Restriction map for
pF1KB9553
(Circular) (Six-base) MAPSORT of: pF1KB9553.seq Check: 4368 from: 1 to: 3896
pF1KB9553 3896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 621 621
Size: 3896
AatII G_ACGT'C
Cuts at: 861 861
Size: 3896
AccI GT'mk_AC
Cuts at: 805 934 805
Size: 129 3767
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 890 3736 890
Size: 2846 1050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 679 1721 2153 2900 3168 560
Size: 119 1042 432 747 268 1288
Fragments arranged by size:
1288 1042 747 432 268 119
AfeI AGC'GCT
Cuts at: 514 514
Size: 3896
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 213 708 777 1201 2768 213
Size: 495 69 424 1567 1341
Fragments arranged by size:
1567 1341 495 424 69
ApaLI G'TGCA_C
Cuts at: 617 2666 617
Size: 2049 1847
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 883 20
Size: 863 3033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 313 916 313
Size: 603 3293
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 225 912 1326 1574 1609 225
Size: 687 414 248 35 2512
Fragments arranged by size:
2512 687 414 248 35
BanII G_rGCy'C
Cuts at: 198 313 796 910 1940 198
Size: 115 483 114 1030 2154
Fragments arranged by size:
2154 1030 483 115 114
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbvCI CC'TCA_GC
Cuts at: 328 328
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 208 208
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 621 1521 1614 2670 621
Size: 900 93 1056 1847
Fragments arranged by size:
1847 1056 900 93
BmrI ACTGGGnnnn_n'
Cuts at: 636 738 814 1108 1189 1524 3668 636
Size: 102 76 294 81 335 2144 864
Fragments arranged by size:
2144 864 335 294 102 81 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 807 640
Size: 167 3729
Bpu10I CC'TnA_GC
Cuts at: 307 328 307
Size: 21 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 616 1249 616
Size: 633 3263
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 858 1327 1575 2277 3773 858
Size: 469 248 702 1496 981
Fragments arranged by size:
1496 981 702 469 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 345 559 804 331
Size: 14 214 245 3423
Fragments arranged by size:
3423 245 214 14
BseYI C'CCAG_C
Cuts at: 1321 2079 2656 3152 1321
Size: 758 577 496 2065
Fragments arranged by size:
2065 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 140 203 1438 1484 2692 104
Size: 36 63 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 63 46 36
BsiHKAI G_wGCw'C
Cuts at: 621 796 910 1688 1878 2670 621
Size: 175 114 778 190 792 1847
Fragments arranged by size:
1847 792 778 190 175 114
BsmI GAATG_Cn'
Cuts at: 724 724
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 198 313 621 796 910 1521 1614 1688 1878
Size: 115 308 175 114 611 93 74 190
Cuts at: 1878 1940 2670 198
Size: 62 730 1424
Fragments arranged by size:
1424 730 611 308 190 175 115 114
93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 621 946 1160 1462 1843 621
Size: 325 214 302 381 2674
Fragments arranged by size:
2674 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 219 263 2188 2242 3726 3891 50
Size: 169 44 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 169 165 55 54 44
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 202 1894 2075 2284 202
Size: 1692 181 209 1814
Fragments arranged by size:
1814 1692 209 181
BssHII G'CGCG_C
Cuts at: 280 280
Size: 3896
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 633 1273 633
Size: 640 3256
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 670 107
Size: 563 3333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 137 200 204 581 1481 1655 2046 2073 3418
Size: 63 4 377 900 174 391 27 1345
Cuts at: 3418 137
Size: 615
Fragments arranged by size:
1345 900 615 391 377 174 63 27
4
EagI C'GGCC_G
Cuts at: 137 200 1481 137
Size: 63 1281 2552
Fragments arranged by size:
2552 1281 63
EarI CTCTTCn'nnn_
Cuts at: 422 536 698 1919 2129 422
Size: 114 162 1221 210 2189
Fragments arranged by size:
2189 1221 210 162 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 565 2104 2414 2560 3706 174
Size: 391 1539 310 146 1146 364
Fragments arranged by size:
1539 1146 391 364 310 146
EcoICRI GAG'CTC
Cuts at: 794 908 794
Size: 114 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 640 679 807 1721 2153 2900 3168 560
Size: 80 39 128 914 432 747 268 1288
Fragments arranged by size:
1288 914 747 432 268 128 80 39
EcoO109I rG'GnC_Cy
Cuts at: 146 210 816 1035 146
Size: 64 606 219 3007
Fragments arranged by size:
3007 606 219 64
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
EcoRV GAT'ATC
Cuts at: 713 713
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 178 1915 178
Size: 1737 2159
FseI GG_CCGG'CC
Cuts at: 206 206
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 297 328 516 1330 1578 2600 3579 297
Size: 31 188 814 248 1022 979 614
Fragments arranged by size:
1022 979 814 614 248 188 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 690 722 1738 1768 1770 1800 281
Size: 32 377 32 1016 30 2 30 2377
Fragments arranged by size:
2377 1016 377 32 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 112 1885 2012 112
Size: 1773 127 1996
Fragments arranged by size:
1996 1773 127
MspA1I CmG'CkG
Cuts at: 1007 1321 1681 2694 2939 1007
Size: 314 360 1013 245 1964
Fragments arranged by size:
1964 1013 360 314 245
NaeI GCC'GGC
Cuts at: 204 2077 204
Size: 1873 2023
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 670 107
Size: 563 3333
NgoMIV G'CCGG_C
Cuts at: 202 2075 202
Size: 1873 2023
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 146 816 146
Size: 670 3226
PstI C_TGCA'G
Cuts at: 629 943 1628 629
Size: 314 685 2897
Fragments arranged by size:
2897 685 314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 796 910 796
Size: 114 3782
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SanDI GG'GwC_CC
Cuts at: 146 146
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 625 939 1624 2617 2808 33
Size: 592 314 685 993 191 1121
Fragments arranged by size:
1121 993 685 592 314 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 208 208
Size: 3896
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 107 557 670 1030 1384 107
Size: 450 113 360 354 2619
Fragments arranged by size:
2619 450 360 354 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 744 1693 744
Size: 949 2947
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1391 114
Size: 1277 2619
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
ZraI GAC'GTC
Cuts at: 859 859
Size: 3896
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI