Restriction map for
pF1KB9621
(Circular) (Six-base) MAPSORT of: pF1KB9621.seq Check: 8278 from: 1 to: 5165
pF1KB9621 5165 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 128 609 128
Size: 481 4684
AccI GT'mk_AC
Cuts at: 485 1715 2203 485
Size: 1230 488 3447
Fragments arranged by size:
3447 1230 488
Acc65I G'GTAC_C
Cuts at: 1903 2181 1903
Size: 278 4887
AclI AA'CG_TT
Cuts at: 5005 5005
Size: 5165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 941 2990 3422 4169 4437 941
Size: 2049 432 747 268 1669
Fragments arranged by size:
2049 1669 747 432 268
AflIII A'CryG_T
Cuts at: 2517 3621 4598 2517
Size: 1104 977 3084
Fragments arranged by size:
3084 1104 977
AgeI A'CCGG_T
Cuts at: 3553 3553
Size: 5165
AhdI GACnn_n'nnGTC
Cuts at: 465 2070 465
Size: 1605 3560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1376 1376
Size: 5165
AlwNI CAG_nnn'CTG
Cuts at: 537 774 846 1119 2470 4037 537
Size: 237 72 273 1351 1567 1665
Fragments arranged by size:
1665 1567 1351 273 237 72
ApaI G_GGCC'C
Cuts at: 1002 1002
Size: 5165
ApaLI G'TGCA_C
Cuts at: 3935 3935
Size: 5165
ApoI r'AATT_y
Cuts at: 2169 4518 4774 2169
Size: 2349 256 2560
Fragments arranged by size:
2560 2349 256
AseI AT'TA_AT
Cuts at: 20 1895 20
Size: 1875 3290
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
AvaI C'yCGr_G
Cuts at: 116 1194 2185 116
Size: 1078 991 3096
Fragments arranged by size:
3096 1078 991
BamHI G'GATC_C
Cuts at: 2190 2190
Size: 5165
BanI G'GyrC_C
Cuts at: 1041 1903 2181 2595 2843 2878 1041
Size: 862 278 414 248 35 3328
Fragments arranged by size:
3328 862 414 278 248 35
BanII G_rGCy'C
Cuts at: 1002 2179 3209 1002
Size: 1177 1030 2958
Fragments arranged by size:
2958 1177 1030
BbeI G_GCGC'C
Cuts at: 2599 2847 2599
Size: 248 4917
BbsI GAAGACnn'nnnn_
Cuts at: 128 882 1659 128
Size: 754 777 3634
Fragments arranged by size:
3634 777 754
BbvCI CC'TCA_GC
Cuts at: 1260 2126 1260
Size: 866 4299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5131
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3824 5165 3063
Size: 761 1341 3063
Fragments arranged by size:
3063 1341 761
BclI T'GATC_A
Cuts at: 2685 2685
Size: 5165
BglI GCCn_nnn'nGGC
Cuts at: 256 256
Size: 5165
BglII A'GATC_T
Cuts at: 753 2680 4717 753
Size: 1927 2037 1201
Fragments arranged by size:
2037 1927 1201
BlpI GC'TnA_GC
Cuts at: 715 1304 2277 715
Size: 589 973 3603
Fragments arranged by size:
3603 973 589
Bme1580I G_kGCm'C
Cuts at: 1002 2790 2883 3939 1002
Size: 1788 93 1056 2228
Fragments arranged by size:
2228 1788 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 678 869 1129 1696 2377 2458 2793 4937 678
Size: 191 260 567 681 81 335 2144 906
Fragments arranged by size:
2144 906 681 567 335 260 191 81
BmtI G_CTAG'C
Cuts at: 376 376
Size: 5165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2082 3532 2082
Size: 1450 3715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1469 868
Size: 601 4564
Bpu10I CC'TnA_GC
Cuts at: 1260 2126 1260
Size: 866 4299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 622 2341 3220 3712 4010 250
Size: 372 1719 879 492 298 1405
Fragments arranged by size:
1719 1405 879 492 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 1430 1765 4869 1430
Size: 335 3104 1726
Fragments arranged by size:
3104 1726 335
BsaAI yAC'GTr
Cuts at: 2518 2518
Size: 5165
BsaBI GATnn'nnATC
Cuts at: 6 1144 2679 4716 6
Size: 1138 1535 2037 455
Fragments arranged by size:
2037 1535 1138 455
BsaHI Gr'CG_yC
Cuts at: 125 319 461 606 650 2596 2844 3546 5042
Size: 194 142 145 44 1946 248 702 1496
Cuts at: 5042 125
Size: 248
Fragments arranged by size:
1946 1496 702 248 248 194 145 142
44
BsaWI w'CCGG_w
Cuts at: 2354 2875 3553 3827 3974 4385 2354
Size: 521 678 274 147 411 3134
Fragments arranged by size:
3134 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 532 562 532
Size: 30 5135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 754 1248 235
Size: 519 494 4152
Fragments arranged by size:
4152 519 494
BseYI C'CCAG_C
Cuts at: 767 1722 2590 3348 3925 4421 767
Size: 955 868 758 577 496 1511
Fragments arranged by size:
1511 955 868 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 609 859 550
Size: 59 250 4856
Fragments arranged by size:
4856 250 59
BsiEI CG_ry'CG
Cuts at: 104 2707 2753 3961 104
Size: 2603 46 1208 1308
Fragments arranged by size:
2603 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1956 2179 2957 3147 3939 1956
Size: 223 778 190 792 3182
Fragments arranged by size:
3182 792 778 223 190
BsmI GAATG_Cn'
Cuts at: 1477 1477
Size: 5165
Bsp1286I G_dGCh'C
Cuts at: 1002 1956 2179 2790 2883 2957 3147 3209 3939
Size: 954 223 611 93 74 190 62 730
Cuts at: 3939 1002
Size: 2228
Fragments arranged by size:
2228 954 730 611 223 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4385 4385
Size: 5165
BspHI T'CATG_A
Cuts at: 1706 1797 4341 1706
Size: 91 2544 2530
Fragments arranged by size:
2544 2530 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2215 2429 2731 3112 2215
Size: 214 302 381 4268
Fragments arranged by size:
4268 381 302 214
BsrBI CCG'CTC
Cuts at: 50 816 867 3457 3511 4995 5160 50
Size: 766 51 2590 54 1484 165 55
Fragments arranged by size:
2590 1484 766 165 55 54 51
BsrDI GCAATG_nn'
Cuts at: 3077 3077
Size: 5165
BsrFI r'CCGG_y
Cuts at: 3163 3344 3553 3163
Size: 181 209 4775
Fragments arranged by size:
4775 209 181
BsrGI T'GTAC_A
Cuts at: 1028 1028
Size: 5165
BssSI C'ACGA_G
Cuts at: 1955 3436 3794 1955
Size: 1481 358 3326
Fragments arranged by size:
3326 1481 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1592 2542 1592
Size: 950 4215
BstBI TT'CG_AA
Cuts at: 3526 3526
Size: 5165
BstEII G'GTnAC_C
Cuts at: 1018 1018
Size: 5165
BstXI CCAn_nnnn'nTGG
Cuts at: 312 1140 312
Size: 828 4337
BstYI r'GATC_y
Cuts at: 1 753 1167 2190 2657 2680 3015 3261 4273
Size: 752 414 1023 467 23 335 246 1012
Cuts at: 4273 4359 4371 4717 1
Size: 86 12 346 449
Fragments arranged by size:
1023 1012 752 467 449 414 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 156 156
Size: 5165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3115 3296 3459 3115
Size: 181 163 4821
Fragments arranged by size:
4821 181 163
BtsI GCAGTG_nn'
Cuts at: 847 2408 847
Size: 1561 3604
DraI TTT'AAA
Cuts at: 2164 2164
Size: 5165
DraIII CAC_nnn'GTG
Cuts at: 456 2020 456
Size: 1564 3601
DrdI GACnn_nn'nnGTC
Cuts at: 1702 2871 3729 1702
Size: 1169 858 3138
Fragments arranged by size:
3138 1169 858
EaeI y'GGCC_r
Cuts at: 2750 2924 3315 3342 4687 2750
Size: 174 391 27 1345 3228
Fragments arranged by size:
3228 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2750 2750
Size: 5165
EarI CTCTTCn'nnn_
Cuts at: 3188 3398 3188
Size: 210 4955
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1758 3373 3683 3829 4975 1758
Size: 1615 310 146 1146 1948
Fragments arranged by size:
1948 1615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2177 2177
Size: 5165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 941 1469 2990 3422 4169 4437 868
Size: 73 528 1521 432 747 268 1596
Fragments arranged by size:
1596 1521 747 528 432 268 73
EcoO109I rG'GnC_Cy
Cuts at: 871 998 999 2304 871
Size: 127 1 1305 3732
Fragments arranged by size:
3732 1305 127 1
EcoRI G'AATT_C
Cuts at: 2169 2169
Size: 5165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 5165
FspI TGC'GCA
Cuts at: 2946 2946
Size: 5165
HaeII r_GCGC'y
Cuts at: 537 2599 2847 3869 4848 537
Size: 2062 248 1022 979 854
Fragments arranged by size:
2062 1022 979 854 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1177 1209 1779 1811 2056 2088 3007
Size: 32 694 32 570 32 245 32 919
Cuts at: 3007 3037 3039 3069 451
Size: 30 2 30 2547
Fragments arranged by size:
2547 919 694 570 245 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 486 1575 2204 486
Size: 1089 629 3447
Fragments arranged by size:
3447 1089 629
HindIII A'AGCT_T
Cuts at: 667 667
Size: 5165
KasI G'GCGC_C
Cuts at: 2595 2843 2595
Size: 248 4917
KpnI G_GTAC'C
Cuts at: 1907 2185 1907
Size: 278 4887
MluI A'CGCG_T
Cuts at: 4598 4598
Size: 5165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 3836 4020 575
Size: 3261 184 1720
Fragments arranged by size:
3261 1720 184
MscI TGG'CCA
Cuts at: 2926 2926
Size: 5165
MslI CAynn'nnrTG
Cuts at: 310 1393 3154 3281 310
Size: 1083 1761 127 2194
Fragments arranged by size:
2194 1761 1083 127
MspA1I CmG'CkG
Cuts at: 329 379 564 771 1264 2276 2590 2950 3963
Size: 50 185 207 493 1012 314 360 1013
Cuts at: 3963 4208 329
Size: 245 1286
Fragments arranged by size:
1286 1013 1012 493 360 314 245 207
185 50
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 5165
NarI GG'CG_CC
Cuts at: 2596 2844 2596
Size: 248 4917
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 5165
NheI G'CTAG_C
Cuts at: 372 372
Size: 5165
NspI r_CATG'y
Cuts at: 405 420 558 1590 1664 2218 2718 3625 405
Size: 15 138 1032 74 554 500 907 1945
Fragments arranged by size:
1945 1032 907 554 500 138 74 15
PciI A'CATG_T
Cuts at: 3621 3621
Size: 5165
PflMI CCAn_nnn'nTGG
Cuts at: 774 1119 774
Size: 345 4820
PmeI GTTT'AAAC
Cuts at: 2164 2164
Size: 5165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 5133
PpuMI rG'GwC_Cy
Cuts at: 871 871
Size: 5165
PspOMI G'GGCC_C
Cuts at: 998 998
Size: 5165
PstI C_TGCA'G
Cuts at: 249 885 1007 1098 2212 2897 249
Size: 636 122 91 1114 685 2517
Fragments arranged by size:
2517 1114 685 636 122 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5165
PvuII CAG'CTG
Cuts at: 379 564 771 1264 2590 2950 379
Size: 185 207 493 1326 360 2594
Fragments arranged by size:
2594 1326 493 360 207 185
RsrII CG'GwC_CG
Cuts at: 3360 3360
Size: 5165
SacI G_AGCT'C
Cuts at: 2179 2179
Size: 5165
SalI G'TCGA_C
Cuts at: 484 2202 484
Size: 1718 3447
SanDI GG'GwC_CC
Cuts at: 871 871
Size: 5165
SapI GCTCTTCn'nnn_
Cuts at: 3188 3398 3188
Size: 210 4955
SbfI CC_TGCA'GG
Cuts at: 2212 2212
Size: 5165
ScaI AGT'ACT
Cuts at: 976 2031 4682 976
Size: 1055 2651 1459
Fragments arranged by size:
2651 1459 1055
SexAI A'CCwGG_T
Cuts at: 1980 1980
Size: 5165
SfcI C'TryA_G
Cuts at: 33 245 559 881 1003 1094 1476 2208 2893
Size: 212 314 322 122 91 382 732 685
Cuts at: 2893 3886 4077 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 732 685 382 322 314 212
191 122 91
SfoI GGC'GCC
Cuts at: 2597 2845 2597
Size: 248 4917
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
SmaI CCC'GGG
Cuts at: 118 2187 118
Size: 2069 3096
SmlI C'TyrA_G
Cuts at: 265 637 2320 3235 3727 3989 265
Size: 372 1683 915 492 262 1441
Fragments arranged by size:
1683 1441 915 492 372 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5165
SphI G_CATG'C
Cuts at: 1590 2218 2718 1590
Size: 628 500 4037
Fragments arranged by size:
4037 628 500
SspI AAT'ATT
Cuts at: 1755 1755
Size: 5165
StuI AGG'CCT
Cuts at: 1916 1916
Size: 5165
StyI C'CwwG_G
Cuts at: 1133 2299 2653 1133
Size: 1166 354 3645
Fragments arranged by size:
3645 1166 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 2100 2748 2881 3340 3549 1314
Size: 786 648 133 459 209 2930
Fragments arranged by size:
2930 786 648 459 209 133
TatI w'GTAC_w
Cuts at: 974 1028 2029 4680 974
Size: 54 1001 2651 1459
Fragments arranged by size:
2651 1459 1001 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1873 1905 4682 4714 1873
Size: 32 2777 32 2324
Fragments arranged by size:
2777 2324 32 32
Tth111I GACn'n_nGTC
Cuts at: 2962 2962
Size: 5165
XbaI T'CTAG_A
Cuts at: 2196 2196
Size: 5165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 2660 453
Size: 2207 2958
XmaI C'CCGG_G
Cuts at: 116 2185 116
Size: 2069 3096
XmnI GAAnn'nnTTC
Cuts at: 726 726
Size: 5165
ZraI GAC'GTC
Cuts at: 126 607 126
Size: 481 4684
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI SwaI XhoI