Restriction map for
pF1KB9573
(Circular) (Six-base) MAPSORT of: pF1KB9573.seq Check: 1099 from: 1 to: 4238
pF1KB9573 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 2063 2495 2848 3510 821
Size: 1242 432 353 662 1549
Fragments arranged by size:
1549 1242 662 432 353
AflIII A'CryG_T
Cuts at: 1590 3390 3671 1590
Size: 1800 281 2157
Fragments arranged by size:
2157 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 4238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 756 788 756
Size: 32 4206
AlwNI CAG_nnn'CTG
Cuts at: 463 1543 2981 463
Size: 1080 1438 1720
Fragments arranged by size:
1720 1438 1080
ApaLI G'TGCA_C
Cuts at: 3076 3076
Size: 4238
ApoI r'AATT_y
Cuts at: 559 648 779 1242 3591 3847 559
Size: 89 131 463 2349 256 950
Fragments arranged by size:
2349 950 463 256 131 89
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 1252 2282 1252
Size: 1030 3208
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 827 827
Size: 4238
BciVI GTATCCnnnnn_n'
Cuts at: 380 2136 3192 4238 380
Size: 1756 1056 1046 380
Fragments arranged by size:
1756 1056 1046 380
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BfrBI ATG'CAT
Cuts at: 660 660
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 758 1350 758
Size: 592 3646
Bme1580I G_kGCm'C
Cuts at: 528 1863 1956 3080 528
Size: 1335 93 1124 1686
Fragments arranged by size:
1686 1335 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1450 1531 1866 4010 1450
Size: 81 335 2144 1678
Fragments arranged by size:
2144 1678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 3007 3305 1414
Size: 879 714 298 2347
Fragments arranged by size:
2347 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 4238
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 3037 3184 3458 1427
Size: 521 678 411 147 274 2207
Fragments arranged by size:
2207 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 220 244 247 250 253 256 880 211
Size: 9 24 3 3 3 3 624 3569
Fragments arranged by size:
3569 624 24 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 152 1663 2421 3086 3494 152
Size: 1511 758 665 408 896
Fragments arranged by size:
1511 896 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3056 104
Size: 1676 46 1230 1286
Fragments arranged by size:
1676 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1252 2030 2220 3080 1252
Size: 778 190 860 2410
Fragments arranged by size:
2410 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 528 1252 1863 1956 2030 2220 2282 3080 528
Size: 724 611 93 74 190 62 798 1686
Fragments arranged by size:
1686 798 724 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2670 2670
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1502 1804 2185 1288
Size: 214 302 381 3341
Fragments arranged by size:
3341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 682 2150 682
Size: 1468 2770
BsrFI r'CCGG_y
Cuts at: 2236 2417 2236
Size: 181 4057
BssSI C'ACGA_G
Cuts at: 2509 3217 2509
Size: 708 3530
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 641 1263 1730 1753 2088 2334 2640 2652
Size: 640 622 467 23 335 246 306 12
Cuts at: 2652 2738 3790 1
Size: 86 1052 449
Fragments arranged by size:
1052 640 622 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 3288 1944
Size: 1344 2894
EaeI y'GGCC_r
Cuts at: 1106 1823 1997 2388 2415 3760 1106
Size: 717 174 391 27 1345 1584
Fragments arranged by size:
1584 1345 717 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 206 324 631 824 857 1070 2261 2471 206
Size: 118 307 193 33 213 1191 210 1973
Fragments arranged by size:
1973 1191 307 213 210 193 118 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 102 2446 3188 3334 4048 102
Size: 2344 742 146 714 292
Fragments arranged by size:
2344 742 714 292 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 2063 2495 2848 3510 821
Size: 1242 432 353 662 1549
Fragments arranged by size:
1549 1242 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 660 2257 660
Size: 1597 2641
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 3150 3921 1672
Size: 248 1230 771 1989
Fragments arranged by size:
1989 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2080 2110 2112 2142 2080
Size: 30 2 30 4176
Fragments arranged by size:
4176 30 30 2
HincII GTy'rAC
Cuts at: 305 1277 305
Size: 972 3266
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MfeI C'AATT_G
Cuts at: 344 344
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2997 3181 2997
Size: 184 4054
MscI TGG'CCA
Cuts at: 1108 1999 1108
Size: 891 3347
MslI CAynn'nnrTG
Cuts at: 2227 2354 2227
Size: 127 4111
MspA1I CmG'CkG
Cuts at: 1349 1663 2023 2807 3052 1349
Size: 314 360 784 245 2535
Fragments arranged by size:
2535 784 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NsiI A_TGCA'T
Cuts at: 662 662
Size: 4238
NspI r_CATG'y
Cuts at: 1291 1791 3394 1291
Size: 500 1603 2135
Fragments arranged by size:
2135 1603 500
PacI TTA_AT'TAA
Cuts at: 1046 1046
Size: 4238
PciI A'CATG_T
Cuts at: 3390 3390
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 4206
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 1067 1281 1966 2934 3125 33
Size: 1034 214 685 968 191 1146
Fragments arranged by size:
1146 1034 968 685 214 191
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1393 2308 3022 3284 1393
Size: 915 714 262 2347
Fragments arranged by size:
2347 915 714 262
SpeI A'CTAG_T
Cuts at: 67 630 67
Size: 563 3675
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 1372 1726 1372
Size: 354 3884
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 1821 1954 2413 2622 166
Size: 1655 133 459 209 1782
Fragments arranged by size:
1782 1655 459 209 133
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1116 1733 1116
Size: 617 3621
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PacI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI