(Circular) (Six-base) MAPSORT of: pF1KB9556.seq Check: 9686 from: 1 to: 5726 pF1KB9556 5726 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2529 2529 Size: 5726 AatII G_ACGT'C Cuts at: 2552 2552 Size: 5726 AccI GT'mk_AC Cuts at: 1718 2150 2764 1718 Size: 432 614 4680 Fragments arranged by size: 4680 614 432 Acc65I G'GTAC_C Cuts at: 2742 2742 Size: 5726 AclI AA'CG_TT Cuts at: 5566 5566 Size: 5726 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 3551 3983 4730 4998 3551 Size: 432 747 268 4279 Fragments arranged by size: 4279 747 432 268 AflII C'TTAA_G Cuts at: 2229 2229 Size: 5726 AflIII A'CryG_T Cuts at: 2568 2653 3078 4182 5159 2568 Size: 85 425 1104 977 3135 Fragments arranged by size: 3135 1104 977 425 85 AgeI A'CCGG_T Cuts at: 4114 4114 Size: 5726 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 734 766 1108 1140 734 Size: 32 342 32 5320 Fragments arranged by size: 5320 342 32 32 AlwNI CAG_nnn'CTG Cuts at: 195 3031 4598 195 Size: 2836 1567 1323 Fragments arranged by size: 2836 1567 1323 ApaI G_GGCC'C Cuts at: 725 725 Size: 5726 ApaLI G'TGCA_C Cuts at: 4496 4496 Size: 5726 ApoI r'AATT_y Cuts at: 1506 2029 2730 5079 5335 1506 Size: 523 701 2349 256 1897 Fragments arranged by size: 2349 1897 701 523 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5726 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5726 AvaI C'yCGr_G Cuts at: 580 790 2746 580 Size: 210 1956 3560 Fragments arranged by size: 3560 1956 210 AvrII C'CTAG_G Cuts at: 960 960 Size: 5726 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1483 1516 1483 Size: 33 5693 BamHI G'GATC_C Cuts at: 2751 2751 Size: 5726 BanI G'GyrC_C Cuts at: 309 361 465 615 1547 1975 2742 3156 3404 Size: 52 104 150 932 428 767 414 248 Cuts at: 3404 3439 309 Size: 35 2596 Fragments arranged by size: 2596 932 767 428 414 248 150 104 52 35 BanII G_rGCy'C Cuts at: 235 349 713 725 788 920 1301 2326 2421 Size: 114 364 12 63 132 381 1025 95 Cuts at: 2421 2740 3770 235 Size: 319 1030 2191 Fragments arranged by size: 2191 1030 1025 381 364 319 132 114 95 63 12 BbeI G_GCGC'C Cuts at: 365 1979 3160 3408 365 Size: 1614 1181 248 2683 Fragments arranged by size: 2683 1614 1181 248 BbsI GAAGACnn'nnnn_ Cuts at: 1160 1805 2114 1160 Size: 645 309 4772 Fragments arranged by size: 4772 645 309 BbvCI CC'TCA_GC Cuts at: 179 179 Size: 5726 BciVI GTATCCnnnnn_n' Cuts at: 1869 3624 4385 5726 1869 Size: 1755 761 1341 1869 Fragments arranged by size: 1869 1755 1341 761 BclI T'GATC_A Cuts at: 3246 3246 Size: 5726 BglII A'GATC_T Cuts at: 1484 3241 5278 1484 Size: 1757 2037 1932 Fragments arranged by size: 2037 1932 1757 BlpI GC'TnA_GC Cuts at: 2513 2838 2513 Size: 325 5401 Bme1580I G_kGCm'C Cuts at: 314 725 3351 3444 4500 314 Size: 411 2626 93 1056 1540 Fragments arranged by size: 2626 1540 1056 411 93 BmgBI CAC'GTC Cuts at: 1011 1052 1011 Size: 41 5685 BmrI ACTGGGnnnn_n' Cuts at: 213 605 2938 3019 3354 5498 213 Size: 392 2333 81 335 2144 441 Fragments arranged by size: 2333 2144 441 392 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 298 4093 298 Size: 3795 1931 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 2728 2267 Size: 461 5265 Bpu10I CC'TnA_GC Cuts at: 179 179 Size: 5726 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 919 2109 2902 3781 4273 4571 919 Size: 1190 793 879 492 298 2074 Fragments arranged by size: 2074 1190 879 793 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1031 2704 5430 1031 Size: 1673 2726 1327 Fragments arranged by size: 2726 1673 1327 BsaAI yAC'GTr Cuts at: 3079 3079 Size: 5726 BsaBI GATnn'nnATC Cuts at: 6 3240 5277 6 Size: 3234 2037 455 Fragments arranged by size: 3234 2037 455 BsaHI Gr'CG_yC Cuts at: 362 1976 2549 3157 3405 4107 5603 362 Size: 1614 573 608 248 702 1496 485 Fragments arranged by size: 1614 1496 702 608 573 485 248 BsaWI w'CCGG_w Cuts at: 2915 3436 4114 4388 4535 4946 2915 Size: 521 678 274 147 411 3695 Fragments arranged by size: 3695 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 437 467 734 740 764 770 437 Size: 30 267 6 24 6 5393 Fragments arranged by size: 5393 267 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 413 598 778 922 1003 2049 413 Size: 185 180 144 81 1046 4090 Fragments arranged by size: 4090 1046 185 180 144 81 BseYI C'CCAG_C Cuts at: 365 489 1127 2021 3151 3909 4486 4982 365 Size: 124 638 894 1130 758 577 496 1109 Fragments arranged by size: 1130 1109 894 758 638 577 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 558 1689 558 Size: 1131 4595 BsiEI CG_ry'CG Cuts at: 104 172 684 3268 3314 4522 104 Size: 68 512 2584 46 1208 1308 Fragments arranged by size: 2584 1308 1208 512 68 46 BsiHKAI G_wGCw'C Cuts at: 788 2177 2326 2740 3518 3708 4500 788 Size: 1389 149 414 778 190 792 2014 Fragments arranged by size: 2014 1389 792 778 414 190 149 BsmBI CGTCTCn'nnnn_ Cuts at: 2395 2395 Size: 5726 Bsp1286I G_dGCh'C Cuts at: 235 314 349 713 725 788 920 1301 2177 Size: 79 35 364 12 63 132 381 876 Cuts at: 2177 2326 2421 2740 3351 3444 3518 3708 3770 Size: 149 95 319 611 93 74 190 62 Cuts at: 3770 4500 235 Size: 730 1461 Fragments arranged by size: 1461 876 730 611 381 364 319 190 149 132 95 93 79 74 63 62 35 12 BspEI T'CCGG_A Cuts at: 4946 4946 Size: 5726 BspHI T'CATG_A Cuts at: 4902 4902 Size: 5726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2529 2776 2990 3292 3673 2529 Size: 247 214 302 381 4582 Fragments arranged by size: 4582 381 302 247 214 BsrBI CCG'CTC Cuts at: 50 376 4018 4072 5556 5721 50 Size: 326 3642 54 1484 165 55 Fragments arranged by size: 3642 1484 326 165 55 54 BsrDI GCAATG_nn' Cuts at: 1974 3638 1974 Size: 1664 4062 BsrFI r'CCGG_y Cuts at: 531 3724 3905 4114 531 Size: 3193 181 209 2143 Fragments arranged by size: 3193 2143 209 181 BsrGI T'GTAC_A Cuts at: 2006 2006 Size: 5726 BssSI C'ACGA_G Cuts at: 3997 4355 3997 Size: 358 5368 BstAPI GCAn_nnn'nTGC Cuts at: 3103 3103 Size: 5726 BstBI TT'CG_AA Cuts at: 4087 4087 Size: 5726 BstEII G'GTnAC_C Cuts at: 504 1851 504 Size: 1347 4379 BstYI r'GATC_y Cuts at: 1 1351 1484 2751 3218 3241 3576 3822 4834 Size: 1350 133 1267 467 23 335 246 1012 Cuts at: 4834 4920 4932 5278 1 Size: 86 12 346 449 Fragments arranged by size: 1350 1267 1012 467 449 346 335 246 133 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1898 1898 Size: 5726 BtgI C'CryG_G Cuts at: 107 547 803 107 Size: 440 256 5030 Fragments arranged by size: 5030 440 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2591 3676 3857 4020 2591 Size: 1085 181 163 4297 Fragments arranged by size: 4297 1085 181 163 BtsI GCAGTG_nn' Cuts at: 626 1073 2969 626 Size: 447 1896 3383 Fragments arranged by size: 3383 1896 447 DraI TTT'AAA Cuts at: 1503 2034 2725 1503 Size: 531 691 4504 Fragments arranged by size: 4504 691 531 DrdI GACnn_nn'nnGTC Cuts at: 974 3432 4290 974 Size: 2458 858 2410 Fragments arranged by size: 2458 2410 858 EaeI y'GGCC_r Cuts at: 380 681 3311 3485 3876 3903 5248 380 Size: 301 2630 174 391 27 1345 858 Fragments arranged by size: 2630 1345 858 391 301 174 27 EagI C'GGCC_G Cuts at: 681 3311 681 Size: 2630 3096 EarI CTCTTCn'nnn_ Cuts at: 859 1283 2067 2188 2261 3749 3959 859 Size: 424 784 121 73 1488 210 2626 Fragments arranged by size: 2626 1488 784 424 210 121 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 177 3934 4244 4390 5536 177 Size: 3757 310 146 1146 367 Fragments arranged by size: 3757 1146 367 310 146 EcoICRI GAG'CTC Cuts at: 786 2324 2738 786 Size: 1538 414 3774 Fragments arranged by size: 3774 1538 414 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 2728 3551 3983 4730 4998 2267 Size: 461 823 432 747 268 2995 Fragments arranged by size: 2995 823 747 461 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 665 665 Size: 5726 EcoO109I rG'GnC_Cy Cuts at: 215 588 696 722 957 967 1266 2865 215 Size: 373 108 26 235 10 299 1599 3076 Fragments arranged by size: 3076 1599 373 299 235 108 26 10 EcoRI G'AATT_C Cuts at: 2730 2730 Size: 5726 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3745 3745 Size: 5726 FspI TGC'GCA Cuts at: 943 3507 943 Size: 2564 3162 HaeII r_GCGC'y Cuts at: 365 1979 3160 3408 4430 5409 365 Size: 1614 1181 248 1022 979 682 Fragments arranged by size: 1614 1181 1022 979 682 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1276 1308 1536 1568 2458 2490 2515 2547 2640 Size: 32 228 32 890 32 25 32 93 Cuts at: 2640 2672 3568 3598 3600 3630 1276 Size: 32 896 30 2 30 3372 Fragments arranged by size: 3372 896 890 228 93 32 32 32 32 32 30 30 25 2 HincII GTy'rAC Cuts at: 602 2765 602 Size: 2163 3563 KasI G'GCGC_C Cuts at: 361 1975 3156 3404 361 Size: 1614 1181 248 2683 Fragments arranged by size: 2683 1614 1181 248 KpnI G_GTAC'C Cuts at: 2746 2746 Size: 5726 MfeI C'AATT_G Cuts at: 2666 2666 Size: 5726 MluI A'CGCG_T Cuts at: 5159 5159 Size: 5726 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 923 1589 4397 4581 923 Size: 666 2808 184 2068 Fragments arranged by size: 2808 2068 666 184 MscI TGG'CCA Cuts at: 382 3487 382 Size: 3105 2621 MslI CAynn'nnrTG Cuts at: 2369 3715 3842 2369 Size: 1346 127 4253 Fragments arranged by size: 4253 1346 127 MspA1I CmG'CkG Cuts at: 369 549 2025 2423 2450 2837 3151 3511 4524 Size: 180 1476 398 27 387 314 360 1013 Cuts at: 4524 4769 369 Size: 245 1326 Fragments arranged by size: 1476 1326 1013 398 387 360 314 245 180 27 NaeI GCC'GGC Cuts at: 3907 3907 Size: 5726 NarI GG'CG_CC Cuts at: 362 1976 3157 3405 362 Size: 1614 1181 248 2683 Fragments arranged by size: 2683 1614 1181 248 NcoI C'CATG_G Cuts at: 107 107 Size: 5726 NdeI CA'TA_TG Cuts at: 1692 1692 Size: 5726 NgoMIV G'CCGG_C Cuts at: 3905 3905 Size: 5726 NspI r_CATG'y Cuts at: 2657 2779 3279 4186 2657 Size: 122 500 907 4197 Fragments arranged by size: 4197 907 500 122 PasI CC'CwG_GG Cuts at: 972 1021 2436 972 Size: 49 1415 4262 Fragments arranged by size: 4262 1415 49 PciI A'CATG_T Cuts at: 2653 4182 2653 Size: 1529 4197 PflMI CCAn_nnn'nTGG Cuts at: 195 496 195 Size: 301 5425 PmeI GTTT'AAAC Cuts at: 2725 2725 Size: 5726 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 695 727 734 766 4865 4897 695 Size: 32 7 32 4099 32 1524 Fragments arranged by size: 4099 1524 32 32 32 7 PpuMI rG'GwC_Cy Cuts at: 588 696 967 588 Size: 108 271 5347 Fragments arranged by size: 5347 271 108 PspOMI G'GGCC_C Cuts at: 721 721 Size: 5726 PstI C_TGCA'G Cuts at: 2466 2540 2773 3458 2466 Size: 74 233 685 4734 Fragments arranged by size: 4734 685 233 74 PvuI CG_AT'CG Cuts at: 104 104 Size: 5726 PvuII CAG'CTG Cuts at: 2025 3151 3511 2025 Size: 1126 360 4240 Fragments arranged by size: 4240 1126 360 RsrII CG'GwC_CG Cuts at: 3921 3921 Size: 5726 SacI G_AGCT'C Cuts at: 788 2326 2740 788 Size: 1538 414 3774 Fragments arranged by size: 3774 1538 414 SacII CC_GC'GG Cuts at: 550 550 Size: 5726 SalI G'TCGA_C Cuts at: 2763 2763 Size: 5726 SapI GCTCTTCn'nnn_ Cuts at: 2261 3749 3959 2261 Size: 1488 210 4028 Fragments arranged by size: 4028 1488 210 SbfI CC_TGCA'GG Cuts at: 2540 2773 2540 Size: 233 5493 ScaI AGT'ACT Cuts at: 5243 5243 Size: 5726 SfcI C'TryA_G Cuts at: 33 1803 2462 2536 2769 3454 4447 4638 33 Size: 1770 659 74 233 685 993 191 1121 Fragments arranged by size: 1770 1121 993 685 659 233 191 74 SfoI GGC'GCC Cuts at: 363 1977 3158 3406 363 Size: 1614 1181 248 2683 Fragments arranged by size: 2683 1614 1181 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5726 SmaI CCC'GGG Cuts at: 2748 2748 Size: 5726 SmlI C'TyrA_G Cuts at: 790 934 2124 2229 2881 3796 4288 4550 790 Size: 144 1190 105 652 915 492 262 1966 Fragments arranged by size: 1966 1190 915 652 492 262 144 105 SpeI A'CTAG_T Cuts at: 67 67 Size: 5726 SphI G_CATG'C Cuts at: 2779 3279 2779 Size: 500 5226 SspI AAT'ATT Cuts at: 1566 1566 Size: 5726 StyI C'CwwG_G Cuts at: 107 960 2642 2860 3214 107 Size: 853 1682 218 354 2619 Fragments arranged by size: 2619 1682 853 354 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 462 2483 3309 3442 3901 4110 462 Size: 2021 826 133 459 209 2078 Fragments arranged by size: 2078 2021 826 459 209 133 TatI w'GTAC_w Cuts at: 407 814 2006 5241 407 Size: 407 1192 3235 892 Fragments arranged by size: 3235 1192 892 407 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 437 469 1660 1692 5243 5275 437 Size: 32 1191 32 3551 32 888 Fragments arranged by size: 3551 1191 888 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2551 3523 2551 Size: 972 4754 XbaI T'CTAG_A Cuts at: 2757 2757 Size: 5726 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3221 3221 Size: 5726 XhoI C'TCGA_G Cuts at: 790 790 Size: 5726 XmaI C'CCGG_G Cuts at: 2746 2746 Size: 5726 ZraI GAC'GTC Cuts at: 2550 2550 Size: 5726 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AhdI AleI AlfI AscI BcgI BfrBI BglI BmtI BsiWI BsmI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI