Restriction map for
pF1KB9593
(Circular) (Six-base) MAPSORT of: pF1KB9593.seq Check: 5880 from: 1 to: 4103
pF1KB9593 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1000 1000
Size: 4103
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
AflIII A'CryG_T
Cuts at: 407 1455 2559 3536 407
Size: 1048 1104 977 974
Fragments arranged by size:
1104 1048 977 974
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 479 479
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 123 380 466 787 870 1408 2975 123
Size: 257 86 321 83 538 1567 1251
Fragments arranged by size:
1567 1251 538 321 257 86 83
ApaI G_GGCC'C
Cuts at: 484 969 484
Size: 485 3618
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 873 1107 3456 3712 873
Size: 234 2349 256 1264
Fragments arranged by size:
2349 1264 256 234
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 127 476 1123 127
Size: 349 647 3107
Fragments arranged by size:
3107 647 349
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 136 342 472 1119 1533 1781 1816 136
Size: 206 130 647 414 248 35 2423
Fragments arranged by size:
2423 647 414 248 206 130 35
BanII G_rGCy'C
Cuts at: 208 484 683 725 969 1117 2147 208
Size: 276 199 42 244 148 1030 2164
Fragments arranged by size:
2164 1030 276 244 199 148 42
BbeI G_GCGC'C
Cuts at: 140 1537 1785 140
Size: 1397 248 2458
Fragments arranged by size:
2458 1397 248
BbvCI CC'TCA_GC
Cuts at: 467 918 467
Size: 451 3652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4069
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 291 291
Size: 4103
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 347 475 484 643 969 1728 1821 2877 347
Size: 128 9 159 326 759 93 1056 1573
Fragments arranged by size:
1573 1056 759 326 159 128 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 935
Size: 4103
Bpu10I CC'TnA_GC
Cuts at: 467 598 918 467
Size: 131 320 3652
Fragments arranged by size:
3652 320 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1279 2158 2650 2948 922
Size: 357 879 492 298 2077
Fragments arranged by size:
2077 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 137 1075 1534 1782 2484 3980 137
Size: 938 459 248 702 1496 260
Fragments arranged by size:
1496 938 702 459 260 248
BsaWI w'CCGG_w
Cuts at: 1070 1292 1813 2491 2765 2912 3323 1070
Size: 222 521 678 274 147 411 1850
Fragments arranged by size:
1850 678 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 147 177 147
Size: 30 4073
BseYI C'CCAG_C
Cuts at: 642 753 1528 2286 2863 3359 642
Size: 111 775 758 577 496 1386
Fragments arranged by size:
1386 775 758 577 496 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 920
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 551 1645 1691 2899 104
Size: 447 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 447 46
BsiHKAI G_wGCw'C
Cuts at: 208 419 565 725 908 1117 1895 2085 2877
Size: 211 146 160 183 209 778 190 792
Cuts at: 2877 208
Size: 1434
Fragments arranged by size:
1434 792 778 211 209 190 183 160
146
Bsp1286I G_dGCh'C
Cuts at: 208 347 419 475 484 565 643 683 725
Size: 139 72 56 9 81 78 40 42
Cuts at: 725 908 969 1117 1728 1821 1895 2085 2147
Size: 183 61 148 611 93 74 190 62
Cuts at: 2147 2877 208
Size: 730 1434
Fragments arranged by size:
1434 730 611 190 183 148 139 93
81 78 74 72 62 61 56 42
40 9
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1000 1153 1367 1669 2050 1000
Size: 153 214 302 381 3053
Fragments arranged by size:
3053 381 302 214 153
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 281 550 772 2101 2282 2491 281
Size: 269 222 1329 181 209 1893
Fragments arranged by size:
1893 1329 269 222 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 123 560 1480 123
Size: 437 920 2746
Fragments arranged by size:
2746 920 437
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstYI r'GATC_y
Cuts at: 1 585 1128 1595 1618 1953 2199 3211 3297
Size: 584 543 467 23 335 246 1012 86
Cuts at: 3297 3309 3655 1
Size: 12 346 449
Fragments arranged by size:
1012 584 543 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 508 508
Size: 4103
BtgI C'CryG_G
Cuts at: 498 674 728 498
Size: 176 54 3873
Fragments arranged by size:
3873 176 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 162 365 2053 2234 2397 162
Size: 203 1688 181 163 1868
Fragments arranged by size:
1868 1688 203 181 163
BtsI GCAGTG_nn'
Cuts at: 565 970 1346 565
Size: 405 376 3322
Fragments arranged by size:
3322 405 376
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DraIII CAC_nnn'GTG
Cuts at: 499 499
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 532 1809 2667 532
Size: 1277 858 1968
Fragments arranged by size:
1968 1277 858
EaeI y'GGCC_r
Cuts at: 194 667 731 1688 1862 2253 2280 3625 194
Size: 473 64 957 174 391 27 1345 672
Fragments arranged by size:
1345 957 672 473 391 174 64 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 2311 2621 2767 3913 295
Size: 2016 310 146 1146 485
Fragments arranged by size:
2016 1146 485 310 146
EcoICRI GAG'CTC
Cuts at: 206 723 1115 206
Size: 517 392 3194
Fragments arranged by size:
3194 517 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1928 2360 3107 3375 935
Size: 993 432 747 268 1663
Fragments arranged by size:
1663 993 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 480 481 525 594 804 966 1242 480
Size: 1 44 69 210 162 276 3341
Fragments arranged by size:
3341 276 210 162 69 44 1
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 140 1537 1785 2807 3786 140
Size: 1397 248 1022 979 457
Fragments arranged by size:
1397 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1945 1975 1977 2007 1945
Size: 30 2 30 4041
Fragments arranged by size:
4041 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
HindIII A'AGCT_T
Cuts at: 600 600
Size: 4103
KasI G'GCGC_C
Cuts at: 136 1533 1781 136
Size: 1397 248 2458
Fragments arranged by size:
2458 1397 248
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 196 733 1864 196
Size: 537 1131 2435
Fragments arranged by size:
2435 1131 537
MslI CAynn'nnrTG
Cuts at: 2092 2219 2092
Size: 127 3976
MspA1I CmG'CkG
Cuts at: 219 1214 1528 1888 2901 3146 219
Size: 995 314 360 1013 245 1176
Fragments arranged by size:
1176 1013 995 360 314 245
NaeI GCC'GGC
Cuts at: 774 2284 774
Size: 1510 2593
NarI GG'CG_CC
Cuts at: 137 1534 1782 137
Size: 1397 248 2458
Fragments arranged by size:
2458 1397 248
NcoI C'CATG_G
Cuts at: 674 674
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 772 2282 772
Size: 1510 2593
NspI r_CATG'y
Cuts at: 411 713 1156 1656 2563 411
Size: 302 443 500 907 1951
Fragments arranged by size:
1951 907 500 443 302
PasI CC'CwG_GG
Cuts at: 715 1001 715
Size: 286 3817
PciI A'CATG_T
Cuts at: 407 2559 407
Size: 2152 1951
PflMI CCAn_nnn'nTGG
Cuts at: 760 760
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 525 525
Size: 4103
PspOMI G'GGCC_C
Cuts at: 480 965 480
Size: 485 3618
PstI C_TGCA'G
Cuts at: 866 1150 1835 866
Size: 284 685 3134
Fragments arranged by size:
3134 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 208 725 1117 208
Size: 517 392 3194
Fragments arranged by size:
3194 517 392
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 168 862 1146 1831 2824 3015 33
Size: 135 694 284 685 993 191 1121
Fragments arranged by size:
1121 993 694 685 284 191 135
SfoI GGC'GCC
Cuts at: 138 1535 1783 138
Size: 1397 248 2458
Fragments arranged by size:
2458 1397 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 129 478 1125 129
Size: 349 647 3107
Fragments arranged by size:
3107 647 349
SmlI C'TyrA_G
Cuts at: 901 1258 2173 2665 2927 901
Size: 357 915 492 262 2077
Fragments arranged by size:
2077 915 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 713 1156 1656 713
Size: 443 500 3160
Fragments arranged by size:
3160 500 443
SrfI GCCC'GGGC
Cuts at: 129 129
Size: 4103
StuI AGG'CCT
Cuts at: 663 899 663
Size: 236 3867
StyI C'CwwG_G
Cuts at: 488 590 674 1237 1591 488
Size: 102 84 563 354 3000
Fragments arranged by size:
3000 563 354 102 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 687 957 1686 1819 2278 2487 687
Size: 270 729 133 459 209 2303
Fragments arranged by size:
2303 729 459 270 209 133
TatI w'GTAC_w
Cuts at: 3618 3618
Size: 4103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 127 476 1123 127
Size: 349 647 3107
Fragments arranged by size:
3107 647 349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI XhoI XmnI ZraI