Restriction map for
pF1KB9627
(Circular) (Six-base) MAPSORT of: pF1KB9627.seq Check: 8860 from: 1 to: 4259
pF1KB9627 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 1253 4099 1253
Size: 2846 1413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2516 3263 3531 2084
Size: 432 747 268 2812
Fragments arranged by size:
2812 747 432 268
AfeI AGC'GCT
Cuts at: 460 460
Size: 4259
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AhdI GACnn_n'nnGTC
Cuts at: 663 663
Size: 4259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 523 565 523
Size: 42 4217
AlwNI CAG_nnn'CTG
Cuts at: 576 681 1564 3131 576
Size: 105 883 1567 1704
Fragments arranged by size:
1704 1567 883 105
ApaI G_GGCC'C
Cuts at: 425 1000 1219 425
Size: 575 219 3465
Fragments arranged by size:
3465 575 219
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1263 3612 3868 1263
Size: 2349 256 1654
Fragments arranged by size:
2349 1654 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 238 263 795 1279 238
Size: 25 532 484 3218
Fragments arranged by size:
3218 532 484 25
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 549 766 775 1275 1689 1937 1972 549
Size: 217 9 500 414 248 35 2836
Fragments arranged by size:
2836 500 414 248 217 35 9
BanII G_rGCy'C
Cuts at: 134 252 425 970 1000 1219 1235 1273 2303
Size: 118 173 545 30 219 16 38 1030
Cuts at: 2303 134
Size: 2090
Fragments arranged by size:
2090 1030 545 219 173 118 38 30
16
BbeI G_GCGC'C
Cuts at: 770 1693 1941 770
Size: 923 248 3088
Fragments arranged by size:
3088 923 248
BbsI GAAGACnn'nnnn_
Cuts at: 188 1128 188
Size: 940 3319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4225
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 268 316 848 881 268
Size: 48 532 33 3646
Fragments arranged by size:
3646 532 48 33
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1003 1371 1003
Size: 368 3891
Bme1580I G_kGCm'C
Cuts at: 155 425 554 1000 1219 1884 1977 3033 155
Size: 270 129 446 219 665 93 1056 1381
Fragments arranged by size:
1381 1056 665 446 270 219 129 93
BmgBI CAC'GTC
Cuts at: 150 150
Size: 4259
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1435 2314 2806 3104 1024
Size: 411 879 492 298 2179
Fragments arranged by size:
2179 879 492 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 3963 3963
Size: 4259
BsaAI yAC'GTr
Cuts at: 672 1612 672
Size: 940 3319
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 767 1163 1690 1938 2640 4136 767
Size: 396 527 248 702 1496 890
Fragments arranged by size:
1496 890 702 527 396 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 967 997 298
Size: 30 639 30 3560
Fragments arranged by size:
3560 639 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 367 370 373 382 385 1041 1243 210
Size: 157 3 3 9 3 656 202 3226
Fragments arranged by size:
3226 656 202 157 9 3 3 3
BseYI C'CCAG_C
Cuts at: 167 342 1684 2442 3019 3515 167
Size: 175 1342 758 577 496 911
Fragments arranged by size:
1342 911 758 577 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 694
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 783 869 1212 1801 1847 3055 104
Size: 679 86 343 589 46 1208 1308
Fragments arranged by size:
1308 1208 679 589 343 86 46
BsiHKAI G_wGCw'C
Cuts at: 838 1235 1273 2051 2241 3033 838
Size: 397 38 778 190 792 2064
Fragments arranged by size:
2064 792 778 397 190 38
BsmBI CGTCTCn'nnnn_
Cuts at: 583 925 583
Size: 342 3917
Bsp1286I G_dGCh'C
Cuts at: 134 155 252 425 554 838 970 1000 1219
Size: 21 97 173 129 284 132 30 219
Cuts at: 1219 1235 1273 1884 1977 2051 2241 2303 3033
Size: 16 38 611 93 74 190 62 730
Cuts at: 3033 134
Size: 1360
Fragments arranged by size:
1360 730 611 284 219 190 173 132
129 97 93 74 62 38 30 21
16
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 1309 1523 1825 2206 545
Size: 764 214 302 381 2598
Fragments arranged by size:
2598 764 381 302 214
BsrBI CCG'CTC
Cuts at: 50 405 1002 1014 2551 2605 4089 4254 50
Size: 355 597 12 1537 54 1484 165 55
Fragments arranged by size:
1537 1484 597 355 165 55 54 12
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 721 778 868 2257 2438 2647 721
Size: 57 90 1389 181 209 2333
Fragments arranged by size:
2333 1389 209 181 90 57
BssHII G'CGCG_C
Cuts at: 448 448
Size: 4259
BssSI C'ACGA_G
Cuts at: 1063 2530 2888 1063
Size: 1467 358 2434
Fragments arranged by size:
2434 1467 358
BstAPI GCAn_nnn'nTGC
Cuts at: 900 1636 900
Size: 736 3523
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 714 717 714
Size: 3 4256
BstYI r'GATC_y
Cuts at: 1 632 1284 1751 1774 2109 2355 3367 3453
Size: 631 652 467 23 335 246 1012 86
Cuts at: 3453 3465 3811 1
Size: 12 346 449
Fragments arranged by size:
1012 652 631 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 288 789 909 1118 1125 288
Size: 501 120 209 7 3422
Fragments arranged by size:
3422 501 209 120 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 1502 1502
Size: 4259
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 269 317 652 719 780 866 1844 2018 2409
Size: 48 335 67 61 86 978 174 391
Cuts at: 2409 2436 3781 269
Size: 27 1345 747
Fragments arranged by size:
1345 978 747 391 335 174 86 67
61 48 27
EagI C'GGCC_G
Cuts at: 780 866 1844 780
Size: 86 978 3195
Fragments arranged by size:
3195 978 86
EarI CTCTTCn'nnn_
Cuts at: 290 359 2282 2492 290
Size: 69 1923 210 2057
Fragments arranged by size:
2057 1923 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 409 2467 2777 2923 4069 409
Size: 2058 310 146 1146 599
Fragments arranged by size:
2058 1146 599 310 146
EcoICRI GAG'CTC
Cuts at: 1233 1271 1233
Size: 38 4221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2516 3263 3531 2084
Size: 432 747 268 2812
Fragments arranged by size:
2812 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 541 541
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 996 1215 1216 1398 996
Size: 219 1 182 3857
Fragments arranged by size:
3857 219 182 1
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 468 2278 468
Size: 1810 2449
FspI TGC'GCA
Cuts at: 541 2040 541
Size: 1499 2760
HaeII r_GCGC'y
Cuts at: 462 625 770 1102 1693 1941 2963 3942 462
Size: 163 145 332 591 248 1022 979 779
Fragments arranged by size:
1022 979 779 591 332 248 163 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 2101 2131 2133 2163 649
Size: 32 1420 30 2 30 2745
Fragments arranged by size:
2745 1420 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
HindIII A'AGCT_T
Cuts at: 466 466
Size: 4259
KasI G'GCGC_C
Cuts at: 766 1689 1937 766
Size: 923 248 3088
Fragments arranged by size:
3088 923 248
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2930 3114 2930
Size: 184 4075
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 2248 2375 2248
Size: 127 4132
MspA1I CmG'CkG
Cuts at: 167 1370 1684 2044 3057 3302 167
Size: 1203 314 360 1013 245 1124
Fragments arranged by size:
1203 1124 1013 360 314 245
NaeI GCC'GGC
Cuts at: 723 780 870 2440 723
Size: 57 90 1570 2542
Fragments arranged by size:
2542 1570 90 57
NarI GG'CG_CC
Cuts at: 767 1690 1938 767
Size: 923 248 3088
Fragments arranged by size:
3088 923 248
NgoMIV G'CCGG_C
Cuts at: 721 778 868 2438 721
Size: 57 90 1570 2542
Fragments arranged by size:
2542 1570 90 57
NspI r_CATG'y
Cuts at: 510 1312 1812 2719 510
Size: 802 500 907 2050
Fragments arranged by size:
2050 907 802 500
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PspOMI G'GGCC_C
Cuts at: 421 996 1215 421
Size: 575 219 3465
Fragments arranged by size:
3465 575 219
PstI C_TGCA'G
Cuts at: 734 1306 1991 734
Size: 572 685 3002
Fragments arranged by size:
3002 685 572
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 167 1684 2044 167
Size: 1517 360 2382
Fragments arranged by size:
2382 1517 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1235 1273 1235
Size: 38 4221
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 730 1302 1987 2980 3171 33
Size: 697 572 685 993 191 1121
Fragments arranged by size:
1121 993 697 685 572 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 316 316
Size: 4259
SfoI GGC'GCC
Cuts at: 768 1691 1939 768
Size: 923 248 3088
Fragments arranged by size:
3088 923 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 240 265 1281 240
Size: 25 1016 3218
Fragments arranged by size:
3218 1016 25
SmlI C'TyrA_G
Cuts at: 1039 1414 2329 2821 3083 1039
Size: 375 915 492 262 2215
Fragments arranged by size:
2215 915 492 375 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 510 1312 1812 510
Size: 802 500 2957
Fragments arranged by size:
2957 802 500
SrfI GCCC'GGGC
Cuts at: 240 265 240
Size: 25 4234
StyI C'CwwG_G
Cuts at: 443 1393 1747 443
Size: 950 354 2955
Fragments arranged by size:
2955 950 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1842 1975 2434 2643 1842
Size: 133 459 209 3458
Fragments arranged by size:
3458 459 209 133
TatI w'GTAC_w
Cuts at: 3774 3774
Size: 4259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 2056 2056
Size: 4259
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 711 714 1754 450
Size: 261 3 1040 2955
Fragments arranged by size:
2955 1040 261 3
XmaI C'CCGG_G
Cuts at: 238 263 1279 238
Size: 25 1016 3218
Fragments arranged by size:
3218 1016 25
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI BpmI Bpu10I BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI