Restriction map for
pF1KB9620
(Circular) (Six-base) MAPSORT of: pF1KB9620.seq Check: 724 from: 1 to: 4154
pF1KB9620 4154 bp
With 182 enzymes: SgfI *
September 28, 2010 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 267 1056 267
Size: 789 3365
AccI GT'mk_AC
Cuts at: 686 1192 686
Size: 506 3648
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 1979 2411 3158 3426 415
Size: 1564 432 747 268 1143
Fragments arranged by size:
1564 1143 747 432 268
AfeI AGC'GCT
Cuts at: 290 290
Size: 4154
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 496 528 496
Size: 32 4122
AlwNI CAG_nnn'CTG
Cuts at: 1112 1122 1459 3026 1112
Size: 10 337 1567 2240
Fragments arranged by size:
2240 1567 337 10
ApaI G_GGCC'C
Cuts at: 614 614
Size: 4154
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 189 205 409 415 615 1174 189
Size: 16 204 6 200 559 3169
Fragments arranged by size:
3169 559 204 200 16 6
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 157 210 337 351 715 733 843 1137 1170
Size: 53 127 14 364 18 110 294 33
Cuts at: 1170 1584 1832 1867 157
Size: 414 248 35 2444
Fragments arranged by size:
2444 414 364 294 248 127 110 53
35 33 18 14
BanII G_rGCy'C
Cuts at: 416 469 614 634 874 1168 2198 416
Size: 53 145 20 240 294 1030 2372
Fragments arranged by size:
2372 1030 294 240 145 53 20
BbeI G_GCGC'C
Cuts at: 161 214 341 719 737 1588 1836 161
Size: 53 127 378 18 851 248 2479
Fragments arranged by size:
2479 851 378 248 127 53 18
BbsI GAAGACnn'nnnn_
Cuts at: 1015 1015
Size: 4154
BbvCI CC'TCA_GC
Cuts at: 1093 1093
Size: 4154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 927 961 1038 1072 927
Size: 34 77 34 4009
Fragments arranged by size:
4009 77 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglI GCCn_nnn'nGGC
Cuts at: 152 661 152
Size: 509 3645
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 139 262 1266 139
Size: 123 1004 3027
Fragments arranged by size:
3027 1004 123
Bme1580I G_kGCm'C
Cuts at: 614 622 770 848 1142 1779 1872 2928 614
Size: 8 148 78 294 637 93 1056 1840
Fragments arranged by size:
1840 1056 637 294 148 93 78 8
BmgBI CAC'GTC
Cuts at: 852 1101 852
Size: 249 3905
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1002 2521 1002
Size: 1519 2635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 997 559
Size: 438 3716
Bpu10I CC'TnA_GC
Cuts at: 697 1093 697
Size: 396 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 808 1000 1063 1330 2209 2701 2999 277
Size: 531 192 63 267 879 492 298 1432
Fragments arranged by size:
1432 879 531 492 298 267 192 63
BsaI GGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 4154
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 158 211 338 716 734 1039 1585 1833 2535
Size: 53 127 378 18 305 546 248 702
Cuts at: 2535 4031 158
Size: 1496 281
Fragments arranged by size:
1496 702 546 378 305 281 248 127
53 18
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 1024 1054 496
Size: 30 498 30 3596
Fragments arranged by size:
3596 498 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 517
Size: 4154
BseYI C'CCAG_C
Cuts at: 343 1127 1579 2337 2914 3410 343
Size: 784 452 758 577 496 1087
Fragments arranged by size:
1087 784 758 577 496 452
BsiEI CG_ry'CG
Cuts at: 104 224 230 305 581 668 746 1696 1742
Size: 120 6 75 276 87 78 950 46
Cuts at: 1742 2950 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 950 276 120 87 78 75
46 6
BsiHKAI G_wGCw'C
Cuts at: 416 469 1168 1946 2136 2928 416
Size: 53 699 778 190 792 1642
Fragments arranged by size:
1642 792 778 699 190 53
BsiWI C'GTAC_G
Cuts at: 1145 1145
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 416 469 614 622 634 770 848 874 1142
Size: 53 145 8 12 136 78 26 268
Cuts at: 1142 1168 1779 1872 1946 2136 2198 2928 416
Size: 26 611 93 74 190 62 730 1642
Fragments arranged by size:
1642 730 611 268 190 145 136 93
78 74 62 53 26 26 12 8
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 267 1056 1204 1418 1720 2101 267
Size: 789 148 214 302 381 2320
Fragments arranged by size:
2320 789 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 143 2446 2500 3984 4149 50
Size: 93 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 2066 2066
Size: 4154
BsrFI r'CCGG_y
Cuts at: 229 2152 2333 2542 229
Size: 1923 181 209 1841
Fragments arranged by size:
1923 1841 209 181
BssHII G'CGCG_C
Cuts at: 327 327
Size: 4154
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1531 1137
Size: 394 3760
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstEII G'GTnAC_C
Cuts at: 447 447
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 444 894 444
Size: 450 3704
BstYI r'GATC_y
Cuts at: 1 1179 1646 1669 2004 2250 3262 3348 3360
Size: 1178 467 23 335 246 1012 86 12
Cuts at: 3360 3706 1
Size: 346 449
Fragments arranged by size:
1178 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 224 626 1043 107
Size: 117 402 417 3218
Fragments arranged by size:
3218 417 402 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 930 2104 2285 2448 143
Size: 787 1174 181 163 1849
Fragments arranged by size:
1849 1174 787 181 163
BtsI GCAGTG_nn'
Cuts at: 1397 1397
Size: 4154
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DraIII CAC_nnn'GTG
Cuts at: 1137 1137
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 221 227 302 494 578 623 665 1739 1913
Size: 6 75 192 84 45 42 1074 174
Cuts at: 1913 2304 2331 3676 221
Size: 391 27 1345 699
Fragments arranged by size:
1345 1074 699 391 192 174 84 75
45 42 27 6
EagI C'GGCC_G
Cuts at: 221 227 302 578 665 1739 221
Size: 6 75 276 87 1074 2636
Fragments arranged by size:
2636 1074 276 87 75 6
EarI CTCTTCn'nnn_
Cuts at: 992 2177 2387 992
Size: 1185 210 2759
Fragments arranged by size:
2759 1185 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 934 2362 2672 2818 3964 934
Size: 1428 310 146 1146 1124
Fragments arranged by size:
1428 1146 1124 310 146
EcoICRI GAG'CTC
Cuts at: 414 467 1166 414
Size: 53 699 3402
Fragments arranged by size:
3402 699 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 559 997 1979 2411 3158 3426 415
Size: 144 438 982 432 747 268 1143
Fragments arranged by size:
1143 982 747 438 432 268 144
EcoO109I rG'GnC_Cy
Cuts at: 534 610 611 1293 534
Size: 76 1 682 3395
Fragments arranged by size:
3395 682 76 1
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 937 1935 937
Size: 998 3156
HaeII r_GCGC'y
Cuts at: 139 161 214 292 341 423 682 719 737
Size: 22 53 78 49 82 259 37 18
Cuts at: 737 1588 1836 2858 3837 139
Size: 851 248 1022 979 456
Fragments arranged by size:
1022 979 851 456 259 248 82 78
53 49 37 22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1024 1056 1996 2026 2028 2058 1024
Size: 32 940 30 2 30 3120
Fragments arranged by size:
3120 940 32 30 30 2
HincII GTy'rAC
Cuts at: 1104 1193 1104
Size: 89 4065
KasI G'GCGC_C
Cuts at: 157 210 337 715 733 1584 1832 157
Size: 53 127 378 18 851 248 2479
Fragments arranged by size:
2479 851 378 248 127 53 18
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 2825 3009 461
Size: 2364 184 1606
Fragments arranged by size:
2364 1606 184
MscI TGG'CCA
Cuts at: 1915 1915
Size: 4154
MslI CAynn'nnrTG
Cuts at: 892 2143 2270 892
Size: 1251 127 2776
Fragments arranged by size:
2776 1251 127
MspA1I CmG'CkG
Cuts at: 170 226 583 1109 1119 1265 1579 1939 2952
Size: 56 357 526 10 146 314 360 1013
Cuts at: 2952 3197 170
Size: 245 1127
Fragments arranged by size:
1127 1013 526 360 357 314 245 146
56 10
NaeI GCC'GGC
Cuts at: 231 2335 231
Size: 2104 2050
NarI GG'CG_CC
Cuts at: 158 211 338 716 734 1585 1833 158
Size: 53 127 378 18 851 248 2479
Fragments arranged by size:
2479 851 378 248 127 53 18
NcoI C'CATG_G
Cuts at: 107 107
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 229 2333 229
Size: 2104 2050
NotI GC'GGCC_GC
Cuts at: 302 578 665 302
Size: 276 87 3791
Fragments arranged by size:
3791 276 87
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 496 528 3293 3325 496
Size: 32 2765 32 1325
Fragments arranged by size:
2765 1325 32 32
PspOMI G'GGCC_C
Cuts at: 610 610
Size: 4154
PspXI vC'TCGA_Gb
Cuts at: 415 415
Size: 4154
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 1119 1579 1939 1119
Size: 460 360 3334
Fragments arranged by size:
3334 460 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 416 469 1168 416
Size: 53 699 3402
Fragments arranged by size:
3402 699 53
SacII CC_GC'GG
Cuts at: 227 227
Size: 4154
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SexAI A'CCwGG_T
Cuts at: 1133 1133
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1197 1882 2875 3066 33
Size: 1164 685 993 191 1121
Fragments arranged by size:
1164 1121 993 685 191
SfoI GGC'GCC
Cuts at: 159 212 339 717 735 1586 1834 159
Size: 53 127 378 18 851 248 2479
Fragments arranged by size:
2479 851 378 248 127 53 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 191 207 1176 191
Size: 16 969 3169
Fragments arranged by size:
3169 969 16
SmlI C'TyrA_G
Cuts at: 292 415 823 1015 1078 1309 2224 2716 2978
Size: 123 408 192 63 231 915 492 262
Cuts at: 2978 292
Size: 1468
Fragments arranged by size:
1468 915 492 408 262 231 192 123
63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 107 485 1288 1642 107
Size: 378 803 354 2619
Fragments arranged by size:
2619 803 378 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1737 1870 2329 2538 1737
Size: 133 459 209 3353
Fragments arranged by size:
3353 459 209 133
TatI w'GTAC_w
Cuts at: 3669 3669
Size: 4154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4122
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XhoI C'TCGA_G
Cuts at: 415 415
Size: 4154
XmaI C'CCGG_G
Cuts at: 189 205 1174 189
Size: 16 969 3169
Fragments arranged by size:
3169 969 16
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PmeI PpiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BfrBI
BmtI BsgI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI