Restriction map for
pF1KB9630
(Circular) (Six-base) MAPSORT of: pF1KB9630.seq Check: 9931 from: 1 to: 3824
pF1KB9630 3824 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1649 2081 2828 3096 1649
Size: 432 747 268 2377
Fragments arranged by size:
2377 747 432 268
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AhdI GACnn_n'nnGTC
Cuts at: 657 657
Size: 3824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 436 478 436
Size: 42 3782
AlwNI CAG_nnn'CTG
Cuts at: 1129 2696 1129
Size: 1567 2257
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 828 3177 3433 828
Size: 2349 256 1219
Fragments arranged by size:
2349 1219 256
AscI GG'CGCG_CC
Cuts at: 260 260
Size: 3824
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 275 587 844 275
Size: 312 257 3255
Fragments arranged by size:
3255 312 257
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 326 583 673 840 1254 1502 1537 326
Size: 257 90 167 414 248 35 2613
Fragments arranged by size:
2613 414 257 248 167 90 35
BanII G_rGCy'C
Cuts at: 537 670 838 1868 537
Size: 133 168 1030 2493
Fragments arranged by size:
2493 1030 168 133
BbeI G_GCGC'C
Cuts at: 330 1258 1506 330
Size: 928 248 2648
Fragments arranged by size:
2648 928 248
BbsI GAAGACnn'nnnn_
Cuts at: 203 731 203
Size: 528 3296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3790
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 936 936
Size: 3824
Bme1580I G_kGCm'C
Cuts at: 300 588 1449 1542 2598 300
Size: 288 861 93 1056 1526
Fragments arranged by size:
1526 1056 861 288 93
BmrI ACTGGGnnnn_n'
Cuts at: 698 1036 1117 1452 3596 698
Size: 338 81 335 2144 926
Fragments arranged by size:
2144 926 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
Bpu10I CC'TnA_GC
Cuts at: 669 669
Size: 3824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1000 1879 2371 2669 142
Size: 858 879 492 298 1297
Fragments arranged by size:
1297 879 858 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 3528 118
Size: 3410 414
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 280 327 1255 1503 2205 3701 280
Size: 47 928 248 702 1496 403
Fragments arranged by size:
1496 928 702 403 248 47
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2486 2633 3044 1013
Size: 521 678 274 147 411 1793
Fragments arranged by size:
1793 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 711 1249 2007 2584 3080 711
Size: 538 758 577 496 1455
Fragments arranged by size:
1455 758 577 538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3824
BsiEI CG_ry'CG
Cuts at: 104 336 405 1366 1412 2620 104
Size: 232 69 961 46 1208 1308
Fragments arranged by size:
1308 1208 961 232 69 46
BsiHKAI G_wGCw'C
Cuts at: 838 1616 1806 2598 838
Size: 778 190 792 2064
Fragments arranged by size:
2064 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 496 496
Size: 3824
Bsp1286I G_dGCh'C
Cuts at: 300 537 588 670 838 1449 1542 1616 1806
Size: 237 51 82 168 611 93 74 190
Cuts at: 1806 1868 2598 300
Size: 62 730 1526
Fragments arranged by size:
1526 730 611 237 190 168 93 82
74 62 51
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 874 1088 1390 1771 136
Size: 738 214 302 381 2189
Fragments arranged by size:
2189 738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 350 693 2116 2170 3654 3819 50
Size: 300 343 1423 54 1484 165 55
Fragments arranged by size:
1484 1423 343 300 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1736 1736
Size: 3824
BsrFI r'CCGG_y
Cuts at: 246 383 1822 2003 2212 246
Size: 137 1439 181 209 1858
Fragments arranged by size:
1858 1439 209 181 137
BssHII G'CGCG_C
Cuts at: 260 260
Size: 3824
BssSI C'ACGA_G
Cuts at: 2095 2453 2095
Size: 358 3466
BstAPI GCAn_nnn'nTGC
Cuts at: 372 1201 372
Size: 829 2995
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstYI r'GATC_y
Cuts at: 1 849 1316 1339 1674 1920 2932 3018 3030
Size: 848 467 23 335 246 1012 86 12
Cuts at: 3030 3376 1
Size: 346 449
Fragments arranged by size:
1012 848 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 330 330
Size: 3824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 171 448 547 616 1067 171
Size: 277 99 69 451 2928
Fragments arranged by size:
2928 451 277 99 69
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2388 1530
Size: 858 2966
EaeI y'GGCC_r
Cuts at: 333 539 1409 1583 1974 2001 3346 333
Size: 206 870 174 391 27 1345 811
Fragments arranged by size:
1345 870 811 391 206 174 27
EagI C'GGCC_G
Cuts at: 333 1409 333
Size: 1076 2748
EarI CTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 574 2032 2342 2488 3634 574
Size: 1458 310 146 1146 764
Fragments arranged by size:
1458 1146 764 310 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1649 2081 2828 3096 1649
Size: 432 747 268 2377
Fragments arranged by size:
2377 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 963 963
Size: 3824
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 376 454 1605 376
Size: 78 1151 2595
Fragments arranged by size:
2595 1151 78
HaeII r_GCGC'y
Cuts at: 330 361 597 1258 1506 2528 3507 330
Size: 31 236 661 248 1022 979 647
Fragments arranged by size:
1022 979 661 647 248 236 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 1666 1696 1698 1728 643
Size: 32 991 30 2 30 2739
Fragments arranged by size:
2739 991 32 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 326 1254 1502 326
Size: 928 248 2648
Fragments arranged by size:
2648 928 248
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 237 2495 2679 161
Size: 76 2258 184 1306
Fragments arranged by size:
2258 1306 184 76
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 1813 1940 1813
Size: 127 3697
MspA1I CmG'CkG
Cuts at: 161 332 935 1249 1609 2622 2867 161
Size: 171 603 314 360 1013 245 1118
Fragments arranged by size:
1118 1013 603 360 314 245 171
NaeI GCC'GGC
Cuts at: 248 385 2005 248
Size: 137 1620 2067
Fragments arranged by size:
2067 1620 137
NarI GG'CG_CC
Cuts at: 327 1255 1503 327
Size: 928 248 2648
Fragments arranged by size:
2648 928 248
NgoMIV G'CCGG_C
Cuts at: 246 383 2003 246
Size: 137 1620 2067
Fragments arranged by size:
2067 1620 137
NruI TCG'CGA
Cuts at: 407 407
Size: 3824
NspI r_CATG'y
Cuts at: 423 877 1377 2284 423
Size: 454 500 907 1963
Fragments arranged by size:
1963 907 500 454
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PshAI GACnn'nnGTC
Cuts at: 343 343
Size: 3824
PstI C_TGCA'G
Cuts at: 871 1556 871
Size: 685 3139
PvuI CG_AT'CG
Cuts at: 104 405 104
Size: 301 3523
PvuII CAG'CTG
Cuts at: 1249 1609 1249
Size: 360 3464
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SacII CC_GC'GG
Cuts at: 333 333
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 867 1552 2545 2736 33
Size: 834 685 993 191 1121
Fragments arranged by size:
1121 993 834 685 191
SfoI GGC'GCC
Cuts at: 328 1256 1504 328
Size: 928 248 2648
Fragments arranged by size:
2648 928 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 277 589 846 277
Size: 312 257 3255
Fragments arranged by size:
3255 312 257
SmlI C'TyrA_G
Cuts at: 121 979 1894 2386 2648 121
Size: 858 915 492 262 1297
Fragments arranged by size:
1297 915 858 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 423 877 1377 423
Size: 454 500 2870
Fragments arranged by size:
2870 500 454
StyI C'CwwG_G
Cuts at: 958 1312 958
Size: 354 3470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 1540 1999 2208 1407
Size: 133 459 209 3023
Fragments arranged by size:
3023 459 209 133
TatI w'GTAC_w
Cuts at: 3339 3339
Size: 3824
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1319 1319
Size: 3824
XmaI C'CCGG_G
Cuts at: 275 587 844 275
Size: 312 257 3255
Fragments arranged by size:
3255 312 257
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PciI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI