Restriction map for
pF1KB9581
(Circular) (Six-base) MAPSORT of: pF1KB9581.seq Check: 9178 from: 1 to: 4175
pF1KB9581 4175 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 228 228
Size: 4175
AccI GT'mk_AC
Cuts at: 251 518 1213 251
Size: 267 695 3213
Fragments arranged by size:
3213 695 267
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 2000 2432 3179 3447 161
Size: 1839 432 747 268 889
Fragments arranged by size:
1839 889 747 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 949 1480 3047 949
Size: 531 1567 2077
Fragments arranged by size:
2077 1567 531
ApaI G_GGCC'C
Cuts at: 549 549
Size: 4175
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1035 1126 1179 3528 3784 1035
Size: 91 53 2349 256 1426
Fragments arranged by size:
2349 1426 256 91 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 392 463 913 1054 1195 392
Size: 71 450 141 141 3372
Fragments arranged by size:
3372 450 141 141 71
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 370 442 1191 1605 1853 1888 370
Size: 72 749 414 248 35 2657
Fragments arranged by size:
2657 749 414 248 72 35
BanII G_rGCy'C
Cuts at: 463 549 876 1189 2219 463
Size: 86 327 313 1030 2419
Fragments arranged by size:
2419 1030 327 313 86
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 624 624
Size: 4175
BglI GCCn_nnn'nGGC
Cuts at: 301 379 301
Size: 78 4097
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 875 1287 875
Size: 412 3763
Bme1580I G_kGCm'C
Cuts at: 549 1800 1893 2949 549
Size: 1251 93 1056 1775
Fragments arranged by size:
1775 1251 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 336 1387 1468 1803 3947 336
Size: 1051 81 335 2144 564
Fragments arranged by size:
2144 1051 564 335 81
BmtI G_CTAG'C
Cuts at: 595 595
Size: 4175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 773
Size: 4175
Bpu10I CC'TnA_GC
Cuts at: 260 260
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 152 697 3879 152
Size: 545 3182 448
Fragments arranged by size:
3182 545 448
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 886 1364 1885 2563 2837 2984 3395 886
Size: 478 521 678 274 147 411 1666
Fragments arranged by size:
1666 678 521 478 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 444 444
Size: 4175
BseYI C'CCAG_C
Cuts at: 176 366 525 760 868 942 1600 2358 2935
Size: 190 159 235 108 74 658 758 577
Cuts at: 2935 3431 176
Size: 496 920
Fragments arranged by size:
920 758 658 577 496 235 190 159
108 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 595 967 988 1096 309
Size: 286 372 21 108 3388
Fragments arranged by size:
3388 372 286 108 21
BsiEI CG_ry'CG
Cuts at: 104 411 863 904 1717 1763 2971 104
Size: 307 452 41 813 46 1208 1308
Fragments arranged by size:
1308 1208 813 452 307 46 41
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 579 579
Size: 4175
Bsp1286I G_dGCh'C
Cuts at: 463 549 876 1189 1800 1893 1967 2157 2219
Size: 86 327 313 611 93 74 190 62
Cuts at: 2219 2949 463
Size: 730 1689
Fragments arranged by size:
1689 730 611 327 313 190 93 86
74 62
BspEI T'CCGG_A
Cuts at: 886 3395 886
Size: 2509 1666
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 1225 1439 1741 2122 228
Size: 997 214 302 381 2281
Fragments arranged by size:
2281 997 381 302 214
BsrBI CCG'CTC
Cuts at: 50 612 2467 2521 4005 4170 50
Size: 562 1855 54 1484 165 55
Fragments arranged by size:
1855 1484 562 165 55 54
BsrDI GCAATG_nn'
Cuts at: 663 2087 663
Size: 1424 2751
BsrFI r'CCGG_y
Cuts at: 468 2173 2354 2563 468
Size: 1705 181 209 2080
Fragments arranged by size:
2080 1705 209 181
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 1074 1552 1074
Size: 478 3697
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 296 296
Size: 4175
BstYI r'GATC_y
Cuts at: 1 1200 1667 1690 2025 2271 3283 3369 3381
Size: 1199 467 23 335 246 1012 86 12
Cuts at: 3381 3727 1
Size: 346 449
Fragments arranged by size:
1199 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 827 827
Size: 4175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 151 1033 2125 2306 2469 151
Size: 882 1092 181 163 1857
Fragments arranged by size:
1857 1092 882 181 163
BtsI GCAGTG_nn'
Cuts at: 1418 1418
Size: 4175
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 408 901 1760 1934 2325 2352 3697 408
Size: 493 859 174 391 27 1345 886
Fragments arranged by size:
1345 886 859 493 391 174 27
EagI C'GGCC_G
Cuts at: 408 901 1760 408
Size: 493 859 2823
Fragments arranged by size:
2823 859 493
EarI CTCTTCn'nnn_
Cuts at: 125 2198 2408 125
Size: 2073 210 1892
Fragments arranged by size:
2073 1892 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 773 2000 2432 3179 3447 161
Size: 612 1227 432 747 268 889
Fragments arranged by size:
1227 889 747 612 432 268
EcoO109I rG'GnC_Cy
Cuts at: 546 1314 546
Size: 768 3407
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 655 1956 655
Size: 1301 2874
HaeII r_GCGC'y
Cuts at: 1010 1609 1857 2879 3858 1010
Size: 599 248 1022 979 1327
Fragments arranged by size:
1327 1022 979 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 2017 2047 2049 2079 228
Size: 32 1757 30 2 30 2324
Fragments arranged by size:
2324 1757 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 1148 2164 2291 1148
Size: 1016 127 3032
Fragments arranged by size:
3032 1016 127
MspA1I CmG'CkG
Cuts at: 385 437 489 651 764 829 868 900 972
Size: 52 52 162 113 65 39 32 72
Cuts at: 972 1286 1600 1960 2973 3218 385
Size: 314 314 360 1013 245 1342
Fragments arranged by size:
1342 1013 360 314 314 245 162 113
72 65 52 52 39 32
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NheI G'CTAG_C
Cuts at: 591 591
Size: 4175
NsiI A_TGCA'T
Cuts at: 626 626
Size: 4175
NspI r_CATG'y
Cuts at: 127 1228 1728 2635 127
Size: 1101 500 907 1667
Fragments arranged by size:
1667 1101 907 500
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 4175
PstI C_TGCA'G
Cuts at: 535 713 1222 1907 535
Size: 178 509 685 2803
Fragments arranged by size:
2803 685 509 178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 489 651 868 972 1600 1960 489
Size: 162 217 104 628 360 2704
Fragments arranged by size:
2704 628 360 217 162 104
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SacII CC_GC'GG
Cuts at: 830 830
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 531 709 1218 1903 2896 3087 33
Size: 498 178 509 685 993 191 1121
Fragments arranged by size:
1121 993 685 509 498 191 178
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 394 465 915 1056 1197 394
Size: 71 450 141 141 3372
Fragments arranged by size:
3372 450 141 141 71
SmlI C'TyrA_G
Cuts at: 1330 2245 2737 2999 1330
Size: 915 492 262 2506
Fragments arranged by size:
2506 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 127 1228 1728 127
Size: 1101 500 2574
Fragments arranged by size:
2574 1101 500
StyI C'CwwG_G
Cuts at: 1309 1663 1309
Size: 354 3821
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1094 1758 1891 2350 2559 358
Size: 736 664 133 459 209 1974
Fragments arranged by size:
1974 736 664 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 3692 3724 475
Size: 32 3185 32 926
Fragments arranged by size:
3185 926 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XmaI C'CCGG_G
Cuts at: 392 463 913 1054 1195 392
Size: 71 450 141 141 3372
Fragments arranged by size:
3372 450 141 141 71
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NsiI NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI