(Circular) (Six-base) MAPSORT of: pF1KB9630.seq Check: 9931 from: 1 to: 3824 pF1KB9630 3824 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 862 862 Size: 3824 Acc65I G'GTAC_C Cuts at: 840 840 Size: 3824 AclI AA'CG_TT Cuts at: 3664 3664 Size: 3824 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1649 2081 2828 3096 1649 Size: 432 747 268 2377 Fragments arranged by size: 2377 747 432 268 AflIII A'CryG_T Cuts at: 1176 2280 3257 1176 Size: 1104 977 1743 Fragments arranged by size: 1743 1104 977 AgeI A'CCGG_T Cuts at: 2212 2212 Size: 3824 AhdI GACnn_n'nnGTC Cuts at: 657 657 Size: 3824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 436 478 436 Size: 42 3782 AlwNI CAG_nnn'CTG Cuts at: 1129 2696 1129 Size: 1567 2257 ApaLI G'TGCA_C Cuts at: 2594 2594 Size: 3824 ApoI r'AATT_y Cuts at: 828 3177 3433 828 Size: 2349 256 1219 Fragments arranged by size: 2349 1219 256 AscI GG'CGCG_CC Cuts at: 260 260 Size: 3824 AseI AT'TA_AT Cuts at: 20 20 Size: 3824 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3824 AvaI C'yCGr_G Cuts at: 275 587 844 275 Size: 312 257 3255 Fragments arranged by size: 3255 312 257 BamHI G'GATC_C Cuts at: 849 849 Size: 3824 BanI G'GyrC_C Cuts at: 326 583 673 840 1254 1502 1537 326 Size: 257 90 167 414 248 35 2613 Fragments arranged by size: 2613 414 257 248 167 90 35 BanII G_rGCy'C Cuts at: 537 670 838 1868 537 Size: 133 168 1030 2493 Fragments arranged by size: 2493 1030 168 133 BbeI G_GCGC'C Cuts at: 330 1258 1506 330 Size: 928 248 2648 Fragments arranged by size: 2648 928 248 BbsI GAAGACnn'nnnn_ Cuts at: 203 731 203 Size: 528 3296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3790 BciVI GTATCCnnnnn_n' Cuts at: 1722 2483 3824 1722 Size: 761 1341 1722 Fragments arranged by size: 1722 1341 761 BclI T'GATC_A Cuts at: 1344 1344 Size: 3824 BglII A'GATC_T Cuts at: 1339 3376 1339 Size: 2037 1787 BlpI GC'TnA_GC Cuts at: 936 936 Size: 3824 Bme1580I G_kGCm'C Cuts at: 300 588 1449 1542 2598 300 Size: 288 861 93 1056 1526 Fragments arranged by size: 1526 1056 861 288 93 BmrI ACTGGGnnnn_n' Cuts at: 698 1036 1117 1452 3596 698 Size: 338 81 335 2144 926 Fragments arranged by size: 2144 926 338 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 3824 Bpu10I CC'TnA_GC Cuts at: 669 669 Size: 3824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 142 1000 1879 2371 2669 142 Size: 858 879 492 298 1297 Fragments arranged by size: 1297 879 858 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 118 3528 118 Size: 3410 414 BsaAI yAC'GTr Cuts at: 1177 1177 Size: 3824 BsaBI GATnn'nnATC Cuts at: 6 1338 3375 6 Size: 1332 2037 455 Fragments arranged by size: 2037 1332 455 BsaHI Gr'CG_yC Cuts at: 280 327 1255 1503 2205 3701 280 Size: 47 928 248 702 1496 403 Fragments arranged by size: 1496 928 702 403 248 47 BsaWI w'CCGG_w Cuts at: 1013 1534 2212 2486 2633 3044 1013 Size: 521 678 274 147 411 1793 Fragments arranged by size: 1793 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 711 1249 2007 2584 3080 711 Size: 538 758 577 496 1455 Fragments arranged by size: 1455 758 577 538 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 349 349 Size: 3824 BsiEI CG_ry'CG Cuts at: 104 336 405 1366 1412 2620 104 Size: 232 69 961 46 1208 1308 Fragments arranged by size: 1308 1208 961 232 69 46 BsiHKAI G_wGCw'C Cuts at: 838 1616 1806 2598 838 Size: 778 190 792 2064 Fragments arranged by size: 2064 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 496 496 Size: 3824 Bsp1286I G_dGCh'C Cuts at: 300 537 588 670 838 1449 1542 1616 1806 Size: 237 51 82 168 611 93 74 190 Cuts at: 1806 1868 2598 300 Size: 62 730 1526 Fragments arranged by size: 1526 730 611 237 190 168 93 82 74 62 51 BspEI T'CCGG_A Cuts at: 3044 3044 Size: 3824 BspHI T'CATG_A Cuts at: 3000 3000 Size: 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 136 874 1088 1390 1771 136 Size: 738 214 302 381 2189 Fragments arranged by size: 2189 738 381 302 214 BsrBI CCG'CTC Cuts at: 50 350 693 2116 2170 3654 3819 50 Size: 300 343 1423 54 1484 165 55 Fragments arranged by size: 1484 1423 343 300 165 55 54 BsrDI GCAATG_nn' Cuts at: 1736 1736 Size: 3824 BsrFI r'CCGG_y Cuts at: 246 383 1822 2003 2212 246 Size: 137 1439 181 209 1858 Fragments arranged by size: 1858 1439 209 181 137 BssHII G'CGCG_C Cuts at: 260 260 Size: 3824 BssSI C'ACGA_G Cuts at: 2095 2453 2095 Size: 358 3466 BstAPI GCAn_nnn'nTGC Cuts at: 372 1201 372 Size: 829 2995 BstBI TT'CG_AA Cuts at: 2185 2185 Size: 3824 BstYI r'GATC_y Cuts at: 1 849 1316 1339 1674 1920 2932 3018 3030 Size: 848 467 23 335 246 1012 86 12 Cuts at: 3030 3376 1 Size: 346 449 Fragments arranged by size: 1012 848 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 330 330 Size: 3824 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1774 1955 2118 1774 Size: 181 163 3480 Fragments arranged by size: 3480 181 163 BtsI GCAGTG_nn' Cuts at: 171 448 547 616 1067 171 Size: 277 99 69 451 2928 Fragments arranged by size: 2928 451 277 99 69 DraI TTT'AAA Cuts at: 823 823 Size: 3824 DrdI GACnn_nn'nnGTC Cuts at: 1530 2388 1530 Size: 858 2966 EaeI y'GGCC_r Cuts at: 333 539 1409 1583 1974 2001 3346 333 Size: 206 870 174 391 27 1345 811 Fragments arranged by size: 1345 870 811 391 206 174 27 EagI C'GGCC_G Cuts at: 333 1409 333 Size: 1076 2748 EarI CTCTTCn'nnn_ Cuts at: 1847 2057 1847 Size: 210 3614 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 574 2032 2342 2488 3634 574 Size: 1458 310 146 1146 764 Fragments arranged by size: 1458 1146 764 310 146 EcoICRI GAG'CTC Cuts at: 836 836 Size: 3824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1649 2081 2828 3096 1649 Size: 432 747 268 2377 Fragments arranged by size: 2377 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 963 963 Size: 3824 EcoRI G'AATT_C Cuts at: 828 828 Size: 3824 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1843 1843 Size: 3824 FspI TGC'GCA Cuts at: 376 454 1605 376 Size: 78 1151 2595 Fragments arranged by size: 2595 1151 78 HaeII r_GCGC'y Cuts at: 330 361 597 1258 1506 2528 3507 330 Size: 31 236 661 248 1022 979 647 Fragments arranged by size: 1022 979 661 647 248 236 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 643 675 1666 1696 1698 1728 643 Size: 32 991 30 2 30 2739 Fragments arranged by size: 2739 991 32 30 30 2 HincII GTy'rAC Cuts at: 863 863 Size: 3824 KasI G'GCGC_C Cuts at: 326 1254 1502 326 Size: 928 248 2648 Fragments arranged by size: 2648 928 248 KpnI G_GTAC'C Cuts at: 844 844 Size: 3824 MluI A'CGCG_T Cuts at: 3257 3257 Size: 3824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 161 237 2495 2679 161 Size: 76 2258 184 1306 Fragments arranged by size: 2258 1306 184 76 MscI TGG'CCA Cuts at: 1585 1585 Size: 3824 MslI CAynn'nnrTG Cuts at: 1813 1940 1813 Size: 127 3697 MspA1I CmG'CkG Cuts at: 161 332 935 1249 1609 2622 2867 161 Size: 171 603 314 360 1013 245 1118 Fragments arranged by size: 1118 1013 603 360 314 245 171 NaeI GCC'GGC Cuts at: 248 385 2005 248 Size: 137 1620 2067 Fragments arranged by size: 2067 1620 137 NarI GG'CG_CC Cuts at: 327 1255 1503 327 Size: 928 248 2648 Fragments arranged by size: 2648 928 248 NgoMIV G'CCGG_C Cuts at: 246 383 2003 246 Size: 137 1620 2067 Fragments arranged by size: 2067 1620 137 NruI TCG'CGA Cuts at: 407 407 Size: 3824 NspI r_CATG'y Cuts at: 423 877 1377 2284 423 Size: 454 500 907 1963 Fragments arranged by size: 1963 907 500 454 PciI A'CATG_T Cuts at: 2280 2280 Size: 3824 PmeI GTTT'AAAC Cuts at: 823 823 Size: 3824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2963 2995 2963 Size: 32 3792 PshAI GACnn'nnGTC Cuts at: 343 343 Size: 3824 PstI C_TGCA'G Cuts at: 871 1556 871 Size: 685 3139 PvuI CG_AT'CG Cuts at: 104 405 104 Size: 301 3523 PvuII CAG'CTG Cuts at: 1249 1609 1249 Size: 360 3464 RsrII CG'GwC_CG Cuts at: 2019 2019 Size: 3824 SacI G_AGCT'C Cuts at: 838 838 Size: 3824 SacII CC_GC'GG Cuts at: 333 333 Size: 3824 SalI G'TCGA_C Cuts at: 861 861 Size: 3824 SapI GCTCTTCn'nnn_ Cuts at: 1847 2057 1847 Size: 210 3614 SbfI CC_TGCA'GG Cuts at: 871 871 Size: 3824 ScaI AGT'ACT Cuts at: 3341 3341 Size: 3824 SfcI C'TryA_G Cuts at: 33 867 1552 2545 2736 33 Size: 834 685 993 191 1121 Fragments arranged by size: 1121 993 834 685 191 SfoI GGC'GCC Cuts at: 328 1256 1504 328 Size: 928 248 2648 Fragments arranged by size: 2648 928 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3824 SmaI CCC'GGG Cuts at: 277 589 846 277 Size: 312 257 3255 Fragments arranged by size: 3255 312 257 SmlI C'TyrA_G Cuts at: 121 979 1894 2386 2648 121 Size: 858 915 492 262 1297 Fragments arranged by size: 1297 915 858 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3824 SphI G_CATG'C Cuts at: 423 877 1377 423 Size: 454 500 2870 Fragments arranged by size: 2870 500 454 StyI C'CwwG_G Cuts at: 958 1312 958 Size: 354 3470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1407 1540 1999 2208 1407 Size: 133 459 209 3023 Fragments arranged by size: 3023 459 209 133 TatI w'GTAC_w Cuts at: 3339 3339 Size: 3824 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3341 3373 3341 Size: 32 3792 Tth111I GACn'n_nGTC Cuts at: 1621 1621 Size: 3824 XbaI T'CTAG_A Cuts at: 855 855 Size: 3824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1319 1319 Size: 3824 XmaI C'CCGG_G Cuts at: 275 587 844 275 Size: 312 257 3255 Fragments arranged by size: 3255 312 257 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI ApaI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI