Restriction map for
pF1KB9615
(Circular) (Six-base) MAPSORT of: pF1KB9615.seq Check: 2621 from: 1 to: 3905
pF1KB9615 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 300 584 300
Size: 284 3621
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 786 921 786
Size: 135 3770
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 544 1730 2162 2909 3177 419
Size: 125 1186 432 747 268 1147
Fragments arranged by size:
1186 1147 747 432 268 125
AfeI AGC'GCT
Cuts at: 565 565
Size: 3905
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 412 814 1210 2777 412
Size: 402 396 1567 1540
Fragments arranged by size:
1567 1540 402 396
ApaLI G'TGCA_C
Cuts at: 679 797 2675 679
Size: 118 1878 1909
Fragments arranged by size:
1909 1878 118
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 148 191 684 925 148
Size: 43 493 241 3128
Fragments arranged by size:
3128 493 241 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 778 811 778
Size: 33 3872
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 749 786 921 1335 1583 1618 749
Size: 37 135 414 248 35 3036
Fragments arranged by size:
3036 414 248 135 37 35
BanII G_rGCy'C
Cuts at: 299 427 919 1949 299
Size: 128 492 1030 2255
Fragments arranged by size:
2255 1030 492 128
BbeI G_GCGC'C
Cuts at: 753 1339 1587 753
Size: 586 248 3071
Fragments arranged by size:
3071 586 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 655 689 655
Size: 34 3871
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 372 1425 372
Size: 1053 2852
BglI GCCn_nnn'nGGC
Cuts at: 203 203
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 386 634 683 709 801 1530 1623 2679 386
Size: 248 49 26 92 729 93 1056 1612
Fragments arranged by size:
1612 1056 729 248 93 92 49 26
BmrI ACTGGGnnnn_n'
Cuts at: 325 1117 1198 1533 3677 325
Size: 792 81 335 2144 553
Fragments arranged by size:
2144 792 553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 552 514
Size: 38 3867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1081 1960 2452 2750 229
Size: 852 879 492 298 1384
Fragments arranged by size:
1384 879 852 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 399 3609 399
Size: 3210 695
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 750 1336 1584 2286 3782 750
Size: 586 248 702 1496 873
Fragments arranged by size:
1496 873 702 586 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 682
Size: 30 3875
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 170 219 349 847 883 886 170
Size: 49 130 498 36 3 3189
Fragments arranged by size:
3189 498 130 49 36 3
BseYI C'CCAG_C
Cuts at: 118 412 1330 2088 2665 3161 118
Size: 294 918 758 577 496 862
Fragments arranged by size:
918 862 758 577 496 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 334 328
Size: 6 3899
BsiEI CG_ry'CG
Cuts at: 104 899 1447 1493 2701 104
Size: 795 548 46 1208 1308
Fragments arranged by size:
1308 1208 795 548 46
BsiHKAI G_wGCw'C
Cuts at: 683 801 919 1697 1887 2679 683
Size: 118 118 778 190 792 1909
Fragments arranged by size:
1909 792 778 190 118 118
Bsp1286I G_dGCh'C
Cuts at: 299 386 427 634 683 709 801 919 1530
Size: 87 41 207 49 26 92 118 611
Cuts at: 1530 1623 1697 1887 1949 2679 299
Size: 93 74 190 62 730 1525
Fragments arranged by size:
1525 730 611 207 190 118 93 92
87 74 62 49 41 26
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 300 584 955 1169 1471 1852 300
Size: 284 371 214 302 381 2353
Fragments arranged by size:
2353 381 371 302 284 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 601 894 1903 2084 2293 601
Size: 293 1009 181 209 2213
Fragments arranged by size:
2213 1009 293 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 545 1282 545
Size: 737 3168
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 804 930 1397 1420 1755 2001 3013 3099
Size: 803 126 467 23 335 246 1012 86
Cuts at: 3099 3111 3457 1
Size: 12 346 449
Fragments arranged by size:
1012 803 467 449 346 335 246 126
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 419 419
Size: 3905
BtgI C'CryG_G
Cuts at: 107 779 823 107
Size: 672 44 3189
Fragments arranged by size:
3189 672 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 658 1855 2036 2199 658
Size: 1197 181 163 2364
Fragments arranged by size:
2364 1197 181 163
BtsI GCAGTG_nn'
Cuts at: 348 585 1148 348
Size: 237 563 3105
Fragments arranged by size:
3105 563 237
ClaI AT'CG_AT
Cuts at: 368 368
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 896 1490 1664 2055 2082 3427 896
Size: 594 174 391 27 1345 1374
Fragments arranged by size:
1374 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 896 1490 896
Size: 594 3311
EarI CTCTTCn'nnn_
Cuts at: 152 860 1928 2138 152
Size: 708 1068 210 1919
Fragments arranged by size:
1919 1068 708 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 514 544 552 1730 2162 2909 3177 419
Size: 95 30 8 1178 432 747 268 1147
Fragments arranged by size:
1178 1147 747 432 268 95 30 8
EcoO109I rG'GnC_Cy
Cuts at: 441 1044 441
Size: 603 3302
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 501 567 696 753 1339 1587 2609 3588 501
Size: 66 129 57 586 248 1022 979 818
Fragments arranged by size:
1022 979 818 586 248 129 66 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 796 828 1747 1777 1779 1809 357
Size: 32 407 32 919 30 2 30 2453
Fragments arranged by size:
2453 919 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 749 1335 1583 749
Size: 586 248 3071
Fragments arranged by size:
3071 586 248
KpnI G_GTAC'C
Cuts at: 790 925 790
Size: 135 3770
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 2576 2760 512
Size: 2064 184 1657
Fragments arranged by size:
2064 1657 184
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 1894 2021 1894
Size: 127 3778
MspA1I CmG'CkG
Cuts at: 409 620 627 1016 1330 1690 2703 2948 409
Size: 211 7 389 314 360 1013 245 1366
Fragments arranged by size:
1366 1013 389 360 314 245 211 7
NaeI GCC'GGC
Cuts at: 603 896 2086 603
Size: 293 1190 2422
Fragments arranged by size:
2422 1190 293
NarI GG'CG_CC
Cuts at: 750 1336 1584 750
Size: 586 248 3071
Fragments arranged by size:
3071 586 248
NcoI C'CATG_G
Cuts at: 107 779 107
Size: 672 3233
NgoMIV G'CCGG_C
Cuts at: 601 894 2084 601
Size: 293 1190 2422
Fragments arranged by size:
2422 1190 293
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 948 1633 2626 2817 33
Size: 915 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 203 203
Size: 3905
SfoI GGC'GCC
Cuts at: 751 1337 1585 751
Size: 586 248 3071
Fragments arranged by size:
3071 586 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 150 193 927 150
Size: 43 734 3128
Fragments arranged by size:
3128 734 43
SmlI C'TyrA_G
Cuts at: 244 1060 1975 2467 2729 244
Size: 816 915 492 262 1420
Fragments arranged by size:
1420 915 816 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
StuI AGG'CCT
Cuts at: 250 250
Size: 3905
StyI C'CwwG_G
Cuts at: 107 779 1039 1393 107
Size: 672 260 354 2619
Fragments arranged by size:
2619 672 354 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XmaI C'CCGG_G
Cuts at: 148 191 925 148
Size: 43 734 3128
Fragments arranged by size:
3128 734 43
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI