Restriction map for
pF1KB9560
(Circular) (Six-base) MAPSORT of: pF1KB9560.seq Check: 1215 from: 1 to: 6521
pF1KB9560 6521 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 3559 3559
Size: 6521
Acc65I G'GTAC_C
Cuts at: 1958 2345 3537 1958
Size: 387 1192 4942
Fragments arranged by size:
4942 1192 387
AclI AA'CG_TT
Cuts at: 6361 6361
Size: 6521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1620 1992 2446 2533 2673 2680 2713 3098
Size: 844 372 454 87 140 7 33 385
Cuts at: 3098 3285 4346 4778 5131 5793 776
Size: 187 1061 432 353 662 1504
Fragments arranged by size:
1504 1061 844 662 454 432 385 372
353 187 140 87 33 7
AfeI AGC'GCT
Cuts at: 220 2440 220
Size: 2220 4301
AflII C'TTAA_G
Cuts at: 775 775
Size: 6521
AflIII A'CryG_T
Cuts at: 1613 3472 3873 5673 5954 1613
Size: 1859 401 1800 281 2180
Fragments arranged by size:
2180 1859 1800 401 281
AgeI A'CCGG_T
Cuts at: 281 281
Size: 6521
AhdI GACnn_n'nnGTC
Cuts at: 3168 3168
Size: 6521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2645 2645
Size: 6521
AlwNI CAG_nnn'CTG
Cuts at: 1519 2632 3826 5264 1519
Size: 1113 1194 1438 2776
Fragments arranged by size:
2776 1438 1194 1113
ApaLI G'TGCA_C
Cuts at: 5359 5359
Size: 6521
ApoI r'AATT_y
Cuts at: 333 625 855 1031 2123 2411 2683 3525 5874
Size: 292 230 176 1092 288 272 842 2349
Cuts at: 5874 6130 333
Size: 256 724
Fragments arranged by size:
2349 1092 842 724 292 288 272 256
230 176
AseI AT'TA_AT
Cuts at: 20 1319 20
Size: 1299 5222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6521
AvaI C'yCGr_G
Cuts at: 686 977 3541 686
Size: 291 2564 3666
Fragments arranged by size:
3666 2564 291
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3200 3233 3200
Size: 33 6488
BamHI G'GATC_C
Cuts at: 1640 3546 1640
Size: 1906 4615
BanI G'GyrC_C
Cuts at: 748 1958 2345 3537 3951 4199 4234 748
Size: 1210 387 1192 414 248 35 3035
Fragments arranged by size:
3035 1210 1192 414 387 248 35
BanII G_rGCy'C
Cuts at: 352 764 3229 3271 3535 4565 352
Size: 412 2465 42 264 1030 2308
Fragments arranged by size:
2465 2308 1030 412 264 42
BbeI G_GCGC'C
Cuts at: 3955 4203 3955
Size: 248 6273
BbsI GAAGACnn'nnnn_
Cuts at: 2204 2204
Size: 6521
BciVI GTATCCnnnnn_n'
Cuts at: 4419 5475 6521 4419
Size: 1056 1046 4419
Fragments arranged by size:
4419 1056 1046
BclI T'GATC_A
Cuts at: 1053 3309 4041 1053
Size: 2256 732 3533
Fragments arranged by size:
3533 2256 732
BglII A'GATC_T
Cuts at: 4036 6073 4036
Size: 2037 4484
BlpI GC'TnA_GC
Cuts at: 3633 3633
Size: 6521
Bme1580I G_kGCm'C
Cuts at: 4146 4239 5363 4146
Size: 93 1124 5304
Fragments arranged by size:
5304 1124 93
BmgBI CAC'GTC
Cuts at: 1616 1616
Size: 6521
BmrI ACTGGGnnnn_n'
Cuts at: 437 958 3733 3814 4149 6293 437
Size: 521 2775 81 335 2144 665
Fragments arranged by size:
2775 2144 665 521 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3313 4888 3137
Size: 176 1575 4770
Fragments arranged by size:
4770 1575 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2239 2239
Size: 6521
Bpu10I CC'TnA_GC
Cuts at: 1136 1136
Size: 6521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 802 2793 3697 4576 5290 5588 196
Size: 606 1991 904 879 714 298 1129
Fragments arranged by size:
1991 1129 904 879 714 606 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 1519 1709 6225 187
Size: 1332 190 4516 483
Fragments arranged by size:
4516 1332 483 190
BsaAI yAC'GTr
Cuts at: 2668 3874 2668
Size: 1206 5315
BsaBI GATnn'nnATC
Cuts at: 6 2164 4035 6072 6
Size: 2158 1871 2037 455
Fragments arranged by size:
2158 2037 1871 455
BsaHI Gr'CG_yC
Cuts at: 3952 4200 4902 6398 3952
Size: 248 702 1496 4075
Fragments arranged by size:
4075 1496 702 248
BsaWI w'CCGG_w
Cuts at: 281 3710 4231 4909 5320 5467 5741 281
Size: 3429 521 678 411 147 274 1061
Fragments arranged by size:
3429 1061 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 494 524 494
Size: 30 6491
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 585 668 754 1396 2533 2620 205
Size: 380 83 86 642 1137 87 4106
Fragments arranged by size:
4106 1137 642 380 87 86 83
BseYI C'CCAG_C
Cuts at: 3946 4704 5369 5777 3946
Size: 758 665 408 4690
Fragments arranged by size:
4690 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 4063 4109 5339 104
Size: 3959 46 1230 1286
Fragments arranged by size:
3959 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 764 1597 3229 3535 4313 4503 5363 764
Size: 833 1632 306 778 190 860 1922
Fragments arranged by size:
1922 1632 860 833 778 306 190
BsmI GAATG_Cn'
Cuts at: 879 1075 879
Size: 196 6325
BsmBI CGTCTCn'nnnn_
Cuts at: 1622 2499 3499 1622
Size: 877 1000 4644
Fragments arranged by size:
4644 1000 877
Bsp1286I G_dGCh'C
Cuts at: 352 764 1597 3229 3271 3535 4146 4239 4313
Size: 412 833 1632 42 264 611 93 74
Cuts at: 4313 4503 4565 5363 352
Size: 190 62 798 1510
Fragments arranged by size:
1632 1510 833 798 611 412 264 190
93 74 62 42
BspHI T'CATG_A
Cuts at: 903 4953 903
Size: 4050 2471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 788 1172 3571 3785 4087 4468 788
Size: 384 2399 214 302 381 2841
Fragments arranged by size:
2841 2399 384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4813 4867 6351 6516 50
Size: 4763 54 1484 165 55
Fragments arranged by size:
4763 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 3071 4433 2462
Size: 609 1362 4550
Fragments arranged by size:
4550 1362 609
BsrFI r'CCGG_y
Cuts at: 281 1045 4519 4700 281
Size: 764 3474 181 2102
Fragments arranged by size:
3474 2102 764 181
BssSI C'ACGA_G
Cuts at: 4792 5500 4792
Size: 708 5813
BstAPI GCAn_nnn'nTGC
Cuts at: 3898 3898
Size: 6521
BstBI TT'CG_AA
Cuts at: 4882 4882
Size: 6521
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3457 3172
Size: 285 6236
BstYI r'GATC_y
Cuts at: 1 385 1640 2569 3546 4013 4036 4371 4617
Size: 384 1255 929 977 467 23 335 246
Cuts at: 4617 4923 4935 5021 6073 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1255 1052 977 929 467 449 384 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2203 2203
Size: 6521
BtgI C'CryG_G
Cuts at: 107 1838 2129 107
Size: 1731 291 4499
Fragments arranged by size:
4499 1731 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4471 4652 4815 4471
Size: 181 163 6177
Fragments arranged by size:
6177 181 163
BtsI GCAGTG_nn'
Cuts at: 944 3312 3764 944
Size: 2368 452 3701
Fragments arranged by size:
3701 2368 452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1714 1749 1714
Size: 35 6486
DraI TTT'AAA
Cuts at: 636 3520 636
Size: 2884 3637
DrdI GACnn_nn'nnGTC
Cuts at: 4227 5571 4227
Size: 1344 5177
EaeI y'GGCC_r
Cuts at: 110 824 1478 1841 2228 3326 4106 4280 4671
Size: 714 654 363 387 1098 780 174 391
Cuts at: 4671 4698 6043 110
Size: 27 1345 588
Fragments arranged by size:
1345 1098 780 714 654 588 391 387
363 174 27
EagI C'GGCC_G
Cuts at: 4106 4106
Size: 6521
EarI CTCTTCn'nnn_
Cuts at: 752 1367 1487 2918 2953 3106 3304 4544 4754
Size: 615 120 1431 35 153 198 1240 210
Cuts at: 4754 752
Size: 2519
Fragments arranged by size:
2519 1431 1240 615 210 198 153 120
35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2355 4729 5471 5617 6331 2355
Size: 2374 742 146 714 2545
Fragments arranged by size:
2545 2374 742 714 146
EcoICRI GAG'CTC
Cuts at: 762 3227 3533 762
Size: 2465 306 3750
Fragments arranged by size:
3750 2465 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1620 1992 2239 2446 2533 2673 2680 2713
Size: 844 372 247 207 87 140 7 33
Cuts at: 2713 3098 3285 4346 4778 5131 5793 776
Size: 385 187 1061 432 353 662 1504
Fragments arranged by size:
1504 1061 844 662 432 385 372 353
247 207 187 140 87 33 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1814 2319 3262 1814
Size: 505 943 5073
Fragments arranged by size:
5073 943 505
EcoO109I rG'GnC_Cy
Cuts at: 2103 2483 3660 2103
Size: 380 1177 4964
Fragments arranged by size:
4964 1177 380
EcoRI G'AATT_C
Cuts at: 3525 3525
Size: 6521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 237 547 4540 237
Size: 310 3993 2218
Fragments arranged by size:
3993 2218 310
FspI TGC'GCA
Cuts at: 2333 4302 2333
Size: 1969 4552
HaeII r_GCGC'y
Cuts at: 222 2442 3955 4203 5433 6204 222
Size: 2220 1513 248 1230 771 539
Fragments arranged by size:
2220 1513 1230 771 539 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 3154 3186 4363 4393 4395 4425 580
Size: 32 2542 32 1177 30 2 30 2676
Fragments arranged by size:
2676 2542 1177 32 32 30 30 2
HincII GTy'rAC
Cuts at: 273 1689 3560 273
Size: 1416 1871 3234
Fragments arranged by size:
3234 1871 1416
HindIII A'AGCT_T
Cuts at: 463 557 778 463
Size: 94 221 6206
Fragments arranged by size:
6206 221 94
HpaI GTT'AAC
Cuts at: 273 273
Size: 6521
KasI G'GCGC_C
Cuts at: 3951 4199 3951
Size: 248 6273
KpnI G_GTAC'C
Cuts at: 1962 2349 3541 1962
Size: 387 1192 4942
Fragments arranged by size:
4942 1192 387
MluI A'CGCG_T
Cuts at: 5954 5954
Size: 6521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 2377 5280 5464 566
Size: 1811 2903 184 1623
Fragments arranged by size:
2903 1811 1623 184
MscI TGG'CCA
Cuts at: 112 1480 1843 2230 3328 4282 112
Size: 1368 363 387 1098 954 2351
Fragments arranged by size:
2351 1368 1098 954 387 363
MslI CAynn'nnrTG
Cuts at: 1849 4510 4637 1849
Size: 2661 127 3733
Fragments arranged by size:
3733 2661 127
MspA1I CmG'CkG
Cuts at: 487 738 1631 3632 3946 4306 5090 5335 487
Size: 251 893 2001 314 360 784 245 1673
Fragments arranged by size:
2001 1673 893 784 360 314 251 245
NaeI GCC'GGC
Cuts at: 4702 4702
Size: 6521
NarI GG'CG_CC
Cuts at: 3952 4200 3952
Size: 248 6273
NcoI C'CATG_G
Cuts at: 107 107
Size: 6521
NgoMIV G'CCGG_C
Cuts at: 4700 4700
Size: 6521
NspI r_CATG'y
Cuts at: 2539 2626 3476 3574 4074 5677 2539
Size: 87 850 98 500 1603 3383
Fragments arranged by size:
3383 1603 850 500 98 87
PciI A'CATG_T
Cuts at: 3472 5673 3472
Size: 2201 4320
PfoI T'CCnGG_A
Cuts at: 551 551
Size: 6521
PmeI GTTT'AAAC
Cuts at: 3520 3520
Size: 6521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4967 4999 4967
Size: 32 6489
PpuMI rG'GwC_Cy
Cuts at: 2103 2483 2103
Size: 380 6141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 518 550 518
Size: 32 6489
PstI C_TGCA'G
Cuts at: 3568 4253 3568
Size: 685 5836
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6521
PvuII CAG'CTG
Cuts at: 487 738 1631 3946 4306 487
Size: 251 893 2315 360 2702
Fragments arranged by size:
2702 2315 893 360 251
RsrII CG'GwC_CG
Cuts at: 4716 4716
Size: 6521
SacI G_AGCT'C
Cuts at: 764 3229 3535 764
Size: 2465 306 3750
Fragments arranged by size:
3750 2465 306
SalI G'TCGA_C
Cuts at: 3558 3558
Size: 6521
SanDI GG'GwC_CC
Cuts at: 2483 2483
Size: 6521
SapI GCTCTTCn'nnn_
Cuts at: 752 1367 4544 4754 752
Size: 615 3177 210 2519
Fragments arranged by size:
3177 2519 615 210
SbfI CC_TGCA'GG
Cuts at: 3568 3568
Size: 6521
ScaI AGT'ACT
Cuts at: 6038 6038
Size: 6521
SexAI A'CCwGG_T
Cuts at: 1670 1670
Size: 6521
SfcI C'TryA_G
Cuts at: 33 1176 1811 1861 3564 4249 5217 5408 33
Size: 1143 635 50 1703 685 968 191 1146
Fragments arranged by size:
1703 1146 1143 968 685 635 191 50
SfoI GGC'GCC
Cuts at: 3953 4201 3953
Size: 248 6273
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6521
SmaI CCC'GGG
Cuts at: 3543 3543
Size: 6521
SmlI C'TyrA_G
Cuts at: 211 775 781 977 2808 3676 4591 5305 5567
Size: 564 6 196 1831 868 915 714 262
Cuts at: 5567 211
Size: 1165
Fragments arranged by size:
1831 1165 915 868 714 564 262 196
6
SnaBI TAC'GTA
Cuts at: 2668 2668
Size: 6521
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6521
SphI G_CATG'C
Cuts at: 2626 3574 4074 2626
Size: 948 500 5073
Fragments arranged by size:
5073 948 500
SspI AAT'ATT
Cuts at: 3378 3378
Size: 6521
StuI AGG'CCT
Cuts at: 1806 2017 1806
Size: 211 6310
StyI C'CwwG_G
Cuts at: 107 2997 3212 3655 4009 107
Size: 2890 215 443 354 2619
Fragments arranged by size:
2890 2619 443 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1246 1576 4104 4237 4696 4905 1246
Size: 330 2528 133 459 209 2862
Fragments arranged by size:
2862 2528 459 330 209 133
TatI w'GTAC_w
Cuts at: 3115 6036 3115
Size: 2921 3600
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1480 1512 6038 6070 1480
Size: 32 4526 32 1931
Fragments arranged by size:
4526 1931 32 32
Tth111I GACn'n_nGTC
Cuts at: 4318 4318
Size: 6521
XbaI T'CTAG_A
Cuts at: 598 990 1065 3552 598
Size: 392 75 2487 3567
Fragments arranged by size:
3567 2487 392 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 549 885 1651 2094 4016 114
Size: 435 336 766 443 1922 2619
Fragments arranged by size:
2619 1922 766 443 435 336
XhoI C'TCGA_G
Cuts at: 977 977
Size: 6521
XmaI C'CCGG_G
Cuts at: 3541 3541
Size: 6521
XmnI GAAnn'nnTTC
Cuts at: 2686 2686
Size: 6521
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AleI AloI ApaI AscI AvrII BbvCI
BcgI BfrBI BglI BmtI BsgI BsiWI BspEI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI SacII SfiI
SgrAI SrfI SwaI ZraI