Restriction map for
pF1KB9556
(Circular) (Six-base) MAPSORT of: pF1KB9556.seq Check: 9686 from: 1 to: 5726
pF1KB9556 5726 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2529 2529
Size: 5726
AatII G_ACGT'C
Cuts at: 2552 2552
Size: 5726
AccI GT'mk_AC
Cuts at: 1718 2150 2764 1718
Size: 432 614 4680
Fragments arranged by size:
4680 614 432
Acc65I G'GTAC_C
Cuts at: 2742 2742
Size: 5726
AclI AA'CG_TT
Cuts at: 5566 5566
Size: 5726
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3551 3983 4730 4998 3551
Size: 432 747 268 4279
Fragments arranged by size:
4279 747 432 268
AflII C'TTAA_G
Cuts at: 2229 2229
Size: 5726
AflIII A'CryG_T
Cuts at: 2568 2653 3078 4182 5159 2568
Size: 85 425 1104 977 3135
Fragments arranged by size:
3135 1104 977 425 85
AgeI A'CCGG_T
Cuts at: 4114 4114
Size: 5726
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 734 766 1108 1140 734
Size: 32 342 32 5320
Fragments arranged by size:
5320 342 32 32
AlwNI CAG_nnn'CTG
Cuts at: 195 3031 4598 195
Size: 2836 1567 1323
Fragments arranged by size:
2836 1567 1323
ApaI G_GGCC'C
Cuts at: 725 725
Size: 5726
ApaLI G'TGCA_C
Cuts at: 4496 4496
Size: 5726
ApoI r'AATT_y
Cuts at: 1506 2029 2730 5079 5335 1506
Size: 523 701 2349 256 1897
Fragments arranged by size:
2349 1897 701 523 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
AvaI C'yCGr_G
Cuts at: 580 790 2746 580
Size: 210 1956 3560
Fragments arranged by size:
3560 1956 210
AvrII C'CTAG_G
Cuts at: 960 960
Size: 5726
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1483 1516 1483
Size: 33 5693
BamHI G'GATC_C
Cuts at: 2751 2751
Size: 5726
BanI G'GyrC_C
Cuts at: 309 361 465 615 1547 1975 2742 3156 3404
Size: 52 104 150 932 428 767 414 248
Cuts at: 3404 3439 309
Size: 35 2596
Fragments arranged by size:
2596 932 767 428 414 248 150 104
52 35
BanII G_rGCy'C
Cuts at: 235 349 713 725 788 920 1301 2326 2421
Size: 114 364 12 63 132 381 1025 95
Cuts at: 2421 2740 3770 235
Size: 319 1030 2191
Fragments arranged by size:
2191 1030 1025 381 364 319 132 114
95 63 12
BbeI G_GCGC'C
Cuts at: 365 1979 3160 3408 365
Size: 1614 1181 248 2683
Fragments arranged by size:
2683 1614 1181 248
BbsI GAAGACnn'nnnn_
Cuts at: 1160 1805 2114 1160
Size: 645 309 4772
Fragments arranged by size:
4772 645 309
BbvCI CC'TCA_GC
Cuts at: 179 179
Size: 5726
BciVI GTATCCnnnnn_n'
Cuts at: 1869 3624 4385 5726 1869
Size: 1755 761 1341 1869
Fragments arranged by size:
1869 1755 1341 761
BclI T'GATC_A
Cuts at: 3246 3246
Size: 5726
BglII A'GATC_T
Cuts at: 1484 3241 5278 1484
Size: 1757 2037 1932
Fragments arranged by size:
2037 1932 1757
BlpI GC'TnA_GC
Cuts at: 2513 2838 2513
Size: 325 5401
Bme1580I G_kGCm'C
Cuts at: 314 725 3351 3444 4500 314
Size: 411 2626 93 1056 1540
Fragments arranged by size:
2626 1540 1056 411 93
BmgBI CAC'GTC
Cuts at: 1011 1052 1011
Size: 41 5685
BmrI ACTGGGnnnn_n'
Cuts at: 213 605 2938 3019 3354 5498 213
Size: 392 2333 81 335 2144 441
Fragments arranged by size:
2333 2144 441 392 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 298 4093 298
Size: 3795 1931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2728 2267
Size: 461 5265
Bpu10I CC'TnA_GC
Cuts at: 179 179
Size: 5726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 2109 2902 3781 4273 4571 919
Size: 1190 793 879 492 298 2074
Fragments arranged by size:
2074 1190 879 793 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1031 2704 5430 1031
Size: 1673 2726 1327
Fragments arranged by size:
2726 1673 1327
BsaAI yAC'GTr
Cuts at: 3079 3079
Size: 5726
BsaBI GATnn'nnATC
Cuts at: 6 3240 5277 6
Size: 3234 2037 455
Fragments arranged by size:
3234 2037 455
BsaHI Gr'CG_yC
Cuts at: 362 1976 2549 3157 3405 4107 5603 362
Size: 1614 573 608 248 702 1496 485
Fragments arranged by size:
1614 1496 702 608 573 485 248
BsaWI w'CCGG_w
Cuts at: 2915 3436 4114 4388 4535 4946 2915
Size: 521 678 274 147 411 3695
Fragments arranged by size:
3695 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 734 740 764 770 437
Size: 30 267 6 24 6 5393
Fragments arranged by size:
5393 267 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 413 598 778 922 1003 2049 413
Size: 185 180 144 81 1046 4090
Fragments arranged by size:
4090 1046 185 180 144 81
BseYI C'CCAG_C
Cuts at: 365 489 1127 2021 3151 3909 4486 4982 365
Size: 124 638 894 1130 758 577 496 1109
Fragments arranged by size:
1130 1109 894 758 638 577 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 1689 558
Size: 1131 4595
BsiEI CG_ry'CG
Cuts at: 104 172 684 3268 3314 4522 104
Size: 68 512 2584 46 1208 1308
Fragments arranged by size:
2584 1308 1208 512 68 46
BsiHKAI G_wGCw'C
Cuts at: 788 2177 2326 2740 3518 3708 4500 788
Size: 1389 149 414 778 190 792 2014
Fragments arranged by size:
2014 1389 792 778 414 190 149
BsmBI CGTCTCn'nnnn_
Cuts at: 2395 2395
Size: 5726
Bsp1286I G_dGCh'C
Cuts at: 235 314 349 713 725 788 920 1301 2177
Size: 79 35 364 12 63 132 381 876
Cuts at: 2177 2326 2421 2740 3351 3444 3518 3708 3770
Size: 149 95 319 611 93 74 190 62
Cuts at: 3770 4500 235
Size: 730 1461
Fragments arranged by size:
1461 876 730 611 381 364 319 190
149 132 95 93 79 74 63 62
35 12
BspEI T'CCGG_A
Cuts at: 4946 4946
Size: 5726
BspHI T'CATG_A
Cuts at: 4902 4902
Size: 5726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2529 2776 2990 3292 3673 2529
Size: 247 214 302 381 4582
Fragments arranged by size:
4582 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 376 4018 4072 5556 5721 50
Size: 326 3642 54 1484 165 55
Fragments arranged by size:
3642 1484 326 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1974 3638 1974
Size: 1664 4062
BsrFI r'CCGG_y
Cuts at: 531 3724 3905 4114 531
Size: 3193 181 209 2143
Fragments arranged by size:
3193 2143 209 181
BsrGI T'GTAC_A
Cuts at: 2006 2006
Size: 5726
BssSI C'ACGA_G
Cuts at: 3997 4355 3997
Size: 358 5368
BstAPI GCAn_nnn'nTGC
Cuts at: 3103 3103
Size: 5726
BstBI TT'CG_AA
Cuts at: 4087 4087
Size: 5726
BstEII G'GTnAC_C
Cuts at: 504 1851 504
Size: 1347 4379
BstYI r'GATC_y
Cuts at: 1 1351 1484 2751 3218 3241 3576 3822 4834
Size: 1350 133 1267 467 23 335 246 1012
Cuts at: 4834 4920 4932 5278 1
Size: 86 12 346 449
Fragments arranged by size:
1350 1267 1012 467 449 346 335 246
133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1898 1898
Size: 5726
BtgI C'CryG_G
Cuts at: 107 547 803 107
Size: 440 256 5030
Fragments arranged by size:
5030 440 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2591 3676 3857 4020 2591
Size: 1085 181 163 4297
Fragments arranged by size:
4297 1085 181 163
BtsI GCAGTG_nn'
Cuts at: 626 1073 2969 626
Size: 447 1896 3383
Fragments arranged by size:
3383 1896 447
DraI TTT'AAA
Cuts at: 1503 2034 2725 1503
Size: 531 691 4504
Fragments arranged by size:
4504 691 531
DrdI GACnn_nn'nnGTC
Cuts at: 974 3432 4290 974
Size: 2458 858 2410
Fragments arranged by size:
2458 2410 858
EaeI y'GGCC_r
Cuts at: 380 681 3311 3485 3876 3903 5248 380
Size: 301 2630 174 391 27 1345 858
Fragments arranged by size:
2630 1345 858 391 301 174 27
EagI C'GGCC_G
Cuts at: 681 3311 681
Size: 2630 3096
EarI CTCTTCn'nnn_
Cuts at: 859 1283 2067 2188 2261 3749 3959 859
Size: 424 784 121 73 1488 210 2626
Fragments arranged by size:
2626 1488 784 424 210 121 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 177 3934 4244 4390 5536 177
Size: 3757 310 146 1146 367
Fragments arranged by size:
3757 1146 367 310 146
EcoICRI GAG'CTC
Cuts at: 786 2324 2738 786
Size: 1538 414 3774
Fragments arranged by size:
3774 1538 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2728 3551 3983 4730 4998 2267
Size: 461 823 432 747 268 2995
Fragments arranged by size:
2995 823 747 461 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 665 665
Size: 5726
EcoO109I rG'GnC_Cy
Cuts at: 215 588 696 722 957 967 1266 2865 215
Size: 373 108 26 235 10 299 1599 3076
Fragments arranged by size:
3076 1599 373 299 235 108 26 10
EcoRI G'AATT_C
Cuts at: 2730 2730
Size: 5726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3745 3745
Size: 5726
FspI TGC'GCA
Cuts at: 943 3507 943
Size: 2564 3162
HaeII r_GCGC'y
Cuts at: 365 1979 3160 3408 4430 5409 365
Size: 1614 1181 248 1022 979 682
Fragments arranged by size:
1614 1181 1022 979 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 1536 1568 2458 2490 2515 2547 2640
Size: 32 228 32 890 32 25 32 93
Cuts at: 2640 2672 3568 3598 3600 3630 1276
Size: 32 896 30 2 30 3372
Fragments arranged by size:
3372 896 890 228 93 32 32 32
32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 602 2765 602
Size: 2163 3563
KasI G'GCGC_C
Cuts at: 361 1975 3156 3404 361
Size: 1614 1181 248 2683
Fragments arranged by size:
2683 1614 1181 248
KpnI G_GTAC'C
Cuts at: 2746 2746
Size: 5726
MfeI C'AATT_G
Cuts at: 2666 2666
Size: 5726
MluI A'CGCG_T
Cuts at: 5159 5159
Size: 5726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 1589 4397 4581 923
Size: 666 2808 184 2068
Fragments arranged by size:
2808 2068 666 184
MscI TGG'CCA
Cuts at: 382 3487 382
Size: 3105 2621
MslI CAynn'nnrTG
Cuts at: 2369 3715 3842 2369
Size: 1346 127 4253
Fragments arranged by size:
4253 1346 127
MspA1I CmG'CkG
Cuts at: 369 549 2025 2423 2450 2837 3151 3511 4524
Size: 180 1476 398 27 387 314 360 1013
Cuts at: 4524 4769 369
Size: 245 1326
Fragments arranged by size:
1476 1326 1013 398 387 360 314 245
180 27
NaeI GCC'GGC
Cuts at: 3907 3907
Size: 5726
NarI GG'CG_CC
Cuts at: 362 1976 3157 3405 362
Size: 1614 1181 248 2683
Fragments arranged by size:
2683 1614 1181 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5726
NdeI CA'TA_TG
Cuts at: 1692 1692
Size: 5726
NgoMIV G'CCGG_C
Cuts at: 3905 3905
Size: 5726
NspI r_CATG'y
Cuts at: 2657 2779 3279 4186 2657
Size: 122 500 907 4197
Fragments arranged by size:
4197 907 500 122
PasI CC'CwG_GG
Cuts at: 972 1021 2436 972
Size: 49 1415 4262
Fragments arranged by size:
4262 1415 49
PciI A'CATG_T
Cuts at: 2653 4182 2653
Size: 1529 4197
PflMI CCAn_nnn'nTGG
Cuts at: 195 496 195
Size: 301 5425
PmeI GTTT'AAAC
Cuts at: 2725 2725
Size: 5726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 695 727 734 766 4865 4897 695
Size: 32 7 32 4099 32 1524
Fragments arranged by size:
4099 1524 32 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 588 696 967 588
Size: 108 271 5347
Fragments arranged by size:
5347 271 108
PspOMI G'GGCC_C
Cuts at: 721 721
Size: 5726
PstI C_TGCA'G
Cuts at: 2466 2540 2773 3458 2466
Size: 74 233 685 4734
Fragments arranged by size:
4734 685 233 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5726
PvuII CAG'CTG
Cuts at: 2025 3151 3511 2025
Size: 1126 360 4240
Fragments arranged by size:
4240 1126 360
RsrII CG'GwC_CG
Cuts at: 3921 3921
Size: 5726
SacI G_AGCT'C
Cuts at: 788 2326 2740 788
Size: 1538 414 3774
Fragments arranged by size:
3774 1538 414
SacII CC_GC'GG
Cuts at: 550 550
Size: 5726
SalI G'TCGA_C
Cuts at: 2763 2763
Size: 5726
SapI GCTCTTCn'nnn_
Cuts at: 2261 3749 3959 2261
Size: 1488 210 4028
Fragments arranged by size:
4028 1488 210
SbfI CC_TGCA'GG
Cuts at: 2540 2773 2540
Size: 233 5493
ScaI AGT'ACT
Cuts at: 5243 5243
Size: 5726
SfcI C'TryA_G
Cuts at: 33 1803 2462 2536 2769 3454 4447 4638 33
Size: 1770 659 74 233 685 993 191 1121
Fragments arranged by size:
1770 1121 993 685 659 233 191 74
SfoI GGC'GCC
Cuts at: 363 1977 3158 3406 363
Size: 1614 1181 248 2683
Fragments arranged by size:
2683 1614 1181 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
SmaI CCC'GGG
Cuts at: 2748 2748
Size: 5726
SmlI C'TyrA_G
Cuts at: 790 934 2124 2229 2881 3796 4288 4550 790
Size: 144 1190 105 652 915 492 262 1966
Fragments arranged by size:
1966 1190 915 652 492 262 144 105
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5726
SphI G_CATG'C
Cuts at: 2779 3279 2779
Size: 500 5226
SspI AAT'ATT
Cuts at: 1566 1566
Size: 5726
StyI C'CwwG_G
Cuts at: 107 960 2642 2860 3214 107
Size: 853 1682 218 354 2619
Fragments arranged by size:
2619 1682 853 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 462 2483 3309 3442 3901 4110 462
Size: 2021 826 133 459 209 2078
Fragments arranged by size:
2078 2021 826 459 209 133
TatI w'GTAC_w
Cuts at: 407 814 2006 5241 407
Size: 407 1192 3235 892
Fragments arranged by size:
3235 1192 892 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 437 469 1660 1692 5243 5275 437
Size: 32 1191 32 3551 32 888
Fragments arranged by size:
3551 1191 888 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2551 3523 2551
Size: 972 4754
XbaI T'CTAG_A
Cuts at: 2757 2757
Size: 5726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3221 3221
Size: 5726
XhoI C'TCGA_G
Cuts at: 790 790
Size: 5726
XmaI C'CCGG_G
Cuts at: 2746 2746
Size: 5726
ZraI GAC'GTC
Cuts at: 2550 2550
Size: 5726
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AscI BcgI BfrBI BglI
BmtI BsiWI BsmI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XmnI