Restriction map for
pF1KB9599
(Circular) (Six-base) MAPSORT of: pF1KB9599.seq Check: 7589 from: 1 to: 4430
pF1KB9599 4430 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 264 746 264
Size: 482 3948
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 2255 2687 3434 3702 416
Size: 1839 432 747 268 1144
Fragments arranged by size:
1839 1144 747 432 268
AflIII A'CryG_T
Cuts at: 665 1782 2886 3863 665
Size: 1117 1104 977 1232
Fragments arranged by size:
1232 1117 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 1116 1735 3302 1116
Size: 619 1567 2244
Fragments arranged by size:
2244 1567 619
ApaI G_GGCC'C
Cuts at: 813 1421 813
Size: 608 3822
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 1434 3783 4039 1434
Size: 2349 256 1825
Fragments arranged by size:
2349 1825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 305 333 726 834 859 1450 305
Size: 28 393 108 25 591 3285
Fragments arranged by size:
3285 591 393 108 28 25
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 355 369 936 975 1268 1446 1860 2108 2143
Size: 14 567 39 293 178 414 248 35
Cuts at: 2143 355
Size: 2642
Fragments arranged by size:
2642 567 414 293 248 178 39 35
14
BanII G_rGCy'C
Cuts at: 735 813 1321 1421 1444 2474 735
Size: 78 508 100 23 1030 2691
Fragments arranged by size:
2691 1030 508 100 78 23
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4182
BbsI GAAGACnn'nnnn_
Cuts at: 182 221 182
Size: 39 4391
BbvCI CC'TCA_GC
Cuts at: 1009 1009
Size: 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 880 914 880
Size: 34 4396
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BfrBI ATG'CAT
Cuts at: 1060 1060
Size: 4430
BglI GCCn_nnn'nGGC
Cuts at: 623 1285 623
Size: 662 3768
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 125 487 1542 125
Size: 362 1055 3013
Fragments arranged by size:
3013 1055 362
Bme1580I G_kGCm'C
Cuts at: 372 813 939 1271 1421 2055 2148 3204 372
Size: 441 126 332 150 634 93 1056 1598
Fragments arranged by size:
1598 1056 634 441 332 150 126 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1380 2797 1380
Size: 1417 3013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 660 1146 244
Size: 416 486 3528
Fragments arranged by size:
3528 486 416
Bpu10I CC'TnA_GC
Cuts at: 1009 1009
Size: 4430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2485 2977 3275 1606
Size: 879 492 298 2761
Fragments arranged by size:
2761 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4134 4134
Size: 4430
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 867 1038 1861 2109 2811 4307 867
Size: 171 823 248 702 1496 990
Fragments arranged by size:
1496 990 823 702 248 171
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 4400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 235 238 417 887 1035 1231 217
Size: 18 3 179 470 148 196 3416
Fragments arranged by size:
3416 470 196 179 148 18 3
BseYI C'CCAG_C
Cuts at: 1260 1855 2613 3190 3686 1260
Size: 595 758 577 496 2004
Fragments arranged by size:
2004 758 595 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 763 868 749
Size: 14 105 4311
Fragments arranged by size:
4311 105 14
BsiEI CG_ry'CG
Cuts at: 104 1138 1170 1280 1972 2018 3226 104
Size: 1034 32 110 692 46 1208 1308
Fragments arranged by size:
1308 1208 1034 692 110 46 32
BsiHKAI G_wGCw'C
Cuts at: 314 1444 2222 2412 3204 314
Size: 1130 778 190 792 1540
Fragments arranged by size:
1540 1130 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 858 858
Size: 4430
Bsp1286I G_dGCh'C
Cuts at: 314 372 735 813 939 1271 1321 1421 1444
Size: 58 363 78 126 332 50 100 23
Cuts at: 1444 2055 2148 2222 2412 2474 3204 314
Size: 611 93 74 190 62 730 1540
Fragments arranged by size:
1540 730 611 363 332 190 126 100
93 78 74 62 58 50 23
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 746 768 1480 1694 1996 2377 264
Size: 482 22 712 214 302 381 2317
Fragments arranged by size:
2317 712 482 381 302 214 22
BsrBI CCG'CTC
Cuts at: 50 1104 2722 2776 4260 4425 50
Size: 1054 1618 54 1484 165 55
Fragments arranged by size:
1618 1484 1054 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 411 2428 2609 2818 411
Size: 2017 181 209 2023
Fragments arranged by size:
2023 2017 209 181
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstYI r'GATC_y
Cuts at: 1 599 1455 1922 1945 2280 2526 3538 3624
Size: 598 856 467 23 335 246 1012 86
Cuts at: 3624 3636 3982 1
Size: 12 346 449
Fragments arranged by size:
1012 856 598 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 449 449
Size: 4430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 420 1316 2380 2561 2724 420
Size: 896 1064 181 163 2126
Fragments arranged by size:
2126 1064 896 181 163
BtsI GCAGTG_nn'
Cuts at: 1673 1673
Size: 4430
ClaI AT'CG_AT
Cuts at: 188 188
Size: 4430
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 592 2136 2994 592
Size: 1544 858 2028
Fragments arranged by size:
2028 1544 858
EaeI y'GGCC_r
Cuts at: 452 513 1277 1337 1403 2015 2189 2580 2607
Size: 61 764 60 66 612 174 391 27
Cuts at: 2607 3952 452
Size: 1345 930
Fragments arranged by size:
1345 930 764 612 391 174 66 61
60 27
EagI C'GGCC_G
Cuts at: 1277 2015 1277
Size: 738 3692
EarI CTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 553 1069 1367 2638 2948 3094 4240 553
Size: 516 298 1271 310 146 1146 743
Fragments arranged by size:
1271 1146 743 516 310 298 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 416 660 1146 2255 2687 3434 3702 244
Size: 172 244 486 1109 432 747 268 972
Fragments arranged by size:
1109 972 747 486 432 268 244 172
EcoNI CCTnn'n_nnAGG
Cuts at: 199 199
Size: 4430
EcoO109I rG'GnC_Cy
Cuts at: 809 810 839 1361 1417 1418 1569 809
Size: 1 29 522 56 1 151 3670
Fragments arranged by size:
3670 522 151 56 29 1 1
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4430
FspI TGC'GCA
Cuts at: 1120 2211 1120
Size: 1091 3339
HaeII r_GCGC'y
Cuts at: 132 480 714 1291 1864 2112 3134 4113 132
Size: 348 234 577 573 248 1022 979 449
Fragments arranged by size:
1022 979 577 573 449 348 248 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 299 331 640 672 2272 2302 2304 2334 299
Size: 32 309 32 1600 30 2 30 2395
Fragments arranged by size:
2395 1600 309 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4182
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1162 3101 3285 1162
Size: 1939 184 2307
Fragments arranged by size:
2307 1939 184
MscI TGG'CCA
Cuts at: 454 2191 454
Size: 1737 2693
MslI CAynn'nnrTG
Cuts at: 448 2419 2546 448
Size: 1971 127 2332
Fragments arranged by size:
2332 1971 127
MspA1I CmG'CkG
Cuts at: 491 1206 1541 1855 2215 3228 3473 491
Size: 715 335 314 360 1013 245 1448
Fragments arranged by size:
1448 1013 715 360 335 314 245
NaeI GCC'GGC
Cuts at: 413 2611 413
Size: 2198 2232
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4182
NcoI C'CATG_G
Cuts at: 449 449
Size: 4430
NgoMIV G'CCGG_C
Cuts at: 411 2609 411
Size: 2198 2232
NsiI A_TGCA'T
Cuts at: 1062 1062
Size: 4430
NspI r_CATG'y
Cuts at: 669 1483 1983 2890 669
Size: 814 500 907 2209
Fragments arranged by size:
2209 907 814 500
PciI A'CATG_T
Cuts at: 665 2886 665
Size: 2221 2209
PfoI T'CCnGG_A
Cuts at: 158 158
Size: 4430
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PpuMI rG'GwC_Cy
Cuts at: 1361 1361
Size: 4430
PspOMI G'GGCC_C
Cuts at: 809 1417 809
Size: 608 3822
PstI C_TGCA'G
Cuts at: 275 994 1477 2162 275
Size: 719 483 685 2543
Fragments arranged by size:
2543 719 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 1855 2215 1855
Size: 360 4070
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 271 990 1473 2158 3151 3342 33
Size: 238 719 483 685 993 191 1121
Fragments arranged by size:
1121 993 719 685 483 238 191
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 728 836 861 1452 728
Size: 108 25 591 3706
Fragments arranged by size:
3706 591 108 25
SmlI C'TyrA_G
Cuts at: 1585 2500 2992 3254 1585
Size: 915 492 262 2761
Fragments arranged by size:
2761 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
StyI C'CwwG_G
Cuts at: 376 449 1014 1247 1564 1918 376
Size: 73 565 233 317 354 2888
Fragments arranged by size:
2888 565 354 317 233 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2013 2146 2605 2814 2013
Size: 133 459 209 3629
Fragments arranged by size:
3629 459 209 133
TatI w'GTAC_w
Cuts at: 3945 3945
Size: 4430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 4398
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4430
XmaI C'CCGG_G
Cuts at: 726 834 859 1450 726
Size: 108 25 591 3706
Fragments arranged by size:
3706 591 108 25
XmnI GAAnn'nnTTC
Cuts at: 703 703
Size: 4430
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI