Restriction map for
pF1KB9612
(Circular) (Six-base) MAPSORT of: pF1KB9612.seq Check: 6652 from: 1 to: 5186
pF1KB9612 5186 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2224 2224
Size: 5186
Acc65I G'GTAC_C
Cuts at: 2202 2202
Size: 5186
AclI AA'CG_TT
Cuts at: 5026 5026
Size: 5186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 823 1462 3011 3443 4190 4458 406
Size: 417 639 1549 432 747 268 1134
Fragments arranged by size:
1549 1134 747 639 432 417 268
AflII C'TTAA_G
Cuts at: 1135 1135
Size: 5186
AflIII A'CryG_T
Cuts at: 2538 3642 4619 2538
Size: 1104 977 3105
Fragments arranged by size:
3105 1104 977
AgeI A'CCGG_T
Cuts at: 3574 3574
Size: 5186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1725 1725
Size: 5186
AlwNI CAG_nnn'CTG
Cuts at: 2491 4058 2491
Size: 1567 3619
ApaLI G'TGCA_C
Cuts at: 3956 3956
Size: 5186
ApoI r'AATT_y
Cuts at: 1070 1359 1453 1720 2190 4539 4795 1070
Size: 289 94 267 470 2349 256 1461
Fragments arranged by size:
2349 1461 470 289 267 256 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 5186
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
AvaI C'yCGr_G
Cuts at: 2206 2206
Size: 5186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1465 1498 1465
Size: 33 5153
BamHI G'GATC_C
Cuts at: 2211 2211
Size: 5186
BanI G'GyrC_C
Cuts at: 2202 2616 2864 2899 2202
Size: 414 248 35 4489
Fragments arranged by size:
4489 414 248 35
BanII G_rGCy'C
Cuts at: 846 1240 2200 3230 846
Size: 394 960 1030 2802
Fragments arranged by size:
2802 1030 960 394
BbeI G_GCGC'C
Cuts at: 2620 2868 2620
Size: 248 4938
BbsI GAAGACnn'nnnn_
Cuts at: 394 1888 1965 394
Size: 1494 77 3615
Fragments arranged by size:
3615 1494 77
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3845 5186 3084
Size: 761 1341 3084
Fragments arranged by size:
3084 1341 761
BclI T'GATC_A
Cuts at: 2706 2706
Size: 5186
BfrBI ATG'CAT
Cuts at: 589 1702 589
Size: 1113 4073
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5186
BglII A'GATC_T
Cuts at: 2701 4738 2701
Size: 2037 3149
BlpI GC'TnA_GC
Cuts at: 2298 2298
Size: 5186
Bme1580I G_kGCm'C
Cuts at: 2811 2904 3960 2811
Size: 93 1056 4037
Fragments arranged by size:
4037 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2398 2479 2814 4958 2398
Size: 81 335 2144 2626
Fragments arranged by size:
2626 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1254 3553 1254
Size: 2299 2887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 481 337
Size: 144 5042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1224 2362 3241 3733 4031 1224
Size: 1138 879 492 298 2379
Fragments arranged by size:
2379 1138 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1824 4890 1824
Size: 3066 2120
BsaAI yAC'GTr
Cuts at: 1513 2539 1513
Size: 1026 4160
BsaBI GATnn'nnATC
Cuts at: 6 2016 2700 4737 6
Size: 2010 684 2037 455
Fragments arranged by size:
2037 2010 684 455
BsaHI Gr'CG_yC
Cuts at: 2617 2865 3567 5063 2617
Size: 248 702 1496 2740
Fragments arranged by size:
2740 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2375 2896 3574 3848 3995 4406 2375
Size: 521 678 274 147 411 3155
Fragments arranged by size:
3155 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 958 2093 958
Size: 1135 4051
BseYI C'CCAG_C
Cuts at: 2611 3369 3946 4442 2611
Size: 758 577 496 3355
Fragments arranged by size:
3355 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2728 2774 3982 104
Size: 2624 46 1208 1308
Fragments arranged by size:
2624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 541 1240 2200 2978 3168 3960 541
Size: 699 960 778 190 792 1767
Fragments arranged by size:
1767 960 792 778 699 190
BsmI GAATG_Cn'
Cuts at: 2074 2074
Size: 5186
Bsp1286I G_dGCh'C
Cuts at: 541 846 1240 2200 2811 2904 2978 3168 3230
Size: 305 394 960 611 93 74 190 62
Cuts at: 3230 3960 541
Size: 730 1767
Fragments arranged by size:
1767 960 730 611 394 305 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4406 4406
Size: 5186
BspHI T'CATG_A
Cuts at: 4362 4362
Size: 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1070 2236 2450 2752 3133 1070
Size: 1166 214 302 381 3123
Fragments arranged by size:
3123 1166 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3478 3532 5016 5181 50
Size: 3428 54 1484 165 55
Fragments arranged by size:
3428 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3098 3098
Size: 5186
BsrFI r'CCGG_y
Cuts at: 3184 3365 3574 3184
Size: 181 209 4796
Fragments arranged by size:
4796 209 181
BsrGI T'GTAC_A
Cuts at: 592 592
Size: 5186
BssSI C'ACGA_G
Cuts at: 3457 3815 3457
Size: 358 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 2563 2563
Size: 5186
BstBI TT'CG_AA
Cuts at: 3547 3547
Size: 5186
BstXI CCAn_nnnn'nTGG
Cuts at: 1340 1340
Size: 5186
BstYI r'GATC_y
Cuts at: 1 2211 2678 2701 3036 3282 4294 4380 4392
Size: 2210 467 23 335 246 1012 86 12
Cuts at: 4392 4738 1
Size: 346 449
Fragments arranged by size:
2210 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4842
Fragments arranged by size:
4842 181 163
BtsI GCAGTG_nn'
Cuts at: 1995 2429 1995
Size: 434 4752
DraI TTT'AAA
Cuts at: 128 351 378 672 1191 2136 2185 128
Size: 223 27 294 519 945 49 3129
Fragments arranged by size:
3129 945 519 294 223 49 27
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3750 2892
Size: 858 4328
EaeI y'GGCC_r
Cuts at: 1127 2771 2945 3336 3363 4708 1127
Size: 1644 174 391 27 1345 1605
Fragments arranged by size:
1644 1605 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5186
EarI CTCTTCn'nnn_
Cuts at: 853 2107 3209 3419 853
Size: 1254 1102 210 2620
Fragments arranged by size:
2620 1254 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3394 3704 3850 4996 3394
Size: 310 146 1146 3584
Fragments arranged by size:
3584 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 2198 1238
Size: 960 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 406 481 823 1462 3011 3443 4190 4458
Size: 69 75 342 639 1549 432 747 268
Cuts at: 4458 337
Size: 1065
Fragments arranged by size:
1549 1065 747 639 432 342 268 75
69
EcoO109I rG'GnC_Cy
Cuts at: 1610 2325 1610
Size: 715 4471
EcoRI G'AATT_C
Cuts at: 1453 2190 1453
Size: 737 4449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1218 1390 1440 1949 3205 1218
Size: 172 50 509 1256 3199
Fragments arranged by size:
3199 1256 509 172 50
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5186
HaeII r_GCGC'y
Cuts at: 2620 2868 3890 4869 2620
Size: 248 1022 979 2937
Fragments arranged by size:
2937 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 952 984 1822 1854 3028 3058 3060 3090 952
Size: 32 838 32 1174 30 2 30 3048
Fragments arranged by size:
3048 1174 838 32 32 30 30 2
HincII GTy'rAC
Cuts at: 707 1767 2225 707
Size: 1060 458 3668
Fragments arranged by size:
3668 1060 458
HindIII A'AGCT_T
Cuts at: 1132 1132
Size: 5186
HpaI GTT'AAC
Cuts at: 1767 1767
Size: 5186
KasI G'GCGC_C
Cuts at: 2616 2864 2616
Size: 248 4938
KpnI G_GTAC'C
Cuts at: 2206 2206
Size: 5186
MfeI C'AATT_G
Cuts at: 1298 1298
Size: 5186
MluI A'CGCG_T
Cuts at: 4619 4619
Size: 5186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3857 4041 3857
Size: 184 5002
MscI TGG'CCA
Cuts at: 1129 2947 1129
Size: 1818 3368
MslI CAynn'nnrTG
Cuts at: 273 2067 3175 3302 273
Size: 1794 1108 127 2157
Fragments arranged by size:
2157 1794 1108 127
MspA1I CmG'CkG
Cuts at: 1046 2297 2611 2971 3984 4229 1046
Size: 1251 314 360 1013 245 2003
Fragments arranged by size:
2003 1251 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3367 3367
Size: 5186
NarI GG'CG_CC
Cuts at: 2617 2865 2617
Size: 248 4938
NdeI CA'TA_TG
Cuts at: 186 186
Size: 5186
NgoMIV G'CCGG_C
Cuts at: 3365 3365
Size: 5186
NsiI A_TGCA'T
Cuts at: 591 1704 591
Size: 1113 4073
NspI r_CATG'y
Cuts at: 593 643 2239 2739 3646 593
Size: 50 1596 500 907 2133
Fragments arranged by size:
2133 1596 907 500 50
PciI A'CATG_T
Cuts at: 3642 3642
Size: 5186
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 5154
PstI C_TGCA'G
Cuts at: 2233 2918 2233
Size: 685 4501
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5186
PvuII CAG'CTG
Cuts at: 1046 2611 2971 1046
Size: 1565 360 3261
Fragments arranged by size:
3261 1565 360
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5186
SacI G_AGCT'C
Cuts at: 1240 2200 1240
Size: 960 4226
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5186
SapI GCTCTTCn'nnn_
Cuts at: 3209 3419 3209
Size: 210 4976
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5186
ScaI AGT'ACT
Cuts at: 4703 4703
Size: 5186
SfcI C'TryA_G
Cuts at: 33 2229 2914 3907 4098 33
Size: 2196 685 993 191 1121
Fragments arranged by size:
2196 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2618 2866 2618
Size: 248 4938
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
SmaI CCC'GGG
Cuts at: 2208 2208
Size: 5186
SmlI C'TyrA_G
Cuts at: 1135 1203 2341 3256 3748 4010 1135
Size: 68 1138 915 492 262 2311
Fragments arranged by size:
2311 1138 915 492 262 68
SnaBI TAC'GTA
Cuts at: 1513 1513
Size: 5186
SpeI A'CTAG_T
Cuts at: 67 306 67
Size: 239 4947
SphI G_CATG'C
Cuts at: 2239 2739 2239
Size: 500 4686
SspI AAT'ATT
Cuts at: 198 582 1626 198
Size: 384 1044 3758
Fragments arranged by size:
3758 1044 384
StuI AGG'CCT
Cuts at: 666 666
Size: 5186
StyI C'CwwG_G
Cuts at: 1498 2320 2674 1498
Size: 822 354 4010
Fragments arranged by size:
4010 822 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2769 2902 3361 3570 2769
Size: 133 459 209 4385
Fragments arranged by size:
4385 459 209 133
TatI w'GTAC_w
Cuts at: 592 642 685 1905 4701 592
Size: 50 43 1220 2796 1077
Fragments arranged by size:
2796 1220 1077 50 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 4703 4735 947
Size: 32 3724 32 1398
Fragments arranged by size:
3724 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 2983 2983
Size: 5186
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1720 2681 1720
Size: 961 4225
XmaI C'CCGG_G
Cuts at: 2206 2206
Size: 5186
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI NcoI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI XmnI ZraI