Restriction map for
pF1KB9578
(Circular) (Six-base) MAPSORT of: pF1KB9578.seq Check: 8073 from: 1 to: 3824
pF1KB9578 3824 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 1649 2081 2828 3096 408
Size: 1241 432 747 268 1136
Fragments arranged by size:
1241 1136 747 432 268
AfeI AGC'GCT
Cuts at: 379 379
Size: 3824
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AlwNI CAG_nnn'CTG
Cuts at: 627 630 1129 2696 627
Size: 3 499 1567 1755
Fragments arranged by size:
1755 1567 499 3
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 828 3177 3433 828
Size: 2349 256 1219
Fragments arranged by size:
2349 1219 256
AscI GG'CGCG_CC
Cuts at: 472 472
Size: 3824
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 781 844 781
Size: 63 3761
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 139 297 840 1254 1502 1537 139
Size: 158 543 414 248 35 2426
Fragments arranged by size:
2426 543 414 248 158 35
BanII G_rGCy'C
Cuts at: 763 838 1868 763
Size: 75 1030 2719
Fragments arranged by size:
2719 1030 75
BbeI G_GCGC'C
Cuts at: 143 301 1258 1506 143
Size: 158 957 248 2461
Fragments arranged by size:
2461 957 248 158
BbsI GAAGACnn'nnnn_
Cuts at: 377 377
Size: 3824
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglI GCCn_nnn'nGGC
Cuts at: 223 241 469 664 223
Size: 18 228 195 3383
Fragments arranged by size:
3383 228 195 18
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 775 936 775
Size: 161 3663
Bme1580I G_kGCm'C
Cuts at: 1449 1542 2598 1449
Size: 93 1056 2675
Fragments arranged by size:
2675 1056 93
BmgBI CAC'GTC
Cuts at: 543 543
Size: 3824
BmrI ACTGGGnnnn_n'
Cuts at: 1036 1117 1452 3596 1036
Size: 81 335 2144 1264
Fragments arranged by size:
2144 1264 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2371 2669 1000
Size: 879 492 298 2155
Fragments arranged by size:
2155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 303 741 3528 303
Size: 438 2787 599
Fragments arranged by size:
2787 599 438
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 140 298 671 1255 1503 2205 3701 140
Size: 158 373 584 248 702 1496 263
Fragments arranged by size:
1496 702 584 373 263 248 158
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2486 2633 3044 1013
Size: 521 678 274 147 411 1793
Fragments arranged by size:
1793 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 700
Size: 30 3794
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 799
Size: 3824
BseYI C'CCAG_C
Cuts at: 155 167 1249 2007 2584 3080 155
Size: 12 1082 758 577 496 899
Fragments arranged by size:
1082 899 758 577 496 12
BsiEI CG_ry'CG
Cuts at: 104 212 227 464 602 1366 1412 2620 104
Size: 108 15 237 138 764 46 1208 1308
Fragments arranged by size:
1308 1208 764 237 138 108 46 15
BsiHKAI G_wGCw'C
Cuts at: 542 838 1616 1806 2598 542
Size: 296 778 190 792 1768
Fragments arranged by size:
1768 792 778 296 190
Bsp1286I G_dGCh'C
Cuts at: 542 763 838 1449 1542 1616 1806 1868 2598
Size: 221 75 611 93 74 190 62 730
Cuts at: 2598 542
Size: 1768
Fragments arranged by size:
1768 730 611 221 190 93 75 74
62
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 874 1088 1390 1771 874
Size: 214 302 381 2927
Fragments arranged by size:
2927 381 302 214
BsrBI CCG'CTC
Cuts at: 50 134 774 2116 2170 3654 3819 50
Size: 84 640 1342 54 1484 165 55
Fragments arranged by size:
1484 1342 640 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 1736 1736
Size: 3824
BsrFI r'CCGG_y
Cuts at: 142 312 413 459 724 732 1822 2003 2212
Size: 170 101 46 265 8 1090 181 209
Cuts at: 2212 142
Size: 1754
Fragments arranged by size:
1754 1090 265 209 181 170 101 46
8
BssHII G'CGCG_C
Cuts at: 254 472 614 254
Size: 218 142 3464
Fragments arranged by size:
3464 218 142
BssSI C'ACGA_G
Cuts at: 2095 2453 2095
Size: 358 3466
BstAPI GCAn_nnn'nTGC
Cuts at: 627 1201 627
Size: 574 3250
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstYI r'GATC_y
Cuts at: 1 849 1316 1339 1674 1920 2932 3018 3030
Size: 848 467 23 335 246 1012 86 12
Cuts at: 3030 3376 1
Size: 346 449
Fragments arranged by size:
1012 848 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 333 333
Size: 3824
BtgI C'CryG_G
Cuts at: 107 206 218 467 719 107
Size: 99 12 249 252 3212
Fragments arranged by size:
3212 252 249 99 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 586 1067 586
Size: 481 3343
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2388 1530
Size: 858 2966
EaeI y'GGCC_r
Cuts at: 144 209 224 324 461 557 722 1409 1583
Size: 65 15 100 137 96 165 687 174
Cuts at: 1583 1974 2001 3346 144
Size: 391 27 1345 622
Fragments arranged by size:
1345 687 622 391 174 165 137 100
96 65 27 15
EagI C'GGCC_G
Cuts at: 209 224 461 1409 209
Size: 15 237 948 2624
Fragments arranged by size:
2624 948 237 15
EarI CTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2342 2488 3634 2032
Size: 310 146 1146 2222
Fragments arranged by size:
2222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 1649 2081 2828 3096 408
Size: 1241 432 747 268 1136
Fragments arranged by size:
1241 1136 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 963 963
Size: 3824
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 143 301 358 381 656 1258 1506 2528 3507
Size: 158 57 23 275 602 248 1022 979
Cuts at: 3507 143
Size: 460
Fragments arranged by size:
1022 979 602 460 275 248 158 57
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 745 777 1666 1696 1698 1728 700
Size: 32 13 32 889 30 2 30 2796
Fragments arranged by size:
2796 889 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 513 863 513
Size: 350 3474
KasI G'GCGC_C
Cuts at: 139 297 1254 1502 139
Size: 158 957 248 2461
Fragments arranged by size:
2461 957 248 158
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 728 731 2495 2679 728
Size: 3 1764 184 1873
Fragments arranged by size:
1873 1764 184 3
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 1813 1940 1813
Size: 127 3697
MspA1I CmG'CkG
Cuts at: 220 241 306 428 627 935 1249 1609 2622
Size: 21 65 122 199 308 314 360 1013
Cuts at: 2622 2867 220
Size: 245 1177
Fragments arranged by size:
1177 1013 360 314 308 245 199 122
65 21
NaeI GCC'GGC
Cuts at: 144 415 461 726 2005 144
Size: 271 46 265 1279 1963
Fragments arranged by size:
1963 1279 271 265 46
NarI GG'CG_CC
Cuts at: 140 298 1255 1503 140
Size: 158 957 248 2461
Fragments arranged by size:
2461 957 248 158
NcoI C'CATG_G
Cuts at: 107 719 107
Size: 612 3212
NgoMIV G'CCGG_C
Cuts at: 142 413 459 724 2003 142
Size: 271 46 265 1279 1963
Fragments arranged by size:
1963 1279 271 265 46
NotI GC'GGCC_GC
Cuts at: 224 224
Size: 3824
NspI r_CATG'y
Cuts at: 877 1377 2284 877
Size: 500 907 2417
Fragments arranged by size:
2417 907 500
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PstI C_TGCA'G
Cuts at: 623 871 1556 623
Size: 248 685 2891
Fragments arranged by size:
2891 685 248
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 306 627 1249 1609 306
Size: 321 622 360 2521
Fragments arranged by size:
2521 622 360 321
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SacII CC_GC'GG
Cuts at: 221 221
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 438 619 867 1552 2545 2736 33
Size: 405 181 248 685 993 191 1121
Fragments arranged by size:
1121 993 685 405 248 191 181
SfoI GGC'GCC
Cuts at: 141 299 1256 1504 141
Size: 158 957 248 2461
Fragments arranged by size:
2461 957 248 158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 846 846
Size: 3824
SmlI C'TyrA_G
Cuts at: 979 1894 2386 2648 979
Size: 915 492 262 2155
Fragments arranged by size:
2155 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StyI C'CwwG_G
Cuts at: 107 719 958 1312 107
Size: 612 239 354 2619
Fragments arranged by size:
2619 612 354 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 1540 1999 2208 1407
Size: 133 459 209 3023
Fragments arranged by size:
3023 459 209 133
TatI w'GTAC_w
Cuts at: 605 3339 605
Size: 2734 1090
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1319 1319
Size: 3824
XmaI C'CCGG_G
Cuts at: 844 844
Size: 3824
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI