Restriction map for
pF1KB9577
(Circular) (Six-base) MAPSORT of: pF1KB9577.seq Check: 8248 from: 1 to: 6746
pF1KB9577 6746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1843 2605 3312 1843
Size: 762 707 5277
Fragments arranged by size:
5277 762 707
AccI GT'mk_AC
Cuts at: 3784 3784
Size: 6746
Acc65I G'GTAC_C
Cuts at: 3762 3762
Size: 6746
AclI AA'CG_TT
Cuts at: 6586 6586
Size: 6746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 4571 5003 5356 6018 922
Size: 3649 432 353 662 1650
Fragments arranged by size:
3649 1650 662 432 353
AflII C'TTAA_G
Cuts at: 1782 1782
Size: 6746
AflIII A'CryG_T
Cuts at: 1685 2052 2753 2909 4098 5898 6179 1685
Size: 367 701 156 1189 1800 281 2252
Fragments arranged by size:
2252 1800 1189 701 367 281 156
AgeI A'CCGG_T
Cuts at: 3023 3023
Size: 6746
AleI CACnn'nnGTG
Cuts at: 628 2066 2752 3103 628
Size: 1438 686 351 4271
Fragments arranged by size:
4271 1438 686 351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 3010 3042 2042
Size: 32 936 32 5746
Fragments arranged by size:
5746 936 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1303 3237 3654 4051 5489 1303
Size: 1934 417 397 1438 2560
Fragments arranged by size:
2560 1934 1438 417 397
ApaLI G'TGCA_C
Cuts at: 5584 5584
Size: 6746
ApoI r'AATT_y
Cuts at: 1131 1963 2002 2294 3338 3750 6099 6355 1131
Size: 832 39 292 1044 412 2349 256 1522
Fragments arranged by size:
2349 1522 1044 832 412 292 256 39
AseI AT'TA_AT
Cuts at: 20 1151 1955 20
Size: 1131 804 4811
Fragments arranged by size:
4811 1131 804
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
AvaI C'yCGr_G
Cuts at: 190 458 3079 3766 190
Size: 268 2621 687 3170
Fragments arranged by size:
3170 2621 687 268
AvrII C'CTAG_G
Cuts at: 163 163
Size: 6746
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2929 2962 3391 3424 2929
Size: 33 429 33 6251
Fragments arranged by size:
6251 429 33 33
BamHI G'GATC_C
Cuts at: 593 3771 593
Size: 3178 3568
BanI G'GyrC_C
Cuts at: 2256 3699 3762 4176 4424 4459 2256
Size: 1443 63 414 248 35 4543
Fragments arranged by size:
4543 1443 414 248 63 35
BanII G_rGCy'C
Cuts at: 191 197 560 1391 3737 3760 4790 191
Size: 6 363 831 2346 23 1030 2147
Fragments arranged by size:
2346 2147 1030 831 363 23 6
BbeI G_GCGC'C
Cuts at: 4180 4428 4180
Size: 248 6498
BbsI GAAGACnn'nnnn_
Cuts at: 422 2660 422
Size: 2238 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3070 3104 90
Size: 34 2946 34 3732
Fragments arranged by size:
3732 2946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4644 5700 6746 4644
Size: 1056 1046 4644
Fragments arranged by size:
4644 1056 1046
BclI T'GATC_A
Cuts at: 4266 4266
Size: 6746
BglI GCCn_nnn'nGGC
Cuts at: 1314 3057 1314
Size: 1743 5003
BglII A'GATC_T
Cuts at: 4261 6298 4261
Size: 2037 4709
BlpI GC'TnA_GC
Cuts at: 112 2204 3858 112
Size: 2092 1654 3000
Fragments arranged by size:
3000 2092 1654
Bme1580I G_kGCm'C
Cuts at: 1096 2744 4371 4464 5588 1096
Size: 1648 1627 93 1124 2254
Fragments arranged by size:
2254 1648 1627 1124 93
BmgBI CAC'GTC
Cuts at: 771 2055 771
Size: 1284 5462
BmrI ACTGGGnnnn_n'
Cuts at: 582 2329 2863 3688 3958 4039 4374 6518 582
Size: 1747 534 825 270 81 335 2144 810
Fragments arranged by size:
2144 1747 825 810 534 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1859 5113 1859
Size: 3254 3492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 205 273 553 837 1214 1310 1831 3255
Size: 40 68 280 284 377 96 521 1424
Cuts at: 3255 165
Size: 3656
Fragments arranged by size:
3656 1424 521 377 284 280 96 68
40
Bpu10I CC'TnA_GC
Cuts at: 1304 2706 1304
Size: 1402 5344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1551 1747 1862 3922 4801 5515 5813 1551
Size: 196 115 2060 879 714 298 2484
Fragments arranged by size:
2484 2060 879 714 298 196 115
BsaI GGTCTCn'nnnn_
Cuts at: 2843 3563 6450 2843
Size: 720 2887 3139
Fragments arranged by size:
3139 2887 720
BsaAI yAC'GTr
Cuts at: 608 1917 2754 4099 608
Size: 1309 837 1345 3255
Fragments arranged by size:
3255 1345 1309 837
BsaBI GATnn'nnATC
Cuts at: 6 2686 4260 6297 6
Size: 2680 1574 2037 455
Fragments arranged by size:
2680 2037 1574 455
BsaHI Gr'CG_yC
Cuts at: 854 2624 3299 4177 4425 5127 6623 854
Size: 1770 675 878 248 702 1496 977
Fragments arranged by size:
1770 1496 977 878 702 675 248
BsaWI w'CCGG_w
Cuts at: 590 2678 3023 3314 3935 4456 5134 5545 5692
Size: 2088 345 291 621 521 678 411 147
Cuts at: 5692 5966 590
Size: 274 1370
Fragments arranged by size:
2088 1370 678 621 521 411 345 291
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 560 571 590 601 2720 2750 3253
Size: 30 4 11 19 11 2119 30 503
Cuts at: 3253 3283 526
Size: 30 3989
Fragments arranged by size:
3989 2119 503 30 30 30 19 11
11 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1471 1744 2233 2623 3403 1471
Size: 273 489 390 780 4814
Fragments arranged by size:
4814 780 489 390 273
BseYI C'CCAG_C
Cuts at: 1040 2770 3117 3122 3543 3736 4171 4929 5594
Size: 1730 347 5 421 193 435 758 665
Cuts at: 5594 6002 1040
Size: 408 1784
Fragments arranged by size:
1784 1730 758 665 435 421 408 347
193 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2530 3626 2530
Size: 1096 5650
BsiEI CG_ry'CG
Cuts at: 104 3221 3431 4288 4334 5564 104
Size: 3117 210 857 46 1230 1286
Fragments arranged by size:
3117 1286 1230 857 210 46
BsiHKAI G_wGCw'C
Cuts at: 197 1391 3760 4538 4728 5588 197
Size: 1194 2369 778 190 860 1355
Fragments arranged by size:
2369 1355 1194 860 778 190
BsiWI C'GTAC_G
Cuts at: 3432 3432
Size: 6746
BsmI GAATG_Cn'
Cuts at: 2797 2797
Size: 6746
BsmBI CGTCTCn'nnnn_
Cuts at: 845 2639 845
Size: 1794 4952
Bsp1286I G_dGCh'C
Cuts at: 191 197 560 1096 1391 2744 3737 3760 4371
Size: 6 363 536 295 1353 993 23 611
Cuts at: 4371 4464 4538 4728 4790 5588 191
Size: 93 74 190 62 798 1349
Fragments arranged by size:
1353 1349 993 798 611 536 363 295
190 93 74 62 23 6
BspEI T'CCGG_A
Cuts at: 2678 2678
Size: 6746
BspHI T'CATG_A
Cuts at: 867 1929 5178 867
Size: 1062 3249 2435
Fragments arranged by size:
3249 2435 1062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1843 2252 2591 2605 3312 3796 4010 4312 4693
Size: 409 339 14 707 484 214 302 381
Cuts at: 4693 1843
Size: 3896
Fragments arranged by size:
3896 707 484 409 381 339 302 214
14
BsrBI CCG'CTC
Cuts at: 50 1772 5038 5092 6576 6741 50
Size: 1722 3266 54 1484 165 55
Fragments arranged by size:
3266 1722 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4658 4658
Size: 6746
BsrFI r'CCGG_y
Cuts at: 2247 2568 3023 4744 4925 2247
Size: 321 455 1721 181 4068
Fragments arranged by size:
4068 1721 455 321 181
BssHII G'CGCG_C
Cuts at: 1474 1474
Size: 6746
BssSI C'ACGA_G
Cuts at: 2530 2548 3031 5017 5725 2530
Size: 18 483 1986 708 3551
Fragments arranged by size:
3551 1986 708 483 18
BstAPI GCAn_nnn'nTGC
Cuts at: 3654 4123 3654
Size: 469 6277
BstBI TT'CG_AA
Cuts at: 5107 5107
Size: 6746
BstEII G'GTnAC_C
Cuts at: 3105 3105
Size: 6746
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1490 132
Size: 1358 5388
BstYI r'GATC_y
Cuts at: 1 593 1797 3771 4238 4261 4596 4842 5148
Size: 592 1204 1974 467 23 335 246 306
Cuts at: 5148 5160 5246 6298 1
Size: 12 86 1052 449
Fragments arranged by size:
1974 1204 1052 592 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 432 974 1097 2576 2891 3162 432
Size: 542 123 1479 315 271 4016
Fragments arranged by size:
4016 1479 542 315 271 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4696 4877 5040 4696
Size: 181 163 6402
Fragments arranged by size:
6402 181 163
BtsI GCAGTG_nn'
Cuts at: 788 2160 3643 3989 788
Size: 1372 1483 346 3545
Fragments arranged by size:
3545 1483 1372 346
ClaI AT'CG_AT
Cuts at: 3465 3465
Size: 6746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2136 2171 2136
Size: 35 6711
DraI TTT'AAA
Cuts at: 1871 3745 1871
Size: 1874 4872
DrdI GACnn_nn'nnGTC
Cuts at: 3298 3435 4452 5796 3298
Size: 137 1017 1344 4248
Fragments arranged by size:
4248 1344 1017 137
EaeI y'GGCC_r
Cuts at: 1374 1826 2570 2829 3218 4331 4505 4896 4923
Size: 452 744 259 389 1113 174 391 27
Cuts at: 4923 6268 1374
Size: 1345 1852
Fragments arranged by size:
1852 1345 1113 744 452 391 389 259
174 27
EagI C'GGCC_G
Cuts at: 3218 4331 3218
Size: 1113 5633
EarI CTCTTCn'nnn_
Cuts at: 838 4769 4979 838
Size: 3931 210 2605
Fragments arranged by size:
3931 2605 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 169 4954 5696 5842 6556 169
Size: 4785 742 146 714 359
Fragments arranged by size:
4785 742 714 359 146
EcoICRI GAG'CTC
Cuts at: 195 1389 3758 195
Size: 1194 2369 3183
Fragments arranged by size:
3183 2369 1194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 205 273 553 837 922 1214 1310 1831
Size: 40 68 280 284 85 292 96 521
Cuts at: 1831 3255 4571 5003 5356 6018 165
Size: 1424 1316 432 353 662 893
Fragments arranged by size:
1424 1316 893 662 521 432 353 292
284 280 96 85 68 40
EcoNI CCTnn'n_nnAGG
Cuts at: 490 3140 490
Size: 2650 4096
EcoO109I rG'GnC_Cy
Cuts at: 1815 2241 3036 3234 3885 1815
Size: 426 795 198 651 4676
Fragments arranged by size:
4676 795 651 426 198
EcoRI G'AATT_C
Cuts at: 1131 3338 3750 1131
Size: 2207 412 4127
Fragments arranged by size:
4127 2207 412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3162 4765 3162
Size: 1603 5143
FspI TGC'GCA
Cuts at: 4527 4527
Size: 6746
HaeII r_GCGC'y
Cuts at: 826 4180 4428 5658 6429 826
Size: 3354 248 1230 771 1143
Fragments arranged by size:
3354 1230 1143 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1120 1152 1883 1915 2642 2674 4588 4618 4620
Size: 32 731 32 727 32 1914 30 2
Cuts at: 4620 4650 1120
Size: 30 3216
Fragments arranged by size:
3216 1914 731 727 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1520 3785 1520
Size: 2265 4481
HindIII A'AGCT_T
Cuts at: 1498 3527 1498
Size: 2029 4717
HpaI GTT'AAC
Cuts at: 1520 1520
Size: 6746
KasI G'GCGC_C
Cuts at: 4176 4424 4176
Size: 248 6498
KpnI G_GTAC'C
Cuts at: 3766 3766
Size: 6746
MluI A'CGCG_T
Cuts at: 6179 6179
Size: 6746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 306 1175 2470 2537 2798 3357 3512 5505 5689
Size: 869 1295 67 261 559 155 1993 184
Cuts at: 5689 306
Size: 1363
Fragments arranged by size:
1993 1363 1295 869 559 261 184 155
67
MscI TGG'CCA
Cuts at: 2831 4507 2831
Size: 1676 5070
MslI CAynn'nnrTG
Cuts at: 628 884 2066 2752 3103 4735 4862 628
Size: 256 1182 686 351 1632 127 2512
Fragments arranged by size:
2512 1632 1182 686 351 256 127
MspA1I CmG'CkG
Cuts at: 183 1025 1379 3857 4171 4531 5315 5560 183
Size: 842 354 2478 314 360 784 245 1369
Fragments arranged by size:
2478 1369 842 784 360 354 314 245
NaeI GCC'GGC
Cuts at: 2570 4927 2570
Size: 2357 4389
NarI GG'CG_CC
Cuts at: 4177 4425 4177
Size: 248 6498
NcoI C'CATG_G
Cuts at: 432 974 2576 2891 3162 432
Size: 542 1602 315 271 4016
Fragments arranged by size:
4016 1602 542 315 271
NgoMIV G'CCGG_C
Cuts at: 2568 4925 2568
Size: 2357 4389
NspI r_CATG'y
Cuts at: 1689 1897 2699 3799 4299 5902 1689
Size: 208 802 1100 500 1603 2533
Fragments arranged by size:
2533 1603 1100 802 500 208
PasI CC'CwG_GG
Cuts at: 1819 1819
Size: 6746
PciI A'CATG_T
Cuts at: 1685 5898 1685
Size: 4213 2533
PflMI CCAn_nnn'nTGG
Cuts at: 1074 1074
Size: 6746
PmeI GTTT'AAAC
Cuts at: 3745 3745
Size: 6746
PmlI CAC'GTG
Cuts at: 608 2754 608
Size: 2146 4600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 5192
Size: 32 6714
PpuMI rG'GwC_Cy
Cuts at: 1815 2241 3234 1815
Size: 426 993 5327
Fragments arranged by size:
5327 993 426
PshAI GACnn'nnGTC
Cuts at: 2390 2390
Size: 6746
PspXI vC'TCGA_Gb
Cuts at: 3079 3079
Size: 6746
PstI C_TGCA'G
Cuts at: 253 2271 2602 3650 3793 4478 253
Size: 2018 331 1048 143 685 2521
Fragments arranged by size:
2521 2018 1048 685 331 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6746
PvuII CAG'CTG
Cuts at: 183 1025 4171 4531 183
Size: 842 3146 360 2398
Fragments arranged by size:
3146 2398 842 360
RsrII CG'GwC_CG
Cuts at: 4941 4941
Size: 6746
SacI G_AGCT'C
Cuts at: 197 1391 3760 197
Size: 1194 2369 3183
Fragments arranged by size:
3183 2369 1194
SalI G'TCGA_C
Cuts at: 3783 3783
Size: 6746
SapI GCTCTTCn'nnn_
Cuts at: 4769 4979 4769
Size: 210 6536
SbfI CC_TGCA'GG
Cuts at: 2602 3793 2602
Size: 1191 5555
ScaI AGT'ACT
Cuts at: 6263 6263
Size: 6746
SfcI C'TryA_G
Cuts at: 33 249 1535 2267 2563 2598 3646 3789 4474
Size: 216 1286 732 296 35 1048 143 685
Cuts at: 4474 5442 5633 33
Size: 968 191 1146
Fragments arranged by size:
1286 1146 1048 968 732 685 296 216
191 143 35
SfoI GGC'GCC
Cuts at: 4178 4426 4178
Size: 248 6498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
SmaI CCC'GGG
Cuts at: 3768 3768
Size: 6746
SmlI C'TyrA_G
Cuts at: 1566 1726 1782 1877 3079 3901 4816 5530 5792
Size: 160 56 95 1202 822 915 714 262
Cuts at: 5792 1566
Size: 2520
Fragments arranged by size:
2520 1202 915 822 714 262 160 95
56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6746
SphI G_CATG'C
Cuts at: 3799 4299 3799
Size: 500 6246
SspI AAT'ATT
Cuts at: 1149 1149
Size: 6746
StuI AGG'CCT
Cuts at: 1839 1839
Size: 6746
StyI C'CwwG_G
Cuts at: 163 432 722 974 2576 2891 3162 3880 4234
Size: 269 290 252 1602 315 271 718 354
Cuts at: 4234 163
Size: 2675
Fragments arranged by size:
2675 1602 718 354 315 290 271 269
252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 564 4329 4462 4921 5130 564
Size: 3765 133 459 209 2180
Fragments arranged by size:
3765 2180 459 209 133
TatI w'GTAC_w
Cuts at: 1883 6261 1883
Size: 4378 2368
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 320 352 526 558 560 592 2364 2396 2423
Size: 32 174 32 2 32 1772 32 27
Cuts at: 2423 2455 3253 3285 6263 6295 320
Size: 32 798 32 2978 32 771
Fragments arranged by size:
2978 1772 798 771 174 32 32 32
32 32 32 32 27 2
Tth111I GACn'n_nGTC
Cuts at: 661 772 2033 2626 4543 661
Size: 111 1261 593 1917 2864
Fragments arranged by size:
2864 1917 1261 593 111
XbaI T'CTAG_A
Cuts at: 3777 3777
Size: 6746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2889 4241 2206
Size: 683 1352 4711
Fragments arranged by size:
4711 1352 683
XhoI C'TCGA_G
Cuts at: 3079 3079
Size: 6746
XmaI C'CCGG_G
Cuts at: 3766 3766
Size: 6746
XmnI GAAnn'nnTTC
Cuts at: 3145 3145
Size: 6746
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI ApaI AscI BbvCI BfrBI
BmtI BsrGI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI ZraI