(Circular) (Six-base) MAPSORT of: pF1KB9586.seq  Check: 4646  from: 1  to: 3563



pF1KB9586  3563 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AccI GT'mk_AC

Cuts at:    601     601 
   Size:       3563
 
Acc65I G'GTAC_C

Cuts at:    579     579 
   Size:       3563
 
AclI AA'CG_TT

Cuts at:   3403    3403 
   Size:       3563
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1388    1820    2567    2835    1388 
   Size:        432     747     268    2116

  Fragments arranged by size: 

                2116     747     432     268
 
AflIII A'CryG_T

Cuts at:    915    2019    2996     915 
   Size:       1104     977    1482

  Fragments arranged by size: 

                1482    1104     977
 
AgeI A'CCGG_T

Cuts at:   1951    1951 
   Size:       3563
 
AlwNI CAG_nnn'CTG

Cuts at:    868    2435     868 
   Size:       1567    1996
 
ApaLI G'TGCA_C

Cuts at:   2333    2333 
   Size:       3563
 
ApoI r'AATT_y

Cuts at:    567    2916    3172     567 
   Size:       2349     256     958

  Fragments arranged by size: 

                2349     958     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3563
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3563
 
AvaI C'yCGr_G

Cuts at:    583     583 
   Size:       3563
 
BamHI G'GATC_C

Cuts at:    588     588 
   Size:       3563
 
BanI G'GyrC_C

Cuts at:    210     579     993    1241    1276     210 
   Size:        369     414     248      35    2497

  Fragments arranged by size: 

                2497     414     369     248      35
 
BanII G_rGCy'C

Cuts at:    577    1607     577 
   Size:       1030    2533
 
BbeI G_GCGC'C

Cuts at:    997    1245     997 
   Size:        248    3315
 
BbvCI CC'TCA_GC

Cuts at:    203     203 
   Size:       3563
 
BciVI GTATCCnnnnn_n'

Cuts at:   1461    2222    3563    1461 
   Size:        761    1341    1461

  Fragments arranged by size: 

                1461    1341     761
 
BclI T'GATC_A

Cuts at:   1083    1083 
   Size:       3563
 
BglII A'GATC_T

Cuts at:   1078    3115    1078 
   Size:       2037    1526
 
BlpI GC'TnA_GC

Cuts at:    675     675 
   Size:       3563
 
Bme1580I G_kGCm'C

Cuts at:   1188    1281    2337    1188 
   Size:         93    1056    2414

  Fragments arranged by size: 

                2414    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    775     856    1191    3335     775 
   Size:         81     335    2144    1003

  Fragments arranged by size: 

                2144    1003     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1930    1930 
   Size:       3563
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    122     122 
   Size:       3563
 
Bpu10I CC'TnA_GC

Cuts at:    203     203 
   Size:       3563
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    496     739    1618    2110    2408     496 
   Size:        243     879     492     298    1651

  Fragments arranged by size: 

                1651     879     492     298     243
 
BsaI GGTCTCn'nnnn_

Cuts at:   3267    3267 
   Size:       3563
 
BsaAI yAC'GTr

Cuts at:    916     916 
   Size:       3563
 
BsaBI GATnn'nnATC

Cuts at:      6    1077    3114       6 
   Size:       1071    2037     455

  Fragments arranged by size: 

                2037    1071     455
 
BsaHI Gr'CG_yC

Cuts at:    994    1242    1944    3440     994 
   Size:        248     702    1496    1117

  Fragments arranged by size: 

                1496    1117     702     248
 
BsaWI w'CCGG_w

Cuts at:    752    1273    1951    2225    2372    2783     752 
   Size:        521     678     274     147     411    1532

  Fragments arranged by size: 

                1532     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    245     245 
   Size:       3563
 
BseYI C'CCAG_C

Cuts at:    250     988    1746    2323    2819     250 
   Size:        738     758     577     496     994

  Fragments arranged by size: 

                 994     758     738     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1105    1151    2359     104 
   Size:       1001      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1001      46
 
BsiHKAI G_wGCw'C

Cuts at:    577    1355    1545    2337     577 
   Size:        778     190     792    1803

  Fragments arranged by size: 

                1803     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    577    1188    1281    1355    1545    1607    2337     577 
   Size:        611      93      74     190      62     730    1803

  Fragments arranged by size: 

                1803     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2783    2783 
   Size:       3563
 
BspHI T'CATG_A

Cuts at:   2739    2739 
   Size:       3563
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    186     613     827    1129    1510     186 
   Size:        427     214     302     381    2239

  Fragments arranged by size: 

                2239     427     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1855    1909    3393    3558      50 
   Size:       1805      54    1484     165      55

  Fragments arranged by size: 

                1805    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1475    1475 
   Size:       3563
 
BsrFI r'CCGG_y

Cuts at:    344    1561    1742    1951     344 
   Size:       1217     181     209    1956

  Fragments arranged by size: 

                1956    1217     209     181
 
BssSI C'ACGA_G

Cuts at:   1834    2192    1834 
   Size:        358    3205
 
BstAPI GCAn_nnn'nTGC

Cuts at:    940     940 
   Size:       3563
 
BstBI TT'CG_AA

Cuts at:   1924    1924 
   Size:       3563
 
BstYI r'GATC_y

Cuts at:      1     588    1055    1078    1413    1659    2671    2757    2769 
   Size:        587     467      23     335     246    1012      86      12

Cuts at:   2769    3115       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     587     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    278     278 
   Size:       3563
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1513    1694    1857    1513 
   Size:        181     163    3219

  Fragments arranged by size: 

                3219     181     163
 
BtsI GCAGTG_nn'

Cuts at:    376     806     376 
   Size:        430    3133
 
DraI TTT'AAA

Cuts at:    562     562 
   Size:       3563
 
DrdI GACnn_nn'nnGTC

Cuts at:   1269    2127    1269 
   Size:        858    2705
 
EaeI y'GGCC_r

Cuts at:   1148    1322    1713    1740    3085    1148 
   Size:        174     391      27    1345    1626

  Fragments arranged by size: 

                1626    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1148    1148 
   Size:       3563
 
EarI CTCTTCn'nnn_

Cuts at:    398    1586    1796     398 
   Size:       1188     210    2165

  Fragments arranged by size: 

                2165    1188     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1771    2081    2227    3373    1771 
   Size:        310     146    1146    1961

  Fragments arranged by size: 

                1961    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    575     575 
   Size:       3563
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    122    1388    1820    2567    2835     122 
   Size:       1266     432     747     268     850

  Fragments arranged by size: 

                1266     850     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    227     227 
   Size:       3563
 
EcoO109I rG'GnC_Cy

Cuts at:    702     702 
   Size:       3563
 
EcoRI G'AATT_C

Cuts at:    567     567 
   Size:       3563
 
EcoRV GAT'ATC

Cuts at:    119     119 
   Size:       3563
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1582    1582 
   Size:       3563
 
FspI TGC'GCA

Cuts at:   1344    1344 
   Size:       3563
 
HaeII r_GCGC'y

Cuts at:    997    1245    2267    3246     997 
   Size:        248    1022     979    1314

  Fragments arranged by size: 

                1314    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    375     407    1405    1435    1437    1467     375 
   Size:         32     998      30       2      30    2471

  Fragments arranged by size: 

                2471     998      32      30      30       2
 
HincII GTy'rAC

Cuts at:    602     602 
   Size:       3563
 
KasI G'GCGC_C

Cuts at:    993    1241     993 
   Size:        248    3315
 
KpnI G_GTAC'C

Cuts at:    583     583 
   Size:       3563
 
MluI A'CGCG_T

Cuts at:   2996    2996 
   Size:       3563
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    398    2234    2418     398 
   Size:       1836     184    1543

  Fragments arranged by size: 

                1836    1543     184
 
MscI TGG'CCA

Cuts at:   1324    1324 
   Size:       3563
 
MslI CAynn'nnrTG

Cuts at:    331    1552    1679     331 
   Size:       1221     127    2215

  Fragments arranged by size: 

                2215    1221     127
 
MspA1I CmG'CkG

Cuts at:    185     207     674     988    1348    2361    2606     185 
   Size:         22     467     314     360    1013     245    1142

  Fragments arranged by size: 

                1142    1013     467     360     314     245      22
 
NaeI GCC'GGC

Cuts at:   1744    1744 
   Size:       3563
 
NarI GG'CG_CC

Cuts at:    994    1242     994 
   Size:        248    3315
 
NcoI C'CATG_G

Cuts at:    278     278 
   Size:       3563
 
NgoMIV G'CCGG_C

Cuts at:   1742    1742 
   Size:       3563
 
NspI r_CATG'y

Cuts at:    181     484     616    1116    2023     181 
   Size:        303     132     500     907    1721

  Fragments arranged by size: 

                1721     907     500     303     132
 
PasI CC'CwG_GG

Cuts at:    223     223 
   Size:       3563
 
PciI A'CATG_T

Cuts at:   2019    2019 
   Size:       3563
 
PmeI GTTT'AAAC

Cuts at:    562     562 
   Size:       3563
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2702    2734    2702 
   Size:         32    3531
 
PstI C_TGCA'G

Cuts at:    383     610    1295     383 
   Size:        227     685    2651

  Fragments arranged by size: 

                2651     685     227
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3563
 
PvuII CAG'CTG

Cuts at:    207     988    1348     207 
   Size:        781     360    2422

  Fragments arranged by size: 

                2422     781     360
 
RsrII CG'GwC_CG

Cuts at:   1758    1758 
   Size:       3563
 
SacI G_AGCT'C

Cuts at:    577     577 
   Size:       3563
 
SalI G'TCGA_C

Cuts at:    600     600 
   Size:       3563
 
SapI GCTCTTCn'nnn_

Cuts at:   1586    1796    1586 
   Size:        210    3353
 
SbfI CC_TGCA'GG

Cuts at:    610     610 
   Size:       3563
 
ScaI AGT'ACT

Cuts at:   3080    3080 
   Size:       3563
 
SfcI C'TryA_G

Cuts at:     33     379     606    1291    2284    2475      33 
   Size:        346     227     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     346     227     191
 
SfoI GGC'GCC

Cuts at:    995    1243     995 
   Size:        248    3315
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3563
 
SmaI CCC'GGG

Cuts at:    585     585 
   Size:       3563
 
SmlI C'TyrA_G

Cuts at:    475     718    1633    2125    2387     475 
   Size:        243     915     492     262    1651

  Fragments arranged by size: 

                1651     915     492     262     243
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3563
 
SphI G_CATG'C

Cuts at:    484     616    1116     484 
   Size:        132     500    2931

  Fragments arranged by size: 

                2931     500     132
 
StyI C'CwwG_G

Cuts at:    278     443     697    1051     278 
   Size:        165     254     354    2790

  Fragments arranged by size: 

                2790     354     254     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1146    1279    1738    1947    1146 
   Size:        133     459     209    2762

  Fragments arranged by size: 

                2762     459     209     133
 
TatI w'GTAC_w

Cuts at:    456    3078     456 
   Size:       2622     941
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3080    3112    3080 
   Size:         32    3531
 
Tth111I GACn'n_nGTC

Cuts at:   1360    1360 
   Size:       3563
 
XbaI T'CTAG_A

Cuts at:    594     594 
   Size:       3563
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1058    1058 
   Size:       3563
 
XmaI C'CCGG_G

Cuts at:    583     583 
   Size:       3563
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI