Restriction map for
pF1KB9575
(Circular) (Six-base) MAPSORT of: pF1KB9575.seq Check: 4491 from: 1 to: 4934
pF1KB9575 4934 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 941 974 1972 941
Size: 33 998 3903
Fragments arranged by size:
3903 998 33
Acc65I G'GTAC_C
Cuts at: 1950 1950
Size: 4934
AclI AA'CG_TT
Cuts at: 4774 4774
Size: 4934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1035 1588 1648 1927 2759 3191 3938 4206
Size: 433 553 60 279 832 432 747 268
Cuts at: 4206 602
Size: 1330
Fragments arranged by size:
1330 832 747 553 433 432 279 268
60
AflIII A'CryG_T
Cuts at: 2286 3390 4367 2286
Size: 1104 977 2853
Fragments arranged by size:
2853 1104 977
AgeI A'CCGG_T
Cuts at: 3322 3322
Size: 4934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 353 674 1355 353
Size: 321 681 3932
Fragments arranged by size:
3932 681 321
AlwNI CAG_nnn'CTG
Cuts at: 1566 2239 3806 1566
Size: 673 1567 2694
Fragments arranged by size:
2694 1567 673
ApaLI G'TGCA_C
Cuts at: 1528 3704 1528
Size: 2176 2758
ApoI r'AATT_y
Cuts at: 1771 1882 1938 4287 4543 1771
Size: 111 56 2349 256 2162
Fragments arranged by size:
2349 2162 256 111 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 4934
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
AvaI C'yCGr_G
Cuts at: 150 574 1954 150
Size: 424 1380 3130
Fragments arranged by size:
3130 1380 424
BamHI G'GATC_C
Cuts at: 1697 1959 1697
Size: 262 4672
BanI G'GyrC_C
Cuts at: 1950 2364 2612 2647 1950
Size: 414 248 35 4237
Fragments arranged by size:
4237 414 248 35
BanII G_rGCy'C
Cuts at: 1553 1948 2978 1553
Size: 395 1030 3509
Fragments arranged by size:
3509 1030 395
BbeI G_GCGC'C
Cuts at: 2368 2616 2368
Size: 248 4686
BbsI GAAGACnn'nnnn_
Cuts at: 609 1233 1704 609
Size: 624 471 3839
Fragments arranged by size:
3839 624 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 770 736
Size: 34 4900
BciVI GTATCCnnnnn_n'
Cuts at: 2832 3593 4934 2832
Size: 761 1341 2832
Fragments arranged by size:
2832 1341 761
BclI T'GATC_A
Cuts at: 389 2454 389
Size: 2065 2869
BfrBI ATG'CAT
Cuts at: 1338 1338
Size: 4934
BglI GCCn_nnn'nGGC
Cuts at: 1660 1660
Size: 4934
BglII A'GATC_T
Cuts at: 2449 4486 2449
Size: 2037 2897
BlpI GC'TnA_GC
Cuts at: 2046 2046
Size: 4934
Bme1580I G_kGCm'C
Cuts at: 1514 1532 2559 2652 3708 1514
Size: 18 1027 93 1056 2740
Fragments arranged by size:
2740 1056 1027 93 18
BmrI ACTGGGnnnn_n'
Cuts at: 2146 2227 2562 4706 2146
Size: 81 335 2144 2374
Fragments arranged by size:
2374 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 4934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1138 1384 459
Size: 679 246 4009
Fragments arranged by size:
4009 679 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2110 2989 3481 3779 2110
Size: 879 492 298 3265
Fragments arranged by size:
3265 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1917 4638 1917
Size: 2721 2213
BsaAI yAC'GTr
Cuts at: 1298 2287 1298
Size: 989 3945
BsaBI GATnn'nnATC
Cuts at: 6 1822 2448 4485 6
Size: 1816 626 2037 455
Fragments arranged by size:
2037 1816 626 455
BsaHI Gr'CG_yC
Cuts at: 2365 2613 3315 4811 2365
Size: 248 702 1496 2488
Fragments arranged by size:
2488 1496 702 248
BsaWI w'CCGG_w
Cuts at: 133 1694 2123 2644 3322 3596 3743 4154 133
Size: 1561 429 521 678 274 147 411 913
Fragments arranged by size:
1561 913 678 521 429 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 679
Size: 30 4904
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 880 1246 1903 877
Size: 3 366 657 3908
Fragments arranged by size:
3908 657 366 3
BseYI C'CCAG_C
Cuts at: 295 1006 1601 2359 3117 3694 4190 295
Size: 711 595 758 758 577 496 1039
Fragments arranged by size:
1039 758 758 711 595 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1060
Size: 4934
BsiEI CG_ry'CG
Cuts at: 104 2476 2522 3730 104
Size: 2372 46 1208 1308
Fragments arranged by size:
2372 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 1532 1948 2726 2916 3708 221
Size: 1311 416 778 190 792 1447
Fragments arranged by size:
1447 1311 792 778 416 190
BsmI GAATG_Cn'
Cuts at: 1391 1391
Size: 4934
Bsp1286I G_dGCh'C
Cuts at: 221 1514 1532 1553 1948 2559 2652 2726 2916
Size: 1293 18 21 395 611 93 74 190
Cuts at: 2916 2978 3708 221
Size: 62 730 1447
Fragments arranged by size:
1447 1293 730 611 395 190 93 74
62 21 18
BspEI T'CCGG_A
Cuts at: 1694 4154 1694
Size: 2460 2474
BspHI T'CATG_A
Cuts at: 4110 4110
Size: 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1984 2198 2500 2881 1984
Size: 214 302 381 4037
Fragments arranged by size:
4037 381 302 214
BsrBI CCG'CTC
Cuts at: 50 195 3226 3280 4764 4929 50
Size: 145 3031 54 1484 165 55
Fragments arranged by size:
3031 1484 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 587 612 1352 2846 587
Size: 25 740 1494 2675
Fragments arranged by size:
2675 1494 740 25
BsrFI r'CCGG_y
Cuts at: 2932 3113 3322 2932
Size: 181 209 4544
Fragments arranged by size:
4544 209 181
BssSI C'ACGA_G
Cuts at: 1735 3205 3563 1735
Size: 1470 358 3106
Fragments arranged by size:
3106 1470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1344 2311 1344
Size: 967 3967
BstBI TT'CG_AA
Cuts at: 3295 3295
Size: 4934
BstEII G'GTnAC_C
Cuts at: 988 1173 1447 988
Size: 185 274 4475
Fragments arranged by size:
4475 274 185
BstXI CCAn_nnnn'nTGG
Cuts at: 1650 1650
Size: 4934
BstYI r'GATC_y
Cuts at: 1 321 506 1697 1874 1959 2426 2449 2784
Size: 320 185 1191 177 85 467 23 335
Cuts at: 2784 3030 4042 4128 4140 4486 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1191 1012 467 449 346 335 320 246
185 177 86 85 23 12
BstZ17I GTA'TAC
Cuts at: 975 975
Size: 4934
Bsu36I CC'TnA_GG
Cuts at: 174 383 174
Size: 209 4725
BtgI C'CryG_G
Cuts at: 107 107
Size: 4934
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1809 2884 3065 3228 1809
Size: 1075 181 163 3515
Fragments arranged by size:
3515 1075 181 163
BtsI GCAGTG_nn'
Cuts at: 2177 2177
Size: 4934
ClaI AT'CG_AT
Cuts at: 797 797
Size: 4934
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 651 686 651
Size: 35 4899
DraI TTT'AAA
Cuts at: 1933 1933
Size: 4934
DrdI GACnn_nn'nnGTC
Cuts at: 1456 1837 2640 3498 1456
Size: 381 803 858 2892
Fragments arranged by size:
2892 858 803 381
EaeI y'GGCC_r
Cuts at: 110 1349 1652 2519 2693 3084 3111 4456 110
Size: 1239 303 867 174 391 27 1345 588
Fragments arranged by size:
1345 1239 867 588 391 303 174 27
EagI C'GGCC_G
Cuts at: 2519 2519
Size: 4934
EarI CTCTTCn'nnn_
Cuts at: 730 1135 2957 3167 730
Size: 405 1822 210 2497
Fragments arranged by size:
2497 1822 405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1060 3142 3452 3598 4744 1060
Size: 2082 310 146 1146 1250
Fragments arranged by size:
2082 1250 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1946 1946
Size: 4934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 602 1035 1138 1384 1588 1648 1927 2759
Size: 143 433 103 246 204 60 279 832
Cuts at: 2759 3191 3938 4206 459
Size: 432 747 268 1187
Fragments arranged by size:
1187 832 747 433 432 279 268 246
204 143 103 60
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 4934
EcoO109I rG'GnC_Cy
Cuts at: 333 676 2073 333
Size: 343 1397 3194
Fragments arranged by size:
3194 1397 343
EcoRI G'AATT_C
Cuts at: 1938 1938
Size: 4934
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4934
FspI TGC'GCA
Cuts at: 1435 2715 1435
Size: 1280 3654
HaeII r_GCGC'y
Cuts at: 2368 2616 3638 4617 2368
Size: 248 1022 979 2685
Fragments arranged by size:
2685 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 1200 1232 2776 2806 2808 2838 247
Size: 32 921 32 1544 30 2 30 2343
Fragments arranged by size:
2343 1544 921 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1794 1973 1794
Size: 179 4755
KasI G'GCGC_C
Cuts at: 2364 2612 2364
Size: 248 4686
KpnI G_GTAC'C
Cuts at: 1954 1954
Size: 4934
MluI A'CGCG_T
Cuts at: 4367 4367
Size: 4934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 947 974 3605 3789 947
Size: 27 2631 184 2092
Fragments arranged by size:
2631 2092 184 27
MscI TGG'CCA
Cuts at: 112 1351 1654 2695 112
Size: 1239 303 1041 2351
Fragments arranged by size:
2351 1239 1041 303
MslI CAynn'nnrTG
Cuts at: 656 1111 1642 1648 2923 3050 656
Size: 455 531 6 1275 127 2540
Fragments arranged by size:
2540 1275 531 455 127 6
MspA1I CmG'CkG
Cuts at: 250 530 839 1404 1566 1748 2045 2359 2719
Size: 280 309 565 162 182 297 314 360
Cuts at: 2719 3732 3977 250
Size: 1013 245 1207
Fragments arranged by size:
1207 1013 565 360 314 309 297 280
245 182 162
NaeI GCC'GGC
Cuts at: 3115 3115
Size: 4934
NarI GG'CG_CC
Cuts at: 2365 2613 2365
Size: 248 4686
NcoI C'CATG_G
Cuts at: 107 107
Size: 4934
NgoMIV G'CCGG_C
Cuts at: 3113 3113
Size: 4934
NsiI A_TGCA'T
Cuts at: 1340 1340
Size: 4934
NspI r_CATG'y
Cuts at: 1284 1342 1440 1987 2487 3394 1284
Size: 58 98 547 500 907 2824
Fragments arranged by size:
2824 907 547 500 98 58
PciI A'CATG_T
Cuts at: 3390 3390
Size: 4934
PflMI CCAn_nnn'nTGG
Cuts at: 601 779 899 601
Size: 178 120 4636
Fragments arranged by size:
4636 178 120
PfoI T'CCnGG_A
Cuts at: 509 1670 509
Size: 1161 3773
PmeI GTTT'AAAC
Cuts at: 1933 1933
Size: 4934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1675 1707 4073 4105 1675
Size: 32 2366 32 2504
Fragments arranged by size:
2504 2366 32 32
PpuMI rG'GwC_Cy
Cuts at: 333 676 333
Size: 343 4591
PstI C_TGCA'G
Cuts at: 1562 1981 2666 1562
Size: 419 685 3830
Fragments arranged by size:
3830 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4934
PvuII CAG'CTG
Cuts at: 530 1404 1566 2359 2719 530
Size: 874 162 793 360 2745
Fragments arranged by size:
2745 874 793 360 162
RsrII CG'GwC_CG
Cuts at: 3129 3129
Size: 4934
SacI G_AGCT'C
Cuts at: 1948 1948
Size: 4934
SalI G'TCGA_C
Cuts at: 1971 1971
Size: 4934
SapI GCTCTTCn'nnn_
Cuts at: 2957 3167 2957
Size: 210 4724
SbfI CC_TGCA'GG
Cuts at: 1981 1981
Size: 4934
ScaI AGT'ACT
Cuts at: 4451 4451
Size: 4934
SfcI C'TryA_G
Cuts at: 33 457 645 1505 1558 1977 2662 3655 3846
Size: 424 188 860 53 419 685 993 191
Cuts at: 3846 33
Size: 1121
Fragments arranged by size:
1121 993 860 685 424 419 191 188
53
SfoI GGC'GCC
Cuts at: 2366 2614 2366
Size: 248 4686
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
SmaI CCC'GGG
Cuts at: 1956 1956
Size: 4934
SmlI C'TyrA_G
Cuts at: 2089 3004 3496 3758 2089
Size: 915 492 262 3265
Fragments arranged by size:
3265 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4934
SphI G_CATG'C
Cuts at: 1342 1440 1987 2487 1342
Size: 98 547 500 3789
Fragments arranged by size:
3789 547 500 98
StuI AGG'CCT
Cuts at: 119 119
Size: 4934
StyI C'CwwG_G
Cuts at: 107 568 683 1835 2068 2422 107
Size: 461 115 1152 233 354 2619
Fragments arranged by size:
2619 1152 461 354 233 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2517 2650 3109 3318 2517
Size: 133 459 209 4133
Fragments arranged by size:
4133 459 209 133
TatI w'GTAC_w
Cuts at: 1587 4449 1587
Size: 2862 2072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 306 338 4451 4483 306
Size: 32 4113 32 757
Fragments arranged by size:
4113 757 32 32
Tth111I GACn'n_nGTC
Cuts at: 2731 2731
Size: 4934
XbaI T'CTAG_A
Cuts at: 1965 1965
Size: 4934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1113 1647 2429 1113
Size: 534 782 3618
Fragments arranged by size:
3618 782 534
XmaI C'CCGG_G
Cuts at: 1954 1954
Size: 4934
XmnI GAAnn'nnTTC
Cuts at: 129 129
Size: 4934
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI