Restriction map for
pF1KB9590
(Circular) (Six-base) MAPSORT of: pF1KB9590.seq Check: 8904 from: 1 to: 4328
pF1KB9590 4328 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1366 1366
Size: 4328
Acc65I G'GTAC_C
Cuts at: 1344 1344
Size: 4328
AclI AA'CG_TT
Cuts at: 4168 4168
Size: 4328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 2153 2585 3332 3600 152
Size: 2001 432 747 268 880
Fragments arranged by size:
2001 880 747 432 268
AflIII A'CryG_T
Cuts at: 1680 2784 3761 1680
Size: 1104 977 2247
Fragments arranged by size:
2247 1104 977
AgeI A'CCGG_T
Cuts at: 2716 2716
Size: 4328
AleI CACnn'nnGTG
Cuts at: 985 985
Size: 4328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 358
Size: 32 4296
AlwNI CAG_nnn'CTG
Cuts at: 1633 3200 1633
Size: 1567 2761
ApaLI G'TGCA_C
Cuts at: 3098 3098
Size: 4328
ApoI r'AATT_y
Cuts at: 1062 1332 3681 3937 1062
Size: 270 2349 256 1453
Fragments arranged by size:
2349 1453 270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4328
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
AvaI C'yCGr_G
Cuts at: 217 1348 217
Size: 1131 3197
BamHI G'GATC_C
Cuts at: 1353 1353
Size: 4328
BanI G'GyrC_C
Cuts at: 1344 1758 2006 2041 1344
Size: 414 248 35 3631
Fragments arranged by size:
3631 414 248 35
BanII G_rGCy'C
Cuts at: 1342 2372 1342
Size: 1030 3298
BbeI G_GCGC'C
Cuts at: 1762 2010 1762
Size: 248 4080
BbsI GAAGACnn'nnnn_
Cuts at: 904 1049 904
Size: 145 4183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1182 1216 1182
Size: 34 4294
BciVI GTATCCnnnnn_n'
Cuts at: 2226 2987 4328 2226
Size: 761 1341 2226
Fragments arranged by size:
2226 1341 761
BclI T'GATC_A
Cuts at: 1848 1848
Size: 4328
BglII A'GATC_T
Cuts at: 1843 3880 1843
Size: 2037 2291
BlpI GC'TnA_GC
Cuts at: 1440 1440
Size: 4328
Bme1580I G_kGCm'C
Cuts at: 872 1953 2046 3102 872
Size: 1081 93 1056 2098
Fragments arranged by size:
2098 1081 1056 93
BmgBI CAC'GTC
Cuts at: 576 576
Size: 4328
BmrI ACTGGGnnnn_n'
Cuts at: 1540 1621 1956 4100 1540
Size: 81 335 2144 1768
Fragments arranged by size:
2144 1768 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 4328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 1102 1224 624
Size: 478 122 3728
Fragments arranged by size:
3728 478 122
Bpu10I CC'TnA_GC
Cuts at: 1106 1106
Size: 4328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1504 2383 2875 3173 133
Size: 1371 879 492 298 1288
Fragments arranged by size:
1371 1288 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 291 1243 4032 291
Size: 952 2789 587
Fragments arranged by size:
2789 952 587
BsaAI yAC'GTr
Cuts at: 867 1092 1160 1681 867
Size: 225 68 521 3514
Fragments arranged by size:
3514 521 225 68
BsaBI GATnn'nnATC
Cuts at: 6 1842 3879 6
Size: 1836 2037 455
Fragments arranged by size:
2037 1836 455
BsaHI Gr'CG_yC
Cuts at: 1759 2007 2709 4205 1759
Size: 248 702 1496 1882
Fragments arranged by size:
1882 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1216 1517 2038 2716 2990 3137 3548 1216
Size: 301 521 678 274 147 411 1996
Fragments arranged by size:
1996 678 521 411 301 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1222 1252 1222
Size: 30 4298
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 372 838 1279 1288 1291 372
Size: 466 441 9 3 3409
Fragments arranged by size:
3409 466 441 9 3
BseYI C'CCAG_C
Cuts at: 343 1254 1753 2511 3088 3584 343
Size: 911 499 758 577 496 1087
Fragments arranged by size:
1087 911 758 577 499 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 4328
BsiEI CG_ry'CG
Cuts at: 104 844 1870 1916 3124 104
Size: 740 1026 46 1208 1308
Fragments arranged by size:
1308 1208 1026 740 46
BsiHKAI G_wGCw'C
Cuts at: 1342 2120 2310 3102 1342
Size: 778 190 792 2568
Fragments arranged by size:
2568 792 778 190
BsiWI C'GTAC_G
Cuts at: 1088 1088
Size: 4328
BsmI GAATG_Cn'
Cuts at: 372 372
Size: 4328
BsmBI CGTCTCn'nnnn_
Cuts at: 460 460
Size: 4328
Bsp1286I G_dGCh'C
Cuts at: 872 1342 1953 2046 2120 2310 2372 3102 872
Size: 470 611 93 74 190 62 730 2098
Fragments arranged by size:
2098 730 611 470 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3548 3548
Size: 4328
BspHI T'CATG_A
Cuts at: 3504 3504
Size: 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 819 1129 1378 1592 1894 2275 819
Size: 310 249 214 302 381 2872
Fragments arranged by size:
2872 381 310 302 249 214
BsrBI CCG'CTC
Cuts at: 50 216 2620 2674 4158 4323 50
Size: 166 2404 54 1484 165 55
Fragments arranged by size:
2404 1484 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1187 2240 1187
Size: 1053 3275
BsrFI r'CCGG_y
Cuts at: 2326 2507 2716 2326
Size: 181 209 3938
Fragments arranged by size:
3938 209 181
BssSI C'ACGA_G
Cuts at: 2599 2957 2599
Size: 358 3970
BstAPI GCAn_nnn'nTGC
Cuts at: 231 1705 231
Size: 1474 2854
BstBI TT'CG_AA
Cuts at: 2689 2689
Size: 4328
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1065
Size: 4328
BstYI r'GATC_y
Cuts at: 1 1222 1353 1820 1843 2178 2424 3436 3522
Size: 1221 131 467 23 335 246 1012 86
Cuts at: 3522 3534 3880 1
Size: 12 346 449
Fragments arranged by size:
1221 1012 467 449 346 335 246 131
86 23 12
BtgI C'CryG_G
Cuts at: 107 185 107
Size: 78 4250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2278 2459 2622 2278
Size: 181 163 3984
Fragments arranged by size:
3984 181 163
BtsI GCAGTG_nn'
Cuts at: 1571 1571
Size: 4328
DraI TTT'AAA
Cuts at: 1327 1327
Size: 4328
DrdI GACnn_nn'nnGTC
Cuts at: 2034 2892 2034
Size: 858 3470
EaeI y'GGCC_r
Cuts at: 188 623 841 1055 1205 1913 2087 2478 2505
Size: 435 218 214 150 708 174 391 27
Cuts at: 2505 3850 188
Size: 1345 666
Fragments arranged by size:
1345 708 666 435 391 218 214 174
150 27
EagI C'GGCC_G
Cuts at: 841 1913 841
Size: 1072 3256
EarI CTCTTCn'nnn_
Cuts at: 718 1265 2351 2561 718
Size: 547 1086 210 2485
Fragments arranged by size:
2485 1086 547 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2536 2846 2992 4138 2536
Size: 310 146 1146 2726
Fragments arranged by size:
2726 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1340 1340
Size: 4328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 624 1102 1224 2153 2585 3332 3600 152
Size: 472 478 122 929 432 747 268 880
Fragments arranged by size:
929 880 747 478 472 432 268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 323 323
Size: 4328
EcoO109I rG'GnC_Cy
Cuts at: 1467 1467
Size: 4328
EcoRI G'AATT_C
Cuts at: 1332 1332
Size: 4328
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 246 2347 246
Size: 2101 2227
FspI TGC'GCA
Cuts at: 2109 2109
Size: 4328
HaeII r_GCGC'y
Cuts at: 1762 2010 3032 4011 1762
Size: 248 1022 979 2079
Fragments arranged by size:
2079 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 916 948 2170 2200 2202 2232 916
Size: 32 1222 30 2 30 3012
Fragments arranged by size:
3012 1222 32 30 30 2
HincII GTy'rAC
Cuts at: 261 1367 261
Size: 1106 3222
KasI G'GCGC_C
Cuts at: 1758 2006 1758
Size: 248 4080
KpnI G_GTAC'C
Cuts at: 1348 1348
Size: 4328
MluI A'CGCG_T
Cuts at: 3761 3761
Size: 4328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 947 2999 3183 947
Size: 2052 184 2092
Fragments arranged by size:
2092 2052 184
MscI TGG'CCA
Cuts at: 625 1057 1207 2089 625
Size: 432 150 882 2864
Fragments arranged by size:
2864 882 432 150
MslI CAynn'nnrTG
Cuts at: 319 865 985 2317 2444 319
Size: 546 120 1332 127 2203
Fragments arranged by size:
2203 1332 546 127 120
MspA1I CmG'CkG
Cuts at: 840 846 1439 1753 2113 3126 3371 840
Size: 6 593 314 360 1013 245 1797
Fragments arranged by size:
1797 1013 593 360 314 245 6
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 4328
NarI GG'CG_CC
Cuts at: 1759 2007 1759
Size: 248 4080
NcoI C'CATG_G
Cuts at: 107 107
Size: 4328
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 4328
NotI GC'GGCC_GC
Cuts at: 841 841
Size: 4328
NspI r_CATG'y
Cuts at: 1381 1881 2788 1381
Size: 500 907 2921
Fragments arranged by size:
2921 907 500
PciI A'CATG_T
Cuts at: 2784 2784
Size: 4328
PflMI CCAn_nnn'nTGG
Cuts at: 276 504 819 276
Size: 228 315 3785
Fragments arranged by size:
3785 315 228
PfoI T'CCnGG_A
Cuts at: 1024 1024
Size: 4328
PmeI GTTT'AAAC
Cuts at: 1327 1327
Size: 4328
PmlI CAC'GTG
Cuts at: 867 1160 867
Size: 293 4035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 3467
Size: 32 4296
PstI C_TGCA'G
Cuts at: 916 1375 2060 916
Size: 459 685 3184
Fragments arranged by size:
3184 685 459
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4328
PvuII CAG'CTG
Cuts at: 1753 2113 1753
Size: 360 3968
RsrII CG'GwC_CG
Cuts at: 2523 2523
Size: 4328
SacI G_AGCT'C
Cuts at: 1342 1342
Size: 4328
SalI G'TCGA_C
Cuts at: 1365 1365
Size: 4328
SapI GCTCTTCn'nnn_
Cuts at: 2351 2561 2351
Size: 210 4118
SbfI CC_TGCA'GG
Cuts at: 1375 1375
Size: 4328
ScaI AGT'ACT
Cuts at: 3845 3845
Size: 4328
SexAI A'CCwGG_T
Cuts at: 797 797
Size: 4328
SfcI C'TryA_G
Cuts at: 33 775 912 1371 2056 3049 3240 33
Size: 742 137 459 685 993 191 1121
Fragments arranged by size:
1121 993 742 685 459 191 137
SfoI GGC'GCC
Cuts at: 1760 2008 1760
Size: 248 4080
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
SmaI CCC'GGG
Cuts at: 1350 1350
Size: 4328
SmlI C'TyrA_G
Cuts at: 148 1483 2398 2890 3152 148
Size: 1335 915 492 262 1324
Fragments arranged by size:
1335 1324 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4328
SphI G_CATG'C
Cuts at: 1381 1881 1381
Size: 500 3828
StyI C'CwwG_G
Cuts at: 107 391 1462 1816 107
Size: 284 1071 354 2619
Fragments arranged by size:
2619 1071 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 1911 2044 2503 2712 357
Size: 1554 133 459 209 1973
Fragments arranged by size:
1973 1554 459 209 133
TatI w'GTAC_w
Cuts at: 1229 3843 1229
Size: 2614 1714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 4296
Tth111I GACn'n_nGTC
Cuts at: 2125 2125
Size: 4328
XbaI T'CTAG_A
Cuts at: 1359 1359
Size: 4328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1823 183
Size: 1640 2688
XmaI C'CCGG_G
Cuts at: 1348 1348
Size: 4328
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PflMI PfoI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI