Restriction map for
pF1KB9625
(Circular) (Six-base) MAPSORT of: pF1KB9625.seq Check: 6990 from: 1 to: 3512
pF1KB9625 3512 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 506 506
Size: 3512
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 528 528
Size: 3512
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1337 1769 2516 2784 159
Size: 1178 432 747 268 887
Fragments arranged by size:
1178 887 747 432 268
AflIII A'CryG_T
Cuts at: 864 1968 2945 864
Size: 1104 977 1431
Fragments arranged by size:
1431 1104 977
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 163 182 288 817 2384 163
Size: 19 106 529 1567 1291
Fragments arranged by size:
1567 1291 529 106 19
ApaI G_GGCC'C
Cuts at: 202 202
Size: 3512
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 516 2865 3121 516
Size: 2349 256 907
Fragments arranged by size:
2349 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 242 532 242
Size: 290 3222
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 208 308 314 528 942 1190 1225 208
Size: 100 6 214 414 248 35 2495
Fragments arranged by size:
2495 414 248 214 100 35 6
BanII G_rGCy'C
Cuts at: 202 526 1556 202
Size: 324 1030 2158
Fragments arranged by size:
2158 1030 324
BbeI G_GCGC'C
Cuts at: 312 318 946 1194 312
Size: 6 628 248 2630
Fragments arranged by size:
2630 628 248 6
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 1032 1032
Size: 3512
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 202 1137 1230 2286 202
Size: 935 93 1056 1428
Fragments arranged by size:
1428 1056 935 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1567 2059 2357 688
Size: 879 492 298 1843
Fragments arranged by size:
1843 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3216 3216
Size: 3512
BsaAI yAC'GTr
Cuts at: 495 865 495
Size: 370 3142
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 309 315 503 943 1191 1893 3389 309
Size: 6 188 440 248 702 1496 432
Fragments arranged by size:
1496 702 440 432 248 188 6
BsaWI w'CCGG_w
Cuts at: 701 1222 1900 2174 2321 2732 701
Size: 521 678 274 147 411 1481
Fragments arranged by size:
1481 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 3512
BseYI C'CCAG_C
Cuts at: 937 1695 2272 2768 937
Size: 758 577 496 1681
Fragments arranged by size:
1681 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 526 1304 1494 2286 526
Size: 778 190 792 1752
Fragments arranged by size:
1752 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 202 526 1137 1230 1304 1494 1556 2286 202
Size: 324 611 93 74 190 62 730 1428
Fragments arranged by size:
1428 730 611 324 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 287 562 776 1078 1459 149
Size: 138 275 214 302 381 2202
Fragments arranged by size:
2202 381 302 275 214 138
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 305 311 1510 1691 1900 305
Size: 6 1199 181 209 1917
Fragments arranged by size:
1917 1199 209 181 6
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 3512
BstYI r'GATC_y
Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718
Size: 536 467 23 335 246 1012 86 12
Cuts at: 2718 3064 1
Size: 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 128 128
Size: 3512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1462 1643 1806 1462
Size: 181 163 3168
Fragments arranged by size:
3168 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 267 464 1097 1271 1662 1689 3034 267
Size: 197 633 174 391 27 1345 745
Fragments arranged by size:
1345 745 633 391 197 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 524 524
Size: 3512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1337 1769 2516 2784 159
Size: 1178 432 747 268 887
Fragments arranged by size:
1178 887 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 199 651 199
Size: 452 3060
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1531 1531
Size: 3512
FspI TGC'GCA
Cuts at: 403 1293 403
Size: 890 2622
HaeII r_GCGC'y
Cuts at: 306 312 318 946 1194 2216 3195 306
Size: 6 6 628 248 1022 979 623
Fragments arranged by size:
1022 979 628 623 248 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 347 379 1354 1384 1386 1416 151
Size: 32 164 32 975 30 2 30 2247
Fragments arranged by size:
2247 975 164 32 32 30 30 2
HincII GTy'rAC
Cuts at: 551 551
Size: 3512
KasI G'GCGC_C
Cuts at: 308 314 942 1190 308
Size: 6 628 248 2630
Fragments arranged by size:
2630 628 248 6
KpnI G_GTAC'C
Cuts at: 532 532
Size: 3512
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 466 1273 466
Size: 807 2705
MslI CAynn'nnrTG
Cuts at: 367 1501 1628 367
Size: 1134 127 2251
Fragments arranged by size:
2251 1134 127
MspA1I CmG'CkG
Cuts at: 623 937 1297 2310 2555 623
Size: 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 360 314 245
NaeI GCC'GGC
Cuts at: 307 313 1693 307
Size: 6 1380 2126
Fragments arranged by size:
2126 1380 6
NarI GG'CG_CC
Cuts at: 309 315 943 1191 309
Size: 6 628 248 2630
Fragments arranged by size:
2630 628 248 6
NcoI C'CATG_G
Cuts at: 128 128
Size: 3512
NgoMIV G'CCGG_C
Cuts at: 305 311 1691 305
Size: 6 1380 2126
Fragments arranged by size:
2126 1380 6
NspI r_CATG'y
Cuts at: 565 1065 1972 565
Size: 500 907 2105
Fragments arranged by size:
2105 907 500
PciI A'CATG_T
Cuts at: 1968 1968
Size: 3512
PflMI CCAn_nnn'nTGG
Cuts at: 498 498
Size: 3512
PfoI T'CCnGG_A
Cuts at: 225 225
Size: 3512
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PmlI CAC'GTG
Cuts at: 495 495
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PspOMI G'GGCC_C
Cuts at: 198 198
Size: 3512
PstI C_TGCA'G
Cuts at: 284 559 1244 284
Size: 275 685 2552
Fragments arranged by size:
2552 685 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 937 1297 937
Size: 360 3152
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 526 526
Size: 3512
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 280 555 1240 2233 2424 33
Size: 247 275 685 993 191 1121
Fragments arranged by size:
1121 993 685 275 247 191
SfoI GGC'GCC
Cuts at: 310 316 944 1192 310
Size: 6 628 248 2630
Fragments arranged by size:
2630 628 248 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SgrAI Cr'CCGG_yG
Cuts at: 305 311 305
Size: 6 3506
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 667 1582 2074 2336 667
Size: 915 492 262 1843
Fragments arranged by size:
1843 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
StyI C'CwwG_G
Cuts at: 128 646 1000 128
Size: 518 354 2640
Fragments arranged by size:
2640 518 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 1095 1228 1687 1896 166
Size: 929 133 459 209 1782
Fragments arranged by size:
1782 929 459 209 133
TatI w'GTAC_w
Cuts at: 3027 3027
Size: 3512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 165 1309 165
Size: 1144 2368
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
ZraI GAC'GTC
Cuts at: 504 504
Size: 3512
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI