Restriction map for
pF1KB9623
(Circular) (Six-base) MAPSORT of: pF1KB9623.seq Check: 1386 from: 1 to: 3551
pF1KB9623 3551 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 589 589
Size: 3551
Acc65I G'GTAC_C
Cuts at: 567 567
Size: 3551
AclI AA'CG_TT
Cuts at: 274 3391 274
Size: 3117 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
AflIII A'CryG_T
Cuts at: 903 2007 2984 903
Size: 1104 977 1470
Fragments arranged by size:
1470 1104 977
AgeI A'CCGG_T
Cuts at: 1939 1939
Size: 3551
AlwNI CAG_nnn'CTG
Cuts at: 856 2423 856
Size: 1567 1984
ApaLI G'TGCA_C
Cuts at: 2321 2321
Size: 3551
ApoI r'AATT_y
Cuts at: 555 2904 3160 555
Size: 2349 256 946
Fragments arranged by size:
2349 946 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
AvaI C'yCGr_G
Cuts at: 301 571 301
Size: 270 3281
AvrII C'CTAG_G
Cuts at: 450 450
Size: 3551
BamHI G'GATC_C
Cuts at: 576 576
Size: 3551
BanI G'GyrC_C
Cuts at: 152 258 420 567 981 1229 1264 152
Size: 106 162 147 414 248 35 2439
Fragments arranged by size:
2439 414 248 162 147 106 35
BanII G_rGCy'C
Cuts at: 292 565 1595 292
Size: 273 1030 2248
Fragments arranged by size:
2248 1030 273
BbeI G_GCGC'C
Cuts at: 985 1233 985
Size: 248 3303
BbsI GAAGACnn'nnnn_
Cuts at: 518 518
Size: 3551
BciVI GTATCCnnnnn_n'
Cuts at: 1449 2210 3551 1449
Size: 761 1341 1449
Fragments arranged by size:
1449 1341 761
BclI T'GATC_A
Cuts at: 1071 1071
Size: 3551
BglII A'GATC_T
Cuts at: 1066 3103 1066
Size: 2037 1514
BlpI GC'TnA_GC
Cuts at: 663 663
Size: 3551
Bme1580I G_kGCm'C
Cuts at: 263 425 1176 1269 2325 263
Size: 162 751 93 1056 1489
Fragments arranged by size:
1489 1056 751 162 93
BmrI ACTGGGnnnn_n'
Cuts at: 763 844 1179 3323 763
Size: 81 335 2144 991
Fragments arranged by size:
2144 991 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3551
Bpu10I CC'TnA_GC
Cuts at: 163 163
Size: 3551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1606 2098 2396 727
Size: 879 492 298 1882
Fragments arranged by size:
1882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3255 3255
Size: 3551
BsaAI yAC'GTr
Cuts at: 904 904
Size: 3551
BsaBI GATnn'nnATC
Cuts at: 6 1065 3102 6
Size: 1059 2037 455
Fragments arranged by size:
2037 1059 455
BsaHI Gr'CG_yC
Cuts at: 322 362 982 1230 1932 3428 322
Size: 40 620 248 702 1496 445
Fragments arranged by size:
1496 702 620 445 248 40
BsaWI w'CCGG_w
Cuts at: 740 1261 1939 2213 2360 2771 740
Size: 521 678 274 147 411 1520
Fragments arranged by size:
1520 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 424 547 382
Size: 42 123 3386
Fragments arranged by size:
3386 123 42
BseYI C'CCAG_C
Cuts at: 497 976 1734 2311 2807 497
Size: 479 758 577 496 1241
Fragments arranged by size:
1241 758 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 334 118
Size: 216 3335
BsiEI CG_ry'CG
Cuts at: 104 1093 1139 2347 104
Size: 989 46 1208 1308
Fragments arranged by size:
1308 1208 989 46
BsiHKAI G_wGCw'C
Cuts at: 292 565 1343 1533 2325 292
Size: 273 778 190 792 1518
Fragments arranged by size:
1518 792 778 273 190
BsmI GAATG_Cn'
Cuts at: 376 376
Size: 3551
Bsp1286I G_dGCh'C
Cuts at: 263 292 425 565 1176 1269 1343 1533 1595
Size: 29 133 140 611 93 74 190 62
Cuts at: 1595 2325 263
Size: 730 1489
Fragments arranged by size:
1489 730 611 190 140 133 93 74
62 29
BspEI T'CCGG_A
Cuts at: 2771 2771
Size: 3551
BspHI T'CATG_A
Cuts at: 2727 2727
Size: 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 601 815 1117 1498 601
Size: 214 302 381 2654
Fragments arranged by size:
2654 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1843 1897 3381 3546 50
Size: 1793 54 1484 165 55
Fragments arranged by size:
1793 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 1463
Size: 3551
BsrFI r'CCGG_y
Cuts at: 1549 1730 1939 1549
Size: 181 209 3161
Fragments arranged by size:
3161 209 181
BssSI C'ACGA_G
Cuts at: 334 1822 2180 334
Size: 1488 358 1705
Fragments arranged by size:
1705 1488 358
BstAPI GCAn_nnn'nTGC
Cuts at: 928 928
Size: 3551
BstBI TT'CG_AA
Cuts at: 1912 1912
Size: 3551
BstXI CCAn_nnnn'nTGG
Cuts at: 372 372
Size: 3551
BstYI r'GATC_y
Cuts at: 1 576 1043 1066 1401 1647 2659 2745 2757
Size: 575 467 23 335 246 1012 86 12
Cuts at: 2757 3103 1
Size: 346 449
Fragments arranged by size:
1012 575 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1501 1682 1845 1501
Size: 181 163 3207
Fragments arranged by size:
3207 181 163
BtsI GCAGTG_nn'
Cuts at: 794 794
Size: 3551
DraI TTT'AAA
Cuts at: 550 550
Size: 3551
DrdI GACnn_nn'nnGTC
Cuts at: 1257 2115 1257
Size: 858 2693
EaeI y'GGCC_r
Cuts at: 1136 1310 1701 1728 3073 1136
Size: 174 391 27 1345 1614
Fragments arranged by size:
1614 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1136 1136
Size: 3551
EarI CTCTTCn'nnn_
Cuts at: 524 1574 1784 524
Size: 1050 210 2291
Fragments arranged by size:
2291 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 233 1759 2069 2215 3361 233
Size: 1526 310 146 1146 423
Fragments arranged by size:
1526 1146 423 310 146
EcoICRI GAG'CTC
Cuts at: 290 563 290
Size: 273 3278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 690 690
Size: 3551
EcoRI G'AATT_C
Cuts at: 555 555
Size: 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1570 1570
Size: 3551
FspI TGC'GCA
Cuts at: 1332 1332
Size: 3551
HaeII r_GCGC'y
Cuts at: 985 1233 2255 3234 985
Size: 248 1022 979 1302
Fragments arranged by size:
1302 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1393 1423 1425 1455 1393
Size: 30 2 30 3489
Fragments arranged by size:
3489 30 30 2
HincII GTy'rAC
Cuts at: 590 590
Size: 3551
KasI G'GCGC_C
Cuts at: 981 1229 981
Size: 248 3303
KpnI G_GTAC'C
Cuts at: 571 571
Size: 3551
MluI A'CGCG_T
Cuts at: 2984 2984
Size: 3551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2406 2222
Size: 184 3367
MscI TGG'CCA
Cuts at: 1312 1312
Size: 3551
MslI CAynn'nnrTG
Cuts at: 334 370 433 1540 1667 334
Size: 36 63 1107 127 2218
Fragments arranged by size:
2218 1107 127 63 36
MspA1I CmG'CkG
Cuts at: 195 662 976 1336 2349 2594 195
Size: 467 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 467 360 314 245
NaeI GCC'GGC
Cuts at: 1732 1732
Size: 3551
NarI GG'CG_CC
Cuts at: 982 1230 982
Size: 248 3303
NcoI C'CATG_G
Cuts at: 107 107
Size: 3551
NgoMIV G'CCGG_C
Cuts at: 1730 1730
Size: 3551
NspI r_CATG'y
Cuts at: 333 604 1104 2011 333
Size: 271 500 907 1873
Fragments arranged by size:
1873 907 500 271
PciI A'CATG_T
Cuts at: 2007 2007
Size: 3551
PflMI CCAn_nnn'nTGG
Cuts at: 186 186
Size: 3551
PmeI GTTT'AAAC
Cuts at: 550 550
Size: 3551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 3519
PstI C_TGCA'G
Cuts at: 598 1283 598
Size: 685 2866
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3551
PvuII CAG'CTG
Cuts at: 195 976 1336 195
Size: 781 360 2410
Fragments arranged by size:
2410 781 360
RsrII CG'GwC_CG
Cuts at: 1746 1746
Size: 3551
SacI G_AGCT'C
Cuts at: 292 565 292
Size: 273 3278
SalI G'TCGA_C
Cuts at: 588 588
Size: 3551
SapI GCTCTTCn'nnn_
Cuts at: 1574 1784 1574
Size: 210 3341
SbfI CC_TGCA'GG
Cuts at: 598 598
Size: 3551
ScaI AGT'ACT
Cuts at: 3068 3068
Size: 3551
SfcI C'TryA_G
Cuts at: 33 594 1279 2272 2463 33
Size: 561 685 993 191 1121
Fragments arranged by size:
1121 993 685 561 191
SfoI GGC'GCC
Cuts at: 983 1231 983
Size: 248 3303
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
SmaI CCC'GGG
Cuts at: 573 573
Size: 3551
SmlI C'TyrA_G
Cuts at: 706 1621 2113 2375 706
Size: 915 492 262 1882
Fragments arranged by size:
1882 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3551
SphI G_CATG'C
Cuts at: 604 1104 604
Size: 500 3051
StyI C'CwwG_G
Cuts at: 107 450 685 1039 107
Size: 343 235 354 2619
Fragments arranged by size:
2619 354 343 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 1134 1267 1726 1935 405
Size: 729 133 459 209 2021
Fragments arranged by size:
2021 729 459 209 133
TatI w'GTAC_w
Cuts at: 3066 3066
Size: 3551
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3519
Tth111I GACn'n_nGTC
Cuts at: 321 1348 321
Size: 1027 2524
XbaI T'CTAG_A
Cuts at: 582 582
Size: 3551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1046 1046
Size: 3551
XmaI C'CCGG_G
Cuts at: 571 571
Size: 3551
XmnI GAAnn'nnTTC
Cuts at: 349 349
Size: 3551
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI