Restriction map for
pF1KB9608
(Circular) (Six-base) MAPSORT of: pF1KB9608.seq Check: 1227 from: 1 to: 3965
pF1KB9608 3965 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 152 152
Size: 3965
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 705 789 1790 2222 2969 3237 621
Size: 84 84 1001 432 747 268 1349
Fragments arranged by size:
1349 1001 747 432 268 84 84
AflIII A'CryG_T
Cuts at: 1317 2421 3398 1317
Size: 1104 977 1884
Fragments arranged by size:
1884 1104 977
AgeI A'CCGG_T
Cuts at: 863 2353 863
Size: 1490 2475
AleI CACnn'nnGTG
Cuts at: 428 764 428
Size: 336 3629
AlwNI CAG_nnn'CTG
Cuts at: 1270 2837 1270
Size: 1567 2398
ApaLI G'TGCA_C
Cuts at: 783 867 2735 783
Size: 84 1868 2013
Fragments arranged by size:
2013 1868 84
ApoI r'AATT_y
Cuts at: 969 3318 3574 969
Size: 2349 256 1360
Fragments arranged by size:
2349 1360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 302 471 985 302
Size: 169 514 3282
Fragments arranged by size:
3282 514 169
AvrII C'CTAG_G
Cuts at: 594 594
Size: 3965
BamHI G'GATC_C
Cuts at: 203 419 503 990 203
Size: 216 84 487 3178
Fragments arranged by size:
3178 487 216 84
BanI G'GyrC_C
Cuts at: 981 1395 1643 1678 981
Size: 414 248 35 3268
Fragments arranged by size:
3268 414 248 35
BanII G_rGCy'C
Cuts at: 303 901 979 2009 303
Size: 598 78 1030 2259
Fragments arranged by size:
2259 1030 598 78
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BglII A'GATC_T
Cuts at: 1480 3517 1480
Size: 2037 1928
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 369 787 871 1590 1683 2739 369
Size: 418 84 719 93 1056 1595
Fragments arranged by size:
1595 1056 719 418 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 363 1177 1258 1593 3737 363
Size: 814 81 335 2144 591
Fragments arranged by size:
2144 814 591 335 81
BmtI G_CTAG'C
Cuts at: 658 658
Size: 3965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 717 700
Size: 17 3948
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2512 2810 1141
Size: 879 492 298 2296
Fragments arranged by size:
2296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 765 3669 765
Size: 2904 1061
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 1479 3516 6
Size: 1473 2037 455
Fragments arranged by size:
2037 1473 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 668 863 1154 1675 2353 2627 2774 3185 668
Size: 195 291 521 678 274 147 411 1448
Fragments arranged by size:
1448 678 521 411 291 274 195 147
BseYI C'CCAG_C
Cuts at: 943 1390 2148 2725 3221 943
Size: 447 758 577 496 1687
Fragments arranged by size:
1687 758 577 496 447
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2761 104
Size: 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 831 871 901 979 1757 1947 2739 787
Size: 44 40 30 78 778 190 792 2013
Fragments arranged by size:
2013 792 778 190 78 44 40 30
Bsp1286I G_dGCh'C
Cuts at: 303 369 787 831 871 901 979 1590 1683
Size: 66 418 44 40 30 78 611 93
Cuts at: 1683 1757 1947 2009 2739 303
Size: 74 190 62 730 1529
Fragments arranged by size:
1529 730 611 418 190 93 78 74
66 62 44 40 30
BspEI T'CCGG_A
Cuts at: 3185 3185
Size: 3965
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 1015 1229 1531 1912 152
Size: 863 214 302 381 2205
Fragments arranged by size:
2205 863 381 302 214
BsrBI CCG'CTC
Cuts at: 50 501 2257 2311 3795 3960 50
Size: 451 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 451 165 55 54
BsrDI GCAATG_nn'
Cuts at: 320 1877 320
Size: 1557 2408
BsrFI r'CCGG_y
Cuts at: 863 1963 2144 2353 863
Size: 1100 181 209 2475
Fragments arranged by size:
2475 1100 209 181
BssSI C'ACGA_G
Cuts at: 496 2236 2594 496
Size: 1740 358 1867
Fragments arranged by size:
1867 1740 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 1342
Size: 3965
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstYI r'GATC_y
Cuts at: 1 203 419 503 990 1457 1480 1815 2061
Size: 202 216 84 487 467 23 335 246
Cuts at: 2061 3073 3159 3171 3517 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 487 467 449 346 335 246 216
202 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 149 149
Size: 3965
BtgI C'CryG_G
Cuts at: 107 277 107
Size: 170 3795
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 120 1208 120
Size: 1088 2877
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DraIII CAC_nnn'GTG
Cuts at: 791 875 791
Size: 84 3881
DrdI GACnn_nn'nnGTC
Cuts at: 1671 2529 1671
Size: 858 3107
EaeI y'GGCC_r
Cuts at: 604 1550 1724 2115 2142 3487 604
Size: 946 174 391 27 1345 1082
Fragments arranged by size:
1345 1082 946 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 509 709 1988 2198 509
Size: 200 1279 210 2276
Fragments arranged by size:
2276 1279 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 813 901 2173 2483 2629 3775 813
Size: 88 1272 310 146 1146 1003
Fragments arranged by size:
1272 1146 1003 310 146 88
EcoICRI GAG'CTC
Cuts at: 899 977 899
Size: 78 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 700 705 717 789 1790 2222 2969 3237
Size: 79 5 12 72 1001 432 747 268
Cuts at: 3237 621
Size: 1349
Fragments arranged by size:
1349 1001 747 432 268 79 72 12
5
EcoNI CCTnn'n_nnAGG
Cuts at: 148 152 148
Size: 4 3961
EcoO109I rG'GnC_Cy
Cuts at: 315 1104 315
Size: 789 3176
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
EcoRV GAT'ATC
Cuts at: 813 813
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 564 1399 1647 2669 3648 564
Size: 835 248 1022 979 881
Fragments arranged by size:
1022 979 881 835 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 888 920 1807 1837 1839 1869 226
Size: 32 630 32 887 30 2 30 2322
Fragments arranged by size:
2322 887 630 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 422 506 842 2636 2820 224
Size: 198 84 336 1794 184 1369
Fragments arranged by size:
1794 1369 336 198 184 84
MscI TGG'CCA
Cuts at: 1726 1726
Size: 3965
MslI CAynn'nnrTG
Cuts at: 428 764 1954 2081 428
Size: 336 1190 127 2312
Fragments arranged by size:
2312 1190 336 127
MspA1I CmG'CkG
Cuts at: 417 614 837 1076 1390 1750 2763 3008 417
Size: 197 223 239 314 360 1013 245 1374
Fragments arranged by size:
1374 1013 360 314 245 239 223 197
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 107 277 107
Size: 170 3795
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NheI G'CTAG_C
Cuts at: 654 654
Size: 3965
NspI r_CATG'y
Cuts at: 1018 1518 2425 1018
Size: 500 907 2558
Fragments arranged by size:
2558 907 500
PciI A'CATG_T
Cuts at: 2421 2421
Size: 3965
PfoI T'CCnGG_A
Cuts at: 892 892
Size: 3965
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PpuMI rG'GwC_Cy
Cuts at: 315 315
Size: 3965
PstI C_TGCA'G
Cuts at: 1012 1697 1012
Size: 685 3280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 1390 1750 1390
Size: 360 3605
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 901 979 901
Size: 78 3887
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SanDI GG'GwC_CC
Cuts at: 315 315
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 709 1988 2198 709
Size: 1279 210 2476
Fragments arranged by size:
2476 1279 210
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SfcI C'TryA_G
Cuts at: 33 1008 1693 2686 2877 33
Size: 975 685 993 191 1121
Fragments arranged by size:
1121 993 975 685 191
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 302 471 1120 2035 2527 2789 302
Size: 169 649 915 492 262 1478
Fragments arranged by size:
1478 915 649 492 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 107 172 277 331 594 1099 1453 107
Size: 65 105 54 263 505 354 2619
Fragments arranged by size:
2619 505 354 263 105 65 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 772 1548 1681 2140 2349 772
Size: 776 133 459 209 2388
Fragments arranged by size:
2388 776 459 209 133
TatI w'GTAC_w
Cuts at: 3480 3480
Size: 3965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 275 1460 275
Size: 1185 2780
XhoI C'TCGA_G
Cuts at: 302 471 302
Size: 169 3796
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
XmnI GAAnn'nnTTC
Cuts at: 718 886 718
Size: 168 3797
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI