Restriction map for
pF1KB9632
(Circular) (Six-base) MAPSORT of: pF1KB9632.seq Check: 1257 from: 1 to: 3755
pF1KB9632 3755 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1580 2012 2759 3027 1580
Size: 432 747 268 2308
Fragments arranged by size:
2308 747 432 268
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AhdI GACnn_n'nnGTC
Cuts at: 615 615
Size: 3755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 409 409
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaI G_GGCC'C
Cuts at: 279 279
Size: 3755
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 123 279 604 682 775 123
Size: 156 325 78 93 3103
Fragments arranged by size:
3103 325 156 93 78
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 564 771 1185 1433 1468 564
Size: 207 414 248 35 2851
Fragments arranged by size:
2851 414 248 207 35
BanII G_rGCy'C
Cuts at: 279 721 769 1799 279
Size: 442 48 1030 2235
Fragments arranged by size:
2235 1030 442 48
BbeI G_GCGC'C
Cuts at: 568 1189 1437 568
Size: 621 248 2886
Fragments arranged by size:
2886 621 248
BbsI GAAGACnn'nnnn_
Cuts at: 191 191
Size: 3755
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 279 297 1380 1473 2529 279
Size: 18 1083 93 1056 1505
Fragments arranged by size:
1505 1083 1056 93 18
BmgBI CAC'GTC
Cuts at: 206 206
Size: 3755
BmrI ACTGGGnnnn_n'
Cuts at: 621 625 967 1048 1383 3527 621
Size: 4 342 81 335 2144 849
Fragments arranged by size:
2144 849 342 335 81 4
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 691 574
Size: 117 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 931 1810 2302 2600 735
Size: 196 879 492 298 1890
Fragments arranged by size:
1890 879 492 298 196
BsaI GGTCTCn'nnnn_
Cuts at: 3459 3459
Size: 3755
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 112 419 565 1186 1434 2136 3632 112
Size: 307 146 621 248 702 1496 235
Fragments arranged by size:
1496 702 621 307 248 235 146
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 688
Size: 3755
BseYI C'CCAG_C
Cuts at: 217 582 690 1180 1938 2515 3011 217
Size: 365 108 490 758 577 496 961
Fragments arranged by size:
961 758 577 496 490 365 108
BsiEI CG_ry'CG
Cuts at: 104 378 1297 1343 2551 104
Size: 274 919 46 1208 1308
Fragments arranged by size:
1308 1208 919 274 46
BsiHKAI G_wGCw'C
Cuts at: 769 1547 1737 2529 769
Size: 778 190 792 1995
Fragments arranged by size:
1995 792 778 190
BsmI GAATG_Cn'
Cuts at: 398 398
Size: 3755
BsmBI CGTCTCn'nnnn_
Cuts at: 148 469 148
Size: 321 3434
Bsp1286I G_dGCh'C
Cuts at: 279 297 721 769 1380 1473 1547 1737 1799
Size: 18 424 48 611 93 74 190 62
Cuts at: 1799 2529 279
Size: 730 1505
Fragments arranged by size:
1505 730 611 424 190 93 74 62
48 18
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 805 1019 1321 1702 205
Size: 600 214 302 381 2258
Fragments arranged by size:
2258 600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 162 2047 2101 3585 3750 50
Size: 112 1885 54 1484 165 55
Fragments arranged by size:
1885 1484 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 314 561 1753 1934 2143 314
Size: 247 1192 181 209 1926
Fragments arranged by size:
1926 1192 247 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstYI r'GATC_y
Cuts at: 1 780 1247 1270 1605 1851 2863 2949 2961
Size: 779 467 23 335 246 1012 86 12
Cuts at: 2961 3307 1
Size: 346 449
Fragments arranged by size:
1012 779 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 570 998 570
Size: 428 3327
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 162 1461 2319 162
Size: 1299 858 1598
Fragments arranged by size:
1598 1299 858
EaeI y'GGCC_r
Cuts at: 1340 1514 1905 1932 3277 1340
Size: 174 391 27 1345 1818
Fragments arranged by size:
1818 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 251 708 1778 1988 251
Size: 457 1070 210 2018
Fragments arranged by size:
2018 1070 457 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 268 696 1963 2273 2419 3565 198
Size: 70 428 1267 310 146 1146 388
Fragments arranged by size:
1267 1146 428 388 310 146 70
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 691 1580 2012 2759 3027 574
Size: 117 889 432 747 268 1302
Fragments arranged by size:
1302 889 747 432 268 117
EcoO109I rG'GnC_Cy
Cuts at: 275 276 617 894 275
Size: 1 341 277 3136
Fragments arranged by size:
3136 341 277 1
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3755
FspI TGC'GCA
Cuts at: 246 526 1536 246
Size: 280 1010 2465
Fragments arranged by size:
2465 1010 280
HaeII r_GCGC'y
Cuts at: 511 568 1189 1437 2459 3438 511
Size: 57 621 248 1022 979 828
Fragments arranged by size:
1022 979 828 621 248 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 1597 1627 1629 1659 601
Size: 32 964 30 2 30 2697
Fragments arranged by size:
2697 964 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 564 1185 1433 564
Size: 621 248 2886
Fragments arranged by size:
2886 621 248
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 1516 1516
Size: 3755
MslI CAynn'nnrTG
Cuts at: 1744 1871 1744
Size: 127 3628
MspA1I CmG'CkG
Cuts at: 866 1180 1540 2553 2798 866
Size: 314 360 1013 245 1823
Fragments arranged by size:
1823 1013 360 314 245
NaeI GCC'GGC
Cuts at: 316 563 1936 316
Size: 247 1373 2135
Fragments arranged by size:
2135 1373 247
NarI GG'CG_CC
Cuts at: 565 1186 1434 565
Size: 621 248 2886
Fragments arranged by size:
2886 621 248
NgoMIV G'CCGG_C
Cuts at: 314 561 1934 314
Size: 247 1373 2135
Fragments arranged by size:
2135 1373 247
NspI r_CATG'y
Cuts at: 808 1308 2215 808
Size: 500 907 2348
Fragments arranged by size:
2348 907 500
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PpuMI rG'GwC_Cy
Cuts at: 617 617
Size: 3755
PspOMI G'GGCC_C
Cuts at: 275 275
Size: 3755
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 1180 1540 1180
Size: 360 3395
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SanDI GG'GwC_CC
Cuts at: 617 617
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 798 1483 2476 2667 33
Size: 765 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 191
SfoI GGC'GCC
Cuts at: 566 1187 1435 566
Size: 621 248 2886
Fragments arranged by size:
2886 621 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SgrAI Cr'CCGG_yG
Cuts at: 561 561
Size: 3755
SmaI CCC'GGG
Cuts at: 606 684 777 606
Size: 78 93 3584
Fragments arranged by size:
3584 93 78
SmlI C'TyrA_G
Cuts at: 714 910 1825 2317 2579 714
Size: 196 915 492 262 1890
Fragments arranged by size:
1890 915 492 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StyI C'CwwG_G
Cuts at: 889 1243 889
Size: 354 3401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 353 1552 353
Size: 1199 2556
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 1250
Size: 3755
XmaI C'CCGG_G
Cuts at: 604 682 775 604
Size: 78 93 3584
Fragments arranged by size:
3584 93 78
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI