
Cuts at: 608 899 1514 608
Size: 291 615 3023
Fragments arranged by size:
3023 615 291
EarI CTCTTCn'nnn_
Cuts at: 535 1952 2162 535
Size: 1417 210 2302
Fragments arranged by size:
2302 1417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 693 2137 2447 2593 3739 693
Size: 1444 310 146 1146 883
Fragments arranged by size:
1444 1146 883 310 146
EcoICRI GAG'CTC
Cuts at: 125 941 125
Size: 816 3113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 385 550 1754 2186 2933 3201 117
Size: 268 165 1204 432 747 268 845
Fragments arranged by size:
1204 845 747 432 268 268 165
EcoO109I rG'GnC_Cy
Cuts at: 345 1068 345
Size: 723 3206
EcoRI G'AATT_C
Cuts at: 111 933 111
Size: 822 3107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FseI GG_CCGG'CC
Cuts at: 614 614
Size: 3929
FspI TGC'GCA
Cuts at: 463 1710 463
Size: 1247 2682
HaeII r_GCGC'y
Cuts at: 290 763 1363 1611 2633 3612 290
Size: 473 600 248 1022 979 607
Fragments arranged by size:
1022 979 607 600 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 1771 1801 1803 1833 109
Size: 32 1630 30 2 30 2205
Fragments arranged by size:
2205 1630 32 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 286 759 1359 1607 286
Size: 473 600 248 2608
Fragments arranged by size:
2608 600 473 248
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 2600 2784 317
Size: 2283 184 1462
Fragments arranged by size:
2283 1462 184
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 511 1918 2045 511
Size: 1407 127 2395
Fragments arranged by size:
2395 1407 127
MspA1I CmG'CkG
Cuts at: 298 333 699 786 794 1040 1354 1714 2727
Size: 35 366 87 8 246 314 360 1013
Cuts at: 2727 2972 298
Size: 245 1255
Fragments arranged by size:
1255 1013 366 360 314 246 245 87
35 8
NaeI GCC'GGC
Cuts at: 612 2110 612
Size: 1498 2431
NarI GG'CG_CC
Cuts at: 287 760 1360 1608 287
Size: 473 600 248 2608
Fragments arranged by size:
2608 600 473 248
NcoI C'CATG_G
Cuts at: 413 413
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 610 2108 610
Size: 1498 2431
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PpuMI rG'GwC_Cy
Cuts at: 345 345
Size: 3929
PstI C_TGCA'G
Cuts at: 785 976 1661 785
Size: 191 685 3053
Fragments arranged by size:
3053 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 333 786 1354 1714 333
Size: 453 568 360 2548
Fragments arranged by size:
2548 568 453 360
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 127 943 127
Size: 816 3113
SacII CC_GC'GG
Cuts at: 299 299
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 349 781 972 1657 2650 2841 33
Size: 316 432 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 432 316 191 191
SfoI GGC'GCC
Cuts at: 288 761 1361 1609 288
Size: 473 600 248 2608
Fragments arranged by size:
2608 600 473 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 264 951 264
Size: 687 3242
SmlI C'TyrA_G
Cuts at: 754 919 1084 1999 2491 2753 754
Size: 165 165 915 492 262 1930
Fragments arranged by size:
1930 915 492 262 165 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 290 413 908 1063 1417 290
Size: 123 495 155 354 2802
Fragments arranged by size:
2802 495 354 155 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 213 1512 1645 2104 2313 213
Size: 1299 133 459 209 1829
Fragments arranged by size:
1829 1299 459 209 133
TatI w'GTAC_w
Cuts at: 314 3444 314
Size: 3130 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 808 882 1424 808
Size: 74 542 3313
Fragments arranged by size:
3313 542 74
XmaI C'CCGG_G
Cuts at: 262 949 262
Size: 687 3242
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI