Restriction map for pF1KB9630
 (Circular) (Six-base) MAPSORT of: pF1KB9630.seq  Check: 9931  from: 1  to: 3824



pF1KB9630  3824 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AccI GT'mk_AC

Cuts at:    862     862 
   Size:       3824
 
Acc65I G'GTAC_C

Cuts at:    840     840 
   Size:       3824
 
AclI AA'CG_TT

Cuts at:   3664    3664 
   Size:       3824
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1649    2081    2828    3096    1649 
   Size:        432     747     268    2377

  Fragments arranged by size: 

                2377     747     432     268
 
AflIII A'CryG_T

Cuts at:   1176    2280    3257    1176 
   Size:       1104     977    1743

  Fragments arranged by size: 

                1743    1104     977
 
AgeI A'CCGG_T

Cuts at:   2212    2212 
   Size:       3824
 
AhdI GACnn_n'nnGTC

Cuts at:    657     657 
   Size:       3824
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    436     478     436 
   Size:         42    3782
 
AlwNI CAG_nnn'CTG

Cuts at:   1129    2696    1129 
   Size:       1567    2257
 
ApaLI G'TGCA_C

Cuts at:   2594    2594 
   Size:       3824
 
ApoI r'AATT_y

Cuts at:    828    3177    3433     828 
   Size:       2349     256    1219

  Fragments arranged by size: 

                2349    1219     256
 
AscI GG'CGCG_CC

Cuts at:    260     260 
   Size:       3824
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3824
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3824
 
AvaI C'yCGr_G

Cuts at:    275     587     844     275 
   Size:        312     257    3255

  Fragments arranged by size: 

                3255     312     257
 
BamHI G'GATC_C

Cuts at:    849     849 
   Size:       3824
 
BanI G'GyrC_C

Cuts at:    326     583     673     840    1254    1502    1537     326 
   Size:        257      90     167     414     248      35    2613

  Fragments arranged by size: 

                2613     414     257     248     167      90      35
 
BanII G_rGCy'C

Cuts at:    537     670     838    1868     537 
   Size:        133     168    1030    2493

  Fragments arranged by size: 

                2493    1030     168     133
 
BbeI G_GCGC'C

Cuts at:    330    1258    1506     330 
   Size:        928     248    2648

  Fragments arranged by size: 

                2648     928     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    203     731     203 
   Size:        528    3296
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3790
 
BciVI GTATCCnnnnn_n'

Cuts at:   1722    2483    3824    1722 
   Size:        761    1341    1722

  Fragments arranged by size: 

                1722    1341     761
 
BclI T'GATC_A

Cuts at:   1344    1344 
   Size:       3824
 
BglII A'GATC_T

Cuts at:   1339    3376    1339 
   Size:       2037    1787
 
BlpI GC'TnA_GC

Cuts at:    936     936 
   Size:       3824
 
Bme1580I G_kGCm'C

Cuts at:    300     588    1449    1542    2598     300 
   Size:        288     861      93    1056    1526

  Fragments arranged by size: 

                1526    1056     861     288      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    698    1036    1117    1452    3596     698 
   Size:        338      81     335    2144     926

  Fragments arranged by size: 

                2144     926     338     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2191    2191 
   Size:       3824
 
Bpu10I CC'TnA_GC

Cuts at:    669     669 
   Size:       3824
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    142    1000    1879    2371    2669     142 
   Size:        858     879     492     298    1297

  Fragments arranged by size: 

                1297     879     858     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    118    3528     118 
   Size:       3410     414
 
BsaAI yAC'GTr

Cuts at:   1177    1177 
   Size:       3824
 
BsaBI GATnn'nnATC

Cuts at:      6    1338    3375       6 
   Size:       1332    2037     455

  Fragments arranged by size: 

                2037    1332     455
 
BsaHI Gr'CG_yC

Cuts at:    280     327    1255    1503    2205    3701     280 
   Size:         47     928     248     702    1496     403

  Fragments arranged by size: 

                1496     928     702     403     248      47
 
BsaWI w'CCGG_w

Cuts at:   1013    1534    2212    2486    2633    3044    1013 
   Size:        521     678     274     147     411    1793

  Fragments arranged by size: 

                1793     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    711    1249    2007    2584    3080     711 
   Size:        538     758     577     496    1455

  Fragments arranged by size: 

                1455     758     577     538     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    349     349 
   Size:       3824
 
BsiEI CG_ry'CG

Cuts at:    104     336     405    1366    1412    2620     104 
   Size:        232      69     961      46    1208    1308

  Fragments arranged by size: 

                1308    1208     961     232      69      46
 
BsiHKAI G_wGCw'C

Cuts at:    838    1616    1806    2598     838 
   Size:        778     190     792    2064

  Fragments arranged by size: 

                2064     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    496     496 
   Size:       3824
 
Bsp1286I G_dGCh'C

Cuts at:    300     537     588     670     838    1449    1542    1616    1806 
   Size:        237      51      82     168     611      93      74     190

Cuts at:   1806    1868    2598     300 
   Size:         62     730    1526

  Fragments arranged by size: 

                1526     730     611     237     190     168      93      82
                  74      62      51
 
BspEI T'CCGG_A

Cuts at:   3044    3044 
   Size:       3824
 
BspHI T'CATG_A

Cuts at:   3000    3000 
   Size:       3824
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    136     874    1088    1390    1771     136 
   Size:        738     214     302     381    2189

  Fragments arranged by size: 

                2189     738     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     350     693    2116    2170    3654    3819      50 
   Size:        300     343    1423      54    1484     165      55

  Fragments arranged by size: 

                1484    1423     343     300     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1736    1736 
   Size:       3824
 
BsrFI r'CCGG_y

Cuts at:    246     383    1822    2003    2212     246 
   Size:        137    1439     181     209    1858

  Fragments arranged by size: 

                1858    1439     209     181     137
 
BssHII G'CGCG_C

Cuts at:    260     260 
   Size:       3824
 
BssSI C'ACGA_G

Cuts at:   2095    2453    2095 
   Size:        358    3466
 
BstAPI GCAn_nnn'nTGC

Cuts at:    372    1201     372 
   Size:        829    2995
 
BstBI TT'CG_AA

Cuts at:   2185    2185 
   Size:       3824
 
BstYI r'GATC_y

Cuts at:      1     849    1316    1339    1674    1920    2932    3018    3030 
   Size:        848     467      23     335     246    1012      86      12

Cuts at:   3030    3376       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     848     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    330     330 
   Size:       3824
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1774    1955    2118    1774 
   Size:        181     163    3480

  Fragments arranged by size: 

                3480     181     163
 
BtsI GCAGTG_nn'

Cuts at:    171     448     547     616    1067     171 
   Size:        277      99      69     451    2928

  Fragments arranged by size: 

                2928     451     277      99      69
 
DraI TTT'AAA

Cuts at:    823     823 
   Size:       3824
 
DrdI GACnn_nn'nnGTC

Cuts at:   1530    2388    1530 
   Size:        858    2966
 
EaeI y'GGCC_r

Cuts at:    333     539    1409    1583    1974    2001    3346     333 
   Size:        206     870     174     391      27    1345     811

  Fragments arranged by size: 

                1345     870     811     391     206     174      27
 
EagI C'GGCC_G

Cuts at:    333    1409     333 
   Size:       1076    2748
 
EarI CTCTTCn'nnn_

Cuts at:   1847    2057    1847 
   Size:        210    3614
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    574    2032    2342    2488    3634     574 
   Size:       1458     310     146    1146     764

  Fragments arranged by size: 

                1458    1146     764     310     146
 
EcoICRI GAG'CTC

Cuts at:    836     836 
   Size:       3824
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1649    2081    2828    3096    1649 
   Size:        432     747     268    2377

  Fragments arranged by size: 

                2377     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    963     963 
   Size:       3824
 
EcoRI G'AATT_C

Cuts at:    828     828 
   Size:       3824
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1843    1843 
   Size:       3824
 
FspI TGC'GCA

Cuts at:    376     454    1605     376 
   Size:         78    1151    2595

  Fragments arranged by size: 

                2595    1151      78
 
HaeII r_GCGC'y

Cuts at:    330     361     597    1258    1506    2528    3507     330 
   Size:         31     236     661     248    1022     979     647

  Fragments arranged by size: 

                1022     979     661     647     248     236      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    643     675    1666    1696    1698    1728     643 
   Size:         32     991      30       2      30    2739

  Fragments arranged by size: 

                2739     991      32      30      30       2
 
HincII GTy'rAC

Cuts at:    863     863 
   Size:       3824
 
KasI G'GCGC_C

Cuts at:    326    1254    1502     326 
   Size:        928     248    2648

  Fragments arranged by size: 

                2648     928     248
 
KpnI G_GTAC'C

Cuts at:    844     844 
   Size:       3824
 
MluI A'CGCG_T

Cuts at:   3257    3257 
   Size:       3824
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    161     237    2495    2679     161 
   Size:         76    2258     184    1306

  Fragments arranged by size: 

                2258    1306     184      76
 
MscI TGG'CCA

Cuts at:   1585    1585 
   Size:       3824
 
MslI CAynn'nnrTG

Cuts at:   1813    1940    1813 
   Size:        127    3697
 
MspA1I CmG'CkG

Cuts at:    161     332     935    1249    1609    2622    2867     161 
   Size:        171     603     314     360    1013     245    1118

  Fragments arranged by size: 

                1118    1013     603     360     314     245     171
 
NaeI GCC'GGC

Cuts at:    248     385    2005     248 
   Size:        137    1620    2067

  Fragments arranged by size: 

                2067    1620     137
 
NarI GG'CG_CC

Cuts at:    327    1255    1503     327 
   Size:        928     248    2648

  Fragments arranged by size: 

                2648     928     248
 
NgoMIV G'CCGG_C

Cuts at:    246     383    2003     246 
   Size:        137    1620    2067

  Fragments arranged by size: 

                2067    1620     137
 
NruI TCG'CGA

Cuts at:    407     407 
   Size:       3824
 
NspI r_CATG'y

Cuts at:    423     877    1377    2284     423 
   Size:        454     500     907    1963

  Fragments arranged by size: 

                1963     907     500     454
 
PciI A'CATG_T

Cuts at:   2280    2280 
   Size:       3824
 
PmeI GTTT'AAAC

Cuts at:    823     823 
   Size:       3824
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2963    2995    2963 
   Size:         32    3792
 
PshAI GACnn'nnGTC

Cuts at:    343     343 
   Size:       3824
 
PstI C_TGCA'G

Cuts at:    871    1556     871 
   Size:        685    3139
 
PvuI CG_AT'CG

Cuts at:    104     405     104 
   Size:        301    3523
 
PvuII CAG'CTG

Cuts at:   1249    1609    1249 
   Size:        360    3464
 
RsrII CG'GwC_CG

Cuts at:   2019    2019 
   Size:       3824
 
SacI G_AGCT'C

Cuts at:    838     838 
   Size:       3824
 
SacII CC_GC'GG

Cuts at:    333     333 
   Size:       3824
 
SalI G'TCGA_C

Cuts at:    861     861 
   Size:       3824
 
SapI GCTCTTCn'nnn_

Cuts at:   1847    2057    1847 
   Size:        210    3614
 
SbfI CC_TGCA'GG

Cuts at:    871     871 
   Size:       3824
 
ScaI AGT'ACT

Cuts at:   3341    3341 
   Size:       3824
 
SfcI C'TryA_G

Cuts at:     33     867    1552    2545    2736      33 
   Size:        834     685     993     191    1121

  Fragments arranged by size: 

                1121     993     834     685     191
 
SfoI GGC'GCC

Cuts at:    328    1256    1504     328 
   Size:        928     248    2648

  Fragments arranged by size: 

                2648     928     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3824
 
SmaI CCC'GGG

Cuts at:    277     589     846     277 
   Size:        312     257    3255

  Fragments arranged by size: 

                3255     312     257
 
SmlI C'TyrA_G

Cuts at:    121     979    1894    2386    2648     121 
   Size:        858     915     492     262    1297

  Fragments arranged by size: 

                1297     915     858     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3824
 
SphI G_CATG'C

Cuts at:    423     877    1377     423 
   Size:        454     500    2870

  Fragments arranged by size: 

                2870     500     454
 
StyI C'CwwG_G

Cuts at:    958    1312     958 
   Size:        354    3470
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1407    1540    1999    2208    1407 
   Size:        133     459     209    3023

  Fragments arranged by size: 

                3023     459     209     133
 
TatI w'GTAC_w

Cuts at:   3339    3339 
   Size:       3824
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3341    3373    3341 
   Size:         32    3792
 
Tth111I GACn'n_nGTC

Cuts at:   1621    1621 
   Size:       3824
 
XbaI T'CTAG_A

Cuts at:    855     855 
   Size:       3824
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1319    1319 
   Size:       3824
 
XmaI C'CCGG_G

Cuts at:    275     587     844     275 
   Size:        312     257    3255

  Fragments arranged by size: 

                3255     312     257
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NruI     NspI     PciI     PmeI     PpiI    PshAI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     ApaI    AvrII
     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI     BpmI    BsaXI
    BseRI    BsiWI     BsmI    BsrGI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI    PspXI
     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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