Restriction map for
pF1KB9562
(Circular) (Six-base) MAPSORT of: pF1KB9562.seq Check: 8344 from: 1 to: 5477
pF1KB9562 5477 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 926 1640 2202 2217 926
Size: 714 562 15 4186
Fragments arranged by size:
4186 714 562 15
AatII G_ACGT'C
Cuts at: 629 1292 629
Size: 663 4814
AccI GT'mk_AC
Cuts at: 115 1760 2515 115
Size: 1645 755 3077
Fragments arranged by size:
3077 1645 755
Acc65I G'GTAC_C
Cuts at: 2493 2493
Size: 5477
AclI AA'CG_TT
Cuts at: 5317 5317
Size: 5477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 3302 3734 4087 4749 1297
Size: 2005 432 353 662 2025
Fragments arranged by size:
2025 2005 662 432 353
AflIII A'CryG_T
Cuts at: 1577 2387 2829 4629 4910 1577
Size: 810 442 1800 281 2144
Fragments arranged by size:
2144 1800 810 442 281
AleI CACnn'nnGTG
Cuts at: 232 415 992 1370 1567 2302 2443 232
Size: 183 577 378 197 735 141 3266
Fragments arranged by size:
3266 735 577 378 197 183 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 421
Size: 5477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1108 1110 1140 1078
Size: 30 2 30 5415
Fragments arranged by size:
5415 30 30 2
AlwNI CAG_nnn'CTG
Cuts at: 1069 1218 2217 2782 4220 1069
Size: 149 999 565 1438 2326
Fragments arranged by size:
2326 1438 999 565 149
ApaLI G'TGCA_C
Cuts at: 4315 4315
Size: 5477
ApoI r'AATT_y
Cuts at: 1258 2481 4830 5086 1258
Size: 1223 2349 256 1649
Fragments arranged by size:
2349 1649 1223 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5477
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
AvaI C'yCGr_G
Cuts at: 475 1334 1349 2358 2497 475
Size: 859 15 1009 139 3455
Fragments arranged by size:
3455 1009 859 139 15
BamHI G'GATC_C
Cuts at: 902 2502 902
Size: 1600 3877
BanI G'GyrC_C
Cuts at: 255 1024 1036 1526 1847 1894 1939 2014 2296
Size: 769 12 490 321 47 45 75 282
Cuts at: 2296 2493 2907 3155 3190 255
Size: 197 414 248 35 2542
Fragments arranged by size:
2542 769 490 414 321 282 248 197
75 47 45 35 12
BanII G_rGCy'C
Cuts at: 1868 1912 2042 2084 2146 2491 3521 1868
Size: 44 130 42 62 345 1030 3824
Fragments arranged by size:
3824 1030 345 130 62 44 42
BbeI G_GCGC'C
Cuts at: 1851 2911 3159 1851
Size: 1060 248 4169
Fragments arranged by size:
4169 1060 248
BbsI GAAGACnn'nnnn_
Cuts at: 1368 1458 1686 1704 1368
Size: 90 228 18 5141
Fragments arranged by size:
5141 228 90 18
BciVI GTATCCnnnnn_n'
Cuts at: 3375 4431 5477 3375
Size: 1056 1046 3375
Fragments arranged by size:
3375 1056 1046
BclI T'GATC_A
Cuts at: 2997 2997
Size: 5477
BglII A'GATC_T
Cuts at: 2237 2992 5029 2237
Size: 755 2037 2685
Fragments arranged by size:
2685 2037 755
BlpI GC'TnA_GC
Cuts at: 2589 2589
Size: 5477
Bme1580I G_kGCm'C
Cuts at: 476 1451 1529 1897 2324 2365 3102 3195 4319
Size: 975 78 368 427 41 737 93 1124
Cuts at: 4319 476
Size: 1634
Fragments arranged by size:
1634 1124 975 737 427 368 93 78
41
BmgBI CAC'GTC
Cuts at: 2445 2445
Size: 5477
BmrI ACTGGGnnnn_n'
Cuts at: 1456 2689 2770 3105 5249 1456
Size: 1233 81 335 2144 1684
Fragments arranged by size:
2144 1684 1233 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 1662 1914 3844 312
Size: 1350 252 1930 1945
Fragments arranged by size:
1945 1930 1350 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1046 1153 1657 1726 1952 468
Size: 578 107 504 69 226 3993
Fragments arranged by size:
3993 578 504 226 107 69
Bpu10I CC'TnA_GC
Cuts at: 1862 2036 2072 1862
Size: 174 36 5267
Fragments arranged by size:
5267 174 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1576 1930 2653 3532 4246 4544 1117
Size: 459 354 723 879 714 298 2050
Fragments arranged by size:
2050 879 723 714 459 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 5181 5181
Size: 5477
BsaAI yAC'GTr
Cuts at: 2830 2830
Size: 5477
BsaBI GATnn'nnATC
Cuts at: 6 1055 2242 2991 5028 6
Size: 1049 1187 749 2037 455
Fragments arranged by size:
2037 1187 1049 749 455
BsaHI Gr'CG_yC
Cuts at: 626 1289 1848 2396 2908 3156 3858 5354 626
Size: 663 559 548 512 248 702 1496 749
Fragments arranged by size:
1496 749 702 663 559 548 512 248
BsaWI w'CCGG_w
Cuts at: 137 596 716 780 2666 3187 3865 4276 4423
Size: 459 120 64 1886 521 678 411 147
Cuts at: 4423 4697 137
Size: 274 917
Fragments arranged by size:
1886 917 678 521 459 411 274 147
120 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 616 646 616
Size: 30 5447
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 503 615 853 967 1126 503
Size: 112 238 114 159 4854
Fragments arranged by size:
4854 238 159 114 112
BseYI C'CCAG_C
Cuts at: 689 774 2042 2083 2902 3660 4325 4733 689
Size: 85 1268 41 819 758 665 408 1433
Fragments arranged by size:
1433 1268 819 758 665 408 85 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 361 1612 256
Size: 105 1251 4121
Fragments arranged by size:
4121 1251 105
BsiEI CG_ry'CG
Cuts at: 104 119 2113 3019 3065 4295 104
Size: 15 1994 906 46 1230 1286
Fragments arranged by size:
1994 1286 1230 906 46 15
BsiHKAI G_wGCw'C
Cuts at: 607 971 2491 3269 3459 4319 607
Size: 364 1520 778 190 860 1765
Fragments arranged by size:
1765 1520 860 778 364 190
Bsp1286I G_dGCh'C
Cuts at: 476 607 971 1451 1529 1868 1897 1912 2042
Size: 131 364 480 78 339 29 15 130
Cuts at: 2042 2084 2146 2324 2365 2491 3102 3195 3269
Size: 42 62 178 41 126 611 93 74
Cuts at: 3269 3459 3521 4319 476
Size: 190 62 798 1634
Fragments arranged by size:
1634 798 611 480 364 339 190 178
131 130 126 93 78 74 62 62
42 41 29 15
BspEI T'CCGG_A
Cuts at: 137 780 137
Size: 643 4834
BspHI T'CATG_A
Cuts at: 2198 3909 2198
Size: 1711 3766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 569 776 926 1187 1496 1640 1814 2202 2217
Size: 207 150 261 309 144 174 388 15
Cuts at: 2217 2527 2741 3043 3424 569
Size: 310 214 302 381 2622
Fragments arranged by size:
2622 388 381 310 309 302 261 214
207 174 150 144 15
BsrBI CCG'CTC
Cuts at: 50 1397 1404 3769 3823 5307 5472 50
Size: 1347 7 2365 54 1484 165 55
Fragments arranged by size:
2365 1484 1347 165 55 54 7
BsrDI GCAATG_nn'
Cuts at: 551 3389 551
Size: 2838 2639
BsrFI r'CCGG_y
Cuts at: 258 401 1850 1879 3475 3656 258
Size: 143 1449 29 1596 181 2079
Fragments arranged by size:
2079 1596 1449 181 143 29
BssSI C'ACGA_G
Cuts at: 3748 4456 3748
Size: 708 4769
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 2854
Size: 5477
BstBI TT'CG_AA
Cuts at: 3838 3838
Size: 5477
BstEII G'GTnAC_C
Cuts at: 1608 1608
Size: 5477
BstXI CCAn_nnnn'nTGG
Cuts at: 528 1741 1959 528
Size: 1213 218 4046
Fragments arranged by size:
4046 1213 218
BstYI r'GATC_y
Cuts at: 1 269 902 1199 2237 2273 2502 2969 2992
Size: 268 633 297 1038 36 229 467 23
Cuts at: 2992 3327 3573 3879 3891 3977 5029 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1038 633 467 449 335 306 297
268 246 229 86 36 23 12
BtgI C'CryG_G
Cuts at: 107 176 802 824 1043 1451 1604 1811 1901
Size: 69 626 22 219 408 153 207 90
Cuts at: 1901 107
Size: 3683
Fragments arranged by size:
3683 626 408 219 207 153 90 69
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3427 3608 3771 3427
Size: 181 163 5133
Fragments arranged by size:
5133 181 163
BtsI GCAGTG_nn'
Cuts at: 1008 2143 2363 2720 1008
Size: 1135 220 357 3765
Fragments arranged by size:
3765 1135 357 220
DraI TTT'AAA
Cuts at: 2476 2476
Size: 5477
DraIII CAC_nnn'GTG
Cuts at: 314 875 314
Size: 561 4916
DrdI GACnn_nn'nnGTC
Cuts at: 1303 1462 1992 2265 2425 3183 4527 1303
Size: 159 530 273 160 758 1344 2253
Fragments arranged by size:
2253 1344 758 530 273 160 159
EaeI y'GGCC_r
Cuts at: 305 530 548 1799 3062 3236 3627 3654 4999
Size: 225 18 1251 1263 174 391 27 1345
Cuts at: 4999 305
Size: 783
Fragments arranged by size:
1345 1263 1251 783 391 225 174 27
18
EagI C'GGCC_G
Cuts at: 3062 3062
Size: 5477
EarI CTCTTCn'nnn_
Cuts at: 281 497 571 856 928 1234 1270 1273 1321
Size: 216 74 285 72 306 36 3 48
Cuts at: 1321 1552 1669 3500 3710 281
Size: 231 117 1831 210 2048
Fragments arranged by size:
2048 1831 306 285 231 216 210 117
74 72 48 36 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1869 3685 4427 4573 5287 1869
Size: 1816 742 146 714 2059
Fragments arranged by size:
2059 1816 742 714 146
EcoICRI GAG'CTC
Cuts at: 2489 2489
Size: 5477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1046 1153 1297 1657 1726 1952 3302 3734
Size: 578 107 144 360 69 226 1350 432
Cuts at: 3734 4087 4749 468
Size: 353 662 1196
Fragments arranged by size:
1350 1196 662 578 432 360 353 226
144 107 69
EcoNI CCTnn'n_nnAGG
Cuts at: 949 1636 1705 949
Size: 687 69 4721
Fragments arranged by size:
4721 687 69
EcoO109I rG'GnC_Cy
Cuts at: 438 524 1066 1475 1836 2160 2253 2616 438
Size: 86 542 409 361 324 93 363 3299
Fragments arranged by size:
3299 542 409 363 361 324 93 86
EcoRI G'AATT_C
Cuts at: 2481 2481
Size: 5477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 3496 150
Size: 3346 2131
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5477
HaeII r_GCGC'y
Cuts at: 1851 2911 3159 4389 5160 1851
Size: 1060 248 1230 771 2168
Fragments arranged by size:
2168 1230 1060 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 616 648 1732 1764 1855 1887 3319 3349 3351
Size: 32 1084 32 91 32 1432 30 2
Cuts at: 3351 3381 616
Size: 30 2712
Fragments arranged by size:
2712 1432 1084 91 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 116 1575 2516 116
Size: 1459 941 3077
Fragments arranged by size:
3077 1459 941
KasI G'GCGC_C
Cuts at: 1847 2907 3155 1847
Size: 1060 248 4169
Fragments arranged by size:
4169 1060 248
KpnI G_GTAC'C
Cuts at: 2497 2497
Size: 5477
MluI A'CGCG_T
Cuts at: 4910 4910
Size: 5477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 1181 1775 4236 4420 323
Size: 858 594 2461 184 1380
Fragments arranged by size:
2461 1380 858 594 184
MscI TGG'CCA
Cuts at: 307 550 1801 3238 307
Size: 243 1251 1437 2546
Fragments arranged by size:
2546 1437 1251 243
MslI CAynn'nnrTG
Cuts at: 232 415 992 1042 1370 1498 1567 1900 2302
Size: 183 577 50 328 128 69 333 402
Cuts at: 2302 2368 2386 2443 3466 3593 232
Size: 66 18 57 1023 127 2116
Fragments arranged by size:
2116 1023 577 402 333 328 183 128
127 69 66 57 50 18
MspA1I CmG'CkG
Cuts at: 644 1523 1533 1797 1845 1921 2106 2187 2466
Size: 879 10 264 48 76 185 81 279
Cuts at: 2466 2588 2902 3262 4046 4291 644
Size: 122 314 360 784 245 1830
Fragments arranged by size:
1830 879 784 360 314 279 264 245
185 122 81 76 48 10
NaeI GCC'GGC
Cuts at: 260 403 1852 3658 260
Size: 143 1449 1806 2079
Fragments arranged by size:
2079 1806 1449 143
NarI GG'CG_CC
Cuts at: 1848 2908 3156 1848
Size: 1060 248 4169
Fragments arranged by size:
4169 1060 248
NcoI C'CATG_G
Cuts at: 107 802 1043 1451 1604 1901 107
Size: 695 241 408 153 297 3683
Fragments arranged by size:
3683 695 408 297 241 153
NgoMIV G'CCGG_C
Cuts at: 258 401 1850 3656 258
Size: 143 1449 1806 2079
Fragments arranged by size:
2079 1806 1449 143
NspI r_CATG'y
Cuts at: 520 749 841 2391 2530 3030 4633 520
Size: 229 92 1550 139 500 1603 1364
Fragments arranged by size:
1603 1550 1364 500 229 139 92
PciI A'CATG_T
Cuts at: 2387 4629 2387
Size: 2242 3235
PflMI CCAn_nnn'nTGG
Cuts at: 636 803 1655 1689 1797 636
Size: 167 852 34 108 4316
Fragments arranged by size:
4316 852 167 108 34
PfoI T'CCnGG_A
Cuts at: 1703 2167 1703
Size: 464 5013
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 5445
PpuMI rG'GwC_Cy
Cuts at: 438 1475 2160 438
Size: 1037 685 3755
Fragments arranged by size:
3755 1037 685
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1417 1449 1417
Size: 32 5445
PstI C_TGCA'G
Cuts at: 400 1844 1932 2192 2213 2291 2524 3209 400
Size: 1444 88 260 21 78 233 685 2668
Fragments arranged by size:
2668 1444 685 260 233 88 78 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5477
PvuII CAG'CTG
Cuts at: 644 1797 2902 3262 644
Size: 1153 1105 360 2859
Fragments arranged by size:
2859 1153 1105 360
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5477
SacI G_AGCT'C
Cuts at: 2491 2491
Size: 5477
SalI G'TCGA_C
Cuts at: 114 2514 114
Size: 2400 3077
SanDI GG'GwC_CC
Cuts at: 1475 1475
Size: 5477
SapI GCTCTTCn'nnn_
Cuts at: 571 928 1273 3500 3710 571
Size: 357 345 2227 210 2338
Fragments arranged by size:
2338 2227 357 345 210
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5477
ScaI AGT'ACT
Cuts at: 4994 4994
Size: 5477
SexAI A'CCwGG_T
Cuts at: 1654 1688 1754 1654
Size: 34 66 5377
Fragments arranged by size:
5377 66 34
SfcI C'TryA_G
Cuts at: 33 396 1840 1928 2188 2209 2287 2520 3205
Size: 363 1444 88 260 21 78 233 685
Cuts at: 3205 4173 4364 33
Size: 968 191 1146
Fragments arranged by size:
1444 1146 968 685 363 260 233 191
88 78 21
SfoI GGC'GCC
Cuts at: 1849 2909 3157 1849
Size: 1060 248 4169
Fragments arranged by size:
4169 1060 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
SgrAI Cr'CCGG_yG
Cuts at: 1850 1850
Size: 5477
SmaI CCC'GGG
Cuts at: 477 1336 2499 477
Size: 859 1163 3455
Fragments arranged by size:
3455 1163 859
SmlI C'TyrA_G
Cuts at: 1132 1591 1945 2632 3547 4261 4523 1132
Size: 459 354 687 915 714 262 2086
Fragments arranged by size:
2086 915 714 687 459 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5477
SphI G_CATG'C
Cuts at: 520 2530 3030 520
Size: 2010 500 2967
Fragments arranged by size:
2967 2010 500
SrfI GCCC'GGGC
Cuts at: 1336 1336
Size: 5477
StuI AGG'CCT
Cuts at: 1642 1642
Size: 5477
StyI C'CwwG_G
Cuts at: 107 802 1043 1451 1604 1901 2611 2965 107
Size: 695 241 408 153 297 710 354 2619
Fragments arranged by size:
2619 710 695 408 354 297 241 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 133 2002 3060 3193 3652 3861 133
Size: 1869 1058 133 459 209 1749
Fragments arranged by size:
1869 1749 1058 459 209 133
TatI w'GTAC_w
Cuts at: 4992 4992
Size: 5477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4994 5026 4994
Size: 32 5445
Tth111I GACn'n_nGTC
Cuts at: 3274 3274
Size: 5477
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 2153 2972 1014
Size: 1139 819 3519
Fragments arranged by size:
3519 1139 819
XmaI C'CCGG_G
Cuts at: 475 1334 2497 475
Size: 859 1163 3455
Fragments arranged by size:
3455 1163 859
ZraI GAC'GTC
Cuts at: 627 1290 627
Size: 663 4814
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
SacII SfiI SnaBI SspI SwaI XhoI XmnI