Restriction map for
pF1KB9588
(Circular) (Six-base) MAPSORT of: pF1KB9588.seq Check: 8753 from: 1 to: 3812
pF1KB9588 3812 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2069 2816 3084 1637
Size: 432 747 268 2365
Fragments arranged by size:
2365 747 432 268
AflII C'TTAA_G
Cuts at: 442 442
Size: 3812
AflIII A'CryG_T
Cuts at: 459 1164 2268 3245 459
Size: 705 1104 977 1026
Fragments arranged by size:
1104 1026 977 705
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 146 146
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 1117 2684 1117
Size: 1567 2245
ApaLI G'TGCA_C
Cuts at: 131 2582 131
Size: 2451 1361
ApoI r'AATT_y
Cuts at: 726 816 3165 3421 726
Size: 90 2349 256 1117
Fragments arranged by size:
2349 1117 256 90
AseI AT'TA_AT
Cuts at: 20 368 20
Size: 348 3464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 832 832
Size: 3812
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 828 1242 1490 1525 828
Size: 414 248 35 3115
Fragments arranged by size:
3115 414 248 35
BanII G_rGCy'C
Cuts at: 406 826 1856 406
Size: 420 1030 2362
Fragments arranged by size:
2362 1030 420
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 324 1332 324
Size: 1008 2804
BglII A'GATC_T
Cuts at: 438 1327 3364 438
Size: 889 2037 886
Fragments arranged by size:
2037 889 886
BlpI GC'TnA_GC
Cuts at: 195 924 195
Size: 729 3083
Bme1580I G_kGCm'C
Cuts at: 135 1437 1530 2586 135
Size: 1302 93 1056 1361
Fragments arranged by size:
1361 1302 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 988 1867 2359 2657 531
Size: 457 879 492 298 1686
Fragments arranged by size:
1686 879 492 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 499 3516 499
Size: 3017 795
BsaAI yAC'GTr
Cuts at: 462 1165 462
Size: 703 3109
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 1243 1491 2193 3689 1243
Size: 248 702 1496 1366
Fragments arranged by size:
1496 1366 702 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 135 826 1604 1794 2586 135
Size: 691 778 190 792 1361
Fragments arranged by size:
1361 792 778 691 190
Bsp1286I G_dGCh'C
Cuts at: 135 406 826 1437 1530 1604 1794 1856 2586
Size: 271 420 611 93 74 190 62 730
Cuts at: 2586 135
Size: 1361
Fragments arranged by size:
1361 730 611 420 271 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 518 2988 518
Size: 2470 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1076 1378 1759 862
Size: 214 302 381 2915
Fragments arranged by size:
2915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BsrGI T'GTAC_A
Cuts at: 456 456
Size: 3812
BssSI C'ACGA_G
Cuts at: 328 2083 2441 328
Size: 1755 358 1699
Fragments arranged by size:
1755 1699 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 438 837 1304 1327 1662 1908 2920 3006
Size: 437 399 467 23 335 246 1012 86
Cuts at: 3006 3018 3364 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 437 399 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 1397 1571 1962 1989 3334 1397
Size: 174 391 27 1345 1875
Fragments arranged by size:
1875 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 419 1835 2045 419
Size: 1416 210 2186
Fragments arranged by size:
2186 1416 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 2020 2330 2476 3622 265
Size: 1755 310 146 1146 455
Fragments arranged by size:
1755 1146 455 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2069 2816 3084 1637
Size: 432 747 268 2365
Fragments arranged by size:
2365 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 951 951
Size: 3812
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 1246 1494 2516 3495 1246
Size: 248 1022 979 1563
Fragments arranged by size:
1563 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1654 1684 1686 1716 1654
Size: 30 2 30 3750
Fragments arranged by size:
3750 30 30 2
HincII GTy'rAC
Cuts at: 541 851 541
Size: 310 3502
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MfeI C'AATT_G
Cuts at: 693 693
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 1573 1573
Size: 3812
MslI CAynn'nnrTG
Cuts at: 1801 1928 1801
Size: 127 3685
MspA1I CmG'CkG
Cuts at: 556 923 1237 1597 2610 2855 556
Size: 367 314 360 1013 245 1513
Fragments arranged by size:
1513 1013 367 360 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NcoI C'CATG_G
Cuts at: 107 107
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PasI CC'CwG_GG
Cuts at: 406 406
Size: 3812
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PmlI CAC'GTG
Cuts at: 462 462
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PsiI TTA'TAA
Cuts at: 475 475
Size: 3812
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 556 1237 1597 556
Size: 681 360 2771
Fragments arranged by size:
2771 681 360
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 855 1540 2533 2724 33
Size: 822 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 191
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 442 510 967 1882 2374 2636 442
Size: 68 457 915 492 262 1618
Fragments arranged by size:
1618 915 492 457 262 68
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
SspI AAT'ATT
Cuts at: 183 183
Size: 3812
StyI C'CwwG_G
Cuts at: 107 946 1300 107
Size: 839 354 2619
Fragments arranged by size:
2619 839 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 1395 1528 1987 2196 100
Size: 1295 133 459 209 1716
Fragments arranged by size:
1716 1295 459 209 133
TatI w'GTAC_w
Cuts at: 456 697 3327 456
Size: 241 2630 941
Fragments arranged by size:
2630 941 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
XmnI GAAnn'nnTTC
Cuts at: 182 305 596 182
Size: 123 291 3398
Fragments arranged by size:
3398 291 123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI