Restriction map for
pF1KB9628
(Circular) (Six-base) MAPSORT of: pF1KB9628.seq Check: 8448 from: 1 to: 4106
pF1KB9628 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1009 1122 1009
Size: 113 3993
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 548 575 1931 2363 3110 3378 272
Size: 276 27 1356 432 747 268 1000
Fragments arranged by size:
1356 1000 747 432 276 268 27
AflIII A'CryG_T
Cuts at: 1037 1458 2562 3539 1037
Size: 421 1104 977 1604
Fragments arranged by size:
1604 1104 977 421
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 1411 2978 1411
Size: 1567 2539
ApaI G_GGCC'C
Cuts at: 640 862 640
Size: 222 3884
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1009 1122 1536 1784 1819 1009
Size: 113 414 248 35 3296
Fragments arranged by size:
3296 414 248 113 35
BanII G_rGCy'C
Cuts at: 187 369 593 640 862 1120 2150 187
Size: 182 224 47 222 258 1030 2143
Fragments arranged by size:
2143 1030 258 224 222 182 47
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 1083 1083
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BfrBI ATG'CAT
Cuts at: 408 408
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 238 1218 238
Size: 980 3126
Bme1580I G_kGCm'C
Cuts at: 640 862 1731 1824 2880 640
Size: 222 869 93 1056 1866
Fragments arranged by size:
1866 1056 869 222 93
BmrI ACTGGGnnnn_n'
Cuts at: 643 1318 1399 1734 3878 643
Size: 675 81 335 2144 871
Fragments arranged by size:
2144 871 675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 676 556
Size: 120 3986
Bpu10I CC'TnA_GC
Cuts at: 877 975 877
Size: 98 4008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 763 1282 2161 2653 2951 373
Size: 390 519 879 492 298 1528
Fragments arranged by size:
1528 879 519 492 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 920 3810 920
Size: 2890 1216
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 422 1537 1785 2487 3983 422
Size: 1115 248 702 1496 545
Fragments arranged by size:
1496 1115 702 545 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 134 164 565 595 134
Size: 30 401 30 3645
Fragments arranged by size:
3645 401 30 30
BseYI C'CCAG_C
Cuts at: 1531 2289 2866 3362 1531
Size: 758 577 496 2275
Fragments arranged by size:
2275 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1898 2088 2880 1120
Size: 778 190 792 2346
Fragments arranged by size:
2346 792 778 190
BsmI GAATG_Cn'
Cuts at: 987 987
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 187 369 593 640 862 1120 1731 1824 1898
Size: 182 224 47 222 258 611 93 74
Cuts at: 1898 2088 2150 2880 187
Size: 190 62 730 1413
Fragments arranged by size:
1413 730 611 258 224 222 190 182
93 74 62 47
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 664 2377 2735 664
Size: 1713 358 2035
Fragments arranged by size:
2035 1713 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 138 138
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 3214 3300 3312
Size: 1130 467 23 335 246 1012 86 12
Cuts at: 3312 3658 1
Size: 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 436 436
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 500 543 1691 1865 2256 2283 3628 500
Size: 43 1148 174 391 27 1345 978
Fragments arranged by size:
1345 1148 978 391 174 43 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 203 248 278 366 2129 2339 203
Size: 45 30 88 1763 210 1970
Fragments arranged by size:
1970 1763 210 88 45 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 548 556 575 676 1931 2363 3110 3378
Size: 276 8 19 101 1255 432 747 268
Cuts at: 3378 272
Size: 1000
Fragments arranged by size:
1255 1000 747 432 276 268 101 19
8
EcoO109I rG'GnC_Cy
Cuts at: 319 637 858 1073 1245 319
Size: 318 221 215 172 3180
Fragments arranged by size:
3180 318 221 215 172
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 367 2125 367
Size: 1758 2348
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 303 330 335 362 474 506 658
Size: 32 137 27 5 27 112 32 152
Cuts at: 658 690 1948 1978 1980 2010 134
Size: 32 1258 30 2 30 2230
Fragments arranged by size:
2230 1258 152 137 112 32 32 32
30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 153 1145 153
Size: 992 3114
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1013 1126 1013
Size: 113 3993
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 654 2777 2961 654
Size: 2123 184 1799
Fragments arranged by size:
2123 1799 184
MscI TGG'CCA
Cuts at: 502 545 1867 502
Size: 43 1322 2741
Fragments arranged by size:
2741 1322 43
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NdeI CA'TA_TG
Cuts at: 755 755
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NsiI A_TGCA'T
Cuts at: 410 410
Size: 4106
NspI r_CATG'y
Cuts at: 985 1041 1159 1659 2566 985
Size: 56 118 500 907 2525
Fragments arranged by size:
2525 907 500 118 56
PciI A'CATG_T
Cuts at: 1037 2562 1037
Size: 1525 2581
PfoI T'CCnGG_A
Cuts at: 163 533 653 163
Size: 370 120 3616
Fragments arranged by size:
3616 370 120
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PpuMI rG'GwC_Cy
Cuts at: 319 1073 319
Size: 754 3352
PspOMI G'GGCC_C
Cuts at: 636 858 636
Size: 222 3884
PstI C_TGCA'G
Cuts at: 841 1153 1838 841
Size: 312 685 3109
Fragments arranged by size:
3109 685 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SanDI GG'GwC_CC
Cuts at: 319 1073 319
Size: 754 3352
SapI GCTCTTCn'nnn_
Cuts at: 248 2129 2339 248
Size: 1881 210 2015
Fragments arranged by size:
2015 1881 210
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SexAI A'CCwGG_T
Cuts at: 219 611 219
Size: 392 3714
SfcI C'TryA_G
Cuts at: 33 837 936 1031 1149 1834 2827 3018 33
Size: 804 99 95 118 685 993 191 1121
Fragments arranged by size:
1121 993 804 685 191 118 99 95
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 352 778 1261 2176 2668 2930 352
Size: 426 483 915 492 262 1528
Fragments arranged by size:
1528 915 492 483 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 131 1240 1594 131
Size: 1109 354 2643
Fragments arranged by size:
2643 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 841 3621 841
Size: 2780 1326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 3623 3655 565
Size: 32 3026 32 1016
Fragments arranged by size:
3026 1016 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 609 1066 1601 609
Size: 457 535 3114
Fragments arranged by size:
3114 535 457
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI