Restriction map for
pF1KB9571
(Circular) (Six-base) MAPSORT of: pF1KB9571.seq Check: 9363 from: 1 to: 4067
pF1KB9571 4067 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 372 372
Size: 4067
AccI GT'mk_AC
Cuts at: 118 1105 118
Size: 987 3080
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1892 2324 2677 3339 530
Size: 1362 432 353 662 1258
Fragments arranged by size:
1362 1258 662 432 353
AflII C'TTAA_G
Cuts at: 847 847
Size: 4067
AflIII A'CryG_T
Cuts at: 1419 3219 3500 1419
Size: 1800 281 1986
Fragments arranged by size:
1986 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 491 953 491
Size: 462 3605
AlwNI CAG_nnn'CTG
Cuts at: 577 1372 2810 577
Size: 795 1438 1834
Fragments arranged by size:
1834 1438 795
ApaLI G'TGCA_C
Cuts at: 2905 2905
Size: 4067
ApoI r'AATT_y
Cuts at: 890 1071 3420 3676 890
Size: 181 2349 256 1281
Fragments arranged by size:
2349 1281 256 181
AseI AT'TA_AT
Cuts at: 20 199 20
Size: 179 3888
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 863 1087 863
Size: 224 3843
BamHI G'GATC_C
Cuts at: 1011 1092 1011
Size: 81 3986
BanI G'GyrC_C
Cuts at: 550 929 1083 1497 1745 1780 550
Size: 379 154 414 248 35 2837
Fragments arranged by size:
2837 414 379 248 154 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 941 907
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 3021 4067 1965
Size: 1056 1046 1965
Fragments arranged by size:
1965 1056 1046
BclI T'GATC_A
Cuts at: 590 1587 590
Size: 997 3070
BglI GCCn_nnn'nGGC
Cuts at: 398 613 398
Size: 215 3852
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2909 1692
Size: 93 1124 2850
Fragments arranged by size:
2850 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 806 544
Size: 262 3805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2836 3134 1243
Size: 879 714 298 2176
Fragments arranged by size:
2176 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 819 1420 819
Size: 601 3466
BsaBI GATnn'nnATC
Cuts at: 6 358 418 595 1581 3618 6
Size: 352 60 177 986 2037 455
Fragments arranged by size:
2037 986 455 352 177 60
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2866 3013 3287 1256
Size: 521 678 411 147 274 2036
Fragments arranged by size:
2036 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 713 743 713
Size: 30 4037
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 867 867
Size: 4067
BseYI C'CCAG_C
Cuts at: 407 600 1492 2250 2915 3323 407
Size: 193 892 758 665 408 1151
Fragments arranged by size:
1151 892 758 665 408 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 400 406 226
Size: 174 6 3887
Fragments arranged by size:
3887 174 6
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2885 104
Size: 1505 46 1230 1286
Fragments arranged by size:
1505 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 624 1081 1859 2049 2909 624
Size: 457 778 190 860 1782
Fragments arranged by size:
1782 860 778 457 190
BsmI GAATG_Cn'
Cuts at: 828 828
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 624 1081 1692 1785 1859 2049 2111 2909 624
Size: 457 611 93 74 190 62 798 1782
Fragments arranged by size:
1782 798 611 457 190 93 74 62
BspHI T'CATG_A
Cuts at: 731 2499 731
Size: 1768 2299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 171 198 372 923 1117 1331 1633 2014 171
Size: 27 174 551 194 214 302 381 2224
Fragments arranged by size:
2224 551 381 302 214 194 174 27
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 711 1043 1979 711
Size: 332 936 2799
Fragments arranged by size:
2799 936 332
BsrFI r'CCGG_y
Cuts at: 2065 2246 2065
Size: 181 3886
BssSI C'ACGA_G
Cuts at: 2338 3046 2338
Size: 708 3359
BstAPI GCAn_nnn'nTGC
Cuts at: 505 1444 505
Size: 939 3128
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstYI r'GATC_y
Cuts at: 1 353 359 1011 1092 1559 1582 1917 2163
Size: 352 6 652 81 467 23 335 246
Cuts at: 2163 2469 2481 2567 3619 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 652 467 449 352 335 306 246
86 81 23 12 6
BstZ17I GTA'TAC
Cuts at: 119 119
Size: 4067
BtgI C'CryG_G
Cuts at: 107 941 107
Size: 834 3233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 443 669 1310 443
Size: 226 641 3200
Fragments arranged by size:
3200 641 226
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 3117 1773
Size: 1344 2723
EaeI y'GGCC_r
Cuts at: 240 450 1652 1826 2217 2244 3589 240
Size: 210 1202 174 391 27 1345 718
Fragments arranged by size:
1345 1202 718 391 210 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 800 2090 2300 800
Size: 1290 210 2567
Fragments arranged by size:
2567 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 3017 3163 3877 2275
Size: 742 146 714 2465
Fragments arranged by size:
2465 742 714 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 544 806 1892 2324 2677 3339 530
Size: 14 262 1086 432 353 662 1258
Fragments arranged by size:
1258 1086 662 432 353 262 14
EcoNI CCTnn'n_nnAGG
Cuts at: 581 581
Size: 4067
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2979 3750 1501
Size: 248 1230 771 1818
Fragments arranged by size:
1818 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 713 745 1909 1939 1941 1971 713
Size: 32 1164 30 2 30 2809
Fragments arranged by size:
2809 1164 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2826 3010 2826
Size: 184 3883
MscI TGG'CCA
Cuts at: 242 452 1828 242
Size: 210 1376 2481
Fragments arranged by size:
2481 1376 210
MslI CAynn'nnrTG
Cuts at: 935 1054 2056 2183 935
Size: 119 1002 127 2819
Fragments arranged by size:
2819 1002 127 119
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2636 2881 1178
Size: 314 360 784 245 2364
Fragments arranged by size:
2364 784 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 941 107
Size: 834 3233
NdeI CA'TA_TG
Cuts at: 1004 1004
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 3223 1120
Size: 500 1603 1964
Fragments arranged by size:
1964 1603 500
PciI A'CATG_T
Cuts at: 3219 3219
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 4035
PshAI GACnn'nnGTC
Cuts at: 439 523 439
Size: 84 3983
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 800 2090 2300 800
Size: 1290 210 2567
Fragments arranged by size:
2567 1290 210
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 1110 1795 2763 2954 33
Size: 1077 685 968 191 1146
Fragments arranged by size:
1146 1077 968 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 398 398
Size: 4067
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 847 1222 2137 2851 3113 847
Size: 375 915 714 262 1801
Fragments arranged by size:
1801 915 714 375 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 107 186 941 1201 1555 107
Size: 79 755 260 354 2619
Fragments arranged by size:
2619 755 354 260 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 479 511 942 974 1034 1066 3584
Size: 32 85 32 431 32 60 32 2518
Cuts at: 3584 3616 362
Size: 32 813
Fragments arranged by size:
2518 813 431 85 60 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 278 1864 278
Size: 1586 2481
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 265 364 1562 265
Size: 99 1198 2770
Fragments arranged by size:
2770 1198 99
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI