Restriction map for
pF1KB9614
(Circular) (Six-base) MAPSORT of: pF1KB9614.seq Check: 5418 from: 1 to: 4619
pF1KB9614 4619 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1635 1635
Size: 4619
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 792 956 1212 2444 2876 3623 3891 173
Size: 619 164 256 1232 432 747 268 901
Fragments arranged by size:
1232 901 747 619 432 268 256 164
AflIII A'CryG_T
Cuts at: 242 532 1971 3075 4052 242
Size: 290 1439 1104 977 809
Fragments arranged by size:
1439 1104 977 809 290
AgeI A'CCGG_T
Cuts at: 3007 3007
Size: 4619
AhdI GACnn_n'nnGTC
Cuts at: 1565 1565
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 1924 3491 1924
Size: 1567 3052
ApaLI G'TGCA_C
Cuts at: 3389 3389
Size: 4619
ApoI r'AATT_y
Cuts at: 146 187 674 758 816 926 1010 1094 1178
Size: 41 487 84 58 110 84 84 84
Cuts at: 1178 1262 1346 1430 1514 1623 3972 4228 146
Size: 84 84 84 84 109 2349 256 537
Fragments arranged by size:
2349 537 487 256 110 109 84 84
84 84 84 84 84 84 58 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1639 1639
Size: 4619
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 665 1635 2049 2297 2332 665
Size: 970 414 248 35 2952
Fragments arranged by size:
2952 970 414 248 35
BanII G_rGCy'C
Cuts at: 1576 1633 2663 1576
Size: 57 1030 3532
Fragments arranged by size:
3532 1030 57
BbeI G_GCGC'C
Cuts at: 2053 2301 2053
Size: 248 4371
BbsI GAAGACnn'nnnn_
Cuts at: 245 892 1564 245
Size: 647 672 3300
Fragments arranged by size:
3300 672 647
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 1731 1731
Size: 4619
Bme1580I G_kGCm'C
Cuts at: 2244 2337 3393 2244
Size: 93 1056 3470
Fragments arranged by size:
3470 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1831 1912 2247 4391 1831
Size: 81 335 2144 2059
Fragments arranged by size:
2144 2059 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 788 872 1040 1292 1376 1544 704
Size: 84 84 168 252 84 168 3779
Fragments arranged by size:
3779 252 168 168 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 987 987
Size: 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1795 2674 3166 3464 333
Size: 1462 879 492 298 1488
Fragments arranged by size:
1488 1462 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 768 4323 768
Size: 3555 1064
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 2133 4170 6
Size: 2127 2037 455
Fragments arranged by size:
2127 2037 455
BsaHI Gr'CG_yC
Cuts at: 2050 2298 3000 4496 2050
Size: 248 702 1496 2173
Fragments arranged by size:
2173 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1808 2329 3007 3281 3428 3839 1808
Size: 521 678 274 147 411 2588
Fragments arranged by size:
2588 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 287 1573 287
Size: 1286 3333
BseYI C'CCAG_C
Cuts at: 397 2044 2802 3379 3875 397
Size: 1647 758 577 496 1141
Fragments arranged by size:
1647 1141 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1576 1633 2411 2601 3393 1576
Size: 57 778 190 792 2802
Fragments arranged by size:
2802 792 778 190 57
BsmI GAATG_Cn'
Cuts at: 958 1294 1488 958
Size: 336 194 4089
Fragments arranged by size:
4089 336 194
Bsp1286I G_dGCh'C
Cuts at: 1576 1633 2244 2337 2411 2601 2663 3393 1576
Size: 57 611 93 74 190 62 730 2802
Fragments arranged by size:
2802 730 611 190 93 74 62 57
BspEI T'CCGG_A
Cuts at: 3839 3839
Size: 4619
BspHI T'CATG_A
Cuts at: 213 3795 213
Size: 3582 1037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 1883 2185 2566 1669
Size: 214 302 381 3722
Fragments arranged by size:
3722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 963 2531 963
Size: 1568 3051
BsrFI r'CCGG_y
Cuts at: 2617 2798 3007 2617
Size: 181 209 4229
Fragments arranged by size:
4229 209 181
BssSI C'ACGA_G
Cuts at: 2890 3248 2890
Size: 358 4261
BstAPI GCAn_nnn'nTGC
Cuts at: 1996 1996
Size: 4619
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1644 2111 2134 2469 2715 3727 3813 3825
Size: 1643 467 23 335 246 1012 86 12
Cuts at: 3825 4171 1
Size: 346 449
Fragments arranged by size:
1643 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 299 299
Size: 4619
BtgI C'CryG_G
Cuts at: 107 269 107
Size: 162 4457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 1862 1862
Size: 4619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 945 980 945
Size: 35 4584
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 462 1146 2204 2378 2769 2796 4141 462
Size: 684 1058 174 391 27 1345 940
Fragments arranged by size:
1345 1058 940 684 391 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 269 2642 2852 269
Size: 2373 210 2036
Fragments arranged by size:
2373 2036 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1332 2827 3137 3283 4429 1332
Size: 1495 310 146 1146 1522
Fragments arranged by size:
1522 1495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1574 1631 1574
Size: 57 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 704 788 792 872 956 1040 1212 1292
Size: 531 84 4 80 84 84 172 80
Cuts at: 1292 1376 1544 2444 2876 3623 3891 173
Size: 84 168 900 432 747 268 901
Fragments arranged by size:
901 900 747 531 432 268 172 168
84 84 84 84 80 80 4
EcoO109I rG'GnC_Cy
Cuts at: 1758 1758
Size: 4619
EcoRI G'AATT_C
Cuts at: 674 758 926 1094 1178 1262 1346 1514 1623
Size: 84 168 168 84 84 84 168 109
Cuts at: 1623 674
Size: 3670
Fragments arranged by size:
3670 168 168 168 109 84 84 84
84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 2053 2301 3323 4302 2053
Size: 248 1022 979 2370
Fragments arranged by size:
2370 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 282 301 309 314 333 1551 1583 2461
Size: 5 19 8 5 19 1218 32 878
Cuts at: 2461 2491 2493 2523 277
Size: 30 2 30 2373
Fragments arranged by size:
2373 1218 878 32 30 30 19 19
8 5 5 2
HincII GTy'rAC
Cuts at: 1658 1658
Size: 4619
KasI G'GCGC_C
Cuts at: 2049 2297 2049
Size: 248 4371
KpnI G_GTAC'C
Cuts at: 1639 1639
Size: 4619
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 1549 1611 3290 3474 227
Size: 1322 62 1679 184 1372
Fragments arranged by size:
1679 1372 1322 184 62
MscI TGG'CCA
Cuts at: 464 1148 2380 464
Size: 684 1232 2703
Fragments arranged by size:
2703 1232 684
MslI CAynn'nnrTG
Cuts at: 867 1035 1187 1595 2608 2735 867
Size: 168 152 408 1013 127 2751
Fragments arranged by size:
2751 1013 408 168 152 127
MspA1I CmG'CkG
Cuts at: 1730 2044 2404 3417 3662 1730
Size: 314 360 1013 245 2687
Fragments arranged by size:
2687 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 2050 2298 2050
Size: 248 4371
NcoI C'CATG_G
Cuts at: 107 269 107
Size: 162 4457
NdeI CA'TA_TG
Cuts at: 584 864 584
Size: 280 4339
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NspI r_CATG'y
Cuts at: 246 536 1672 2172 3079 246
Size: 290 1136 500 907 1786
Fragments arranged by size:
1786 1136 907 500 290
PciI A'CATG_T
Cuts at: 242 532 3075 242
Size: 290 2543 1786
Fragments arranged by size:
2543 1786 290
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4587
PstI C_TGCA'G
Cuts at: 394 1666 2351 394
Size: 1272 685 2662
Fragments arranged by size:
2662 1272 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 2044 2404 2044
Size: 360 4259
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1576 1633 1576
Size: 57 4562
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 4136 4136
Size: 4619
SfcI C'TryA_G
Cuts at: 33 390 1662 2347 3340 3531 33
Size: 357 1272 685 993 191 1121
Fragments arranged by size:
1272 1121 993 685 357 191
SfoI GGC'GCC
Cuts at: 2051 2299 2051
Size: 248 4371
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1641 1641
Size: 4619
SmlI C'TyrA_G
Cuts at: 348 1774 2689 3181 3443 348
Size: 1426 915 492 262 1524
Fragments arranged by size:
1524 1426 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
StuI AGG'CCT
Cuts at: 721 992 1225 721
Size: 271 233 4115
Fragments arranged by size:
4115 271 233
StyI C'CwwG_G
Cuts at: 107 137 207 269 1753 2107 107
Size: 30 70 62 1484 354 2619
Fragments arranged by size:
2619 1484 354 70 62 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2202 2335 2794 3003 2202
Size: 133 459 209 3818
Fragments arranged by size:
3818 459 209 133
TatI w'GTAC_w
Cuts at: 4134 4134
Size: 4619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 4136 4168 560
Size: 32 3544 32 1011
Fragments arranged by size:
3544 1011 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 405 2114 405
Size: 1709 2910
XmaI C'CCGG_G
Cuts at: 1639 1639
Size: 4619
XmnI GAAnn'nnTTC
Cuts at: 188 648 1057 1225 188
Size: 460 409 168 3582
Fragments arranged by size:
3582 460 409 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI