Restriction map for
pF1KB9594
(Circular) (Six-base) MAPSORT of: pF1KB9594.seq Check: 8177 from: 1 to: 4088
pF1KB9594 4088 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 554 554
Size: 4088
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 1104 1104
Size: 4088
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
AfeI AGC'GCT
Cuts at: 274 430 274
Size: 156 3932
AflII C'TTAA_G
Cuts at: 442 442
Size: 4088
AflIII A'CryG_T
Cuts at: 128 392 756 758 1440 2544 3521 128
Size: 264 364 2 682 1104 977 695
Fragments arranged by size:
1104 977 695 682 364 264 2
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 562 430
Size: 132 3956
AlwNI CAG_nnn'CTG
Cuts at: 365 492 1393 2960 365
Size: 127 901 1567 1493
Fragments arranged by size:
1567 1493 901 127
ApaLI G'TGCA_C
Cuts at: 1078 2858 1078
Size: 1780 2308
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 196 534 597 1108 196
Size: 338 63 511 3176
Fragments arranged by size:
3176 511 338 63
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 419 459 687 742 1104 1518 1766 1801 419
Size: 40 228 55 362 414 248 35 2706
Fragments arranged by size:
2706 414 362 248 228 55 40 35
BanII G_rGCy'C
Cuts at: 193 1102 2132 193
Size: 909 1030 2149
Fragments arranged by size:
2149 1030 909
BbeI G_GCGC'C
Cuts at: 423 463 1522 1770 423
Size: 40 1059 248 2741
Fragments arranged by size:
2741 1059 248 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4054
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglI GCCn_nnn'nGGC
Cuts at: 481 660 481
Size: 179 3909
BglII A'GATC_T
Cuts at: 221 1603 3640 221
Size: 1382 2037 669
Fragments arranged by size:
2037 1382 669
BlpI GC'TnA_GC
Cuts at: 214 517 979 1200 214
Size: 303 462 221 3102
Fragments arranged by size:
3102 462 303 221
Bme1580I G_kGCm'C
Cuts at: 541 692 745 893 920 1082 1713 1806 2862
Size: 151 53 148 27 162 631 93 1056
Cuts at: 2862 541
Size: 1767
Fragments arranged by size:
1767 1056 631 162 151 148 93 53
27
BmgBI CAC'GTC
Cuts at: 989 989
Size: 4088
BmrI ACTGGGnnnn_n'
Cuts at: 1300 1381 1716 3860 1300
Size: 81 335 2144 1528
Fragments arranged by size:
2144 1528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2635 2933 1264
Size: 879 492 298 2419
Fragments arranged by size:
2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3792 3792
Size: 4088
BsaAI yAC'GTr
Cuts at: 131 759 1441 131
Size: 628 682 2778
Fragments arranged by size:
2778 682 628
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 420 460 1519 1767 2469 3965 420
Size: 40 1059 248 702 1496 543
Fragments arranged by size:
1496 1059 702 543 248 40
BsaWI w'CCGG_w
Cuts at: 1277 1798 2476 2750 2897 3308 1277
Size: 521 678 274 147 411 2057
Fragments arranged by size:
2057 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 286
Size: 30 4058
BseYI C'CCAG_C
Cuts at: 463 738 1030 1513 2271 2848 3344 463
Size: 275 292 483 758 577 496 1207
Fragments arranged by size:
1207 758 577 496 483 292 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 358
Size: 4088
BsiEI CG_ry'CG
Cuts at: 104 123 340 911 1630 1676 2884 104
Size: 19 217 571 719 46 1208 1308
Fragments arranged by size:
1308 1208 719 571 217 46 19
BsiHKAI G_wGCw'C
Cuts at: 193 1082 1102 1880 2070 2862 193
Size: 889 20 778 190 792 1419
Fragments arranged by size:
1419 889 792 778 190 20
Bsp1286I G_dGCh'C
Cuts at: 193 541 692 745 893 920 1082 1102 1713
Size: 348 151 53 148 27 162 20 611
Cuts at: 1713 1806 1880 2070 2132 2862 193
Size: 93 74 190 62 730 1419
Fragments arranged by size:
1419 730 611 348 190 162 151 148
93 74 62 53 27 20
BspEI T'CCGG_A
Cuts at: 3308 3308
Size: 4088
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 554 1138 1352 1654 2035 500
Size: 54 584 214 302 381 2553
Fragments arranged by size:
2553 584 381 302 214 54
BsrBI CCG'CTC
Cuts at: 50 705 2380 2434 3918 4083 50
Size: 655 1675 54 1484 165 55
Fragments arranged by size:
1675 1484 655 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 684 745 772 906 2086 2267 2476 684
Size: 61 27 134 1180 181 209 2296
Fragments arranged by size:
2296 1180 209 181 134 61 27
BssHII G'CGCG_C
Cuts at: 521 521
Size: 4088
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 492 687 1465 492
Size: 195 778 3115
Fragments arranged by size:
3115 778 195
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 804 804
Size: 4088
BstYI r'GATC_y
Cuts at: 1 221 326 1113 1580 1603 1938 2184 3196
Size: 220 105 787 467 23 335 246 1012
Cuts at: 3196 3282 3294 3640 1
Size: 86 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 246 220
105 86 23 12
BtgI C'CryG_G
Cuts at: 331 725 803 331
Size: 394 78 3616
Fragments arranged by size:
3616 394 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 618 2038 2219 2382 618
Size: 1420 181 163 2324
Fragments arranged by size:
2324 1420 181 163
BtsI GCAGTG_nn'
Cuts at: 1331 1331
Size: 4088
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DraIII CAC_nnn'GTG
Cuts at: 1071 1071
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 120 179 337 751 788 908 1673 1847 2238
Size: 59 158 414 37 120 765 174 391
Cuts at: 2238 2265 3610 120
Size: 27 1345 598
Fragments arranged by size:
1345 765 598 414 391 174 158 120
59 37 27
EagI C'GGCC_G
Cuts at: 120 337 908 1673 120
Size: 217 571 765 2535
Fragments arranged by size:
2535 765 571 217
EarI CTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 663 759 1047 2296 2606 2752 3898 663
Size: 96 288 1249 310 146 1146 853
Fragments arranged by size:
1249 1146 853 310 288 146 96
EcoICRI GAG'CTC
Cuts at: 191 1100 191
Size: 909 3179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 255 255
Size: 4088
EcoO109I rG'GnC_Cy
Cuts at: 1227 1227
Size: 4088
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 378 630 2107 378
Size: 252 1477 2359
Fragments arranged by size:
2359 1477 252
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 276 373 423 432 463 827 943 1522 1770
Size: 97 50 9 31 364 116 579 248
Cuts at: 1770 2792 3771 276
Size: 1022 979 593
Fragments arranged by size:
1022 979 593 579 364 248 116 97
50 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1930 1960 1962 1992 1930
Size: 30 2 30 4026
Fragments arranged by size:
4026 30 30 2
HincII GTy'rAC
Cuts at: 719 1127 719
Size: 408 3680
KasI G'GCGC_C
Cuts at: 419 459 1518 1766 419
Size: 40 1059 248 2741
Fragments arranged by size:
2741 1059 248 40
KpnI G_GTAC'C
Cuts at: 1108 1108
Size: 4088
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 2759 2943 473
Size: 2286 184 1618
Fragments arranged by size:
2286 1618 184
MscI TGG'CCA
Cuts at: 181 753 1849 181
Size: 572 1096 2420
Fragments arranged by size:
2420 1096 572
MslI CAynn'nnrTG
Cuts at: 761 802 886 2077 2204 761
Size: 41 84 1191 127 2645
Fragments arranged by size:
2645 1191 127 84 41
MspA1I CmG'CkG
Cuts at: 213 333 694 849 1199 1513 1873 2886 3131
Size: 120 361 155 350 314 360 1013 245
Cuts at: 3131 213
Size: 1170
Fragments arranged by size:
1170 1013 361 360 350 314 245 155
120
NaeI GCC'GGC
Cuts at: 774 908 2269 774
Size: 134 1361 2593
Fragments arranged by size:
2593 1361 134
NarI GG'CG_CC
Cuts at: 420 460 1519 1767 420
Size: 40 1059 248 2741
Fragments arranged by size:
2741 1059 248 40
NcoI C'CATG_G
Cuts at: 725 803 725
Size: 78 4010
NgoMIV G'CCGG_C
Cuts at: 772 906 2267 772
Size: 134 1361 2593
Fragments arranged by size:
2593 1361 134
NspI r_CATG'y
Cuts at: 396 1141 1641 2548 396
Size: 745 500 907 1936
Fragments arranged by size:
1936 907 745 500
PciI A'CATG_T
Cuts at: 392 2544 392
Size: 2152 1936
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PmlI CAC'GTG
Cuts at: 759 759
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PstI C_TGCA'G
Cuts at: 497 1135 1820 497
Size: 638 685 2765
Fragments arranged by size:
2765 685 638
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 1513 1873 1513
Size: 360 3728
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 193 1102 193
Size: 909 3179
SacII CC_GC'GG
Cuts at: 334 334
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 255 493 819 1131 1816 2809 3000 33
Size: 222 238 326 312 685 993 191 1121
Fragments arranged by size:
1121 993 685 326 312 238 222 191
SfoI GGC'GCC
Cuts at: 421 461 1520 1768 421
Size: 40 1059 248 2741
Fragments arranged by size:
2741 1059 248 40
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 442 1243 2158 2650 2912 442
Size: 801 915 492 262 1618
Fragments arranged by size:
1618 915 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StyI C'CwwG_G
Cuts at: 725 803 1222 1576 725
Size: 78 419 354 3237
Fragments arranged by size:
3237 419 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 392 1671 1804 2263 2472 392
Size: 1279 133 459 209 2008
Fragments arranged by size:
2008 1279 459 209 133
TatI w'GTAC_w
Cuts at: 3603 3603
Size: 4088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 286 318 3605 3637 286
Size: 32 3287 32 737
Fragments arranged by size:
3287 737 32 32
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AhdI AleI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI BpmI Bpu10I BseRI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI