Restriction map for
pF1KB9598
(Circular) (Six-base) MAPSORT of: pF1KB9598.seq Check: 141 from: 1 to: 4547
pF1KB9598 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 848 848
Size: 4547
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2804 3551 3819 2372
Size: 432 747 268 3100
Fragments arranged by size:
3100 747 432 268
AfeI AGC'GCT
Cuts at: 1388 1388
Size: 4547
AflIII A'CryG_T
Cuts at: 140 776 1899 3003 3980 140
Size: 636 1123 1104 977 707
Fragments arranged by size:
1123 1104 977 707 636
AgeI A'CCGG_T
Cuts at: 1041 2935 1041
Size: 1894 2653
AhdI GACnn_n'nnGTC
Cuts at: 765 765
Size: 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 342 1476 1852 3419 342
Size: 1134 376 1567 1470
Fragments arranged by size:
1567 1470 1134 376
ApaI G_GGCC'C
Cuts at: 1270 1270
Size: 4547
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AscI GG'CGCG_CC
Cuts at: 398 398
Size: 4547
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 501 732 1074 1122 1366 1567 501
Size: 231 342 48 244 201 3481
Fragments arranged by size:
3481 342 244 231 201 48
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 288 300 803 904 958 1038 1153 1183 1192
Size: 12 503 101 54 80 115 30 9
Cuts at: 1192 1563 1977 2225 2260 288
Size: 371 414 248 35 2575
Fragments arranged by size:
2575 503 414 371 248 115 101 80
54 35 30 12 9
BanII G_rGCy'C
Cuts at: 580 1270 1288 1561 2591 580
Size: 690 18 273 1030 2536
Fragments arranged by size:
2536 1030 690 273 18
BbeI G_GCGC'C
Cuts at: 807 908 1157 1187 1981 2229 807
Size: 101 249 30 794 248 3125
Fragments arranged by size:
3125 794 249 248 101 30
BbvCI CC'TCA_GC
Cuts at: 320 320
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 932 1162 932
Size: 230 4317
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1417 1659 1417
Size: 242 4305
Bme1580I G_kGCm'C
Cuts at: 293 1177 1195 1270 2172 2265 3321 293
Size: 884 18 75 902 93 1056 1519
Fragments arranged by size:
1519 1056 902 884 93 75 18
BmgBI CAC'GTC
Cuts at: 174 393 174
Size: 219 4328
BmrI ACTGGGnnnn_n'
Cuts at: 1328 1759 1840 2175 4319 1328
Size: 431 81 335 2144 1556
Fragments arranged by size:
2144 1556 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 994
Size: 4547
Bpu10I CC'TnA_GC
Cuts at: 320 598 320
Size: 278 4269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 1723 2602 3094 3392 1505
Size: 218 879 492 298 2660
Fragments arranged by size:
2660 879 492 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 4251 4251
Size: 4547
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 804 905 1154 1184 1978 2226 2928 4424 804
Size: 101 249 30 794 248 702 1496 927
Fragments arranged by size:
1496 927 794 702 249 248 101 30
BsaWI w'CCGG_w
Cuts at: 1041 1465 1736 2257 2935 3209 3356 3767 1041
Size: 424 271 521 678 274 147 411 1821
Fragments arranged by size:
1821 678 521 424 411 274 271 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 454
Size: 4547
BseYI C'CCAG_C
Cuts at: 1083 1300 1972 2730 3307 3803 1083
Size: 217 672 758 577 496 1827
Fragments arranged by size:
1827 758 672 577 496 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1396 922
Size: 474 4073
BsiEI CG_ry'CG
Cuts at: 104 167 365 902 924 956 989 1100 1166
Size: 63 198 537 22 32 33 111 66
Cuts at: 1166 1234 1496 1514 2089 2135 3343 104
Size: 68 262 18 575 46 1208 1308
Fragments arranged by size:
1308 1208 575 537 262 198 111 68
66 63 46 33 32 22 18
BsiHKAI G_wGCw'C
Cuts at: 839 1561 2339 2529 3321 839
Size: 722 778 190 792 2065
Fragments arranged by size:
2065 792 778 722 190
BsiWI C'GTAC_G
Cuts at: 1019 1019
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 293 580 839 1177 1195 1270 1288 1561 2172
Size: 287 259 338 18 75 18 273 611
Cuts at: 2172 2265 2339 2529 2591 3321 293
Size: 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 338 287 273 259 190
93 75 74 62 18 18
BspEI T'CCGG_A
Cuts at: 1465 3767 1465
Size: 2302 2245
BspHI T'CATG_A
Cuts at: 860 3723 860
Size: 2863 1684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 223 848 1597 1811 2113 2494 223
Size: 625 749 214 302 381 2276
Fragments arranged by size:
2276 749 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 455 602 1519 2839 2893 4377 4542 50
Size: 405 147 917 1320 54 1484 165 55
Fragments arranged by size:
1484 1320 917 405 165 147 55 54
BsrDI GCAATG_nn'
Cuts at: 346 2459 346
Size: 2113 2434
BsrFI r'CCGG_y
Cuts at: 901 955 1032 1041 1309 1533 2545 2726 2935
Size: 54 77 9 268 224 1012 181 209
Cuts at: 2935 901
Size: 2513
Fragments arranged by size:
2513 1012 268 224 209 181 77 54
9
BssHII G'CGCG_C
Cuts at: 398 1226 398
Size: 828 3719
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstYI r'GATC_y
Cuts at: 1 710 1572 2039 2062 2397 2643 3655 3741
Size: 709 862 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1012 862 709 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 248 560 908 989 1160 1187 1271 248
Size: 312 348 81 171 27 84 3524
Fragments arranged by size:
3524 348 312 171 84 81 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 531 1422 2497 2678 2841 531
Size: 891 1075 181 163 2237
Fragments arranged by size:
2237 1075 891 181 163
BtsI GCAGTG_nn'
Cuts at: 1790 1790
Size: 4547
ClaI AT'CG_AT
Cuts at: 182 182
Size: 4547
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 703 1377 2253 3111 703
Size: 674 876 858 2139
Fragments arranged by size:
2139 876 858 674
EaeI y'GGCC_r
Cuts at: 147 164 362 563 899 921 953 986 1097
Size: 17 198 201 336 22 32 33 111
Cuts at: 1097 1163 1231 1360 1394 1493 1511 1531 2132
Size: 66 68 129 34 99 18 20 601
Cuts at: 2132 2306 2697 2724 4069 147
Size: 174 391 27 1345 625
Fragments arranged by size:
1345 625 601 391 336 201 198 174
129 111 99 68 66 34 33 32
27 22 20 18 17
EagI C'GGCC_G
Cuts at: 164 362 899 921 953 986 1097 1163 1231
Size: 198 537 22 32 33 111 66 68
Cuts at: 1231 1493 1511 2132 164
Size: 262 18 621 2579
Fragments arranged by size:
2579 621 537 262 198 111 68 66
33 32 22 18
EarI CTCTTCn'nnn_
Cuts at: 209 2570 2780 209
Size: 2361 210 1976
Fragments arranged by size:
2361 1976 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 424 664 2755 3065 3211 4357 295
Size: 129 240 2091 310 146 1146 485
Fragments arranged by size:
2091 1146 485 310 240 146 129
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 2372 2804 3551 3819 994
Size: 1378 432 747 268 1722
Fragments arranged by size:
1722 1378 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 274 639 274
Size: 365 4182
EcoO109I rG'GnC_Cy
Cuts at: 913 1266 1267 1686 913
Size: 353 1 419 3774
Fragments arranged by size:
3774 419 353 1
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 134 807 825 881 908 1009 1157 1187 1301
Size: 673 18 56 27 101 148 30 114
Cuts at: 1301 1390 1981 2229 3251 4230 134
Size: 89 591 248 1022 979 451
Fragments arranged by size:
1022 979 673 591 451 248 148 114
101 89 56 30 27 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 2389 2419 2421 2451 751
Size: 32 1606 30 2 30 2847
Fragments arranged by size:
2847 1606 32 30 30 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
KasI G'GCGC_C
Cuts at: 803 904 1153 1183 1977 2225 803
Size: 101 249 30 794 248 3125
Fragments arranged by size:
3125 794 249 248 101 30
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3218 3402 3218
Size: 184 4363
MscI TGG'CCA
Cuts at: 565 1396 2308 565
Size: 831 912 2804
Fragments arranged by size:
2804 912 831
MslI CAynn'nnrTG
Cuts at: 184 559 2536 2663 184
Size: 375 1977 127 2068
Fragments arranged by size:
2068 1977 375 127
MspA1I CmG'CkG
Cuts at: 250 479 910 979 991 1057 1065 1134 1162
Size: 229 431 69 12 66 8 69 28
Cuts at: 1162 1168 1180 1189 1273 1410 1504 1658 1972
Size: 6 12 9 84 137 94 154 314
Cuts at: 1972 2332 3345 3590 250
Size: 360 1013 245 1207
Fragments arranged by size:
1207 1013 431 360 314 245 229 154
137 94 84 69 69 66 28 12
12 9 8 6
NaeI GCC'GGC
Cuts at: 903 1311 1535 2728 903
Size: 408 224 1193 2722
Fragments arranged by size:
2722 1193 408 224
NarI GG'CG_CC
Cuts at: 804 905 1154 1184 1978 2226 804
Size: 101 249 30 794 248 3125
Fragments arranged by size:
3125 794 249 248 101 30
NcoI C'CATG_G
Cuts at: 560 560
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 901 1309 1533 2726 901
Size: 408 224 1193 2722
Fragments arranged by size:
2722 1193 408 224
NotI GC'GGCC_GC
Cuts at: 986 1097 1163 1511 986
Size: 111 66 348 4022
Fragments arranged by size:
4022 348 111 66
NspI r_CATG'y
Cuts at: 144 780 1600 2100 3007 144
Size: 636 820 500 907 1684
Fragments arranged by size:
1684 907 820 636 500
PciI A'CATG_T
Cuts at: 140 776 3003 140
Size: 636 2227 1684
Fragments arranged by size:
2227 1684 636
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 795 3686 3718 763
Size: 32 2891 32 1592
Fragments arranged by size:
2891 1592 32 32
PpuMI rG'GwC_Cy
Cuts at: 913 913
Size: 4547
PspOMI G'GGCC_C
Cuts at: 1266 1266
Size: 4547
PstI C_TGCA'G
Cuts at: 575 1133 1139 1481 1594 2279 575
Size: 558 6 342 113 685 2843
Fragments arranged by size:
2843 685 558 342 113 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1134 1972 2332 1134
Size: 838 360 3349
Fragments arranged by size:
3349 838 360
RsrII CG'GwC_CG
Cuts at: 379 2742 379
Size: 2363 2184
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SacII CC_GC'GG
Cuts at: 251 911 992 1163 1190 1274 251
Size: 660 81 171 27 84 3524
Fragments arranged by size:
3524 660 171 84 81 27
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SanDI GG'GwC_CC
Cuts at: 913 913
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 571 639 1129 1135 1477 1590 2275 3268
Size: 538 68 490 6 342 113 685 993
Cuts at: 3268 3459 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 538 490 342 191 113
68 6
SfoI GGC'GCC
Cuts at: 805 906 1155 1185 1979 2227 805
Size: 101 249 30 794 248 3125
Fragments arranged by size:
3125 794 249 248 101 30
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SgrAI Cr'CCGG_yG
Cuts at: 1032 1041 1309 1032
Size: 9 268 4270
Fragments arranged by size:
4270 268 9
SmaI CCC'GGG
Cuts at: 734 1124 1368 1569 734
Size: 390 244 201 3712
Fragments arranged by size:
3712 390 244 201
SmlI C'TyrA_G
Cuts at: 1520 1702 2617 3109 3371 1520
Size: 182 915 492 262 2696
Fragments arranged by size:
2696 915 492 262 182
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
SrfI GCCC'GGGC
Cuts at: 1124 1124
Size: 4547
StuI AGG'CCT
Cuts at: 494 494
Size: 4547
StyI C'CwwG_G
Cuts at: 353 560 1681 2035 353
Size: 207 1121 354 2865
Fragments arranged by size:
2865 1121 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1414 2130 2263 2722 2931 1414
Size: 716 133 459 209 3030
Fragments arranged by size:
3030 716 459 209 133
TatI w'GTAC_w
Cuts at: 4062 4062
Size: 4547
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2042 2042
Size: 4547
XmaI C'CCGG_G
Cuts at: 732 1122 1366 1567 732
Size: 390 244 201 3712
Fragments arranged by size:
3712 390 244 201
XmnI GAAnn'nnTTC
Cuts at: 814 814
Size: 4547
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AvrII BaeI BbsI BcgI
BfrBI BmtI BsaXI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SnaBI SspI SwaI XhoI ZraI